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The map label for this gene is irlS [H]

Identifier: 146340890

GI number: 146340890

Start: 4137147

End: 4138745

Strand: Direct

Name: irlS [H]

Synonym: BRADO3956

Alternate gene names: 146340890

Gene position: 4137147-4138745 (Clockwise)

Preceding gene: 146340889

Following gene: 146340891

Centisome position: 55.48

GC content: 66.67

Gene sequence:

>1599_bases
ATGATCGCACCCAAGCCGAAGAAGAAATGGCGGCCTTCGCTCGGACAGATCGTGTTCGGCGTGCTGGCGCTCGTCGCCAC
CCTGCCGCTGGTCGGGCTTTTCTTCTTCCGCATCTACGACAATCAGCTGATCCGCCAGACCCAGGCCGAGCTGATCGCGC
AGAGCCGCGTGCTGGCCGCCGTCTATGCCGAGGAGGTCACCGCACGGCTCGCCGCCGGAATTCCGCTGGGAGCCGAGATC
CCACCGGACGCCCGCCCGGACAGCTCCGACGAAGTGACGCCGATCCGTCCGCTGCTCGACCTGCTGGCCAATGATCTCTT
CGTTCGCCGTCCCGACGCTCGCGATGCGGCCCAGCCCGCCGACCCTGCTTATGTCGCGATCGGCGCCCGGCTGACCCCGG
TGATCCGCGAAACCCAAAAGGTGACGCTGGCCGGCTTCCGCGTGCTCGATCCGCAGGGCGTCGTGATCGCCGGACGCGAC
GAGATGGGTAAATCGCTGGCGCATATCGAGGAGGTCGCGGCCGCGTTGAAAGGAAAATACAGCGCCGCGCTGCGCATCCG
CATTCCGGACAAGGCGCCACCGCCGATCTACTCGTTCAGCCGCGGCGTCGGCGTCCATGTGTTCTCCGCAATGCCGGTGA
TCGTCAACGACCACGTCGCCGGCGTGATCTATACGTCGCGGACGCCGCGCAACATCTTCGAGCACCTTTATGTCGAGCGC
GGCAAGTTCATCGCGGCCGCATCCGCCATCATTCTCACGACGCTGCTGGTCGGCCTCATCGTGGCGCGCACGATCACCAG
GCCGATGCGCGAGCTGGTCGACCGCGCCGGACAGATCGGCCGCAACGATCCCGCGGGCTTCCGGCCGCTCGCCCATTACG
GCACGCGCGACTTTGCCGAACTGGCAGAGCACTTCTTCGCCATGGCCGAGCAGCTCGCGCAGCGCTCCAGCTATGTCAGG
AACTTCTCCGCCCATCTGACCCATGAGCTGAAATCGCCGCTGACCTCGATCAAGGGCGCCGCGGAGCTGCTCATGGATTC
TCTCCACGCCACGTCGGGGCAGTTGACGCGCGCCGAGCAGGAACGGTTTCTCGGAAACATTCTCGGCGACGCGGATCGTC
TCGAGCGGATGGCGCAACGCCTGCGTGAGCTGGCGCGCGCCGAAGCCCTGCAGCAGAGCGAGACCAGCAGTCCGGCCGAT
GTGATCCCCGCGCTGCGCGAGCGATTTCCGGCGATCCAGATCGAGCTGCGCGGCGACCTGAACCACCGCGTCATTCTGTC
CGGCGAGACGCTCCTGATCGTGCTCACGCATCTTGCCGACAACGCCATGCGCCACGGGGCGCGCACGATCTGGCTGGAGG
CGGCCAACCGCAGCAATCAACTGGCCCTGAGCGTCAGCAATGACGGCCATCCGATCTCGCCGGAGAACCGCGCGCGGATC
TTCGATGCCTTCTTCACCACGCGCCGCGACAGCGGCGGCACCGGGATGGGGCTTTCGATCGTGCGCGCCGTCATGACAAG
CCATGGCGGAGCCATCGACCTCCTGCCCGATGCGCCGGAGGTCAGCTTCGAGCTGCGGCTGCCGGCCGCTGAACGCTGA

Upstream 100 bases:

>100_bases
ACATCCGCAACATCCGCGCCAAGCTCGCGACGGCCGGCTGCGACGACGCGATCGAGACCGTGCATGGCGTCGGCTTCCGG
CTGGGGCGCTGCGGCGCGCC

Downstream 100 bases:

>100_bases
ACGCTGGATCAAATATACCTCAAATAGAATCTGGAGCGGCGAATTCAGCCAAAGTTGCAGATCGCGCGACGTCCGGCTGT
GGATGCCGCGACATGCCGCC

Product: putative two-component system histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MIAPKPKKKWRPSLGQIVFGVLALVATLPLVGLFFFRIYDNQLIRQTQAELIAQSRVLAAVYAEEVTARLAAGIPLGAEI
PPDARPDSSDEVTPIRPLLDLLANDLFVRRPDARDAAQPADPAYVAIGARLTPVIRETQKVTLAGFRVLDPQGVVIAGRD
EMGKSLAHIEEVAAALKGKYSAALRIRIPDKAPPPIYSFSRGVGVHVFSAMPVIVNDHVAGVIYTSRTPRNIFEHLYVER
GKFIAAASAIILTTLLVGLIVARTITRPMRELVDRAGQIGRNDPAGFRPLAHYGTRDFAELAEHFFAMAEQLAQRSSYVR
NFSAHLTHELKSPLTSIKGAAELLMDSLHATSGQLTRAEQERFLGNILGDADRLERMAQRLRELARAEALQQSETSSPAD
VIPALRERFPAIQIELRGDLNHRVILSGETLLIVLTHLADNAMRHGARTIWLEAANRSNQLALSVSNDGHPISPENRARI
FDAFFTTRRDSGGTGMGLSIVRAVMTSHGGAIDLLPDAPEVSFELRLPAAER

Sequences:

>Translated_532_residues
MIAPKPKKKWRPSLGQIVFGVLALVATLPLVGLFFFRIYDNQLIRQTQAELIAQSRVLAAVYAEEVTARLAAGIPLGAEI
PPDARPDSSDEVTPIRPLLDLLANDLFVRRPDARDAAQPADPAYVAIGARLTPVIRETQKVTLAGFRVLDPQGVVIAGRD
EMGKSLAHIEEVAAALKGKYSAALRIRIPDKAPPPIYSFSRGVGVHVFSAMPVIVNDHVAGVIYTSRTPRNIFEHLYVER
GKFIAAASAIILTTLLVGLIVARTITRPMRELVDRAGQIGRNDPAGFRPLAHYGTRDFAELAEHFFAMAEQLAQRSSYVR
NFSAHLTHELKSPLTSIKGAAELLMDSLHATSGQLTRAEQERFLGNILGDADRLERMAQRLRELARAEALQQSETSSPAD
VIPALRERFPAIQIELRGDLNHRVILSGETLLIVLTHLADNAMRHGARTIWLEAANRSNQLALSVSNDGHPISPENRARI
FDAFFTTRRDSGGTGMGLSIVRAVMTSHGGAIDLLPDAPEVSFELRLPAAER
>Mature_532_residues
MIAPKPKKKWRPSLGQIVFGVLALVATLPLVGLFFFRIYDNQLIRQTQAELIAQSRVLAAVYAEEVTARLAAGIPLGAEI
PPDARPDSSDEVTPIRPLLDLLANDLFVRRPDARDAAQPADPAYVAIGARLTPVIRETQKVTLAGFRVLDPQGVVIAGRD
EMGKSLAHIEEVAAALKGKYSAALRIRIPDKAPPPIYSFSRGVGVHVFSAMPVIVNDHVAGVIYTSRTPRNIFEHLYVER
GKFIAAASAIILTTLLVGLIVARTITRPMRELVDRAGQIGRNDPAGFRPLAHYGTRDFAELAEHFFAMAEQLAQRSSYVR
NFSAHLTHELKSPLTSIKGAAELLMDSLHATSGQLTRAEQERFLGNILGDADRLERMAQRLRELARAEALQQSETSSPAD
VIPALRERFPAIQIELRGDLNHRVILSGETLLIVLTHLADNAMRHGARTIWLEAANRSNQLALSVSNDGHPISPENRARI
FDAFFTTRRDSGGTGMGLSIVRAVMTSHGGAIDLLPDAPEVSFELRLPAAER

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=310, Percent_Identity=26.7741935483871, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786783, Length=334, Percent_Identity=27.5449101796407, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=22.7941176470588, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790861, Length=380, Percent_Identity=25.7894736842105, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=258, Percent_Identity=25.968992248062, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=232, Percent_Identity=27.1551724137931, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=27.8538812785388, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58195; Mature: 58195

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPKPKKKWRPSLGQIVFGVLALVATLPLVGLFFFRIYDNQLIRQTQAELIAQSRVLAA
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYAEEVTARLAAGIPLGAEIPPDARPDSSDEVTPIRPLLDLLANDLFVRRPDARDAAQPA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCC
DPAYVAIGARLTPVIRETQKVTLAGFRVLDPQGVVIAGRDEMGKSLAHIEEVAAALKGKY
CCCEEEECCHHHHHHHHHHHEEEECEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
SAALRIRIPDKAPPPIYSFSRGVGVHVFSAMPVIVNDHVAGVIYTSRTPRNIFEHLYVER
EEEEEEECCCCCCCCCHHCCCCCCEEEHHCCCEEECCCEEEEEEECCCHHHHHHHHHHHC
GKFIAAASAIILTTLLVGLIVARTITRPMRELVDRAGQIGRNDPAGFRPLAHYGTRDFAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHH
LAEHFFAMAEQLAQRSSYVRNFSAHLTHELKSPLTSIKGAAELLMDSLHATSGQLTRAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ERFLGNILGDADRLERMAQRLRELARAEALQQSETSSPADVIPALRERFPAIQIELRGDL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCC
NHRVILSGETLLIVLTHLADNAMRHGARTIWLEAANRSNQLALSVSNDGHPISPENRARI
CCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHH
FDAFFTTRRDSGGTGMGLSIVRAVMTSHGGAIDLLPDAPEVSFELRLPAAER
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MIAPKPKKKWRPSLGQIVFGVLALVATLPLVGLFFFRIYDNQLIRQTQAELIAQSRVLAA
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYAEEVTARLAAGIPLGAEIPPDARPDSSDEVTPIRPLLDLLANDLFVRRPDARDAAQPA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCC
DPAYVAIGARLTPVIRETQKVTLAGFRVLDPQGVVIAGRDEMGKSLAHIEEVAAALKGKY
CCCEEEECCHHHHHHHHHHHEEEECEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
SAALRIRIPDKAPPPIYSFSRGVGVHVFSAMPVIVNDHVAGVIYTSRTPRNIFEHLYVER
EEEEEEECCCCCCCCCHHCCCCCCEEEHHCCCEEECCCEEEEEEECCCHHHHHHHHHHHC
GKFIAAASAIILTTLLVGLIVARTITRPMRELVDRAGQIGRNDPAGFRPLAHYGTRDFAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHH
LAEHFFAMAEQLAQRSSYVRNFSAHLTHELKSPLTSIKGAAELLMDSLHATSGQLTRAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ERFLGNILGDADRLERMAQRLRELARAEALQQSETSSPADVIPALRERFPAIQIELRGDL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCC
NHRVILSGETLLIVLTHLADNAMRHGARTIWLEAANRSNQLALSVSNDGHPISPENRARI
CCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHH
FDAFFTTRRDSGGTGMGLSIVRAVMTSHGGAIDLLPDAPEVSFELRLPAAER
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]