Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 146340889

GI number: 146340889

Start: 4136455

End: 4137150

Strand: Direct

Name: phoB [H]

Synonym: BRADO3955

Alternate gene names: 146340889

Gene position: 4136455-4137150 (Clockwise)

Preceding gene: 146340888

Following gene: 146340890

Centisome position: 55.47

GC content: 67.39

Gene sequence:

>696_bases
GTGTCCCCCCGAATCCTCATTGTCGACGACGACCCGCATATCCGGGAGGTGGTGCGCGTCGCCTTGAGCAAGGCCGGCAT
GGCCGTGACGGAGGCCGCCGACGGACGGGCCGCGTTGGCCAGGTTCGCCGACGATCGGCCAGACCTGATCGTGCTGGACG
TCGGCATGCCCGAGTTCGATGGGCTCGAAGTCTGCCGGCGCATCCGCAGGACGTCGCAGGTGCCGATCCTGTTTTTGTCG
GCGCGTGACGAGGAGATCGACCGCATCCTCGGTCTGGAGATCGGCGGTGACGACTACGTCACCAAGCCGTTCAGTCCCCG
AGAGCTCGTTGCCCGCGTCAACGTCATTCTACGCCGTGTCGCAACGGGCCATCGCTCCGACGAGGCGGAGGAGCTGGTTC
ACGGCCGGCTTCGGCTCGATCCGCAGCAGCATCTGGCGCGGTTCGGCGACACGCCGCTCACGCTCACCGCGCTGGAGTTC
AGCATCGTTCGCACGCTGCTGACGCGCCCCGCCGTCGTGTTCAGCCGCGAGCAGATCATGAGCGCCGCTTATCAGCTCAA
TATCCAGGTCTCCGACCGGACCATCGACAGCCACATCCGCAACATCCGCGCCAAGCTCGCGACGGCCGGCTGCGACGACG
CGATCGAGACCGTGCATGGCGTCGGCTTCCGGCTGGGGCGCTGCGGCGCGCCATGA

Upstream 100 bases:

>100_bases
GGCGCTCATGGAGCTTTCGTGGCTGGCGCTTGCAGCGCTATCTCGATACACATCCTCTGTCGTCGAACCCCAGCTGATGT
CTCGCCGCGGAGCCCAAATC

Downstream 100 bases:

>100_bases
TCGCACCCAAGCCGAAGAAGAAATGGCGGCCTTCGCTCGGACAGATCGTGTTCGGCGTGCTGGCGCTCGTCGCCACCCTG
CCGCTGGTCGGGCTTTTCTT

Product: OmpR family two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSPRILIVDDDPHIREVVRVALSKAGMAVTEAADGRAALARFADDRPDLIVLDVGMPEFDGLEVCRRIRRTSQVPILFLS
ARDEEIDRILGLEIGGDDYVTKPFSPRELVARVNVILRRVATGHRSDEAEELVHGRLRLDPQQHLARFGDTPLTLTALEF
SIVRTLLTRPAVVFSREQIMSAAYQLNIQVSDRTIDSHIRNIRAKLATAGCDDAIETVHGVGFRLGRCGAP

Sequences:

>Translated_231_residues
MSPRILIVDDDPHIREVVRVALSKAGMAVTEAADGRAALARFADDRPDLIVLDVGMPEFDGLEVCRRIRRTSQVPILFLS
ARDEEIDRILGLEIGGDDYVTKPFSPRELVARVNVILRRVATGHRSDEAEELVHGRLRLDPQQHLARFGDTPLTLTALEF
SIVRTLLTRPAVVFSREQIMSAAYQLNIQVSDRTIDSHIRNIRAKLATAGCDDAIETVHGVGFRLGRCGAP
>Mature_230_residues
SPRILIVDDDPHIREVVRVALSKAGMAVTEAADGRAALARFADDRPDLIVLDVGMPEFDGLEVCRRIRRTSQVPILFLSA
RDEEIDRILGLEIGGDDYVTKPFSPRELVARVNVILRRVATGHRSDEAEELVHGRLRLDPQQHLARFGDTPLTLTALEFS
IVRTLLTRPAVVFSREQIMSAAYQLNIQVSDRTIDSHIRNIRAKLATAGCDDAIETVHGVGFRLGRCGAP

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=39.7379912663755, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=43.5555555555556, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=40.8071748878924, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=35.5555555555556, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=35.2678571428571, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=32.7659574468085, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=33.6363636363636, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=34.0807174887892, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI2367329, Length=210, Percent_Identity=39.0476190476191, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1786911, Length=202, Percent_Identity=35.1485148514851, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=33.6206896551724, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.5315315315315, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=31.390134529148, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI145693140, Length=228, Percent_Identity=27.6315789473684, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=36.7521367521368, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=33.6363636363636, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=31.9672131147541, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=206, Percent_Identity=30.0970873786408, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=35.6435643564356, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25609; Mature: 25478

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRILIVDDDPHIREVVRVALSKAGMAVTEAADGRAALARFADDRPDLIVLDVGMPEFD
CCCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCEEEEECCCCCCC
GLEVCRRIRRTSQVPILFLSARDEEIDRILGLEIGGDDYVTKPFSPRELVARVNVILRRV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ATGHRSDEAEELVHGRLRLDPQQHLARFGDTPLTLTALEFSIVRTLLTRPAVVFSREQIM
HCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
SAAYQLNIQVSDRTIDSHIRNIRAKLATAGCDDAIETVHGVGFRLGRCGAP
HHHHEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SPRILIVDDDPHIREVVRVALSKAGMAVTEAADGRAALARFADDRPDLIVLDVGMPEFD
CCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCEEEEECCCCCCC
GLEVCRRIRRTSQVPILFLSARDEEIDRILGLEIGGDDYVTKPFSPRELVARVNVILRRV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ATGHRSDEAEELVHGRLRLDPQQHLARFGDTPLTLTALEFSIVRTLLTRPAVVFSREQIM
HCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
SAAYQLNIQVSDRTIDSHIRNIRAKLATAGCDDAIETVHGVGFRLGRCGAP
HHHHEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]