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The map label for this gene is lipA [H]

Identifier: 146340818

GI number: 146340818

Start: 4052338

End: 4053297

Strand: Reverse

Name: lipA [H]

Synonym: BRADO3879

Alternate gene names: 146340818

Gene position: 4053297-4052338 (Counterclockwise)

Preceding gene: 146340820

Following gene: 146340817

Centisome position: 54.36

GC content: 66.88

Gene sequence:

>960_bases
ATGGTCACCATCGTCGACATCAACGCGAACACGCCGCTGCGCCCCCGCCACCCCGAAAAGGTGAACCGGCCGGACTCGGT
GTCGCCGGCCAAGCCCGCCTGGATCCGCGTCAAGGCGCCGACCACCCGCGGCTATGCCGACACCCGCAACATCGTCCGCG
AGAACGGCCTCGTCACCGTGTGCGAGGAGGCCGGCTGCCCGAACATCGGCGAGTGCTGGGACAAGAAGCACGCCACCTTC
ATGATCATGGGCGACACCTGCACCCGGGCCTGCGCCTTCTGCAACGTCAAGACCGGCATGCCCGCCGCGCTCGACGCCGG
CGAGCCGGAGCACGTGGCGGAGGCGACCTTCAAGCTCGGCCTCGCCCATGTCGTCGTCACATCGGTCGACCGCGACGATC
TTGCCGACGGGGGCGCCGCGCACATCGCAGAAACCATCCGCGCGATCCGCGCGAAGTGCCCGACCACCACGATCGAGGTC
CTCACGCCAGATTTCCTGCGCAAGGACGGCGCGCTGGAGCAGATCGTCGCGGCCAAGCCCGATGTCTTCAACCACAATCT
CGAGACCGTGCCGTCGCGCTATCTCTCGGTCCGACCCGGCGCGCGCTACTTCCACTCGATCCGGCTGCTGCAGCGGGTCA
AGGAGATCGACCCGACCATCTTCACGAAGTCCGGCATCATGGTCGGCCTCGGCGAGCAGCGCCACGAGGTGTTGCAGGTG
ATGGATGACATGCGCTCGGCCGAGGTCGACTTTCTCACGATCGGGCAGTATCTGCAGCCCACCAAGAAGCACCACGCCGT
GATGAACTACGTCACTCCGGAGGAGTTCTCCAACTACGAGACCGTCGCCTACACCAAGGGCTTCCTGATGGTGTCGGCGA
GCCCGCTCACGCGCTCCTCGCACCATGCCGGCGAGGACTTCGCCAAGCTGAAGGCCGCGCGCGACGCGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
GCTGCCCATATCTGCCATGCCCTGGTATCCTCGGCCAGCCGGTTGCGGCGCGCCGCGGCTCGGAATAGCTAGGGCGCAAC
AGAGCTTTGCTTGGGTTCCC

Downstream 100 bases:

>100_bases
TCGAGACCAGATGCCGAGATTTTCCAACAAGCGGCGCGTGCCGCATACGGCCGACCAGATGTTCGACCTCGTCGCCGATG
TCGAACGCTATCCGGAATTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVTIVDINANTPLRPRHPEKVNRPDSVSPAKPAWIRVKAPTTRGYADTRNIVRENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPAALDAGEPEHVAEATFKLGLAHVVVTSVDRDDLADGGAAHIAETIRAIRAKCPTTTIEV
LTPDFLRKDGALEQIVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKEIDPTIFTKSGIMVGLGEQRHEVLQV
MDDMRSAEVDFLTIGQYLQPTKKHHAVMNYVTPEEFSNYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLKAARDALAR

Sequences:

>Translated_319_residues
MVTIVDINANTPLRPRHPEKVNRPDSVSPAKPAWIRVKAPTTRGYADTRNIVRENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPAALDAGEPEHVAEATFKLGLAHVVVTSVDRDDLADGGAAHIAETIRAIRAKCPTTTIEV
LTPDFLRKDGALEQIVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKEIDPTIFTKSGIMVGLGEQRHEVLQV
MDDMRSAEVDFLTIGQYLQPTKKHHAVMNYVTPEEFSNYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLKAARDALAR
>Mature_319_residues
MVTIVDINANTPLRPRHPEKVNRPDSVSPAKPAWIRVKAPTTRGYADTRNIVRENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPAALDAGEPEHVAEATFKLGLAHVVVTSVDRDDLADGGAAHIAETIRAIRAKCPTTTIEV
LTPDFLRKDGALEQIVAAKPDVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKEIDPTIFTKSGIMVGLGEQRHEVLQV
MDDMRSAEVDFLTIGQYLQPTKKHHAVMNYVTPEEFSNYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLKAARDALAR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=46.3157894736842, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=46.5020576131687, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=51.4492753623188, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=46.1240310077519, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=284, Percent_Identity=47.887323943662, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.2897526501767, Blast_Score=257, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35192; Mature: 35192

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIVDINANTPLRPRHPEKVNRPDSVSPAKPAWIRVKAPTTRGYADTRNIVRENGLVTV
CEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPAALDAGEPEHVAEATFKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAHVVVTSVDRDDLADGGAAHIAETIRAIRAKCPTTTIEVLTPDFLRKDGALEQIVAAKP
HHHHEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKEIDPTIFTKSGIMVGLGEQRHEVLQV
CHHCCCHHHHCHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHH
MDDMRSAEVDFLTIGQYLQPTKKHHAVMNYVTPEEFSNYETVAYTKGFLMVSASPLTRSS
HHHHHHCCCCEEECCHHHCHHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEECCCCCCCC
HHAGEDFAKLKAARDALAR
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTIVDINANTPLRPRHPEKVNRPDSVSPAKPAWIRVKAPTTRGYADTRNIVRENGLVTV
CEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPAALDAGEPEHVAEATFKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAHVVVTSVDRDDLADGGAAHIAETIRAIRAKCPTTTIEVLTPDFLRKDGALEQIVAAKP
HHHHEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKEIDPTIFTKSGIMVGLGEQRHEVLQV
CHHCCCHHHHCHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHH
MDDMRSAEVDFLTIGQYLQPTKKHHAVMNYVTPEEFSNYETVAYTKGFLMVSASPLTRSS
HHHHHHCCCCEEECCHHHCHHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEECCCCCCCC
HHAGEDFAKLKAARDALAR
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA