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The map label for this gene is nthB

Identifier: 146340788

GI number: 146340788

Start: 4019718

End: 4020377

Strand: Direct

Name: nthB

Synonym: BRADO3846

Alternate gene names: NA

Gene position: 4019718-4020377 (Clockwise)

Preceding gene: 146340787

Following gene: 146340789

Centisome position: 53.91

GC content: 68.79

Gene sequence:

>660_bases
ATGGACGGCGCACATGACATGGGCGGCGTTGCCGACTTCGGTCCGGTCCGCCCCGAACCCGACGAGCCGGTGTTTCACGC
CGAATGGGAGCGACGTGCGTTCGCGCTGACCTTGGCGATGGCCACGCCTGGCGGCTGGAACATCGACATGTCGCGCTTCG
CCCGCGAGAACCGCGCGCCGGCCGACTATCTGTCCAAGAGCTACTACCAGATCTGGCTCGCCGGCATCGAGCGCCTGACC
GCGGAACGGCATCTCGTCGAGGCGGACGAGATCGCCGCCGGCAGGATGCTGCATCCGGCGCGGCCGGTCGCGAAGATCCT
CAAAGCGGACGATGTCCCCGTCACGCTGGGCCGGGGCGGACCGACCGAGCGCGCGGCTACGACCTCGGCGCTGTTTGCCG
TTGGCGACTGCGTGCGCGCCAAGGACATGCATCCGCCGACGCATACGCGGCTGCCGCGCTACGTGCGCGGCCATGTCGGC
CGCATCGAGGCCGTCCGGGGCGTGCACGTGTTCCCGGACAGCAATGCCCATGGCCAAGGCGAAAACCCGCAATGGCTTTA
CACGGTGTCGTTCAGCGGCGCGGAGCTGTGGGCCGATGCACCCGATCCGCTGCTCGAGGTCACCGTCGACGCCTTCGAGC
CCTATCTGGAGCGCGCATGA

Upstream 100 bases:

>100_bases
GCACCGAGGGCTGGAGCGAAGATCGGCTCGCCGAGCTCGTCACGCGCGACAGCATGATCGGGACCGGCCTGCCCAGGCGT
GCTGACGAGGTGACCCGCTG

Downstream 100 bases:

>100_bases
GCGCGCCCGATCATCGGGCCGCGCTTGCGGCGACGCGTGCCCTGCCCGGCATTCCCGTCGATGCCGAAGGGCCGGTGTTT
CGTGAACCGTGGGAGGCGCA

Product: nitrile hydratase subunit beta

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDGAHDMGGVADFGPVRPEPDEPVFHAEWERRAFALTLAMATPGGWNIDMSRFARENRAPADYLSKSYYQIWLAGIERLT
AERHLVEADEIAAGRMLHPARPVAKILKADDVPVTLGRGGPTERAATTSALFAVGDCVRAKDMHPPTHTRLPRYVRGHVG
RIEAVRGVHVFPDSNAHGQGENPQWLYTVSFSGAELWADAPDPLLEVTVDAFEPYLERA

Sequences:

>Translated_219_residues
MDGAHDMGGVADFGPVRPEPDEPVFHAEWERRAFALTLAMATPGGWNIDMSRFARENRAPADYLSKSYYQIWLAGIERLT
AERHLVEADEIAAGRMLHPARPVAKILKADDVPVTLGRGGPTERAATTSALFAVGDCVRAKDMHPPTHTRLPRYVRGHVG
RIEAVRGVHVFPDSNAHGQGENPQWLYTVSFSGAELWADAPDPLLEVTVDAFEPYLERA
>Mature_219_residues
MDGAHDMGGVADFGPVRPEPDEPVFHAEWERRAFALTLAMATPGGWNIDMSRFARENRAPADYLSKSYYQIWLAGIERLT
AERHLVEADEIAAGRMLHPARPVAKILKADDVPVTLGRGGPTERAATTSALFAVGDCVRAKDMHPPTHTRLPRYVRGHVG
RIEAVRGVHVFPDSNAHGQGENPQWLYTVSFSGAELWADAPDPLLEVTVDAFEPYLERA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 24147; Mature: 24147

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGAHDMGGVADFGPVRPEPDEPVFHAEWERRAFALTLAMATPGGWNIDMSRFARENRAP
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCC
ADYLSKSYYQIWLAGIERLTAERHLVEADEIAAGRMLHPARPVAKILKADDVPVTLGRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHCCCCCCEEECCCC
PTERAATTSALFAVGDCVRAKDMHPPTHTRLPRYVRGHVGRIEAVRGVHVFPDSNAHGQG
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHCCCCEEEECCEEECCCCCCCCCC
ENPQWLYTVSFSGAELWADAPDPLLEVTVDAFEPYLERA
CCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDGAHDMGGVADFGPVRPEPDEPVFHAEWERRAFALTLAMATPGGWNIDMSRFARENRAP
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCC
ADYLSKSYYQIWLAGIERLTAERHLVEADEIAAGRMLHPARPVAKILKADDVPVTLGRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHCCCCCCEEECCCC
PTERAATTSALFAVGDCVRAKDMHPPTHTRLPRYVRGHVGRIEAVRGVHVFPDSNAHGQG
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHCCCCEEEECCEEECCCCCCCCCC
ENPQWLYTVSFSGAELWADAPDPLLEVTVDAFEPYLERA
CCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]