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The map label for this gene is nthA [H]

Identifier: 146340787

GI number: 146340787

Start: 4019083

End: 4019718

Strand: Direct

Name: nthA [H]

Synonym: BRADO3845

Alternate gene names: 146340787

Gene position: 4019083-4019718 (Clockwise)

Preceding gene: 146340784

Following gene: 146340788

Centisome position: 53.9

GC content: 66.51

Gene sequence:

>636_bases
ATGAGCCATCACGATCATCATCACGACCATCACCACGATCATTCGGACCTGTCCGAGACCGAGCTGCGCGTCCGCGCGCT
CGAAACGATCCTCACCGAGAAGGGCTACATCGATCCGGCTGCCCTCGACGTGCTTATCGAGACCTATGAAAGCAAGGTCG
GACCGCGCAATGGGGCCCGGGTGGTGGCCCGCGCCTGGAGCGATCCGGCGTTTCGATCGCGGCTGTTGTCCGATGCGACG
CCCGCGATCGGCGAGCTCGGCTATGCCGGCCGCCAGGGCGAGCACATCGTCGCGGTCGAGAACACGCCGGAGCTGCACAA
CATGGTCGTGTGCACCCTGTGCTCCTGTTACCCCTGGCCGGTGCTCGGCTTGCCGCCGGTCTGGTACAAATCGGCGCCCT
ATCGCGCCAAGGCCGTGAAGGACCCGCGCGGCGTGCTGCGCGATTTCGGCTTCGAGCTGCCGCCGACCACGAAGATCAGG
GTCTGGGATTCGACCGCGGAAATCCGCTACTTCGTCCTGCCGATGCGCCCCGAGGGCACCGAGGGCTGGAGCGAAGATCG
GCTCGCCGAGCTCGTCACGCGCGACAGCATGATCGGGACCGGCCTGCCCAGGCGTGCTGACGAGGTGACCCGCTGA

Upstream 100 bases:

>100_bases
CGCCTGGACGAAGTTGCATGCCCAGCGTGTTCTCGCGCCGCACAACCACCGGAGACGGCCATCGCTCGACAGCGCGAGGC
GATCCCCCTATTTTCGCACC

Downstream 100 bases:

>100_bases
TGGACGGCGCACATGACATGGGCGGCGTTGCCGACTTCGGTCCGGTCCGCCCCGAACCCGACGAGCCGGTGTTTCACGCC
GAATGGGAGCGACGTGCGTT

Product: nitrile hydratase subunit alpha

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSHHDHHHDHHHDHSDLSETELRVRALETILTEKGYIDPAALDVLIETYESKVGPRNGARVVARAWSDPAFRSRLLSDAT
PAIGELGYAGRQGEHIVAVENTPELHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRAKAVKDPRGVLRDFGFELPPTTKIR
VWDSTAEIRYFVLPMRPEGTEGWSEDRLAELVTRDSMIGTGLPRRADEVTR

Sequences:

>Translated_211_residues
MSHHDHHHDHHHDHSDLSETELRVRALETILTEKGYIDPAALDVLIETYESKVGPRNGARVVARAWSDPAFRSRLLSDAT
PAIGELGYAGRQGEHIVAVENTPELHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRAKAVKDPRGVLRDFGFELPPTTKIR
VWDSTAEIRYFVLPMRPEGTEGWSEDRLAELVTRDSMIGTGLPRRADEVTR
>Mature_210_residues
SHHDHHHDHHHDHSDLSETELRVRALETILTEKGYIDPAALDVLIETYESKVGPRNGARVVARAWSDPAFRSRLLSDATP
AIGELGYAGRQGEHIVAVENTPELHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRAKAVKDPRGVLRDFGFELPPTTKIRV
WDSTAEIRYFVLPMRPEGTEGWSEDRLAELVTRDSMIGTGLPRRADEVTR

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23798; Mature: 23667

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHDHHHDHHHDHSDLSETELRVRALETILTEKGYIDPAALDVLIETYESKVGPRNGAR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCE
VVARAWSDPAFRSRLLSDATPAIGELGYAGRQGEHIVAVENTPELHNMVVCTLCSCYPWP
EEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
VLGLPPVWYKSAPYRAKAVKDPRGVLRDFGFELPPTTKIRVWDSTAEIRYFVLPMRPEGT
CCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCCCC
EGWSEDRLAELVTRDSMIGTGLPRRADEVTR
CCCCHHHHHHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
SHHDHHHDHHHDHSDLSETELRVRALETILTEKGYIDPAALDVLIETYESKVGPRNGAR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCE
VVARAWSDPAFRSRLLSDATPAIGELGYAGRQGEHIVAVENTPELHNMVVCTLCSCYPWP
EEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
VLGLPPVWYKSAPYRAKAVKDPRGVLRDFGFELPPTTKIRVWDSTAEIRYFVLPMRPEGT
CCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCCCC
EGWSEDRLAELVTRDSMIGTGLPRRADEVTR
CCCCHHHHHHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]