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The map label for this gene is ycfH [C]

Identifier: 146340772

GI number: 146340772

Start: 4001533

End: 4002324

Strand: Direct

Name: ycfH [C]

Synonym: BRADO3830

Alternate gene names: 146340772

Gene position: 4001533-4002324 (Clockwise)

Preceding gene: 146340771

Following gene: 146340773

Centisome position: 53.66

GC content: 64.9

Gene sequence:

>792_bases
ATGCTGGTCGACTCTCATTGCCATCTGGATTTTCCGGATTTTGCCGAGGATCTGGATGGCATCGTCACGCGCGCCGCCGC
TGCCGGCGTCGGCCGCCTGGTGACGATCTCGACCCGGGTGCGCAGGCTACCCGAGTTGCTTGCGATCGCAGAACGGTTCG
AGAATGTCTACTGCTCGGTCGGCACGCATCCTCACAATGCAGATGAGGAGGACGGCATTTCGGCCGAGGAGCTGATCGCG
CTGACCAGGCATCCCAAGGTCGTCGCACTCGGCGAGGCGGGGCTCGACAATTTCTACGACCACGGCTCCAAGGACGCGCA
GGAGCGCGGGTTCCGCGCCCATATCGCCGCGGCCCGGGAAACCGGCCTGCCGCTGGTGATCCACACCCGCGAGGCCGACG
AGCAGTGCAGCCGCATCCTCGACGAGGAAATGGCGCGCGGACCTTTCAAGGCCGTGCTGCATTGCTACACCGGCGGACGC
AAGCTGGCGATGAAGGCAATCGATCTCGGACTGCACATCTCGTTCACGGGCATCGTGACGTTCAAGAAGTCGGAGACGCT
GCGCGCGCTCGCCGCCGAGGTGCCGGCCGATCGCATCATGGTGGAGACCGACGCGCCGTATCTGGCACCCGGCAAATTCC
GCGGCAAGCGCAACGAGCCGGCCTATGTGGTGGAGACGGCGAAGGTGCTGGCGGAAACCCGCGGCGTGTCGTTCGAGGAA
TTCTCGCGCCAGACCACCGAGACGTTCTTCAAGCTGTTCGCCAAGGTGCCGCGACCCGAGGTGCTGGCATGA

Upstream 100 bases:

>100_bases
GCTTCGCGCGGCTCGGCGGCGAGGTGCGGATCGCACCGGGCACGCAGCTGCCAGCGCCGCAGGCGGTGTTCCCGCGCTAC
ATCGAGCCGAGCGCGGCGTG

Downstream 100 bases:

>100_bases
CCCTGACGCTCACGATCCTCGGCTGCGGCTCCTCGGCCGGCGTGCCGCGACCGGCGCTGGGGTGGGGCGCCTGCGATCCG
AACAACCCCAGGAATCGTCG

Product: putative deoxyribonuclease (ycfH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLVDSHCHLDFPDFAEDLDGIVTRAAAAGVGRLVTISTRVRRLPELLAIAERFENVYCSVGTHPHNADEEDGISAEELIA
LTRHPKVVALGEAGLDNFYDHGSKDAQERGFRAHIAAARETGLPLVIHTREADEQCSRILDEEMARGPFKAVLHCYTGGR
KLAMKAIDLGLHISFTGIVTFKKSETLRALAAEVPADRIMVETDAPYLAPGKFRGKRNEPAYVVETAKVLAETRGVSFEE
FSRQTTETFFKLFAKVPRPEVLA

Sequences:

>Translated_263_residues
MLVDSHCHLDFPDFAEDLDGIVTRAAAAGVGRLVTISTRVRRLPELLAIAERFENVYCSVGTHPHNADEEDGISAEELIA
LTRHPKVVALGEAGLDNFYDHGSKDAQERGFRAHIAAARETGLPLVIHTREADEQCSRILDEEMARGPFKAVLHCYTGGR
KLAMKAIDLGLHISFTGIVTFKKSETLRALAAEVPADRIMVETDAPYLAPGKFRGKRNEPAYVVETAKVLAETRGVSFEE
FSRQTTETFFKLFAKVPRPEVLA
>Mature_263_residues
MLVDSHCHLDFPDFAEDLDGIVTRAAAAGVGRLVTISTRVRRLPELLAIAERFENVYCSVGTHPHNADEEDGISAEELIA
LTRHPKVVALGEAGLDNFYDHGSKDAQERGFRAHIAAARETGLPLVIHTREADEQCSRILDEEMARGPFKAVLHCYTGGR
KLAMKAIDLGLHISFTGIVTFKKSETLRALAAEVPADRIMVETDAPYLAPGKFRGKRNEPAYVVETAKVLAETRGVSFEE
FSRQTTETFFKLFAKVPRPEVLA

Specific function: Unknown

COG id: COG0084

COG function: function code L; Mg-dependent DNase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tatD DNase family [H]

Homologues:

Organism=Homo sapiens, GI110349730, Length=268, Percent_Identity=30.2238805970149, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI110349734, Length=268, Percent_Identity=29.8507462686567, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI226061614, Length=257, Percent_Identity=30.3501945525292, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI226061853, Length=276, Percent_Identity=29.7101449275362, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI225903424, Length=223, Percent_Identity=29.1479820627803, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI14042943, Length=267, Percent_Identity=25.0936329588015, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI226061595, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI225903439, Length=236, Percent_Identity=25.8474576271186, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787342, Length=256, Percent_Identity=42.1875, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI87082439, Length=254, Percent_Identity=31.1023622047244, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI48994985, Length=239, Percent_Identity=33.0543933054393, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559024, Length=284, Percent_Identity=29.9295774647887, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71980746, Length=265, Percent_Identity=27.1698113207547, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17565396, Length=296, Percent_Identity=27.3648648648649, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543026, Length=289, Percent_Identity=26.9896193771626, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648690, Length=283, Percent_Identity=28.2685512367491, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24586117, Length=211, Percent_Identity=32.2274881516588, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221330018, Length=211, Percent_Identity=32.2274881516588, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015992
- InterPro:   IPR001130
- InterPro:   IPR018228
- InterPro:   IPR012278
- InterPro:   IPR015991 [H]

Pfam domain/function: PF01026 TatD_DNase [H]

EC number: 3.1.21.-

Molecular weight: Translated: 29000; Mature: 29000

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01137 TATD_1 ; PS01090 TATD_2 ; PS01091 TATD_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVDSHCHLDFPDFAEDLDGIVTRAAAAGVGRLVTISTRVRRLPELLAIAERFENVYCSV
CEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEC
GTHPHNADEEDGISAEELIALTRHPKVVALGEAGLDNFYDHGSKDAQERGFRAHIAAARE
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHCCCHHHHHHHHH
TGLPLVIHTREADEQCSRILDEEMARGPFKAVLHCYTGGRKLAMKAIDLGLHISFTGIVT
CCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEHHHHHCCEEEEEEEEEE
FKKSETLRALAAEVPADRIMVETDAPYLAPGKFRGKRNEPAYVVETAKVLAETRGVSFEE
ECCCHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
FSRQTTETFFKLFAKVPRPEVLA
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLVDSHCHLDFPDFAEDLDGIVTRAAAAGVGRLVTISTRVRRLPELLAIAERFENVYCSV
CEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEC
GTHPHNADEEDGISAEELIALTRHPKVVALGEAGLDNFYDHGSKDAQERGFRAHIAAARE
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCHHHCCCHHHHHHHHH
TGLPLVIHTREADEQCSRILDEEMARGPFKAVLHCYTGGRKLAMKAIDLGLHISFTGIVT
CCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEHHHHHCCEEEEEEEEEE
FKKSETLRALAAEVPADRIMVETDAPYLAPGKFRGKRNEPAYVVETAKVLAETRGVSFEE
ECCCHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
FSRQTTETFFKLFAKVPRPEVLA
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]