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The map label for this gene is cysJ [H]

Identifier: 146340740

GI number: 146340740

Start: 3966073

End: 3967680

Strand: Direct

Name: cysJ [H]

Synonym: BRADO3794

Alternate gene names: 146340740

Gene position: 3966073-3967680 (Clockwise)

Preceding gene: 146340739

Following gene: 146340744

Centisome position: 53.19

GC content: 66.98

Gene sequence:

>1608_bases
ATGAACCAGATCACACCGATGCCGAAGCTCGAGATCATTCCTCCGACCGCGCCGTTCTCGGAAGCGCAGCGCTCCTGGCT
CAACGGCTTCCTCGCCGGCGCGCTCGGCCTCGACGGCTCGACGCCATTGGCTCCTGCGCAGAACGGCACCGCGCTTGCTC
CCGCCGGCGACGGCGATGACGGCGAGGCGCCGTGGCACGATCAGACCATGCCAATCACCGAGCGCATGAAGCTTGCCGAA
GGCCGCCCGCTGCGCCGTCGTATGATGGCGGCGATGGCGCAGCAGGACTGCGGCCAGTGCGGCTACAATTGCAGCGACTA
CTCGGACGCGATCGCCGATCACAGCGAGACGCGCCTCAATCTGTGCGTCCCCGGCGGCAAGGAAACCGCGCGGATGCTGA
AGGCGCTGTATGAGGAGATCGACAAGGCTTCGCCTGCCGCCAAGCCGGCGGCCGTGACCGCGACGCCGGCCAACGTCTCC
GTTGTCGTCAGCGCGCCCGGCCGCTCGCGCGACAATCCGGTCGAGGCGACGTTCCTGTCGCGGCGGCTGCTGAACAAGCC
GGGCTCGGAGAAGGAGACCTGGCACATCGAGTTCGATCTCGCGGAGGCCGGGCTCGACTACGTCGTCGGCGATTCCTTCG
GCATCTTTGCCAAGAACGATCTCGGCCTCGTCGACCAGATCATCGCGTTGCTCGGCGCCTCGCACATGACCGAGGTCAAC
GGCAAGCCGCTGCGGCAGTGCCTGCTCGAGGACGTCTCGCTGTCACCGGCGCCGGATTCGCTATTCGAGCTGATCTCCTT
CATCACCGGCGGCGCCACGCGCGAGAAGGCGCGGCGGCTGGCGCAGGGCGAGGATCCCGATGGCGATGCCGCGACGCTCG
ACGTCATGGCTGTGCTGCAGAAGTTCTCCGGCGTCCGGCCGCATCCGGAAGCGTTCGTCGAGGCGCTGGAGCCGCTGCAG
CCGCGGCTGTACTCGATCTCCTCGTCGCACAACGCCACGCCCGGCAAACTCTCGCTCACCGTCGACTGCGTCCGCTACGT
CATCGGCAAGCGCAAGCGGCTTGGCCTTGCCTCGACGTTCCTCGCCGAGCGTATCAATCCCGGCGACAGACTGCGCGTCT
ACGTGCAGAAGGCGCACGGCTTTGCACTGCCGGCCGATCCGAAGACGCCGATCATCATGGTCGGCCCGGGCACCGGCATC
GCGCCGTTCCGCGCCTTCCTGCATGATCGCAGGGCGACCGGTGCGCCGGGCAAGAACTGGCTGTTCTTCGGCCACCAGCG
CAGCGACTGCGACTTCTTCTACGCCGATGAGCTCAATGCGATGAAGACCTCTGGCCTGTTGACGCGGCTGTCGCTGGCGT
GGTCGCGCGACGGCGACAAGAAGTTCTACGTCCAGGACCGCATGCGCGAGGTCGGTCGCGAGGTGTGGACCTGGCTCGCC
GGCGGCGCGCATCTCTACATCTGCGGCGACGCTAAGCGCATGGCCAAGGATGTCGAGCGCGCGCTCGTCGACATCGTCGC
CCAGTTCGGCGCCCGCTCGACCGACGAGGCCGTCAGCTTCGTCGCGGACCTCAAGAAGAAAGGCCGGTTCCAGCTGGACG
TTTATTAA

Upstream 100 bases:

>100_bases
GCACTTCGCCGGAAGAGACCTTCTTGTCGTTTGCCCGCCGCCATGACGGCGACGCTCTTCGCAAGCTCGCCGAAGCCCAG
ACCTCGTCCGAGGTGTCAGC

Downstream 100 bases:

>100_bases
CCCGCCGGAACCTCAAGAAAGGCGGCGGATAAAATTCTCGGCCGGAACTCCGGCCGAGAATGTTTTGGTTCCCTGCAGAT
CCGAAACAGGAGTCTTGTCG

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNQITPMPKLEIIPPTAPFSEAQRSWLNGFLAGALGLDGSTPLAPAQNGTALAPAGDGDDGEAPWHDQTMPITERMKLAE
GRPLRRRMMAAMAQQDCGQCGYNCSDYSDAIADHSETRLNLCVPGGKETARMLKALYEEIDKASPAAKPAAVTATPANVS
VVVSAPGRSRDNPVEATFLSRRLLNKPGSEKETWHIEFDLAEAGLDYVVGDSFGIFAKNDLGLVDQIIALLGASHMTEVN
GKPLRQCLLEDVSLSPAPDSLFELISFITGGATREKARRLAQGEDPDGDAATLDVMAVLQKFSGVRPHPEAFVEALEPLQ
PRLYSISSSHNATPGKLSLTVDCVRYVIGKRKRLGLASTFLAERINPGDRLRVYVQKAHGFALPADPKTPIIMVGPGTGI
APFRAFLHDRRATGAPGKNWLFFGHQRSDCDFFYADELNAMKTSGLLTRLSLAWSRDGDKKFYVQDRMREVGREVWTWLA
GGAHLYICGDAKRMAKDVERALVDIVAQFGARSTDEAVSFVADLKKKGRFQLDVY

Sequences:

>Translated_535_residues
MNQITPMPKLEIIPPTAPFSEAQRSWLNGFLAGALGLDGSTPLAPAQNGTALAPAGDGDDGEAPWHDQTMPITERMKLAE
GRPLRRRMMAAMAQQDCGQCGYNCSDYSDAIADHSETRLNLCVPGGKETARMLKALYEEIDKASPAAKPAAVTATPANVS
VVVSAPGRSRDNPVEATFLSRRLLNKPGSEKETWHIEFDLAEAGLDYVVGDSFGIFAKNDLGLVDQIIALLGASHMTEVN
GKPLRQCLLEDVSLSPAPDSLFELISFITGGATREKARRLAQGEDPDGDAATLDVMAVLQKFSGVRPHPEAFVEALEPLQ
PRLYSISSSHNATPGKLSLTVDCVRYVIGKRKRLGLASTFLAERINPGDRLRVYVQKAHGFALPADPKTPIIMVGPGTGI
APFRAFLHDRRATGAPGKNWLFFGHQRSDCDFFYADELNAMKTSGLLTRLSLAWSRDGDKKFYVQDRMREVGREVWTWLA
GGAHLYICGDAKRMAKDVERALVDIVAQFGARSTDEAVSFVADLKKKGRFQLDVY
>Mature_535_residues
MNQITPMPKLEIIPPTAPFSEAQRSWLNGFLAGALGLDGSTPLAPAQNGTALAPAGDGDDGEAPWHDQTMPITERMKLAE
GRPLRRRMMAAMAQQDCGQCGYNCSDYSDAIADHSETRLNLCVPGGKETARMLKALYEEIDKASPAAKPAAVTATPANVS
VVVSAPGRSRDNPVEATFLSRRLLNKPGSEKETWHIEFDLAEAGLDYVVGDSFGIFAKNDLGLVDQIIALLGASHMTEVN
GKPLRQCLLEDVSLSPAPDSLFELISFITGGATREKARRLAQGEDPDGDAATLDVMAVLQKFSGVRPHPEAFVEALEPLQ
PRLYSISSSHNATPGKLSLTVDCVRYVIGKRKRLGLASTFLAERINPGDRLRVYVQKAHGFALPADPKTPIIMVGPGTGI
APFRAFLHDRRATGAPGKNWLFFGHQRSDCDFFYADELNAMKTSGLLTRLSLAWSRDGDKKFYVQDRMREVGREVWTWLA
GGAHLYICGDAKRMAKDVERALVDIVAQFGARSTDEAVSFVADLKKKGRFQLDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=399, Percent_Identity=32.5814536340852, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI7657393, Length=408, Percent_Identity=30.3921568627451, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI24041029, Length=392, Percent_Identity=32.3979591836735, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI221316705, Length=407, Percent_Identity=29.7297297297297, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI221316709, Length=398, Percent_Identity=30.1507537688442, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI169790956, Length=410, Percent_Identity=29.7560975609756, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI169790958, Length=410, Percent_Identity=29.7560975609756, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI10835173, Length=405, Percent_Identity=30.3703703703704, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI40254422, Length=410, Percent_Identity=30.7317073170732, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI221316707, Length=355, Percent_Identity=29.5774647887324, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1789123, Length=416, Percent_Identity=40.8653846153846, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17554134, Length=402, Percent_Identity=30.8457711442786, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17566446, Length=285, Percent_Identity=30.5263157894737, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531441, Length=317, Percent_Identity=31.5457413249211, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321143, Length=377, Percent_Identity=29.7082228116711, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321832, Length=423, Percent_Identity=29.3144208037825, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325305, Length=270, Percent_Identity=27.4074074074074, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137192, Length=399, Percent_Identity=31.5789473684211, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24582192, Length=399, Percent_Identity=31.5789473684211, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI78706872, Length=380, Percent_Identity=31.3157894736842, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24583543, Length=380, Percent_Identity=31.3157894736842, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI78706876, Length=380, Percent_Identity=31.3157894736842, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24660907, Length=232, Percent_Identity=30.1724137931034, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24660903, Length=232, Percent_Identity=30.1724137931034, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 58273; Mature: 58273

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQITPMPKLEIIPPTAPFSEAQRSWLNGFLAGALGLDGSTPLAPAQNGTALAPAGDGDD
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCC
GEAPWHDQTMPITERMKLAEGRPLRRRMMAAMAQQDCGQCGYNCSDYSDAIADHSETRLN
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEE
LCVPGGKETARMLKALYEEIDKASPAAKPAAVTATPANVSVVVSAPGRSRDNPVEATFLS
EECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHH
RRLLNKPGSEKETWHIEFDLAEAGLDYVVGDSFGIFAKNDLGLVDQIIALLGASHMTEVN
HHHHCCCCCCCCEEEEEEEHHHCCCCEEECCCCCEEECCCCCHHHHHHHHHCCHHHHCCC
GKPLRQCLLEDVSLSPAPDSLFELISFITGGATREKARRLAQGEDPDGDAATLDVMAVLQ
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
KFSGVRPHPEAFVEALEPLQPRLYSISSSHNATPGKLSLTVDCVRYVIGKRKRLGLASTF
HHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCEEEEHHHHHHHHHCCCHHCCHHHHH
LAERINPGDRLRVYVQKAHGFALPADPKTPIIMVGPGTGIAPFRAFLHDRRATGAPGKNW
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
LFFGHQRSDCDFFYADELNAMKTSGLLTRLSLAWSRDGDKKFYVQDRMREVGREVWTWLA
EEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHC
GGAHLYICGDAKRMAKDVERALVDIVAQFGARSTDEAVSFVADLKKKGRFQLDVY
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MNQITPMPKLEIIPPTAPFSEAQRSWLNGFLAGALGLDGSTPLAPAQNGTALAPAGDGDD
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCC
GEAPWHDQTMPITERMKLAEGRPLRRRMMAAMAQQDCGQCGYNCSDYSDAIADHSETRLN
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEE
LCVPGGKETARMLKALYEEIDKASPAAKPAAVTATPANVSVVVSAPGRSRDNPVEATFLS
EECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHH
RRLLNKPGSEKETWHIEFDLAEAGLDYVVGDSFGIFAKNDLGLVDQIIALLGASHMTEVN
HHHHCCCCCCCCEEEEEEEHHHCCCCEEECCCCCEEECCCCCHHHHHHHHHCCHHHHCCC
GKPLRQCLLEDVSLSPAPDSLFELISFITGGATREKARRLAQGEDPDGDAATLDVMAVLQ
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
KFSGVRPHPEAFVEALEPLQPRLYSISSSHNATPGKLSLTVDCVRYVIGKRKRLGLASTF
HHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCEEEEHHHHHHHHHCCCHHCCHHHHH
LAERINPGDRLRVYVQKAHGFALPADPKTPIIMVGPGTGIAPFRAFLHDRRATGAPGKNW
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCE
LFFGHQRSDCDFFYADELNAMKTSGLLTRLSLAWSRDGDKKFYVQDRMREVGREVWTWLA
EEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHC
GGAHLYICGDAKRMAKDVERALVDIVAQFGARSTDEAVSFVADLKKKGRFQLDVY
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]