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The map label for this gene is rhtB [H]

Identifier: 146340712

GI number: 146340712

Start: 3930631

End: 3931245

Strand: Direct

Name: rhtB [H]

Synonym: BRADO3764

Alternate gene names: 146340712

Gene position: 3930631-3931245 (Clockwise)

Preceding gene: 146340711

Following gene: 146340713

Centisome position: 52.71

GC content: 66.83

Gene sequence:

>615_bases
TTGGCTCTCGACACTTGGTTTCTGTTCCTGCTGACGAGCATCGGGATCTCGATCACGCCCGGGCCCAATGGCCTGCTGGC
CCTGACACACGGCGCGCTGCATGGCGGGCGGAAGACGCTGTTCACGATCGCCGGCGGCCTGATCGGCTTCGTCCTGATCA
TGGCGCTGTGCATGTTCGGGATCGGCGCGCTGGTGCAGTCCTCGATCACCTGGCTCTCCGTGCTGAAGTGGATCGGCGGG
CTTTATCTCGCCTGGCTCGGCGTCAAGCTGTGGCGCTCGCCACCGGTCGCCGTCGATGTCAGCGACAAGCCGGTCACGGC
GCGCGGGCGTGTGCTGTTTCGCCAGGGTTTCCTGACGGCCGCGACCAACCCGAAATGCCTGCTGTTCTTCAGCGCGCTAT
TGCCGCAATTCATCGATCCCGCGCGTAGCCTCGCCGTGCAGTTCGCGATCGTCGCCACCACCTATACGGTCACGGAGTTC
GTGACCGAGCTCGCCATCGCCAGCACCGCCGCCCGGGTGCGTCCCTGGCTGGCGCGGACCGGGCGGCGCTTCAACCAGGT
CTGCGGCGGCATCTTCGTGGCTGTGGGCCTCGCCTTGCCGTTGCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGAGCTCCCCCCACTTGCCATCCGATCCAAGATCCGACATCAGCGTCTGCGATGACTGGGCAGCGCATCCGTCGGTCCGC
TTGATCAAAGGGAAACCCGA

Downstream 100 bases:

>100_bases
CGGCTGCTTTTGCACGCCCTGCGCTGGCAGGGCCGCGGGACCAGCCCGCCACAGCGGGCCGGCAAAGCGCGGATGTGTCG
GAAAGAGGGCCTTGCCCTTC

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MALDTWFLFLLTSIGISITPGPNGLLALTHGALHGGRKTLFTIAGGLIGFVLIMALCMFGIGALVQSSITWLSVLKWIGG
LYLAWLGVKLWRSPPVAVDVSDKPVTARGRVLFRQGFLTAATNPKCLLFFSALLPQFIDPARSLAVQFAIVATTYTVTEF
VTELAIASTAARVRPWLARTGRRFNQVCGGIFVAVGLALPLRSA

Sequences:

>Translated_204_residues
MALDTWFLFLLTSIGISITPGPNGLLALTHGALHGGRKTLFTIAGGLIGFVLIMALCMFGIGALVQSSITWLSVLKWIGG
LYLAWLGVKLWRSPPVAVDVSDKPVTARGRVLFRQGFLTAATNPKCLLFFSALLPQFIDPARSLAVQFAIVATTYTVTEF
VTELAIASTAARVRPWLARTGRRFNQVCGGIFVAVGLALPLRSA
>Mature_203_residues
ALDTWFLFLLTSIGISITPGPNGLLALTHGALHGGRKTLFTIAGGLIGFVLIMALCMFGIGALVQSSITWLSVLKWIGGL
YLAWLGVKLWRSPPVAVDVSDKPVTARGRVLFRQGFLTAATNPKCLLFFSALLPQFIDPARSLAVQFAIVATTYTVTEFV
TELAIASTAARVRPWLARTGRRFNQVCGGIFVAVGLALPLRSA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=33.8383838383838, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1788099, Length=198, Percent_Identity=26.2626262626263, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21875; Mature: 21744

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDTWFLFLLTSIGISITPGPNGLLALTHGALHGGRKTLFTIAGGLIGFVLIMALCMFG
CCHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGALVQSSITWLSVLKWIGGLYLAWLGVKLWRSPPVAVDVSDKPVTARGRVLFRQGFLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCEEC
ATNPKCLLFFSALLPQFIDPARSLAVQFAIVATTYTVTEFVTELAIASTAARVRPWLART
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRFNQVCGGIFVAVGLALPLRSA
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALDTWFLFLLTSIGISITPGPNGLLALTHGALHGGRKTLFTIAGGLIGFVLIMALCMFG
CHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGALVQSSITWLSVLKWIGGLYLAWLGVKLWRSPPVAVDVSDKPVTARGRVLFRQGFLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCEEC
ATNPKCLLFFSALLPQFIDPARSLAVQFAIVATTYTVTEFVTELAIASTAARVRPWLART
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRFNQVCGGIFVAVGLALPLRSA
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]