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The map label for this gene is yjeA [H]

Identifier: 146340544

GI number: 146340544

Start: 3779338

End: 3780393

Strand: Direct

Name: yjeA [H]

Synonym: BRADO3587

Alternate gene names: 146340544

Gene position: 3779338-3780393 (Clockwise)

Preceding gene: 146340540

Following gene: 146340545

Centisome position: 50.68

GC content: 69.22

Gene sequence:

>1056_bases
ATGACAGGCCAGGAGCCATCCCCGTCGCCCTGGTGGTCGCCGCAGCGACATGCCGATGTCAGGCCATTCCTGCAGGCCCG
GGCCGCCATCACGCGCGCCCTTCGGGGGTGGTTCGAGGAGCAGGGGTTCACCGAGGTCGAAACCGGCATCCTCCAGCTGT
CCCCAGGCAACGAGACCCATCTGCACGCGCCAGCGACCGCGCTGATGGCGGCTGACGGGGTCCAACATAAAAGGTACTTG
AGAACCTCTCCGGAGTTTGCCTGCAAGAAGCTGCTGGCGGCGGGGGAGCGGCGGATCTTCGAATTTGCCCGGGTGTTCCG
CGACCGCGAGCGCGGCGACCTGCATCTGCCCGAATTTACGATGCTGGAATGGTACCGGGCCGAGGCGCCGTACGACGCCA
TCATGGCCGACACCGTGGTGGTGATCGCCCATGCCGCGCAGGCCACCGGGATCAGGCAGTTCGCCTTCCGCGGCCGCACC
GCCGATCCCTATGCCGAGCCCGAGCTGCTGACCGTGGCCGGCGCCTTCGAGCGTCATGCCGGCATCGACCTGCTGGCGAC
GATCCGCGACCGGACCGGCGACCGCGACGCGCTGGCGGCGGCCGCGCGGGGCAAGATCCGCATCGGCGCGGACGACACCT
GGTCGGACCTGTTCAGCAAGGTGCTGGTCGAGCACATCGAGCCGCAGCTGGGCAACAAGCGGGTGACGGTGCTGTATGAA
TATCCCTCGCCGGAGGCCGCGCTGGCGCGGGTCGCGGCGGATCCGCGCGTGGCCGAGCGGTTCGAGGTCTATGCCTGCGG
CGTCGAGCTCGCCAATGGCTTCGGCGAGCTGACGGACGCGCTCGAGCAGCGGCGGCGGTTCGAACTGGAGATGGCAGAGA
AGCAGCGGCGCTATGGCGAGCGCTATCCGCTCGATGAGGATTTCCTCGCCGCCGTGGCCGCGATGCCGCAGGCGAGCGGG
GTGGCGCTCGGCTTCGACCGGCTGGTGATGCTGGCGGCCGGCGCGGTCAGGATCGATCAGGTGGTTTGGACGCCGCCGGC
AGGTGAGCAGCGATGA

Upstream 100 bases:

>100_bases
CCCGGCAACGGGCAGAGCCGGGCGGCGGTCGGACGCCGGGTGCGGTTTCGGGGCGCAACATACTGATTTTGTCCGAGCTT
GCCAGTCTTTTGGCGACCGC

Downstream 100 bases:

>100_bases
ACAGGATCGAACGGCCGACGACGTTGCGCAGCGCGGCCGATCTCGTCGCACAAGGCCTGGCCGATCCGGGCGAGCAGGCG
ACGCTCGAACGCGTGGCGCA

Product: putative lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTGQEPSPSPWWSPQRHADVRPFLQARAAITRALRGWFEEQGFTEVETGILQLSPGNETHLHAPATALMAADGVQHKRYL
RTSPEFACKKLLAAGERRIFEFARVFRDRERGDLHLPEFTMLEWYRAEAPYDAIMADTVVVIAHAAQATGIRQFAFRGRT
ADPYAEPELLTVAGAFERHAGIDLLATIRDRTGDRDALAAAARGKIRIGADDTWSDLFSKVLVEHIEPQLGNKRVTVLYE
YPSPEAALARVAADPRVAERFEVYACGVELANGFGELTDALEQRRRFELEMAEKQRRYGERYPLDEDFLAAVAAMPQASG
VALGFDRLVMLAAGAVRIDQVVWTPPAGEQR

Sequences:

>Translated_351_residues
MTGQEPSPSPWWSPQRHADVRPFLQARAAITRALRGWFEEQGFTEVETGILQLSPGNETHLHAPATALMAADGVQHKRYL
RTSPEFACKKLLAAGERRIFEFARVFRDRERGDLHLPEFTMLEWYRAEAPYDAIMADTVVVIAHAAQATGIRQFAFRGRT
ADPYAEPELLTVAGAFERHAGIDLLATIRDRTGDRDALAAAARGKIRIGADDTWSDLFSKVLVEHIEPQLGNKRVTVLYE
YPSPEAALARVAADPRVAERFEVYACGVELANGFGELTDALEQRRRFELEMAEKQRRYGERYPLDEDFLAAVAAMPQASG
VALGFDRLVMLAAGAVRIDQVVWTPPAGEQR
>Mature_350_residues
TGQEPSPSPWWSPQRHADVRPFLQARAAITRALRGWFEEQGFTEVETGILQLSPGNETHLHAPATALMAADGVQHKRYLR
TSPEFACKKLLAAGERRIFEFARVFRDRERGDLHLPEFTMLEWYRAEAPYDAIMADTVVVIAHAAQATGIRQFAFRGRTA
DPYAEPELLTVAGAFERHAGIDLLATIRDRTGDRDALAAAARGKIRIGADDTWSDLFSKVLVEHIEPQLGNKRVTVLYEY
PSPEAALARVAADPRVAERFEVYACGVELANGFGELTDALEQRRRFELEMAEKQRRYGERYPLDEDFLAAVAAMPQASGV
ALGFDRLVMLAAGAVRIDQVVWTPPAGEQR

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=353, Percent_Identity=26.628895184136, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI5031815, Length=353, Percent_Identity=26.628895184136, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI87082379, Length=339, Percent_Identity=35.9882005899705, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1789256, Length=347, Percent_Identity=29.971181556196, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790571, Length=355, Percent_Identity=30.1408450704225, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=27.1386430678466, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=27.1386430678466, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=27.1386430678466, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320242, Length=351, Percent_Identity=27.0655270655271, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324256, Length=350, Percent_Identity=26, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640851, Length=343, Percent_Identity=26.8221574344023, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24640849, Length=343, Percent_Identity=26.8221574344023, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39160; Mature: 39029

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQEPSPSPWWSPQRHADVRPFLQARAAITRALRGWFEEQGFTEVETGILQLSPGNETH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCE
LHAPATALMAADGVQHKRYLRTSPEFACKKLLAAGERRIFEFARVFRDRERGDLHLPEFT
EECCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
MLEWYRAEAPYDAIMADTVVVIAHAAQATGIRQFAFRGRTADPYAEPELLTVAGAFERHA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHC
GIDLLATIRDRTGDRDALAAAARGKIRIGADDTWSDLFSKVLVEHIEPQLGNKRVTVLYE
CCHHEEHHHHCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
YPSPEAALARVAADPRVAERFEVYACGVELANGFGELTDALEQRRRFELEMAEKQRRYGE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYPLDEDFLAAVAAMPQASGVALGFDRLVMLAAGAVRIDQVVWTPPAGEQR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECEEEECCCCCCCC
>Mature Secondary Structure 
TGQEPSPSPWWSPQRHADVRPFLQARAAITRALRGWFEEQGFTEVETGILQLSPGNETH
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCE
LHAPATALMAADGVQHKRYLRTSPEFACKKLLAAGERRIFEFARVFRDRERGDLHLPEFT
EECCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
MLEWYRAEAPYDAIMADTVVVIAHAAQATGIRQFAFRGRTADPYAEPELLTVAGAFERHA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHC
GIDLLATIRDRTGDRDALAAAARGKIRIGADDTWSDLFSKVLVEHIEPQLGNKRVTVLYE
CCHHEEHHHHCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
YPSPEAALARVAADPRVAERFEVYACGVELANGFGELTDALEQRRRFELEMAEKQRRYGE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYPLDEDFLAAVAAMPQASGVALGFDRLVMLAAGAVRIDQVVWTPPAGEQR
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA