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The map label for this gene is purF [H]

Identifier: 146340259

GI number: 146340259

Start: 3478614

End: 3480146

Strand: Reverse

Name: purF [H]

Synonym: BRADO3280

Alternate gene names: 146340259

Gene position: 3480146-3478614 (Counterclockwise)

Preceding gene: 146340260

Following gene: 146340258

Centisome position: 46.67

GC content: 67.25

Gene sequence:

>1533_bases
ATGGACCAGCACCACGATTCTTCCGGCGAAGCTCCCATGGAAATCCAATCGGATCTGGACTTCGGACCTCAAGCCTACAC
GAGAGACCCTGACCTGGACGGAGACACGCTGCGCGAGGAGTGCGGCGTGTTCGGCATTTATGGGCATAGCGATGCCGCCG
CGATCACCGCGCTCGGCCTGCATGCGCTCCAGCATCGCGGCCAGGAAGCCGCCGGCATCGTCTGCTATGACGGCACCCGC
TTCCACAGCGAGCGGCGCCTCGGCCTGGTCGGCGACACCTTCTCCCGCCGCGAGGTGATCGAGCGCCTGCCCGGCACCGC
CGCGGTCGGCCATGTCCGCTACGCGACGACCGGCGCAACCATCCTGCGCAACGTGCAGCCGCTGTTCGCCGAGCTCAATG
CCGGCGGCTTCGCGGTCGGCCACAACGGCAATCTCACCAACGGTCTCACGCTCCGTCGCGAACTGGTGCGCGCCGGCGCG
ATCATGCAGTCGACCACCGACACCGAGGTGATCCTGCATCTCGTCGCGCATTCCCGCCGCAGCAACTTCATCGACCGCTT
CATCGAGGCGCTGCGCGCGCTGGAGGGCGCCTATTCGCTGGTCTGCCTTACCAACAAGAAGCTGATCGGCGCCCGCGATC
CCCTGGGCATCCGCCCGCTGGTCTATGGCGAGCTCGACGGCTGCCCGATCCTGGCGTCGGAGACCTGCGCGCTCGACATG
ATCGGCGCGCGCTACATCCGCGACATCGAGCCCGGCGAGGTCGTGATCTTCGACGAAGCCGGCATGCACAGCCACAAGCC
GTTCCCGCCGAAGCCCGCGCGTCCCTGCGTGTTCGAATACATCTACTTTGCCCGGCCCGATTCCGTGGTCGGCGGCCGCT
CGGTCTACGAGGTACGCAAGGCGTTCGGCGCTCAACTCGCGCGCGAGAGCCATCCCGAGGTGGATGTCGTCGTGCCGGTG
CCGGATTCCGGTGTGCCGGCGGCGATCGGCTACAGCCAGTATTCGGGCGTGCCGTTCGAGCTTGGCATCATCCGCAACCA
CTATGTCGGCCGTACCTTCATCCAGCCGACCCAGGCGATCCGCGAATCCGGCGTGCGCATGAAGCACTCGGCGAACCGCG
CCGCGATCGAAGGCAAGCGCATCATCCTGATCGACGATTCGCTGGTGCGCGGTACCACCTCGCGCAAGATCGTGCGCATG
ATGCGGGACGCTGGCGCCACCGAGGTGCACTTCCGTCTTGCGTCGCCGCCGATCCTCTACCCCGACTATTACGGCATCGA
CCTGCCGGACCGCGGCGGCCTGCTCGCGGCGACGCATTCGCTCGAGGAGATGCGCGAGCTGATCGGGGCGGATTCGCTGG
CGTTCCTGTCGATCGACGGCATGTACCGCGCGATGGGCGAGCCGGCCCGCGACCCCGCCGCACCGAAATACGCCGACCAC
TGCTTCACCGGCAGCTACCCCACCAGCCTCACCGACCAGTCGCTGGTCGAGCCGACCCAGCAGCAGCTGTCGCTGCTGGC
GGAAGCGAGCTGA

Upstream 100 bases:

>100_bases
CAGCGGGCGGCGATGGGTACAGCAAGCCTAACCGCGACAGCTTGAAGAAGCTGATCGAGAAACCCGCGGCCAAGTGAGCC
AACCCCAGAAGAGAGGCGCG

Downstream 100 bases:

>100_bases
GGCGGCCCCTCCTCACGCCGTCATTCCGGGGCGAGGCCACAGGCCTCGAACCCGGAACCCCGAAGTTTCATTCTGGAATC
ATGAAGGTCCGAGTCAAGGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MDQHHDSSGEAPMEIQSDLDFGPQAYTRDPDLDGDTLREECGVFGIYGHSDAAAITALGLHALQHRGQEAAGIVCYDGTR
FHSERRLGLVGDTFSRREVIERLPGTAAVGHVRYATTGATILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRAGA
IMQSTTDTEVILHLVAHSRRSNFIDRFIEALRALEGAYSLVCLTNKKLIGARDPLGIRPLVYGELDGCPILASETCALDM
IGARYIRDIEPGEVVIFDEAGMHSHKPFPPKPARPCVFEYIYFARPDSVVGGRSVYEVRKAFGAQLARESHPEVDVVVPV
PDSGVPAAIGYSQYSGVPFELGIIRNHYVGRTFIQPTQAIRESGVRMKHSANRAAIEGKRIILIDDSLVRGTTSRKIVRM
MRDAGATEVHFRLASPPILYPDYYGIDLPDRGGLLAATHSLEEMRELIGADSLAFLSIDGMYRAMGEPARDPAAPKYADH
CFTGSYPTSLTDQSLVEPTQQQLSLLAEAS

Sequences:

>Translated_510_residues
MDQHHDSSGEAPMEIQSDLDFGPQAYTRDPDLDGDTLREECGVFGIYGHSDAAAITALGLHALQHRGQEAAGIVCYDGTR
FHSERRLGLVGDTFSRREVIERLPGTAAVGHVRYATTGATILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRAGA
IMQSTTDTEVILHLVAHSRRSNFIDRFIEALRALEGAYSLVCLTNKKLIGARDPLGIRPLVYGELDGCPILASETCALDM
IGARYIRDIEPGEVVIFDEAGMHSHKPFPPKPARPCVFEYIYFARPDSVVGGRSVYEVRKAFGAQLARESHPEVDVVVPV
PDSGVPAAIGYSQYSGVPFELGIIRNHYVGRTFIQPTQAIRESGVRMKHSANRAAIEGKRIILIDDSLVRGTTSRKIVRM
MRDAGATEVHFRLASPPILYPDYYGIDLPDRGGLLAATHSLEEMRELIGADSLAFLSIDGMYRAMGEPARDPAAPKYADH
CFTGSYPTSLTDQSLVEPTQQQLSLLAEAS
>Mature_510_residues
MDQHHDSSGEAPMEIQSDLDFGPQAYTRDPDLDGDTLREECGVFGIYGHSDAAAITALGLHALQHRGQEAAGIVCYDGTR
FHSERRLGLVGDTFSRREVIERLPGTAAVGHVRYATTGATILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRAGA
IMQSTTDTEVILHLVAHSRRSNFIDRFIEALRALEGAYSLVCLTNKKLIGARDPLGIRPLVYGELDGCPILASETCALDM
IGARYIRDIEPGEVVIFDEAGMHSHKPFPPKPARPCVFEYIYFARPDSVVGGRSVYEVRKAFGAQLARESHPEVDVVVPV
PDSGVPAAIGYSQYSGVPFELGIIRNHYVGRTFIQPTQAIRESGVRMKHSANRAAIEGKRIILIDDSLVRGTTSRKIVRM
MRDAGATEVHFRLASPPILYPDYYGIDLPDRGGLLAATHSLEEMRELIGADSLAFLSIDGMYRAMGEPARDPAAPKYADH
CFTGSYPTSLTDQSLVEPTQQQLSLLAEAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=471, Percent_Identity=37.3673036093418, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1788651, Length=481, Percent_Identity=37.8378378378378, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI1790167, Length=238, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=38.045738045738, Blast_Score=311, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=35.9840954274354, Blast_Score=274, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=38.8329979879276, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=39.4456289978678, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=38.430583501006, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55653; Mature: 55653

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQHHDSSGEAPMEIQSDLDFGPQAYTRDPDLDGDTLREECGVFGIYGHSDAAAITALGL
CCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHH
HALQHRGQEAAGIVCYDGTRFHSERRLGLVGDTFSRREVIERLPGTAAVGHVRYATTGAT
HHHHHCCCCCCEEEEECCCEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHH
ILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRAGAIMQSTTDTEVILHLVAHSRR
HHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SNFIDRFIEALRALEGAYSLVCLTNKKLIGARDPLGIRPLVYGELDGCPILASETCALDM
HHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCEEECCCCHHHHH
IGARYIRDIEPGEVVIFDEAGMHSHKPFPPKPARPCVFEYIYFARPDSVVGGRSVYEVRK
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AFGAQLARESHPEVDVVVPVPDSGVPAAIGYSQYSGVPFELGIIRNHYVGRTFIQPTQAI
HHHHHHHHCCCCCEEEEEECCCCCCCCEECHHHCCCCCEEEEEECCCCCCCHHCCHHHHH
RESGVRMKHSANRAAIEGKRIILIDDSLVRGTTSRKIVRMMRDAGATEVHFRLASPPILY
HHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEEC
PDYYGIDLPDRGGLLAATHSLEEMRELIGADSLAFLSIDGMYRAMGEPARDPAAPKYADH
CCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHH
CFTGSYPTSLTDQSLVEPTQQQLSLLAEAS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQHHDSSGEAPMEIQSDLDFGPQAYTRDPDLDGDTLREECGVFGIYGHSDAAAITALGL
CCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHH
HALQHRGQEAAGIVCYDGTRFHSERRLGLVGDTFSRREVIERLPGTAAVGHVRYATTGAT
HHHHHCCCCCCEEEEECCCEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHH
ILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRAGAIMQSTTDTEVILHLVAHSRR
HHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SNFIDRFIEALRALEGAYSLVCLTNKKLIGARDPLGIRPLVYGELDGCPILASETCALDM
HHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCEEECCCCHHHHH
IGARYIRDIEPGEVVIFDEAGMHSHKPFPPKPARPCVFEYIYFARPDSVVGGRSVYEVRK
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AFGAQLARESHPEVDVVVPVPDSGVPAAIGYSQYSGVPFELGIIRNHYVGRTFIQPTQAI
HHHHHHHHCCCCCEEEEEECCCCCCCCEECHHHCCCCCEEEEEECCCCCCCHHCCHHHHH
RESGVRMKHSANRAAIEGKRIILIDDSLVRGTTSRKIVRMMRDAGATEVHFRLASPPILY
HHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEEC
PDYYGIDLPDRGGLLAATHSLEEMRELIGADSLAFLSIDGMYRAMGEPARDPAAPKYADH
CCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHH
CFTGSYPTSLTDQSLVEPTQQQLSLLAEAS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA