Click here to switch to the map view.

The map label for this gene is 146340117

Identifier: 146340117

GI number: 146340117

Start: 3328754

End: 3330313

Strand: Direct

Name: 146340117

Synonym: BRADO3132

Alternate gene names: NA

Gene position: 3328754-3330313 (Clockwise)

Preceding gene: 146340116

Following gene: 146340121

Centisome position: 44.64

GC content: 68.33

Gene sequence:

>1560_bases
ATGAGTTGGCAGCCGGAGCTCGAGGAATTGGCCCGGCGCGAGGCCTTCGCGCGGGAGATGGGCGGGCCTGACAAGGTCAA
GCGTCAGCACGACCAGGGCCGCCTCACCGTCCGCGAGCGGATCGACGGCGTCGTCGATCCCGGCAGCTTCCACGAGATCG
GCGCCGTCGCCGGTCTCGGTGAGTATGACGAGCAGGGCGAGCTCAAGCATGTCACGCCCGCGAACTGCGTGTTCGGCCGC
GCCCGCATCGACGGCCGCACCGTCGTCGTGGTCGGCGACGATTTCACCGTGCGCGGCGGCTCGGCCGATGCGTCGATCGC
GGCCAAGCCGCTGATGGCGGAGGAGATGGCGCACGACTTCCGTCTGCCCATCATCCGCATCATCGAGGGCTCCGGCGGCG
GCGGCTCGGTCAAGACCATCGAGACCAAGGGCGCGGCCAACCTGCCCGGCGGCATCGGCGGCAATCGCTGGTACCGCTAC
ACGACCGAGAACATGTCGCTGGTGCCGGTAATCGGCCTCGGTCTCGGCTCGGTGGCAGGCCTCGGCGCTGCGCGGCTCGC
GGCAAGCCACTACTCGGTCATGACCCGGCAGTCGGCGATGTTCGTCGCCGGTCCGCCGGTTGTGAAGCGGCTCGGCCAGG
ACCTCGGCAAGATGGAGCTCGGCGGCGCCGATATCCAGACCAAGGCGGGCGGCGTCGACCATGCCGTCGACACCGAAGCG
GAAGCCTTCGCCTGCGCGCGCCGCTTCCTGTCCTATCTGCCATCCTCGGTCTACGACCTACCGCCAACCTTGCCGTGCAC
CGATGATCCGCAGCGCAGTGACGAGTTGTTGATCAGCGCGGTGCCGCGCGATCGTCGCCGCGTCTACAAGATGCGGCCGA
TCATCGAATCCGTCGTCGACAGCGGCTCGTTCTTCGAGGTGGCGCAGAACTACGGCCGCCCGCTCATCGTCGGCCTCGCC
CGGCTCGAAGGCCGCGCGGTGATGGTGCTGGCGAGCGATCCCTATCACTTCGGTGGCTCGTGGACGGCAGATGCCTGCCA
GAAGGTGATCCGCTGGGTCGACTTCGCCGAGACCTTCCATCTGCCGATCGTCTATCTGATGGACTGTCCCGGCTTCATGG
TCGGCCTCGAGGCCGAGAAGGCGGCGACCATCCGCCATGGCGTGCGCGCGATGGCGGCGGTCAACCAGTCGACGGTGCCG
TGGTGCACGGTGATCGTGCGCAACTCGTTCGGTGTCGCCGGCGTCGTGCATCAGCCGGCGGACCGGTTCTCGATCCGCTA
CGCCTGGCCGTCGGCCTATTGGGGCTCGCTGCCGCTCGAAGGCGGCATCGAGGCGGCGTATCGTGCCGAGATCGATGCGG
CTCCGGATCCGAAGGCCAAGCTGAAGGAAATCGAGGAGCGGCTGAACAAGCTGCGCTCGCCGTTCCGCTCGGCCGAGAAG
TTCTGGGTCGAGGAGATCATCGACCCGCGCAAGACGCGCGCGCTGCTCTGCGAGTTCGCGCGTCTTGCCGAGCCGTTGCG
GACGCCGGGACCGCCGGCCGCGATGACAACGCGGCCGTAG

Upstream 100 bases:

>100_bases
GCGGAAGCCGACGTCTTCAACAGCCGGATGGAGAGAAAGCCGCTGCAGGTGACGACGAAGACCAATCATAAGAAGACCAT
CACCGGGGCAGGGGGAACGA

Downstream 100 bases:

>100_bases
TGAGTGTTACGCCGCGGCGGGCTTTGCCTGCCGCGCGATGGCGAGCGCGAGGAGCGCTGCGATGATGCAGAGCGCGCCGG
CGGTGAAGAACGCCGGCAGA

Product: putative methylmalonyl-CoA decarboxylase subunit alpha

Products: NA

Alternate protein names: Transcarboxylase 12S subunit [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSWQPELEELARREAFAREMGGPDKVKRQHDQGRLTVRERIDGVVDPGSFHEIGAVAGLGEYDEQGELKHVTPANCVFGR
ARIDGRTVVVVGDDFTVRGGSADASIAAKPLMAEEMAHDFRLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGNRWYRY
TTENMSLVPVIGLGLGSVAGLGAARLAASHYSVMTRQSAMFVAGPPVVKRLGQDLGKMELGGADIQTKAGGVDHAVDTEA
EAFACARRFLSYLPSSVYDLPPTLPCTDDPQRSDELLISAVPRDRRRVYKMRPIIESVVDSGSFFEVAQNYGRPLIVGLA
RLEGRAVMVLASDPYHFGGSWTADACQKVIRWVDFAETFHLPIVYLMDCPGFMVGLEAEKAATIRHGVRAMAAVNQSTVP
WCTVIVRNSFGVAGVVHQPADRFSIRYAWPSAYWGSLPLEGGIEAAYRAEIDAAPDPKAKLKEIEERLNKLRSPFRSAEK
FWVEEIIDPRKTRALLCEFARLAEPLRTPGPPAAMTTRP

Sequences:

>Translated_519_residues
MSWQPELEELARREAFAREMGGPDKVKRQHDQGRLTVRERIDGVVDPGSFHEIGAVAGLGEYDEQGELKHVTPANCVFGR
ARIDGRTVVVVGDDFTVRGGSADASIAAKPLMAEEMAHDFRLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGNRWYRY
TTENMSLVPVIGLGLGSVAGLGAARLAASHYSVMTRQSAMFVAGPPVVKRLGQDLGKMELGGADIQTKAGGVDHAVDTEA
EAFACARRFLSYLPSSVYDLPPTLPCTDDPQRSDELLISAVPRDRRRVYKMRPIIESVVDSGSFFEVAQNYGRPLIVGLA
RLEGRAVMVLASDPYHFGGSWTADACQKVIRWVDFAETFHLPIVYLMDCPGFMVGLEAEKAATIRHGVRAMAAVNQSTVP
WCTVIVRNSFGVAGVVHQPADRFSIRYAWPSAYWGSLPLEGGIEAAYRAEIDAAPDPKAKLKEIEERLNKLRSPFRSAEK
FWVEEIIDPRKTRALLCEFARLAEPLRTPGPPAAMTTRP
>Mature_518_residues
SWQPELEELARREAFAREMGGPDKVKRQHDQGRLTVRERIDGVVDPGSFHEIGAVAGLGEYDEQGELKHVTPANCVFGRA
RIDGRTVVVVGDDFTVRGGSADASIAAKPLMAEEMAHDFRLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGNRWYRYT
TENMSLVPVIGLGLGSVAGLGAARLAASHYSVMTRQSAMFVAGPPVVKRLGQDLGKMELGGADIQTKAGGVDHAVDTEAE
AFACARRFLSYLPSSVYDLPPTLPCTDDPQRSDELLISAVPRDRRRVYKMRPIIESVVDSGSFFEVAQNYGRPLIVGLAR
LEGRAVMVLASDPYHFGGSWTADACQKVIRWVDFAETFHLPIVYLMDCPGFMVGLEAEKAATIRHGVRAMAAVNQSTVPW
CTVIVRNSFGVAGVVHQPADRFSIRYAWPSAYWGSLPLEGGIEAAYRAEIDAAPDPKAKLKEIEERLNKLRSPFRSAEKF
WVEEIIDPRKTRALLCEFARLAEPLRTPGPPAAMTTRP

Specific function: The 12S subunit specifically catalyzes the transfer of the carboxyl group of methylmalonyl CoA to the biotin of the 1.3S subunit forming propanoyl-CoA and carboxylated 1.3S-biotin [H]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI119943100, Length=488, Percent_Identity=32.3770491803279, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI295821216, Length=508, Percent_Identity=31.1023622047244, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI11545863, Length=497, Percent_Identity=28.7726358148893, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25147359, Length=500, Percent_Identity=32, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI25147362, Length=500, Percent_Identity=32, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17552936, Length=508, Percent_Identity=28.740157480315, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24586065, Length=513, Percent_Identity=30.0194931773879, Blast_Score=185, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000438
- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: =2.1.3.1 [H]

Molecular weight: Translated: 56528; Mature: 56397

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQPELEELARREAFAREMGGPDKVKRQHDQGRLTVRERIDGVVDPGSFHEIGAVAGLG
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCCCCCCCCCHHHHHHHCCC
EYDEQGELKHVTPANCVFGRARIDGRTVVVVGDDFTVRGGSADASIAAKPLMAEEMAHDF
CCCCCCCCCCCCCCHHEEEEEEECCEEEEEECCCEEEECCCCCCHHHHCHHHHHHHHHHH
RLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGNRWYRYTTENMSLVPVIGLGLGSVAG
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHH
LGAARLAASHYSVMTRQSAMFVAGPPVVKRLGQDLGKMELGGADIQTKAGGVDHAVDTEA
CCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHH
EAFACARRFLSYLPSSVYDLPPTLPCTDDPQRSDELLISAVPRDRRRVYKMRPIIESVVD
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHC
SGSFFEVAQNYGRPLIVGLARLEGRAVMVLASDPYHFGGSWTADACQKVIRWVDFAETFH
CCCHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LPIVYLMDCPGFMVGLEAEKAATIRHGVRAMAAVNQSTVPWCTVIVRNSFGVAGVVHQPA
CCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCC
DRFSIRYAWPSAYWGSLPLEGGIEAAYRAEIDAAPDPKAKLKEIEERLNKLRSPFRSAEK
CCCEEEEECCCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FWVEEIIDPRKTRALLCEFARLAEPLRTPGPPAAMTTRP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SWQPELEELARREAFAREMGGPDKVKRQHDQGRLTVRERIDGVVDPGSFHEIGAVAGLG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCCCCCCCCCHHHHHHHCCC
EYDEQGELKHVTPANCVFGRARIDGRTVVVVGDDFTVRGGSADASIAAKPLMAEEMAHDF
CCCCCCCCCCCCCCHHEEEEEEECCEEEEEECCCEEEECCCCCCHHHHCHHHHHHHHHHH
RLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGNRWYRYTTENMSLVPVIGLGLGSVAG
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHH
LGAARLAASHYSVMTRQSAMFVAGPPVVKRLGQDLGKMELGGADIQTKAGGVDHAVDTEA
CCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHH
EAFACARRFLSYLPSSVYDLPPTLPCTDDPQRSDELLISAVPRDRRRVYKMRPIIESVVD
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHC
SGSFFEVAQNYGRPLIVGLARLEGRAVMVLASDPYHFGGSWTADACQKVIRWVDFAETFH
CCCHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LPIVYLMDCPGFMVGLEAEKAATIRHGVRAMAAVNQSTVPWCTVIVRNSFGVAGVVHQPA
CCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCC
DRFSIRYAWPSAYWGSLPLEGGIEAAYRAEIDAAPDPKAKLKEIEERLNKLRSPFRSAEK
CCCEEEEECCCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FWVEEIIDPRKTRALLCEFARLAEPLRTPGPPAAMTTRP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366018 [H]