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The map label for this gene is clcA [H]

Identifier: 146340086

GI number: 146340086

Start: 3289905

End: 3291215

Strand: Direct

Name: clcA [H]

Synonym: BRADO3095

Alternate gene names: 146340086

Gene position: 3289905-3291215 (Clockwise)

Preceding gene: 146340085

Following gene: 146340087

Centisome position: 44.12

GC content: 69.57

Gene sequence:

>1311_bases
ATGCTCAAGATCACCCCCCGCCGCAAGCGTTGGCTCAAGCTGACGTCGGCGCGCTGGCAGCGGCGGGTGCTGTTCGTGGC
GGGCGGTCTCGTGGTCGGCGCCGCCGCGGTCGCGCTTGCCAAGCTCGCCGATGAGGCCCAGCTCGCGTTCAACTGGCTGC
TGGCGCAATCCCGCTATGCGTCGTTGATCGTCACCCCCGCGGGCTTCGGCCTGTCGGTCTACCTGACGCAGCGCTTCTTC
CCGAACGCACAGGGCAGCGGCATTCCGCAGGCGATCGCCGCGCGTCATCTCACCGATACCAGTGCGCGCAGCCCGCTGGT
GTCGATCCGTATCGCCATCGGCAAGGTCCTGTTGACCCTGCTCGGGCTGCTCTGCGGCGGCTCGGTCGGGCGCGAGGGGC
CGACCGTCCAGGTCGGCGCCTCCATCATGTTCGCACTGGGCCGGCTGTCGCCGCGCCGCCAGCCCGGCCTGATCCTGGCC
GGCGCGGCCGCGGGCGTCGCCGCCGCCTTCAACACGCCGCTCGCCGGCATCGTGTTCGGCATTGAGGAGATGAGCCGCGC
CTTCGAGACGCGGACCTCCAGCCTCGTCATCGCGACCGTGATCGCGGCCGGCCTGACCTCGCTGGCGCTGATCGGCAACT
ACGCCTATTTCGGGACGACGCCGATTGCGCTCGGCAAAGGCATCGACTGGCTCGCCGTTCCGCTCTGCGGCGTGATCGGC
GGCGCCGCTGGCGGGCTGTTCAGCCGCATCCTGATCACCATGGCGCGGGGGCTGCCTGGAACCGCCGGCCTCTTCATCAA
GCGCCAGCCGATGCTCTTCGCGCTAGCCTGCGGCTTCGCCGTAGCGCTTTGCGGTCTCGCCTCGGGCGGCACGGTCTACG
GCACCGGCTACGCCCAGGTGAAGACGGCGCTCGAGAGCGGCACGCCGCTGCCCGCCGATTTCGGCGCGCTGAAGTTCTTG
GCCACGACCTTCGCTGCGATCAGCGGCATTCCCGGCGGCATCTTCGCGCCGTCGCTCGCCGTTGGCGCCGGCATCGGCGC
CAATGTCGCCAGCCTGTTCCAGCACACGCCGCTGGCGCCGATCATGCTGCTCGGCATGGTCGCCTATTTCGCCGGCGTGG
TGCAGGCGCCGATCACCGCCTTCGTCATCGTCACCGAGATGACCGACAACCACGCCATGGTGGTGCCGCTGATGATGGCC
TCGCTGATCGCCTACGGCACCTCGCGACTGATCTGCGAGGAGGGGCTCTATCACGCGCTCGCCAAAGGTTTTGTCGCGAA
GGCGGAGGCGGGAGCGGCGGGCCATCTGTAG

Upstream 100 bases:

>100_bases
GTATGCCGAAGCGCTGCGCGACCGCTGGAACGCACTGGACCGTCGGCTGATTTTCGTCGAAGGTGTTGCACCTGCGTAAA
ATCCGGACCGTAGCGCGCTC

Downstream 100 bases:

>100_bases
CCCGGATGAGCGACAGCGACATCCGGGTTCATCGCCGCAGCTCGTGTGGCCCCGGATGTGGCTGCGCTCATCCGGGCTCC
GCACTCCTCGTCACCTTGCA

Product: putative H(+)/Cl(-) exchange transporter

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLKITPRRKRWLKLTSARWQRRVLFVAGGLVVGAAAVALAKLADEAQLAFNWLLAQSRYASLIVTPAGFGLSVYLTQRFF
PNAQGSGIPQAIAARHLTDTSARSPLVSIRIAIGKVLLTLLGLLCGGSVGREGPTVQVGASIMFALGRLSPRRQPGLILA
GAAAGVAAAFNTPLAGIVFGIEEMSRAFETRTSSLVIATVIAAGLTSLALIGNYAYFGTTPIALGKGIDWLAVPLCGVIG
GAAGGLFSRILITMARGLPGTAGLFIKRQPMLFALACGFAVALCGLASGGTVYGTGYAQVKTALESGTPLPADFGALKFL
ATTFAAISGIPGGIFAPSLAVGAGIGANVASLFQHTPLAPIMLLGMVAYFAGVVQAPITAFVIVTEMTDNHAMVVPLMMA
SLIAYGTSRLICEEGLYHALAKGFVAKAEAGAAGHL

Sequences:

>Translated_436_residues
MLKITPRRKRWLKLTSARWQRRVLFVAGGLVVGAAAVALAKLADEAQLAFNWLLAQSRYASLIVTPAGFGLSVYLTQRFF
PNAQGSGIPQAIAARHLTDTSARSPLVSIRIAIGKVLLTLLGLLCGGSVGREGPTVQVGASIMFALGRLSPRRQPGLILA
GAAAGVAAAFNTPLAGIVFGIEEMSRAFETRTSSLVIATVIAAGLTSLALIGNYAYFGTTPIALGKGIDWLAVPLCGVIG
GAAGGLFSRILITMARGLPGTAGLFIKRQPMLFALACGFAVALCGLASGGTVYGTGYAQVKTALESGTPLPADFGALKFL
ATTFAAISGIPGGIFAPSLAVGAGIGANVASLFQHTPLAPIMLLGMVAYFAGVVQAPITAFVIVTEMTDNHAMVVPLMMA
SLIAYGTSRLICEEGLYHALAKGFVAKAEAGAAGHL
>Mature_436_residues
MLKITPRRKRWLKLTSARWQRRVLFVAGGLVVGAAAVALAKLADEAQLAFNWLLAQSRYASLIVTPAGFGLSVYLTQRFF
PNAQGSGIPQAIAARHLTDTSARSPLVSIRIAIGKVLLTLLGLLCGGSVGREGPTVQVGASIMFALGRLSPRRQPGLILA
GAAAGVAAAFNTPLAGIVFGIEEMSRAFETRTSSLVIATVIAAGLTSLALIGNYAYFGTTPIALGKGIDWLAVPLCGVIG
GAAGGLFSRILITMARGLPGTAGLFIKRQPMLFALACGFAVALCGLASGGTVYGTGYAQVKTALESGTPLPADFGALKFL
ATTFAAISGIPGGIFAPSLAVGAGIGANVASLFQHTPLAPIMLLGMVAYFAGVVQAPITAFVIVTEMTDNHAMVVPLMMA
SLIAYGTSRLICEEGLYHALAKGFVAKAEAGAAGHL

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=448, Percent_Identity=31.25, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 44727; Mature: 44727

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKITPRRKRWLKLTSARWQRRVLFVAGGLVVGAAAVALAKLADEAQLAFNWLLAQSRYA
CCCCCCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLIVTPAGFGLSVYLTQRFFPNAQGSGIPQAIAARHLTDTSARSPLVSIRIAIGKVLLTL
EEEEECCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGLLCGGSVGREGPTVQVGASIMFALGRLSPRRQPGLILAGAAAGVAAAFNTPLAGIVFG
HHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHCCCHHHHHHH
IEEMSRAFETRTSSLVIATVIAAGLTSLALIGNYAYFGTTPIALGKGIDWLAVPLCGVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCHHHHHHHHHHHH
GAAGGLFSRILITMARGLPGTAGLFIKRQPMLFALACGFAVALCGLASGGTVYGTGYAQV
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
KTALESGTPLPADFGALKFLATTFAAISGIPGGIFAPSLAVGAGIGANVASLFQHTPLAP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHCCCCHHHHHHHHCCCCHH
IMLLGMVAYFAGVVQAPITAFVIVTEMTDNHAMVVPLMMASLIAYGTSRLICEEGLYHAL
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKGFVAKAEAGAAGHL
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKITPRRKRWLKLTSARWQRRVLFVAGGLVVGAAAVALAKLADEAQLAFNWLLAQSRYA
CCCCCCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLIVTPAGFGLSVYLTQRFFPNAQGSGIPQAIAARHLTDTSARSPLVSIRIAIGKVLLTL
EEEEECCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGLLCGGSVGREGPTVQVGASIMFALGRLSPRRQPGLILAGAAAGVAAAFNTPLAGIVFG
HHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHCCCHHHHHHH
IEEMSRAFETRTSSLVIATVIAAGLTSLALIGNYAYFGTTPIALGKGIDWLAVPLCGVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCHHHHHHHHHHHH
GAAGGLFSRILITMARGLPGTAGLFIKRQPMLFALACGFAVALCGLASGGTVYGTGYAQV
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
KTALESGTPLPADFGALKFLATTFAAISGIPGGIFAPSLAVGAGIGANVASLFQHTPLAP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHCCCCHHHHHHHHCCCCHH
IMLLGMVAYFAGVVQAPITAFVIVTEMTDNHAMVVPLMMASLIAYGTSRLICEEGLYHAL
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKGFVAKAEAGAAGHL
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA