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The map label for this gene is 146340012

Identifier: 146340012

GI number: 146340012

Start: 3215753

End: 3216709

Strand: Direct

Name: 146340012

Synonym: BRADO3018

Alternate gene names: NA

Gene position: 3215753-3216709 (Clockwise)

Preceding gene: 146340011

Following gene: 146340013

Centisome position: 43.13

GC content: 65.1

Gene sequence:

>957_bases
ATGCCGCGCTTCGTCAACGTCGCCATCGGCCAGCTCGGCCCCATCGCCAGGAGCGAGCCGCGCAGCGTGGTCGTCGCGCG
GCTGATCGCCCTGATGCGTCAGGCGCACGCCAACGGCTGCGACCTCATCGTCTATCCCGAGCTCGCGCTGACGACCTTCT
TCCCGCGCTGGTACATGGCCGATCAGGCCGAGATCGATACGTACTTCGAGCGCGAGATGCCCGGGCCGGAGACGCAGGCG
CTCTTCGCGCTCACGAAGGAGCTGCGGATCGGCTTCTGCCTGGGCTACGCCGAGCTGACGGTCGAAGATGGCGTCGTGCA
CCGCTACAACACCGCCATCCTCGTCGACAAGGACGCGCGCATCGTCTCGAAATACCGCAAGGTGCATCTGCCGGGACATG
CCGAGCACGAACCGTGGCGCAAGTTCCAGCATCTGGAAAAGCGTTACTTCGAGCCGGGCAGGGGTTTCGGCGTGGCCGAT
GCCTTCGGCGGCGTCATCGGCATGGCGATCTGCAACGACCGGCGCTGGCCCGAGACCTATCGCGTGATGGGCCTGCAAGG
TGTGGAGATGGTGCTGATCGGCTACAACACGCCGGTGCACAATCCGCCTGCGCCGGAGCACGATGATCTCTCGCTGTTCC
ACAACCGCCTCGTGATGCAGTCCGGTGCCTATCAGAACGGCACCTGGGTGATCGGCGTGGCGAAGGGCGGCATCGAGGAG
GGCGTCGATCATATCGCCGGCAGCTGCATCATCGCGCCCTCAGGCGAAATCGTGGCGGCCTGCGCTACCAAGGGCGACGA
GATCGCGCTGGCCCGCTGCGATCTCGACCTGTGCAAGTCGTACAAGCAGACGATCTTCAATTTCGACATCCATCGCCAGC
CCCGGGCTTACGGCATGATCGTCGAGCGCAAGGGCGAGCGGCTGATGGCGGACGGTTCGCCGGTGCGGAAATCGTGA

Upstream 100 bases:

>100_bases
TATGCGCCGGGCTTGCGCGAGTAGAGCCGCGTGGCATGATGACTGCAGGAGCAGCGCACCCCGCGAACCACAAAACAGTT
CCGGAAAAAGGAACAGAATC

Downstream 100 bases:

>100_bases
CGCGTAGGGTGGGCAAAGGCGCTGCCGTGCTGTTTCCTGATCCAAGATCGCAATGGCGCCGTGCCCATCGTCTCGCTGCG
GACTGCGCGGCGCGATGGTG

Product: N-carbamoyl-D-amino acid hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPRFVNVAIGQLGPIARSEPRSVVVARLIALMRQAHANGCDLIVYPELALTTFFPRWYMADQAEIDTYFEREMPGPETQA
LFALTKELRIGFCLGYAELTVEDGVVHRYNTAILVDKDARIVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVAD
AFGGVIGMAICNDRRWPETYRVMGLQGVEMVLIGYNTPVHNPPAPEHDDLSLFHNRLVMQSGAYQNGTWVIGVAKGGIEE
GVDHIAGSCIIAPSGEIVAACATKGDEIALARCDLDLCKSYKQTIFNFDIHRQPRAYGMIVERKGERLMADGSPVRKS

Sequences:

>Translated_318_residues
MPRFVNVAIGQLGPIARSEPRSVVVARLIALMRQAHANGCDLIVYPELALTTFFPRWYMADQAEIDTYFEREMPGPETQA
LFALTKELRIGFCLGYAELTVEDGVVHRYNTAILVDKDARIVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVAD
AFGGVIGMAICNDRRWPETYRVMGLQGVEMVLIGYNTPVHNPPAPEHDDLSLFHNRLVMQSGAYQNGTWVIGVAKGGIEE
GVDHIAGSCIIAPSGEIVAACATKGDEIALARCDLDLCKSYKQTIFNFDIHRQPRAYGMIVERKGERLMADGSPVRKS
>Mature_317_residues
PRFVNVAIGQLGPIARSEPRSVVVARLIALMRQAHANGCDLIVYPELALTTFFPRWYMADQAEIDTYFEREMPGPETQAL
FALTKELRIGFCLGYAELTVEDGVVHRYNTAILVDKDARIVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVADA
FGGVIGMAICNDRRWPETYRVMGLQGVEMVLIGYNTPVHNPPAPEHDDLSLFHNRLVMQSGAYQNGTWVIGVAKGGIEEG
VDHIAGSCIIAPSGEIVAACATKGDEIALARCDLDLCKSYKQTIFNFDIHRQPRAYGMIVERKGERLMADGSPVRKS

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=294, Percent_Identity=24.4897959183673, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 35530; Mature: 35399

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFVNVAIGQLGPIARSEPRSVVVARLIALMRQAHANGCDLIVYPELALTTFFPRWYMA
CCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHHCC
DQAEIDTYFEREMPGPETQALFALTKELRIGFCLGYAELTVEDGVVHRYNTAILVDKDAR
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEEEEEECCEEEEECEEEEEECCHH
IVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVADAFGGVIGMAICNDRRWPETY
HHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
RVMGLQGVEMVLIGYNTPVHNPPAPEHDDLSLFHNRLVMQSGAYQNGTWVIGVAKGGIEE
HEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHH
GVDHIAGSCIIAPSGEIVAACATKGDEIALARCDLDLCKSYKQTIFNFDIHRQPRAYGMI
HHHHHCCCEEECCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHEECCCCCCCCCEEEE
VERKGERLMADGSPVRKS
EECCCCEEECCCCCCCCC
>Mature Secondary Structure 
PRFVNVAIGQLGPIARSEPRSVVVARLIALMRQAHANGCDLIVYPELALTTFFPRWYMA
CCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHHCC
DQAEIDTYFEREMPGPETQALFALTKELRIGFCLGYAELTVEDGVVHRYNTAILVDKDAR
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEEEEEECCEEEEECEEEEEECCHH
IVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVADAFGGVIGMAICNDRRWPETY
HHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
RVMGLQGVEMVLIGYNTPVHNPPAPEHDDLSLFHNRLVMQSGAYQNGTWVIGVAKGGIEE
HEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHH
GVDHIAGSCIIAPSGEIVAACATKGDEIALARCDLDLCKSYKQTIFNFDIHRQPRAYGMI
HHHHHCCCEEECCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHEECCCCCCCCCEEEE
VERKGERLMADGSPVRKS
EECCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]