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The map label for this gene is groEL [H]

Identifier: 146339700

GI number: 146339700

Start: 2896486

End: 2898105

Strand: Direct

Name: groEL [H]

Synonym: BRADO2697

Alternate gene names: 146339700

Gene position: 2896486-2898105 (Clockwise)

Preceding gene: 146339699

Following gene: 146339701

Centisome position: 38.84

GC content: 66.6

Gene sequence:

>1620_bases
ATGGCTGCCAAGGACGTGAAGTTTTCCACCGACGCGCGCGACCGTATGCTGCGCGGCGTCGACATCCTCGCCAATGCCGT
CAAGGTCACGCTCGGTCCCAAGGGCCGCAACGTCGTGATCGAGAAATCGTTCGGTGCGCCGCGCATCACCAAGGATGGGG
TGACGGTCGCCAAGGAGATCGAGCTGGAGGACAAGTTCGAGAACATGGGCGCGCAGATGGTGCGCGAAGTGGCCTCGAAG
ACCGCCGATCTCGCCGGCGACGGCACCACCACCGCCACCGTGCTCGCCCAGGCGATCGTGAAGGAAGGCGCGAAGTCTGT
CGCCGCCGGCATGAACCCGATGGACCTCAAGCGCGGCATCGATCTCGCGGTCGAGGCGATTGTGAAAGACCTCAAGGCGC
ACGCCAAGAAGATCACCAGCAACGACGAGATCGCCCAGGTCGGCACCATCTCGGCCAATGGCGACAGCGAGATCGGCCGT
TTCCTTGCGGAAGCCATGCAGAAGGTCGGCAATGAGGGCGTGATCACGGTGGAGGAGGCGAAGAGCCTCGACACCGAGCT
CGAGGTGGTCGAGGGCATGCAGTTCGACCGCGGCTATGTCTCGCCATACTTCGTCACCAACTCCGAGAAGATGCGGGTCG
AGCTCGAGGATCCCTACATCCTGATCCACGAGAAGAAGCTGTCGGGCCTGCAGACGATGCTGCCGCTGCTCGAAGCCGTC
GTGCAGTCCGGCAAGCCGCTGCTGATCGTCGCCGAGGATGTCGAGGGCGAGGCGCTGGCGACCCTGGTGGTCAACAAGCT
GCGCGGCGGACTGAAGATCGCGGCCGTCAAGGCGCCGGGCTTCGGCGACCGCCGCAAGGCCATGCTGGAGGACATCGCGA
TCCTCACCGGCGGCACCACGATCTCCGAGGATCTCGGCATCAAGCTGGAGAACGTCACCCTGTCGATGCTCGGCCGCGCC
AAGAAGGTCGTGATCGACAAGGAGAACACGACGATCGTCGACGGTGCCGGCGCGAAGAAGGACATCGAGGCGCGCACGCA
GCAGATCCGGCTGCAGATCGAGGAGACCACCTCCGACTACGACCGCGAGAAGCTGCAGGAGCGGCTGGCCAAGCTCGCCG
GCGGCGTCGCGGTGATCCGGGTCGGCGGCGCCACCGAGGTCGAGGTCAAGGAGCGCAAGGACCGCGTCGACGATGCGCTG
CACGCCACGCGCGCGGCGGTGGAGGAGGGTATCCTGCCCGGTGGTGGCGTCGCGCTGCTGCGCGCCACCAAGGTGCTCGA
CGGCGTCAAGACCGCCAACGCCGACCAGAAAGCCGGCGTCGACATCATCCGGCGCGCGATCCAGGTGCCGGTGCGCCAGA
TCGTGCAGAATGCCGGCGACGACGGCTCGCTGGTGGTCGGCAAGCTCCTGGAGAAGGACAGCTACAGCTGGGGCTTCAAC
GCCGCGACCGGCGAGTACCAGGACCTGGTGCAGGCCGGCGTGATCGATCCGGCCAAGGTGGTCCGCACCGCGCTGCAGGA
CGCGGCCTCGGTCGCCTCGCTGCTGATCACCACCGAGGCGCTGGTGGCCGATAAGCCGAAGAAGGCGGAGACCGCCGCCG
CGCCGGCGATGGACTACTGA

Upstream 100 bases:

>100_bases
GAGAGCGACCTGCTCGGCATCGTCGACGCCCCCGTCGCGGCGAAGAAAGCCGCGTGATCCCTCAAACTGACAGTCATCGG
TAGACAAGGAGACGATGAAC

Downstream 100 bases:

>100_bases
TCGCGATCAGCCGTGACGGACTGCGCCAGCGCAGCCGTCACGGCTTCAAAAGAGCGGTTTCAAAAGAAAGTCTGTTGTTC
GCATCGCCGGCCGCGGATCG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60 1 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAAKDVKFSTDARDRMLRGVDILANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASK
TADLAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAIVKDLKAHAKKITSNDEIAQVGTISANGDSEIGR
FLAEAMQKVGNEGVITVEEAKSLDTELEVVEGMQFDRGYVSPYFVTNSEKMRVELEDPYILIHEKKLSGLQTMLPLLEAV
VQSGKPLLIVAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGTTISEDLGIKLENVTLSMLGRA
KKVVIDKENTTIVDGAGAKKDIEARTQQIRLQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAL
HATRAAVEEGILPGGGVALLRATKVLDGVKTANADQKAGVDIIRRAIQVPVRQIVQNAGDDGSLVVGKLLEKDSYSWGFN
AATGEYQDLVQAGVIDPAKVVRTALQDAASVASLLITTEALVADKPKKAETAAAPAMDY

Sequences:

>Translated_539_residues
MAAKDVKFSTDARDRMLRGVDILANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASK
TADLAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAIVKDLKAHAKKITSNDEIAQVGTISANGDSEIGR
FLAEAMQKVGNEGVITVEEAKSLDTELEVVEGMQFDRGYVSPYFVTNSEKMRVELEDPYILIHEKKLSGLQTMLPLLEAV
VQSGKPLLIVAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGTTISEDLGIKLENVTLSMLGRA
KKVVIDKENTTIVDGAGAKKDIEARTQQIRLQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDAL
HATRAAVEEGILPGGGVALLRATKVLDGVKTANADQKAGVDIIRRAIQVPVRQIVQNAGDDGSLVVGKLLEKDSYSWGFN
AATGEYQDLVQAGVIDPAKVVRTALQDAASVASLLITTEALVADKPKKAETAAAPAMDY
>Mature_538_residues
AAKDVKFSTDARDRMLRGVDILANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKT
ADLAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGIDLAVEAIVKDLKAHAKKITSNDEIAQVGTISANGDSEIGRF
LAEAMQKVGNEGVITVEEAKSLDTELEVVEGMQFDRGYVSPYFVTNSEKMRVELEDPYILIHEKKLSGLQTMLPLLEAVV
QSGKPLLIVAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGTTISEDLGIKLENVTLSMLGRAK
KVVIDKENTTIVDGAGAKKDIEARTQQIRLQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDALH
ATRAAVEEGILPGGGVALLRATKVLDGVKTANADQKAGVDIIRRAIQVPVRQIVQNAGDDGSLVVGKLLEKDSYSWGFNA
ATGEYQDLVQAGVIDPAKVVRTALQDAASVASLLITTEALVADKPKKAETAAAPAMDY

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=54.6489563567362, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=54.6489563567362, Blast_Score=583, Evalue=1e-166,
Organism=Escherichia coli, GI1790586, Length=527, Percent_Identity=66.4136622390892, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=53.7001897533207, Blast_Score=570, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI193210679, Length=209, Percent_Identity=53.5885167464115, Blast_Score=224, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=58.5551330798479, Blast_Score=626, Evalue=1e-180,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=57.1157495256167, Blast_Score=608, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=57.1157495256167, Blast_Score=608, Evalue=1e-174,
Organism=Drosophila melanogaster, GI45550936, Length=527, Percent_Identity=52.9411764705882, Blast_Score=567, Evalue=1e-162,
Organism=Drosophila melanogaster, GI45550132, Length=527, Percent_Identity=52.9411764705882, Blast_Score=567, Evalue=1e-162,
Organism=Drosophila melanogaster, GI45550935, Length=527, Percent_Identity=52.9411764705882, Blast_Score=567, Evalue=1e-162,
Organism=Drosophila melanogaster, GI17864606, Length=526, Percent_Identity=48.4790874524715, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=37.2400756143667, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=37.2400756143667, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57415; Mature: 57283

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVKFSTDARDRMLRGVDILANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCCEEHHC
ELEDKFENMGAQMVREVASKTADLAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DLAVEAIVKDLKAHAKKITSNDEIAQVGTISANGDSEIGRFLAEAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLDTELEVVEGMQFDRGYVSPYFVTNSEKMRVELEDPYILIHEKKLSGLQTMLPLLEAV
HCCCHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHH
VQSGKPLLIVAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGTT
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHEECCCE
ISEDLGIKLENVTLSMLGRAKKVVIDKENTTIVDGAGAKKDIEARTQQIRLQIEETTSDY
ECCCCCCEEEEEHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHEEEEECCCCCC
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RATKVLDGVKTANADQKAGVDIIRRAIQVPVRQIVQNAGDDGSLVVGKLLEKDSYSWGFN
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
AATGEYQDLVQAGVIDPAKVVRTALQDAASVASLLITTEALVADKPKKAETAAAPAMDY
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
AAKDVKFSTDARDRMLRGVDILANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCCEEHHC
ELEDKFENMGAQMVREVASKTADLAGDGTTTATVLAQAIVKEGAKSVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DLAVEAIVKDLKAHAKKITSNDEIAQVGTISANGDSEIGRFLAEAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLDTELEVVEGMQFDRGYVSPYFVTNSEKMRVELEDPYILIHEKKLSGLQTMLPLLEAV
HCCCHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHH
VQSGKPLLIVAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGTT
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHEECCCE
ISEDLGIKLENVTLSMLGRAKKVVIDKENTTIVDGAGAKKDIEARTQQIRLQIEETTSDY
ECCCCCCEEEEEHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHEEEEECCCCCC
DREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RATKVLDGVKTANADQKAGVDIIRRAIQVPVRQIVQNAGDDGSLVVGKLLEKDSYSWGFN
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
AATGEYQDLVQAGVIDPAKVVRTALQDAASVASLLITTEALVADKPKKAETAAAPAMDY
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8808920; 12597275 [H]