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The map label for this gene is paaE [H]

Identifier: 146339533

GI number: 146339533

Start: 2695905

End: 2696918

Strand: Direct

Name: paaE [H]

Synonym: BRADO2523

Alternate gene names: 146339533

Gene position: 2695905-2696918 (Clockwise)

Preceding gene: 146339532

Following gene: 146339536

Centisome position: 36.15

GC content: 66.96

Gene sequence:

>1014_bases
GTGTCGCTGACGTTTGCCATTCCAGGCGAGCTGGTCGACGATTATCGCTTCGCGCCCGGGCAATATCTCACGTTGAAGAC
CACGCTCGGCGGCGAAGAGGTCCGGCGGTCCTATTCGATCTGCTCCGGTCCCGATGACAACGAGATCCGCATCGCGGTGA
AGCGCGTCGATGGCGGCGCGTTCTCCAGCTTCGTCACCGACGAATTGAAGCGCGGCGACGAGATCGACGTGATGACGCCG
ACCGGCCGCTTCGGCGTGGCGCTGCAGCCGGAGGCCGCGCGCACCCATGTCGGCTTTGCGGCGGGATCGGGCATCACGCC
GATCCTGTCGATCGTCAAAGCCGTGCTGGCGCGCGAGCCGGACAGCCGCTTCTTCCTGTTCTATGGCAACCGCACCACCG
CGGGCATGCTGTTCCGCGAGCAGCTCGAGGAGCTGAAGGACCGCTTCCTCGATCGCCTCTCGGTGTTCCACGTCATCTCC
GGCGAGGAGCAGGATCTGCCGATTCTGCACGGCCGTCTCGACGGCGAGAAGGTGCGCCTGCTGTTGCGCTCGCTGGTGCC
GGCCGAGGCTGTTGATCACGTCTTCATCTGCGGCCCCACCGGGATGAGCGAGGAGATCGAGGCCGCCTGCCGTGCGCTCG
GCATCGCCGACGACCGCGTCCATGTCGAGCGCTTCGTCTCCGGCGCTGGCGGCAAGCCGCGGCCGAAGATCGTGGTGCCC
GACTCCGCACCGCCGAAAGCGATCGCCGCCCTGATCATCGACGGCAAACGCCGCGAGGTGCCGGTCGCCGAGGGCGAGGC
CATTCTCGACGCCGCGCTGCGCGCCGGTGTCGATTTGCCGTTCGCCTGCAAGGGCGGCATGTGCTCCACCTGCCGCGCCA
AGCTCTCCGAGGGCAAGGCCGAGATGGAGCTGAACTACTCGCTCGAGCCGTGGGAATTGCAGGCCGGCTTCGTGCTGACC
TGCCAGGCGAAGCCGGTGACGGAGCGGGTGGTGGTGGACTACGATCATATGTGA

Upstream 100 bases:

>100_bases
TCGCTTCGCTCGCAATGACGCGGGGATAGAATCGATGTCGACAACGCCACGCTTCCATCGTCTTGCCGTCCGTGACTTGC
GCCGCGAGGCGCCGGATGCG

Downstream 100 bases:

>100_bases
GAGGGCGTGTCCGCAATCTCGCTGCTGATAAAATATCGATGTGAGCACGCTCTCACGGCGGGCTCGGTGCAACCTCGCCC
CGCTTGCGGAGAGAGGTCGG

Product: putative phenylacetic acid degradation NADH oxidoreductase PaaE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSLTFAIPGELVDDYRFAPGQYLTLKTTLGGEEVRRSYSICSGPDDNEIRIAVKRVDGGAFSSFVTDELKRGDEIDVMTP
TGRFGVALQPEAARTHVGFAAGSGITPILSIVKAVLAREPDSRFFLFYGNRTTAGMLFREQLEELKDRFLDRLSVFHVIS
GEEQDLPILHGRLDGEKVRLLLRSLVPAEAVDHVFICGPTGMSEEIEAACRALGIADDRVHVERFVSGAGGKPRPKIVVP
DSAPPKAIAALIIDGKRREVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLSEGKAEMELNYSLEPWELQAGFVLT
CQAKPVTERVVVDYDHM

Sequences:

>Translated_337_residues
MSLTFAIPGELVDDYRFAPGQYLTLKTTLGGEEVRRSYSICSGPDDNEIRIAVKRVDGGAFSSFVTDELKRGDEIDVMTP
TGRFGVALQPEAARTHVGFAAGSGITPILSIVKAVLAREPDSRFFLFYGNRTTAGMLFREQLEELKDRFLDRLSVFHVIS
GEEQDLPILHGRLDGEKVRLLLRSLVPAEAVDHVFICGPTGMSEEIEAACRALGIADDRVHVERFVSGAGGKPRPKIVVP
DSAPPKAIAALIIDGKRREVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLSEGKAEMELNYSLEPWELQAGFVLT
CQAKPVTERVVVDYDHM
>Mature_336_residues
SLTFAIPGELVDDYRFAPGQYLTLKTTLGGEEVRRSYSICSGPDDNEIRIAVKRVDGGAFSSFVTDELKRGDEIDVMTPT
GRFGVALQPEAARTHVGFAAGSGITPILSIVKAVLAREPDSRFFLFYGNRTTAGMLFREQLEELKDRFLDRLSVFHVISG
EEQDLPILHGRLDGEKVRLLLRSLVPAEAVDHVFICGPTGMSEEIEAACRALGIADDRVHVERFVSGAGGKPRPKIVVPD
SAPPKAIAALIIDGKRREVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLSEGKAEMELNYSLEPWELQAGFVLTC
QAKPVTERVVVDYDHM

Specific function: May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI49574502, Length=218, Percent_Identity=27.5229357798165, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1787658, Length=336, Percent_Identity=46.7261904761905, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1787098, Length=314, Percent_Identity=26.7515923566879, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788104, Length=322, Percent_Identity=26.7080745341615, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788903, Length=223, Percent_Identity=26.0089686098655, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563926, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323552, Length=205, Percent_Identity=25.8536585365854, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=202, Percent_Identity=27.2277227722772, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR011884
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36692; Mature: 36560

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTFAIPGELVDDYRFAPGQYLTLKTTLGGEEVRRSYSICSGPDDNEIRIAVKRVDGGA
CEEEEECCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCH
FSSFVTDELKRGDEIDVMTPTGRFGVALQPEAARTHVGFAAGSGITPILSIVKAVLAREP
HHHHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHEECEECCCCHHHHHHHHHHHHHCCC
DSRFFLFYGNRTTAGMLFREQLEELKDRFLDRLSVFHVISGEEQDLPILHGRLDGEKVRL
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCHHHHHH
LLRSLVPAEAVDHVFICGPTGMSEEIEAACRALGIADDRVHVERFVSGAGGKPRPKIVVP
HHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEC
DSAPPKAIAALIIDGKRREVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLSEGKA
CCCCHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
EMELNYSLEPWELQAGFVLTCQAKPVTERVVVDYDHM
EEEEECCCCCEEECCCEEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure 
SLTFAIPGELVDDYRFAPGQYLTLKTTLGGEEVRRSYSICSGPDDNEIRIAVKRVDGGA
EEEEECCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCH
FSSFVTDELKRGDEIDVMTPTGRFGVALQPEAARTHVGFAAGSGITPILSIVKAVLAREP
HHHHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHEECEECCCCHHHHHHHHHHHHHCCC
DSRFFLFYGNRTTAGMLFREQLEELKDRFLDRLSVFHVISGEEQDLPILHGRLDGEKVRL
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCHHHHHH
LLRSLVPAEAVDHVFICGPTGMSEEIEAACRALGIADDRVHVERFVSGAGGKPRPKIVVP
HHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEC
DSAPPKAIAALIIDGKRREVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLSEGKA
CCCCHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
EMELNYSLEPWELQAGFVLTCQAKPVTERVVVDYDHM
EEEEECCCCCEEECCCEEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]