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The map label for this gene is hipO [H]

Identifier: 146339008

GI number: 146339008

Start: 2080154

End: 2081326

Strand: Direct

Name: hipO [H]

Synonym: BRADO1957

Alternate gene names: 146339008

Gene position: 2080154-2081326 (Clockwise)

Preceding gene: 146339007

Following gene: 146339009

Centisome position: 27.9

GC content: 67.52

Gene sequence:

>1173_bases
ATGCCCATCCTGAACAGCATCGCCGCGCTCACCGACGACATGGCGGCCTGGCGCCACGATTTTCATGAACATCCGGAGCT
GATGTACGAGGTGCACCGCACCGCCGGCATCGTCGCCGAGCGCCTGCGCGAATTTGGCTGCGACGAGGTCGTGACGGGGA
TCGGACGCACCGGCGTGGTCGGGGTCATCCGCGGCCGCAAAACAGATTCGGGCCGCACCATCGGCCTGCGCGCCGACATG
GACGCGCTACCGATCGAGGAAGCCTCGGGCGTGCCCTATGCCTCGAAGACGCCCGGCCTGATGCATGCCTGCGGCCATGA
CGGCCACACCGCGATGCTGCTCGGTGCCGCCAAACATCTCGCCGACACCCGCAATTTCGACGGCACCGCGATCGTGATCT
TCCAGCCGGCCGAGGAGGGCGGTGCGGGCGGCAAGGCGATGGTCGACGACGGCCTGATGACGCGCTGGGGCATCCAGGAG
GTCTACGGCCTGCACAACGCGCCCGGCGTGCCCGAGGGCGTGTTCGCGCTGCGCCCGGGCCCGATGCTGGCCTCGTCGGA
TCAACTCGAGATCACCGTGCATGGCAAGGGCGGCCATGCCGGTGCCGGCGTGCACAAGGCGGTCGACAGCGTGCTGATCG
CCGCGCACATCATCACTGCGCTGCAGTCGATCGTGTCGCGCAATGTCGATCCGATCAAATCCGCGGTCATTTCGATCTGC
ATGGTCGAGGCCGGCAAGGCAATGAACGTGATCCCCGAGACGGTCGAGCTGAAGGGCACCGTGCGCACGCTCGATCCCGA
GGTGCAGGACCTGGTCGAGCGTCGCATCGCCGAGGTCGCCGACGGTATTGCGCGCACCTATGGCGGCACGGCGACGACGA
AATACACGCGGCTCTATCCGATCACCATGAACCATGCGCGCGAAACCGGCGTGGCCGCCGATGTCGCCCGCGACGTGGTC
GGCGCCGAGCGCGTCGTCGACAACGCCCCGCCGCTGATGGGCGCCGAGGATTTCGCCTTCATGCTGCAGGCCCGCCCGGG
CGCCTTCGTCTTCCTCGGCATGGGCGACGGCAAGGACTGCCATCATCCGGCCTATCGCTTCAACGACAACATCCTTGGCC
ACGGCGCGTCGTACTGGGTGCGGCTGGTCGAGAAGACGATGCCGGCGGCGTGA

Upstream 100 bases:

>100_bases
ATGCAGCCATCGCCCGTTCAAAGCTTGTGTCCGCCGGTCGTTCCGGCCATGGTCCTGGCTCCCGCGTTGACCGAGAAGCC
TTGAACGAGAAGCCGCCACC

Downstream 100 bases:

>100_bases
GGCTCGGCCGGAACCCGGCGGCCGCCGGGTTTTGACGGAGGGAGGGAGTGATGGACCGCGCCGCCCGGGGATGCTATCCC
GCCACCCCACATGAGTGACC

Product: putative hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPILNSIAALTDDMAAWRHDFHEHPELMYEVHRTAGIVAERLREFGCDEVVTGIGRTGVVGVIRGRKTDSGRTIGLRADM
DALPIEEASGVPYASKTPGLMHACGHDGHTAMLLGAAKHLADTRNFDGTAIVIFQPAEEGGAGGKAMVDDGLMTRWGIQE
VYGLHNAPGVPEGVFALRPGPMLASSDQLEITVHGKGGHAGAGVHKAVDSVLIAAHIITALQSIVSRNVDPIKSAVISIC
MVEAGKAMNVIPETVELKGTVRTLDPEVQDLVERRIAEVADGIARTYGGTATTKYTRLYPITMNHARETGVAADVARDVV
GAERVVDNAPPLMGAEDFAFMLQARPGAFVFLGMGDGKDCHHPAYRFNDNILGHGASYWVRLVEKTMPAA

Sequences:

>Translated_390_residues
MPILNSIAALTDDMAAWRHDFHEHPELMYEVHRTAGIVAERLREFGCDEVVTGIGRTGVVGVIRGRKTDSGRTIGLRADM
DALPIEEASGVPYASKTPGLMHACGHDGHTAMLLGAAKHLADTRNFDGTAIVIFQPAEEGGAGGKAMVDDGLMTRWGIQE
VYGLHNAPGVPEGVFALRPGPMLASSDQLEITVHGKGGHAGAGVHKAVDSVLIAAHIITALQSIVSRNVDPIKSAVISIC
MVEAGKAMNVIPETVELKGTVRTLDPEVQDLVERRIAEVADGIARTYGGTATTKYTRLYPITMNHARETGVAADVARDVV
GAERVVDNAPPLMGAEDFAFMLQARPGAFVFLGMGDGKDCHHPAYRFNDNILGHGASYWVRLVEKTMPAA
>Mature_389_residues
PILNSIAALTDDMAAWRHDFHEHPELMYEVHRTAGIVAERLREFGCDEVVTGIGRTGVVGVIRGRKTDSGRTIGLRADMD
ALPIEEASGVPYASKTPGLMHACGHDGHTAMLLGAAKHLADTRNFDGTAIVIFQPAEEGGAGGKAMVDDGLMTRWGIQEV
YGLHNAPGVPEGVFALRPGPMLASSDQLEITVHGKGGHAGAGVHKAVDSVLIAAHIITALQSIVSRNVDPIKSAVISICM
VEAGKAMNVIPETVELKGTVRTLDPEVQDLVERRIAEVADGIARTYGGTATTKYTRLYPITMNHARETGVAADVARDVVG
AERVVDNAPPLMGAEDFAFMLQARPGAFVFLGMGDGKDCHHPAYRFNDNILGHGASYWVRLVEKTMPAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=422, Percent_Identity=27.7251184834123, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41550; Mature: 41419

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILNSIAALTDDMAAWRHDFHEHPELMYEVHRTAGIVAERLREFGCDEVVTGIGRTGVV
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRKTDSGRTIGLRADMDALPIEEASGVPYASKTPGLMHACGHDGHTAMLLGAAKHL
EEEECCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
ADTRNFDGTAIVIFQPAEEGGAGGKAMVDDGLMTRWGIQEVYGLHNAPGVPEGVFALRPG
HHCCCCCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCC
PMLASSDQLEITVHGKGGHAGAGVHKAVDSVLIAAHIITALQSIVSRNVDPIKSAVISIC
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MVEAGKAMNVIPETVELKGTVRTLDPEVQDLVERRIAEVADGIARTYGGTATTKYTRLYP
HHHCCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
ITMNHARETGVAADVARDVVGAERVVDNAPPLMGAEDFAFMLQARPGAFVFLGMGDGKDC
EEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCEEEEEECCCCCCC
HHPAYRFNDNILGHGASYWVRLVEKTMPAA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PILNSIAALTDDMAAWRHDFHEHPELMYEVHRTAGIVAERLREFGCDEVVTGIGRTGVV
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRKTDSGRTIGLRADMDALPIEEASGVPYASKTPGLMHACGHDGHTAMLLGAAKHL
EEEECCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
ADTRNFDGTAIVIFQPAEEGGAGGKAMVDDGLMTRWGIQEVYGLHNAPGVPEGVFALRPG
HHCCCCCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCC
PMLASSDQLEITVHGKGGHAGAGVHKAVDSVLIAAHIITALQSIVSRNVDPIKSAVISIC
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MVEAGKAMNVIPETVELKGTVRTLDPEVQDLVERRIAEVADGIARTYGGTATTKYTRLYP
HHHCCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
ITMNHARETGVAADVARDVVGAERVVDNAPPLMGAEDFAFMLQARPGAFVFLGMGDGKDC
EEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCEEEEEECCCCCCC
HHPAYRFNDNILGHGASYWVRLVEKTMPAA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]