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The map label for this gene is bam [H]

Identifier: 146338832

GI number: 146338832

Start: 1891908

End: 1893467

Strand: Reverse

Name: bam [H]

Synonym: BRADO1769

Alternate gene names: 146338832

Gene position: 1893467-1891908 (Counterclockwise)

Preceding gene: 146338833

Following gene: 146338831

Centisome position: 25.39

GC content: 68.91

Gene sequence:

>1560_bases
ATGGCAAAGACAGCGAAGAAGCCGGCCGCGAAGGCCGCCCGGCAGGTCCTGAAGCGTGCCGTGAACGCCAAGGCCAAGAC
GAAGCCCGTCGCCAAGAGCAAGAAATCACCTAAGACGGCGCCGACGACAGCCGCGCCCAAGCCGCGGCTGCCGAAGGGGC
CGGCCTGGCAATGGTCGGCGGTCGAGACCGCGGCGGCGATCCGCTCGGGCGCCGTCTCCTCGGTCGAGGTGACCCAGGCG
CACATCGCGCGCATGCACGAGGTCAATCCCAAGCTCAATGCGGTCGTCGTCGACCTCAGCGAGGAGGCCTTGAAAGCCGC
GCGGGCCGCCGACAAGGCGCGCGACAAGAGCGGCCTGCTGCACGGCGTGCCGGTCACGATCAAGGAGAACGTCGACTACG
AGGGCCGGCCGAACCCCAATGGCGTCAAGGCCCAGATGGGCATCATCGCGCCGTCGGACGCGCCGGTCGTGCGCAACCTC
AAGAAGGCAGGCGCCATCGTGCTCGGCCTGACCAACACGCCGGAATTCTCGTTTCGCGGCTTCACCGACAATCCGCTGCA
TGGGCTCACGCTGAATCCTTGGGATCCGGAGATCACCTGCGGCGGCTCGTCCGGCGGCGCCGGCGCCGCGGTCGCCGCCG
GCATCGGCACGATCGCGCATGGCAACGACATCGGCGGCTCGCTGCGCTGGCCGGCGCATTGCAACGGCGTGGTCACGATC
AAGCCGACGCAAGGACGCATCCCCGCCTACAATGAAAGCGCCGCGTCCGAGCGGCCGATGCTGGCGCATCTGATGTCGTC
GCAGGGGCCGCTGGCGCGCAGCGTGGCCGATGTCCGGCTGGCGCTGGAGGTGATGAGCCGGCGCGATCCGCGCGACCCCT
GGTGGGTGCCGGCGCCGCTCGAAGGCGAGAAACCGAAGCGGCCGGTCAAGGTCGCGCTCGCCAAGATTCCCGACGACATG
GACGTCGATCCCTCCGTGCGCGCCGCGCTGCGGCAGGCCGCTGACCATCTCGAGCGCTCCGGCTATCGCGTCAGCGAGGT
CGAGGTGCCCGACATCTCCCGCGTCTGGCAGACCTGGTGCGACGTCATCACCACGGAGGTGGTGGTGATGCAGGAGGCCA
AGATGCTGGCCGTCACATCGGAGGACTTCCACAAGGCGTGGGGCGGGATGCGGGCGAAGGCGGAGATCCTCGATCTCGCC
GGCTGGATGAACGCGACGGCGCAGCGCTCGACGCATATCCGCGCGTGGCAGCTGTTCTTCGAGGACTATCCGGTCGTGCT
GGCGCCGACGACGGTGAAGCCGACGCCGGGTCCGCGCGACGACACCGTCAGCCCGGAGCGCGTGCAGGAGATCTTCTTCA
ACGAGATCCGCTTCATCTCCGCGATCAACGTGCTCGGCCTGCCGGGCGCCGTCGTCCCAGTCGCCGTTCACGATGGCCAT
CCGATCGGCGTGCAGCTGATCGCCGGCCGCTACCGCGAGGACCTCGCGCTCGATGCCGCCGCGGCGATCGAGAAGCGGGC
AGGGATGCTGGTCAAGCAGCTCTGGCAGAAGATCGGCTAG

Upstream 100 bases:

>100_bases
AGCGAACGACTCGCACGCCCTATGTCAGCCCGCTTCCCGCCGAGGTCGAGCCGCCGCTGACGATGTCGGTCGCCTGAGAC
GGAAACTGGGGGGAAACATA

Downstream 100 bases:

>100_bases
GCGACCCGGTCCCGGGTCCTGTTGGTGGCCACGAAGACAATTGACCCTCGCTGTTATCGTCGGTCAAGCCGGGCGTTGGC
AGCGAGGGTATGGCGCTATC

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAKTAKKPAAKAARQVLKRAVNAKAKTKPVAKSKKSPKTAPTTAAPKPRLPKGPAWQWSAVETAAAIRSGAVSSVEVTQA
HIARMHEVNPKLNAVVVDLSEEALKAARAADKARDKSGLLHGVPVTIKENVDYEGRPNPNGVKAQMGIIAPSDAPVVRNL
KKAGAIVLGLTNTPEFSFRGFTDNPLHGLTLNPWDPEITCGGSSGGAGAAVAAGIGTIAHGNDIGGSLRWPAHCNGVVTI
KPTQGRIPAYNESAASERPMLAHLMSSQGPLARSVADVRLALEVMSRRDPRDPWWVPAPLEGEKPKRPVKVALAKIPDDM
DVDPSVRAALRQAADHLERSGYRVSEVEVPDISRVWQTWCDVITTEVVVMQEAKMLAVTSEDFHKAWGGMRAKAEILDLA
GWMNATAQRSTHIRAWQLFFEDYPVVLAPTTVKPTPGPRDDTVSPERVQEIFFNEIRFISAINVLGLPGAVVPVAVHDGH
PIGVQLIAGRYREDLALDAAAAIEKRAGMLVKQLWQKIG

Sequences:

>Translated_519_residues
MAKTAKKPAAKAARQVLKRAVNAKAKTKPVAKSKKSPKTAPTTAAPKPRLPKGPAWQWSAVETAAAIRSGAVSSVEVTQA
HIARMHEVNPKLNAVVVDLSEEALKAARAADKARDKSGLLHGVPVTIKENVDYEGRPNPNGVKAQMGIIAPSDAPVVRNL
KKAGAIVLGLTNTPEFSFRGFTDNPLHGLTLNPWDPEITCGGSSGGAGAAVAAGIGTIAHGNDIGGSLRWPAHCNGVVTI
KPTQGRIPAYNESAASERPMLAHLMSSQGPLARSVADVRLALEVMSRRDPRDPWWVPAPLEGEKPKRPVKVALAKIPDDM
DVDPSVRAALRQAADHLERSGYRVSEVEVPDISRVWQTWCDVITTEVVVMQEAKMLAVTSEDFHKAWGGMRAKAEILDLA
GWMNATAQRSTHIRAWQLFFEDYPVVLAPTTVKPTPGPRDDTVSPERVQEIFFNEIRFISAINVLGLPGAVVPVAVHDGH
PIGVQLIAGRYREDLALDAAAAIEKRAGMLVKQLWQKIG
>Mature_518_residues
AKTAKKPAAKAARQVLKRAVNAKAKTKPVAKSKKSPKTAPTTAAPKPRLPKGPAWQWSAVETAAAIRSGAVSSVEVTQAH
IARMHEVNPKLNAVVVDLSEEALKAARAADKARDKSGLLHGVPVTIKENVDYEGRPNPNGVKAQMGIIAPSDAPVVRNLK
KAGAIVLGLTNTPEFSFRGFTDNPLHGLTLNPWDPEITCGGSSGGAGAAVAAGIGTIAHGNDIGGSLRWPAHCNGVVTIK
PTQGRIPAYNESAASERPMLAHLMSSQGPLARSVADVRLALEVMSRRDPRDPWWVPAPLEGEKPKRPVKVALAKIPDDMD
VDPSVRAALRQAADHLERSGYRVSEVEVPDISRVWQTWCDVITTEVVVMQEAKMLAVTSEDFHKAWGGMRAKAEILDLAG
WMNATAQRSTHIRAWQLFFEDYPVVLAPTTVKPTPGPRDDTVSPERVQEIFFNEIRFISAINVLGLPGAVVPVAVHDGHP
IGVQLIAGRYREDLALDAAAAIEKRAGMLVKQLWQKIG

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=257, Percent_Identity=37.3540856031128, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=27.7669902912621, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI166795287, Length=275, Percent_Identity=32, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=299, Percent_Identity=31.1036789297659, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=307, Percent_Identity=31.2703583061889, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=313, Percent_Identity=30.6709265175719, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=310, Percent_Identity=27.0967741935484, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=335, Percent_Identity=28.955223880597, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556278, Length=335, Percent_Identity=28.955223880597, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538254, Length=377, Percent_Identity=26.2599469496021, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=209, Percent_Identity=32.5358851674641, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=231, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=271, Percent_Identity=29.8892988929889, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=27.9918864097363, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24648435, Length=501, Percent_Identity=26.5469061876248, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24648437, Length=501, Percent_Identity=26.5469061876248, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45550774, Length=501, Percent_Identity=26.5469061876248, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078093, Length=487, Percent_Identity=28.1314168377823, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652985, Length=509, Percent_Identity=28.0943025540275, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=509, Percent_Identity=28.0943025540275, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=509, Percent_Identity=28.0943025540275, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=509, Percent_Identity=28.0943025540275, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21356731, Length=496, Percent_Identity=27.4193548387097, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24648441, Length=431, Percent_Identity=25.0580046403712, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648439, Length=431, Percent_Identity=25.0580046403712, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=26.9617706237425, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 55740; Mature: 55608

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTAKKPAAKAARQVLKRAVNAKAKTKPVAKSKKSPKTAPTTAAPKPRLPKGPAWQWSA
CCCCCCCHHHHHHHHHHHHHHCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VETAAAIRSGAVSSVEVTQAHIARMHEVNPKLNAVVVDLSEEALKAARAADKARDKSGLL
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCE
HGVPVTIKENVDYEGRPNPNGVKAQMGIIAPSDAPVVRNLKKAGAIVLGLTNTPEFSFRG
ECCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCEECC
FTDNPLHGLTLNPWDPEITCGGSSGGAGAAVAAGIGTIAHGNDIGGSLRWPAHCNGVVTI
CCCCCCCCCEECCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
KPTQGRIPAYNESAASERPMLAHLMSSQGPLARSVADVRLALEVMSRRDPRDPWWVPAPL
ECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
EGEKPKRPVKVALAKIPDDMDVDPSVRAALRQAADHLERSGYRVSEVEVPDISRVWQTWC
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DVITTEVVVMQEAKMLAVTSEDFHKAWGGMRAKAEILDLAGWMNATAQRSTHIRAWQLFF
HHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEHH
EDYPVVLAPTTVKPTPGPRDDTVSPERVQEIFFNEIRFISAINVLGLPGAVVPVAVHDGH
CCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
PIGVQLIAGRYREDLALDAAAAIEKRAGMLVKQLWQKIG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKTAKKPAAKAARQVLKRAVNAKAKTKPVAKSKKSPKTAPTTAAPKPRLPKGPAWQWSA
CCCCCCHHHHHHHHHHHHHHCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VETAAAIRSGAVSSVEVTQAHIARMHEVNPKLNAVVVDLSEEALKAARAADKARDKSGLL
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCE
HGVPVTIKENVDYEGRPNPNGVKAQMGIIAPSDAPVVRNLKKAGAIVLGLTNTPEFSFRG
ECCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCEECC
FTDNPLHGLTLNPWDPEITCGGSSGGAGAAVAAGIGTIAHGNDIGGSLRWPAHCNGVVTI
CCCCCCCCCEECCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
KPTQGRIPAYNESAASERPMLAHLMSSQGPLARSVADVRLALEVMSRRDPRDPWWVPAPL
ECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
EGEKPKRPVKVALAKIPDDMDVDPSVRAALRQAADHLERSGYRVSEVEVPDISRVWQTWC
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DVITTEVVVMQEAKMLAVTSEDFHKAWGGMRAKAEILDLAGWMNATAQRSTHIRAWQLFF
HHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEHH
EDYPVVLAPTTVKPTPGPRDDTVSPERVQEIFFNEIRFISAINVLGLPGAVVPVAVHDGH
CCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
PIGVQLIAGRYREDLALDAAAAIEKRAGMLVKQLWQKIG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]