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The map label for this gene is rbsB [H]

Identifier: 146338475

GI number: 146338475

Start: 1486095

End: 1487042

Strand: Reverse

Name: rbsB [H]

Synonym: BRADO1392

Alternate gene names: 146338475

Gene position: 1487042-1486095 (Counterclockwise)

Preceding gene: 146338476

Following gene: 146338474

Centisome position: 19.94

GC content: 65.51

Gene sequence:

>948_bases
ATGTTGAAGACCATTTCGCTCGCCGGTGCCGCGCTGGCGCTTGTCATGAGCTCCGCGCCGTCCTCGGCCAAGGAGCTGAA
ATCGATCGGCGTCTCGCTCGGCTCGCTCGGCAATCCGTTCTTCGTCGCCCTGTCGAAGGGCGCCGAGTTCGAAGCCAAGA
AGACCAATCCCAACGTCAAGATCACCGCGGTCGGCTTCGAATACGATCTCGGCAAGCAGGTCACCCAGATCGACAATTTC
ATCGCCGCCGGCGTCGATATGATCCTGCTCAATCCCGGTGATCCCAAGGCGATCGGCCCGGCGATCAAGAAGGCACAGGC
GGCGGGCATTCTCGTCGTCGCGGTCGACACCGCGGCCGAGGGCGCCGATGCCACCGTCACCACCAACAACGTGCAGGCCG
GCGAGATCTCCTGCCAGTACATCGTCGACAAGCTCGGCGGCAAGGGCGACGTGATCATCCAGAACGGCCCGCAGGTGTCG
GCCGTGATTGACCGCGTCAATGGCTGCAAGAGCGTGTTCGCCAAGGCCCCGGGCATCAAGGTGCTGTCGAGCGACCAGGA
CGGCAAGGGCTCGCGCGAAGGCGGCCTCACCGTGGCGCAGGGCTACCTGACGCGCTACGCGAAGATCGATGCGATCTTCG
CGATCAACGATCCGCAGGCGATCGGCACCGATCTCGCCGCCCGTCAGCAGGGACGCACCGGCATCATCATCACCTCCGTC
GACGGCGCCCCGGACATCGAGGCCGCCCTGAAGGATCCGGCCACCCCGCAGATCCAGGCCTCCGCCTCGCAGGATCCGTT
CTTCATGGCGCGCCGCGCCGTGCAGATCGGCGTCGGCCTGCTCAACGGCCAGAAGCCGGCGTCCACCGTAGAGCTCCTGC
CGTCCAAGCTGATCACGCGCGACAACGTCGCCGAATACAAGGGCTGGACCTCGGAGCGGAAGGAGTAG

Upstream 100 bases:

>100_bases
GATCAACCGGCGGGCCGCTGATGGCGGCAAGCTTCAACGTTCTTCCACGGCCGCTGGTCCGCCGTGCGCAGGGATTTAAA
AAGGACCAGGGAGGAAACCA

Downstream 100 bases:

>100_bases
AGCGCGACCCTCCGCTCTGATCGACCTGCTCTGCAACGCCGCCCCTCCCGGGCGGCGTTTTGTCGTTTAGACTGCCTTCG
CCAAACGGCTCAAACAACAC

Product: sugar ABC transporter periplasmic sugar-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLKTISLAGAALALVMSSAPSSAKELKSIGVSLGSLGNPFFVALSKGAEFEAKKTNPNVKITAVGFEYDLGKQVTQIDNF
IAAGVDMILLNPGDPKAIGPAIKKAQAAGILVVAVDTAAEGADATVTTNNVQAGEISCQYIVDKLGGKGDVIIQNGPQVS
AVIDRVNGCKSVFAKAPGIKVLSSDQDGKGSREGGLTVAQGYLTRYAKIDAIFAINDPQAIGTDLAARQQGRTGIIITSV
DGAPDIEAALKDPATPQIQASASQDPFFMARRAVQIGVGLLNGQKPASTVELLPSKLITRDNVAEYKGWTSERKE

Sequences:

>Translated_315_residues
MLKTISLAGAALALVMSSAPSSAKELKSIGVSLGSLGNPFFVALSKGAEFEAKKTNPNVKITAVGFEYDLGKQVTQIDNF
IAAGVDMILLNPGDPKAIGPAIKKAQAAGILVVAVDTAAEGADATVTTNNVQAGEISCQYIVDKLGGKGDVIIQNGPQVS
AVIDRVNGCKSVFAKAPGIKVLSSDQDGKGSREGGLTVAQGYLTRYAKIDAIFAINDPQAIGTDLAARQQGRTGIIITSV
DGAPDIEAALKDPATPQIQASASQDPFFMARRAVQIGVGLLNGQKPASTVELLPSKLITRDNVAEYKGWTSERKE
>Mature_315_residues
MLKTISLAGAALALVMSSAPSSAKELKSIGVSLGSLGNPFFVALSKGAEFEAKKTNPNVKITAVGFEYDLGKQVTQIDNF
IAAGVDMILLNPGDPKAIGPAIKKAQAAGILVVAVDTAAEGADATVTTNNVQAGEISCQYIVDKLGGKGDVIIQNGPQVS
AVIDRVNGCKSVFAKAPGIKVLSSDQDGKGSREGGLTVAQGYLTRYAKIDAIFAINDPQAIGTDLAARQQGRTGIIITSV
DGAPDIEAALKDPATPQIQASASQDPFFMARRAVQIGVGLLNGQKPASTVELLPSKLITRDNVAEYKGWTSERKE

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=288, Percent_Identity=30.9027777777778, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790526, Length=272, Percent_Identity=31.25, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790674, Length=193, Percent_Identity=30.0518134715026, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32623; Mature: 32623

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTISLAGAALALVMSSAPSSAKELKSIGVSLGSLGNPFFVALSKGAEFEAKKTNPNVK
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCEE
ITAVGFEYDLGKQVTQIDNFIAAGVDMILLNPGDPKAIGPAIKKAQAAGILVVAVDTAAE
EEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHCHHHHHHHHCCEEEEEEECCCC
GADATVTTNNVQAGEISCQYIVDKLGGKGDVIIQNGPQVSAVIDRVNGCKSVFAKAPGIK
CCCCEEEECCEECCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCE
VLSSDQDGKGSREGGLTVAQGYLTRYAKIDAIFAINDPQAIGTDLAARQQGRTGIIITSV
EEECCCCCCCCCCCCEEEHHHHHHHHEEEEEEEEECCCHHHCCHHHHHHCCCCEEEEEEC
DGAPDIEAALKDPATPQIQASASQDPFFMARRAVQIGVGLLNGQKPASTVELLPSKLITR
CCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHCC
DNVAEYKGWTSERKE
CCHHHHCCCCCCCCC
>Mature Secondary Structure
MLKTISLAGAALALVMSSAPSSAKELKSIGVSLGSLGNPFFVALSKGAEFEAKKTNPNVK
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCEE
ITAVGFEYDLGKQVTQIDNFIAAGVDMILLNPGDPKAIGPAIKKAQAAGILVVAVDTAAE
EEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHCHHHHHHHHCCEEEEEEECCCC
GADATVTTNNVQAGEISCQYIVDKLGGKGDVIIQNGPQVSAVIDRVNGCKSVFAKAPGIK
CCCCEEEECCEECCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCE
VLSSDQDGKGSREGGLTVAQGYLTRYAKIDAIFAINDPQAIGTDLAARQQGRTGIIITSV
EEECCCCCCCCCCCCEEEHHHHHHHHEEEEEEEEECCCHHHCCHHHHHHCCCCEEEEEEC
DGAPDIEAALKDPATPQIQASASQDPFFMARRAVQIGVGLLNGQKPASTVELLPSKLITR
CCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHCC
DNVAEYKGWTSERKE
CCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]