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The map label for this gene is rpoH [H]

Identifier: 146338455

GI number: 146338455

Start: 1456134

End: 1457033

Strand: Reverse

Name: rpoH [H]

Synonym: BRADO1371

Alternate gene names: 146338455

Gene position: 1457033-1456134 (Counterclockwise)

Preceding gene: 146338456

Following gene: 146338452

Centisome position: 19.54

GC content: 64.89

Gene sequence:

>900_bases
ATGGCCCGTACAGCTACACTGCCGGTTCTCAACGGAGAATCCGGTCTTGCTCGCTACCTCTCCGAGATCCGCAAGTTCCC
GATGCTGGAACCGCAGGAGGAGTACATGCTCGCCAAGCGTTGGCGTGAGCATGACGACAGCGACGCAGCCCATAAACTCG
TGACCAGCCACCTCAGGCTCGTGGCCAAGATCGCCATGGGCTATCGCGGCTACGGCCTGCCGATATCCGAAGTCGTGTCG
GAAGGCAATGTCGGCCTGATGCAGGCGGTGAAGCGGTTCGAGCCCGAGAAGGGTTTTCGCCTCGCCACCTACGCCATGTG
GTGGATCAAGGCGTCGATACAAGAGTACATCCTGCGGTCGTGGTCGCTCGTGAAGATGGGCACCACCGCGAACCAGAAGA
AGCTGTTCTTCAACCTGCGCAAGGCGAAGAGCAAGATCTCGGCGCTGGATGAAGGCGATCTGCGTCCGGACCAGGTCAAG
CTGATCGCGAAGCGCCTCGGCGTGACCGATCAGGACGTGATCGACATGAACCGCCGCCTCGGCGGCGACGCCTCGCTGAA
CGCGCCGATCCGCGACGACGGCGAAGCGGGCGAATGGCAGGACTGGCTGGTCGATACCTCGCCCAACCAGGAAGCCATCA
TGGCCGAGCACGAGGAATACGACCACCGCCGGCAGGCGCTGAACGGCGCGATCGGCGTGTTGAATGCGCGCGAGCGCCGC
ATCTTCGAGGCGCGGCGGCTGGCCGACGAGCCGATGACGCTCGAGGATCTCGCGGCCGAGTTCGGCGTGTCGCGCGAGCG
CGTCCGCCAGATCGAGGTGCGCGCGTTCGAGAAGGTGCAGTCCGCCGTCAAGGGCGCCATCGCCAAGCAGGAGGCGGCGG
CGCTGGAGGCCGCGCACTAG

Upstream 100 bases:

>100_bases
GGTCGACGCATGGAACGTCGCAAACCGGTTGGCTTTAACCAAGTGACCGTTTTGCCTGGCCCGCCGCTATCGGGGGCCTG
AACCAACTGGAGGGCGCTCG

Downstream 100 bases:

>100_bases
ACGGCGCGGCTTGCTTGAAGGTGGTTTTTGGATGCGATCGGAGCCGGCGGAGACGCCGGCTTCTTGTTGAGGAAGGTTGT
TTGAGAACGGTTGAAGAGAG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MARTATLPVLNGESGLARYLSEIRKFPMLEPQEEYMLAKRWREHDDSDAAHKLVTSHLRLVAKIAMGYRGYGLPISEVVS
EGNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVK
LIAKRLGVTDQDVIDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEYDHRRQALNGAIGVLNARERR
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH

Sequences:

>Translated_299_residues
MARTATLPVLNGESGLARYLSEIRKFPMLEPQEEYMLAKRWREHDDSDAAHKLVTSHLRLVAKIAMGYRGYGLPISEVVS
EGNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVK
LIAKRLGVTDQDVIDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEYDHRRQALNGAIGVLNARERR
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH
>Mature_298_residues
ARTATLPVLNGESGLARYLSEIRKFPMLEPQEEYMLAKRWREHDDSDAAHKLVTSHLRLVAKIAMGYRGYGLPISEVVSE
GNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVKL
IAKRLGVTDQDVIDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEYDHRRQALNGAIGVLNARERRI
FEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=299, Percent_Identity=38.7959866220736, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=33.457249070632, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=30.3643724696356, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33818; Mature: 33687

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTATLPVLNGESGLARYLSEIRKFPMLEPQEEYMLAKRWREHDDSDAAHKLVTSHLRL
CCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVKLIAKRLGVTDQDVIDMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
GGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEYDHRRQALNGAIGVLNARERR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARTATLPVLNGESGLARYLSEIRKFPMLEPQEEYMLAKRWREHDDSDAAHKLVTSHLRL
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPEKGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVKLIAKRLGVTDQDVIDMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
GGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEYDHRRQALNGAIGVLNARERR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]