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The map label for this gene is carA [H]

Identifier: 146338422

GI number: 146338422

Start: 1425592

End: 1426788

Strand: Reverse

Name: carA [H]

Synonym: BRADO1337

Alternate gene names: 146338422

Gene position: 1426788-1425592 (Counterclockwise)

Preceding gene: 146338427

Following gene: 146338421

Centisome position: 19.13

GC content: 65.33

Gene sequence:

>1197_bases
ATGACACACATCGATACTGACGCTGCCTGGCCGGACCACAAACCGACCGCGCTCCTCGTGCTCGCCGATGGAACGGTGCT
GGAAGGCTACGGCCTCGGCGCCGAAGGCCACGCCGTGGGCGAGGTCTGCTTCAACACCGCGATGACCGGCTATGAGGAGA
TCCTGACCGATCCGTCCTATGCCGGACAACTCATCACCTTCACCTTCCCGCATATCGGCAACGTCGGCACCAACGACGAG
GATATCGAGACGGTGAACATGGCGGCGACGCCGGGCGCGCGCGGGGTGATCCTGCGCACCGCGATATCAGATCCGTCGAA
CTACCGCGCCACACGGCATCTCGACCAATGGCTGCGCGCCCGCGGCATCATCGGGCTCTCAGGGATCGACACGCGCGCGC
TGACCGCGCTGATCCGCTCCAAGGGCATGCCGAATGCCGTGATCGCCCATTCCAAGGACGGCACCTTCGATCTCGACGCC
TTGAAGAAGGAAGCGCGGGAGTGGCCGGGCCTCGAGGGCATGGACCTCGTGCCGATGGTCACCTCGACGCAGCGCTTCAA
CTGGGACGAAACTCCCTGGGTGTGGAACGAGGGTTTCGGCCAGCAGGCCAAGCCGGAGTTCAACGTCGTCGCCATCGACT
ACGGCATCAAGCGCAACATCCTGCGCCTGCTCGCCGGCGTCGGCTGCAAGGTGACGGTGGTGCCGGCGACGACCTCGGCC
GAGAACATCATGGCGATGAAGCCGGACGGCGTGTTCCTGTCCAACGGTCCCGGCGATCCCGCCGCGACCGGCAAGTATGC
CGTGCCGGTCATCCAGCAGGTGATCGCCTCGGGGACGCCGACCTTCGGCATCTGCCTCGGGCATCAGATGCTCGGCCTCG
CGGTCGGCGCCAAGACCAAGAAGATGCATCAGGGCCATCACGGCGCCAATCATCCGGTCAAGGACGAGACCACGGGCAAG
GTCGAGATTACCTCGATGAACCACGGTTTCGCGGTCGACCAGGACACGTTGCCGGCGGGCGCGACGCAGACCCACATCTC
GCTGTTCGACGGCTCCAACTGCGGCATTCAGCTCGAGGGCAAGCCGGTGTTCTCCGTGCAGTACCACCCGGAAGCCTCGC
CCGGTCCGCGCGACTCGCATTATCTGTTCCAGCGCTTCGCCGAGCTGATGCGCGAGAAGAAGCGCAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGAAATCCGGCCATTCTCGGCGCAAATTGACCGAAATCGCGTGTGAATCGGCCACTTCCAGCTTTGACGTCGGGCTCTCG
TCCCACTATGTAGGGGCCTC

Downstream 100 bases:

>100_bases
GGCCGGCAGACTGATCGACATCACATGCCCGGGTCGCTCCCGGGCATTTCTGCATCCGACCTTGTGATCACGACGCCGCC
GTTTCAGGCTCGCGCTGCGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTHIDTDAAWPDHKPTALLVLADGTVLEGYGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRTAISDPSNYRATRHLDQWLRARGIIGLSGIDTRALTALIRSKGMPNAVIAHSKDGTFDLDA
LKKEAREWPGLEGMDLVPMVTSTQRFNWDETPWVWNEGFGQQAKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSA
ENIMAMKPDGVFLSNGPGDPAATGKYAVPVIQQVIASGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVKDETTGK
VEITSMNHGFAVDQDTLPAGATQTHISLFDGSNCGIQLEGKPVFSVQYHPEASPGPRDSHYLFQRFAELMREKKRSAA

Sequences:

>Translated_398_residues
MTHIDTDAAWPDHKPTALLVLADGTVLEGYGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDE
DIETVNMAATPGARGVILRTAISDPSNYRATRHLDQWLRARGIIGLSGIDTRALTALIRSKGMPNAVIAHSKDGTFDLDA
LKKEAREWPGLEGMDLVPMVTSTQRFNWDETPWVWNEGFGQQAKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSA
ENIMAMKPDGVFLSNGPGDPAATGKYAVPVIQQVIASGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVKDETTGK
VEITSMNHGFAVDQDTLPAGATQTHISLFDGSNCGIQLEGKPVFSVQYHPEASPGPRDSHYLFQRFAELMREKKRSAA
>Mature_397_residues
THIDTDAAWPDHKPTALLVLADGTVLEGYGLGAEGHAVGEVCFNTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDED
IETVNMAATPGARGVILRTAISDPSNYRATRHLDQWLRARGIIGLSGIDTRALTALIRSKGMPNAVIAHSKDGTFDLDAL
KKEAREWPGLEGMDLVPMVTSTQRFNWDETPWVWNEGFGQQAKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSAE
NIMAMKPDGVFLSNGPGDPAATGKYAVPVIQQVIASGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVKDETTGKV
EITSMNHGFAVDQDTLPAGATQTHISLFDGSNCGIQLEGKPVFSVQYHPEASPGPRDSHYLFQRFAELMREKKRSAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=39.1644908616188, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI21361331, Length=390, Percent_Identity=36.4102564102564, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI169790915, Length=390, Percent_Identity=36.4102564102564, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1786215, Length=385, Percent_Identity=51.9480519480519, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI193204318, Length=387, Percent_Identity=39.2764857881137, Blast_Score=230, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=35.8778625954198, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=35.6020942408377, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=36.1538461538462, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=36.1538461538462, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42824; Mature: 42693

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIDTDAAWPDHKPTALLVLADGTVLEGYGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRTAISDPSNYRATRHLDQWLRA
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RGIIGLSGIDTRALTALIRSKGMPNAVIAHSKDGTFDLDALKKEAREWPGLEGMDLVPMV
CCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
TSTQRFNWDETPWVWNEGFGQQAKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSA
ECCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCCC
ENIMAMKPDGVFLSNGPGDPAATGKYAVPVIQQVIASGTPTFGICLGHQMLGLAVGAKTK
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHH
KMHQGHHGANHPVKDETTGKVEITSMNHGFAVDQDTLPAGATQTHISLFDGSNCGIQLEG
HHHCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECC
KPVFSVQYHPEASPGPRDSHYLFQRFAELMREKKRSAA
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THIDTDAAWPDHKPTALLVLADGTVLEGYGLGAEGHAVGEVCFNTAMTGYEEILTDPSY
CCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRTAISDPSNYRATRHLDQWLRA
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RGIIGLSGIDTRALTALIRSKGMPNAVIAHSKDGTFDLDALKKEAREWPGLEGMDLVPMV
CCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
TSTQRFNWDETPWVWNEGFGQQAKPEFNVVAIDYGIKRNILRLLAGVGCKVTVVPATTSA
ECCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCCC
ENIMAMKPDGVFLSNGPGDPAATGKYAVPVIQQVIASGTPTFGICLGHQMLGLAVGAKTK
CCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHH
KMHQGHHGANHPVKDETTGKVEITSMNHGFAVDQDTLPAGATQTHISLFDGSNCGIQLEG
HHHCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECC
KPVFSVQYHPEASPGPRDSHYLFQRFAELMREKKRSAA
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]