Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 146338409

GI number: 146338409

Start: 1412478

End: 1413044

Strand: Reverse

Name: greA [H]

Synonym: BRADO1324

Alternate gene names: 146338409

Gene position: 1413044-1412478 (Counterclockwise)

Preceding gene: 146338410

Following gene: 146338408

Centisome position: 18.95

GC content: 62.96

Gene sequence:

>567_bases
TTGAGCGGCTATGCTTTTGGGCCCCGAAGGTCCATTCTGGAGATGCTGATCGTGCGCGGCCTTGCTCGCACGACTGAAGG
ACGAGAAGAGATGGTTGAAAAGGTTCCCATGACTGCGGGCGGCTACGCCGCGCTGGAGAACGAGCTGAAGCAGCGACAGT
CGGTGGACCGCCCGCGCATCATCGAGCACATCGCCGAGGCGCGCTCGCATGGCGACCTGTCGGAAAACGCGGAATATCAC
GCCGCCAAGGAAGAGCAGTCGCATAATGAAGGCCGCATCGCAGAGCTTGAGGACAAGCTCGCGCGCGCCGACATCATCGA
CGTGGCCAAGCTCTCCGGCGACACGATCAAGTTCGGCGCGACCGTGACCCTGATCGACGAGGACACCGAGAAGAAGGCGG
TCTGGCAGATCGTCGGCGAGGTCGAGGCGGACGCCAAGAAGGGCAAGATCTCGATCACTTCGCCGCTGGCACGCGCGCTG
ATCGGCAAGAAGATGGGGACCACCGTGGAGGTCATGGCTCCGGGCGGCGCCAAGTCCTACGAGATCGCCAAAGTGGAGTG
GCGCTGA

Upstream 100 bases:

>100_bases
CGCTAAGCGATTGGCAATCATCGGAAATAAAGCGCTCTCGGCAGGAACTCGCCGCTCCGTTTGATGTTGGTCCGGCCTTT
GACGGGTCGCTATCATCGGC

Downstream 100 bases:

>100_bases
GCCCTCATTGTTGTTCTCGGGCGTCTGATCGAACAAGGGCTGCGCGAAAGCGCGGCCCTTTCTCCTTTGACGGGTCCCCC
GCCGCGCATCCCTCTCGAAC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSGYAFGPRRSILEMLIVRGLARTTEGREEMVEKVPMTAGGYAALENELKQRQSVDRPRIIEHIAEARSHGDLSENAEYH
AAKEEQSHNEGRIAELEDKLARADIIDVAKLSGDTIKFGATVTLIDEDTEKKAVWQIVGEVEADAKKGKISITSPLARAL
IGKKMGTTVEVMAPGGAKSYEIAKVEWR

Sequences:

>Translated_188_residues
MSGYAFGPRRSILEMLIVRGLARTTEGREEMVEKVPMTAGGYAALENELKQRQSVDRPRIIEHIAEARSHGDLSENAEYH
AAKEEQSHNEGRIAELEDKLARADIIDVAKLSGDTIKFGATVTLIDEDTEKKAVWQIVGEVEADAKKGKISITSPLARAL
IGKKMGTTVEVMAPGGAKSYEIAKVEWR
>Mature_187_residues
SGYAFGPRRSILEMLIVRGLARTTEGREEMVEKVPMTAGGYAALENELKQRQSVDRPRIIEHIAEARSHGDLSENAEYHA
AKEEQSHNEGRIAELEDKLARADIIDVAKLSGDTIKFGATVTLIDEDTEKKAVWQIVGEVEADAKKGKISITSPLARALI
GKKMGTTVEVMAPGGAKSYEIAKVEWR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=48.4076433121019, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=33.5483870967742, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20647; Mature: 20516

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYAFGPRRSILEMLIVRGLARTTEGREEMVEKVPMTAGGYAALENELKQRQSVDRPRI
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
IEHIAEARSHGDLSENAEYHAAKEEQSHNEGRIAELEDKLARADIIDVAKLSGDTIKFGA
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
TVTLIDEDTEKKAVWQIVGEVEADAKKGKISITSPLARALIGKKMGTTVEVMAPGGAKSY
EEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCE
EIAKVEWR
EEEEEECC
>Mature Secondary Structure 
SGYAFGPRRSILEMLIVRGLARTTEGREEMVEKVPMTAGGYAALENELKQRQSVDRPRI
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
IEHIAEARSHGDLSENAEYHAAKEEQSHNEGRIAELEDKLARADIIDVAKLSGDTIKFGA
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
TVTLIDEDTEKKAVWQIVGEVEADAKKGKISITSPLARALIGKKMGTTVEVMAPGGAKSY
EEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCE
EIAKVEWR
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA