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The map label for this gene is lepA [H]

Identifier: 146337850

GI number: 146337850

Start: 770957

End: 772810

Strand: Reverse

Name: lepA [H]

Synonym: BRADO0731

Alternate gene names: 146337850

Gene position: 772810-770957 (Counterclockwise)

Preceding gene: 146337852

Following gene: 146337848

Centisome position: 10.36

GC content: 63.92

Gene sequence:

>1854_bases
ATGGTTGCTGGTCAGGGTGCTGTGATGCTATTTGCGCGACCCATGACGACCGTCCCCATTCCCAACATCCGCAACTTCTC
CATCGTCGCCCATATCGACCATGGAAAGTCCACGCTCGCCGACCGCCTGATCCAGATGACCGGCGGCCTGTCGGACCGCG
AGATGGCGGGCAAGGAGCAGGTGCTCGATTCGATGGACATCGAGCGCGAGCGCGGCATCACCATCAAGGCGCAGACCGTG
CGCCTGCAATACCGCGCCAAGGACGGCAAGGATTACATCTTCAACCTGATGGACACGCCCGGCCACGTCGACTTCGCCTA
CGAGGTGTCGCGGTCGCTGGCGGCCTGCGAAGGCTCGCTGCTGGTGGTCGATGCCAGCCAGGGCGTCGAGGCCCAGACGC
TCGCCAACGTCTACCAGGCGCTCGACAACAACCACGAGATCGTCCCGGTCCTCAACAAGATCGACCTGCCGGCCGCCGAG
CCGGACAAGATCAAGCAGCAGATCGAGGACGTCATCGGCATCGACGCTTCCGACGCTGTCATGATCTCGGCCAAGACGGG
CCTCGGCATTCCCGATGTGCTGGAAGCCATCGTCACCCGCCTGCCGCCGCCGAAGGGCGACCGCGAGGCGACGCTGAAGG
CGCTGCTGGTCGACAGCTGGTACGACGTCTATCTCGGCGTCGTCGTGCTCGTGCGCATCGTCGACGGTGTGATGAAGAAG
GGCCAGCGCATCCGCATGATGGGCACCGGCGCGGCCTATGACGTCGAGCGCGTCGGCTTCTTCACGCCGAAGATGCAGCA
GGTCGACGAGCTCGGCCCCGGCGAGGTCGGCTTCATCACCGCGGCGATCAAGGAAGTCGCCGATACCCGCGTCGGCGACA
CCATCACCGACGACCGCAAGCCGGTGTCCGAGATGCTGCCGGGCTTCAAACCGGCGATTCCGGTCGTGTTCTGCGGCCTG
TTCCCGGTCGACGCCGACGATTTCGAAACGCTGCGCGCGGCGATGGGCAAGCTCCGGCTCAACGACGCCAGCTTCTCGTT
CGAGATGGAGACCTCCGCCGCGCTCGGCTTCGGCTTCCGCTGCGGCTTCCTCGGCCTGCTGCATCTGGAGATCATCCAGG
AGAGGCTGTCCCGCGAATTCGATCTCAACCTGATCGCGACCGCGCCATCCGTCATCTACAAGATGAAGCTGACCGACGGC
GAGGAGATCGAGATCCACAATCCGGTCGACATGCCGGACGTGGTCAAGATCGCCGAGATCCAGGAGCCGTGGATCGAGGC
CACCATCCTGACGCCGGACGAATATCTCGGCAGCGTGCTCAAGCTCTGCCAGGACCGCCGCGGCAATCAGAAGGAGCTCA
CCTATGTGGGCAGCCGCGCGATGGTCAAATACGATTTGCCGCTCAACGAGGTGGTGTTCGACTTCTACGACCGGCTGAAG
TCGGTCTCCAAGGGTTACGCCTCGTTCGACTATCATCTGACCGACTACAAGCCGGCCGACCTCGTGAAGATGCAGATCCT
CGTCAACAACGAGCCGGTCGACGCGCTGTCGATGCTGGTTCACCGGACCCGCGCCGAGGGCCGCGGCCGCGCCATGGTCG
AGAAGATGAAGGACCTGATCCCGCCGCACATGTTCCAGATCCCGATCCAGGCGGCGATCGGCGGCAAGGTGATCGCGCGC
GAGACCGTGCGCGCGCTGCGCAAGGATGTCACCGCGAAGTGCTACGGCGGCGACATCACGCGCAAGCGCAAGCTTCTGGA
GAAGCAGAAGGAAGGCAAGAAGAAGATGCGGCAGTTCGGCAAGGTCGACATCCCGCAGGAAGCCTTCATCGCCGCGCTCA
AGGTGGACAGCTGA

Upstream 100 bases:

>100_bases
GGGTGCGGCAGGGAGGTACTCGACATCAGCCCTCGGTAGCCTGTTTTCGCGCCTCGGGGAATCAGCTTGGCGTGAAGAAA
GGCCCTGGAAATTCAGCAAT

Downstream 100 bases:

>100_bases
GGCCGTCGGGCGGGGCGCGCTCGTTCAGATCTGCAAGGTCGACGCAGCCGGCGCTATCAGGAGGTCTTGCCCAGCGCCTG
ACGTTTCTGCGCGCGCACCT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MVAGQGAVMLFARPMTTVPIPNIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQVLDSMDIERERGITIKAQTV
RLQYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQALDNNHEIVPVLNKIDLPAAE
PDKIKQQIEDVIGIDASDAVMISAKTGLGIPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRIVDGVMKK
GQRIRMMGTGAAYDVERVGFFTPKMQQVDELGPGEVGFITAAIKEVADTRVGDTITDDRKPVSEMLPGFKPAIPVVFCGL
FPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLNLIATAPSVIYKMKLTDG
EEIEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGSRAMVKYDLPLNEVVFDFYDRLK
SVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRTRAEGRGRAMVEKMKDLIPPHMFQIPIQAAIGGKVIAR
ETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS

Sequences:

>Translated_617_residues
MVAGQGAVMLFARPMTTVPIPNIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQVLDSMDIERERGITIKAQTV
RLQYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQALDNNHEIVPVLNKIDLPAAE
PDKIKQQIEDVIGIDASDAVMISAKTGLGIPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRIVDGVMKK
GQRIRMMGTGAAYDVERVGFFTPKMQQVDELGPGEVGFITAAIKEVADTRVGDTITDDRKPVSEMLPGFKPAIPVVFCGL
FPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLNLIATAPSVIYKMKLTDG
EEIEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGSRAMVKYDLPLNEVVFDFYDRLK
SVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRTRAEGRGRAMVEKMKDLIPPHMFQIPIQAAIGGKVIAR
ETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS
>Mature_617_residues
MVAGQGAVMLFARPMTTVPIPNIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQVLDSMDIERERGITIKAQTV
RLQYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQALDNNHEIVPVLNKIDLPAAE
PDKIKQQIEDVIGIDASDAVMISAKTGLGIPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRIVDGVMKK
GQRIRMMGTGAAYDVERVGFFTPKMQQVDELGPGEVGFITAAIKEVADTRVGDTITDDRKPVSEMLPGFKPAIPVVFCGL
FPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFDLNLIATAPSVIYKMKLTDG
EEIEIHNPVDMPDVVKIAEIQEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGSRAMVKYDLPLNEVVFDFYDRLK
SVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRTRAEGRGRAMVEKMKDLIPPHMFQIPIQAAIGGKVIAR
ETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=612, Percent_Identity=49.8366013071895, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=49.2537313432836, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI18390331, Length=164, Percent_Identity=37.8048780487805, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40.6896551724138, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=45.945945945946, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=45.945945945946, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=45.945945945946, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI25306283, Length=138, Percent_Identity=46.3768115942029, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=138, Percent_Identity=46.3768115942029, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=138, Percent_Identity=46.3768115942029, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=40.2061855670103, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=59.3959731543624, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=525, Percent_Identity=28.3809523809524, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=36.4197530864198, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=162, Percent_Identity=31.4814814814815, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789559, Length=225, Percent_Identity=29.3333333333333, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.1305057096248, Blast_Score=472, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=40.2877697841727, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=40.2877697841727, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=466, Percent_Identity=26.3948497854077, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=149, Percent_Identity=38.9261744966443, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.9426751592357, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=27.5555555555556, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=606, Percent_Identity=46.2046204620462, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=41.4965986394558, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=41.4965986394558, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=37.5661375661376, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324166, Length=142, Percent_Identity=40.1408450704225, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=37.6068376068376, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78706572, Length=611, Percent_Identity=45.3355155482815, Blast_Score=562, Evalue=1e-160,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=45.985401459854, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=38.6503067484663, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=37.5838926174497, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=37.5838926174497, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=37.5838926174497, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=33.9506172839506, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28572034, Length=271, Percent_Identity=26.9372693726937, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68422; Mature: 68422

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAGQGAVMLFARPMTTVPIPNIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQ
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCHHH
VLDSMDIERERGITIKAQTVRLQYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSL
HHHHHCCHHHCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCE
LVVDASQGVEAQTLANVYQALDNNHEIVPVLNKIDLPAAEPDKIKQQIEDVIGIDASDAV
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEE
MISAKTGLGIPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRIVDGVMKK
EEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GQRIRMMGTGAAYDVERVGFFTPKMQQVDELGPGEVGFITAAIKEVADTRVGDTITDDRK
CCEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PVSEMLPGFKPAIPVVFCGLFPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFR
CHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCHHHCCCHH
CGFLGLLHLEIIQERLSREFDLNLIATAPSVIYKMKLTDGEEIEIHNPVDMPDVVKIAEI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCHHHHHHHH
QEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGSRAMVKYDLPLNEVVFDFYDRLK
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
SVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRTRAEGRGRAMVEKMKDLI
HHHCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFG
CCCEEEEEHHHHCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
KVDIPQEAFIAALKVDS
CCCCCHHHHEEEEEECC
>Mature Secondary Structure
MVAGQGAVMLFARPMTTVPIPNIRNFSIVAHIDHGKSTLADRLIQMTGGLSDREMAGKEQ
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCHHH
VLDSMDIERERGITIKAQTVRLQYRAKDGKDYIFNLMDTPGHVDFAYEVSRSLAACEGSL
HHHHHCCHHHCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCE
LVVDASQGVEAQTLANVYQALDNNHEIVPVLNKIDLPAAEPDKIKQQIEDVIGIDASDAV
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEE
MISAKTGLGIPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRIVDGVMKK
EEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GQRIRMMGTGAAYDVERVGFFTPKMQQVDELGPGEVGFITAAIKEVADTRVGDTITDDRK
CCEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PVSEMLPGFKPAIPVVFCGLFPVDADDFETLRAAMGKLRLNDASFSFEMETSAALGFGFR
CHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCHHHCCCHH
CGFLGLLHLEIIQERLSREFDLNLIATAPSVIYKMKLTDGEEIEIHNPVDMPDVVKIAEI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCHHHHHHHH
QEPWIEATILTPDEYLGSVLKLCQDRRGNQKELTYVGSRAMVKYDLPLNEVVFDFYDRLK
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHH
SVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRTRAEGRGRAMVEKMKDLI
HHHCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFG
CCCEEEEEHHHHCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
KVDIPQEAFIAALKVDS
CCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA