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The map label for this gene is bclA [H]

Identifier: 146337807

GI number: 146337807

Start: 718992

End: 720605

Strand: Reverse

Name: bclA [H]

Synonym: BRADO0686

Alternate gene names: 146337807

Gene position: 720605-718992 (Counterclockwise)

Preceding gene: 146337808

Following gene: 146337804

Centisome position: 9.66

GC content: 64.37

Gene sequence:

>1614_bases
ATGACACGAGAGATTGCGGATCAGGTTCCTTCCGACAGCCCCGGCGCCCGCGAGATCGGCTTCGCCATCCCTGCACGCTA
CAACGCCAGCCGCGTGCTGTTCGACAATCTCGCCAAGGGCAATGCAAGCAAACCCGCGCTGATCGGCTCAGCCGGCGAGC
GCAGCTATGCCGAGCTATGCGTCGATGCTTGCCGCTGGGGCAACGGCCTGGCATCGCTCGGCCTCACCCGCGGCGATCGC
GTGCTGCTGTTCCTCGACGATACTCCGGCCTATCCGGCCGCGTTCTTCGGCGCAGTGCGCGCGGGCTACGTGCCGCTGCT
GATCAACACGCTGACGCCGCCCGACCTCTTGCAGTTCTACCTCGCGGATTCCGGCGCCAAGGTCGCTGTCGCCGACGCGG
AATTTGCCGGGCGGTTCGACTCCCAAGCCTGCGCGGAGACGGCGCTGCAGACCCTGATCGTCGTCAATGGCGTGGCGCCG
GCAACGGCGGCGCCGTCGACAATCGCGGGCGATCGCTGGCTGGCCGACTTCGCAGCTGAGCTGGCCGAAGCCGACACGCA
TCGCAACGAGATGGCGTTCTGGATGTACTCGTCGGGCTCGACCGGGCGGCCGAAAGGCATCGTCCATCTGCAGCATGACA
TGGCCTATAGCGAGCAGGCCTTCGCGAGAAACGTGCTGCGGCTGAGAGCTGACGACATCTGCTTTTCGGTGCCGAAGATC
TTCTTCGCTTATGGGTTCGGCAATTCCATCACCTTTCCATTCACCGCCGGCGCGACAACGTTGCTGCTGCCCGGTCAACC
GAAGCCCGCGGCCATCTTCGATGCGATCGGGCGCTTTCGGCCGACCGTGTTCTTCGGCCTGCCGACGCTCTACACCGCCC
TTACCAAGGCAGACCGCGCCGTCGAAGCCGACTTGTCCTCGTTGCGTCTGTCGGTCTCCGCGGCCGAGGTGCTCTCGGCG
GAGGTCTTCAACGGCTGGAAAAGTCTTACGGGGCTGGAGATCGTCGAAGGGCTTGGCTCGACCGAGGTGCTGCACGTCTA
TCTCTCCAACCGCGAGGACCGGAAGAAGCTCGGCGCAGCCGGGCTGCGTGTCCCCGGTTACGAGATCGCACTGCGGAATG
GGGAAGGGCGCGAGGTCGGGACTGGGGAGGAAGGCATTCTCTGGGTCCGTGGCGATTCAAATACGCCACTGTATTGGAAC
CGGCCGGACAAGACCGCGGAGACGATCCGAGAGGGCGGCTGGATCTATACCGGCGACCGGTTCGTCCGCGATGCCGATGG
CTTCCACTTCTTTCGCGGCAGGGCCGACGACCTCGTCAAGATCTCCGGACAATGGGTCTATCCGCTCGAGGTCGAGCTCT
GCCTTGCCGAGCACCCGGTCATCCGGGAATGCGCCGTGTTCGCGCATGAGCTGCCGGACCGGCGGATGACCCTCAAAGCC
GTCGTGGTTACCAATGGCGGCTCGCACGATGAGCCGGCCATGACCAAGGCGTTGCAGGACTTCGTCAAGGCCAAGCTGCT
GCCTTACAAATACCCACGGGAGATCGTCTTCATCGACGAACTGCCGAAGACCGGCACCGGCAAGATCGATCGCCAAGCGG
TGATGCGGCTATGA

Upstream 100 bases:

>100_bases
ACATCACGCGCTCGGGCCGGATCGACGACACCAGGCTACGCACCATGGCTCCGCAATTCATGGCCCGCTACGAGGCATCG
CGCGCCGGAGGAGGCCGGGC

Downstream 100 bases:

>100_bases
GCGAACGCGGTCCACCGCGCAGGCCCCAGCAACGGGTCTCAGCCGCCTTTGCCGAGATGCTCCATCGCGTCCTCGGCGCG
CAGCGGCACGCGCGACACCT

Product: putative AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTREIADQVPSDSPGAREIGFAIPARYNASRVLFDNLAKGNASKPALIGSAGERSYAELCVDACRWGNGLASLGLTRGDR
VLLFLDDTPAYPAAFFGAVRAGYVPLLINTLTPPDLLQFYLADSGAKVAVADAEFAGRFDSQACAETALQTLIVVNGVAP
ATAAPSTIAGDRWLADFAAELAEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEQAFARNVLRLRADDICFSVPKI
FFAYGFGNSITFPFTAGATTLLLPGQPKPAAIFDAIGRFRPTVFFGLPTLYTALTKADRAVEADLSSLRLSVSAAEVLSA
EVFNGWKSLTGLEIVEGLGSTEVLHVYLSNREDRKKLGAAGLRVPGYEIALRNGEGREVGTGEEGILWVRGDSNTPLYWN
RPDKTAETIREGGWIYTGDRFVRDADGFHFFRGRADDLVKISGQWVYPLEVELCLAEHPVIRECAVFAHELPDRRMTLKA
VVVTNGGSHDEPAMTKALQDFVKAKLLPYKYPREIVFIDELPKTGTGKIDRQAVMRL

Sequences:

>Translated_537_residues
MTREIADQVPSDSPGAREIGFAIPARYNASRVLFDNLAKGNASKPALIGSAGERSYAELCVDACRWGNGLASLGLTRGDR
VLLFLDDTPAYPAAFFGAVRAGYVPLLINTLTPPDLLQFYLADSGAKVAVADAEFAGRFDSQACAETALQTLIVVNGVAP
ATAAPSTIAGDRWLADFAAELAEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEQAFARNVLRLRADDICFSVPKI
FFAYGFGNSITFPFTAGATTLLLPGQPKPAAIFDAIGRFRPTVFFGLPTLYTALTKADRAVEADLSSLRLSVSAAEVLSA
EVFNGWKSLTGLEIVEGLGSTEVLHVYLSNREDRKKLGAAGLRVPGYEIALRNGEGREVGTGEEGILWVRGDSNTPLYWN
RPDKTAETIREGGWIYTGDRFVRDADGFHFFRGRADDLVKISGQWVYPLEVELCLAEHPVIRECAVFAHELPDRRMTLKA
VVVTNGGSHDEPAMTKALQDFVKAKLLPYKYPREIVFIDELPKTGTGKIDRQAVMRL
>Mature_536_residues
TREIADQVPSDSPGAREIGFAIPARYNASRVLFDNLAKGNASKPALIGSAGERSYAELCVDACRWGNGLASLGLTRGDRV
LLFLDDTPAYPAAFFGAVRAGYVPLLINTLTPPDLLQFYLADSGAKVAVADAEFAGRFDSQACAETALQTLIVVNGVAPA
TAAPSTIAGDRWLADFAAELAEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEQAFARNVLRLRADDICFSVPKIF
FAYGFGNSITFPFTAGATTLLLPGQPKPAAIFDAIGRFRPTVFFGLPTLYTALTKADRAVEADLSSLRLSVSAAEVLSAE
VFNGWKSLTGLEIVEGLGSTEVLHVYLSNREDRKKLGAAGLRVPGYEIALRNGEGREVGTGEEGILWVRGDSNTPLYWNR
PDKTAETIREGGWIYTGDRFVRDADGFHFFRGRADDLVKISGQWVYPLEVELCLAEHPVIRECAVFAHELPDRRMTLKAV
VVTNGGSHDEPAMTKALQDFVKAKLLPYKYPREIVFIDELPKTGTGKIDRQAVMRL

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=547, Percent_Identity=30.3473491773309, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI58082049, Length=538, Percent_Identity=31.0408921933086, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI38505220, Length=507, Percent_Identity=31.3609467455621, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI157311624, Length=538, Percent_Identity=30.4832713754647, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI157311622, Length=538, Percent_Identity=30.4832713754647, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI122937307, Length=477, Percent_Identity=32.2851153039832, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI187761345, Length=588, Percent_Identity=27.3809523809524, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI187761343, Length=588, Percent_Identity=27.3809523809524, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI115511026, Length=483, Percent_Identity=28.3643892339545, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI28416953, Length=554, Percent_Identity=25.9927797833935, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI8923896, Length=542, Percent_Identity=25.6457564575646, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI253970489, Length=557, Percent_Identity=25.1346499102334, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI156151445, Length=518, Percent_Identity=22.972972972973, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI42544134, Length=383, Percent_Identity=25.5874673629243, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI195947378, Length=391, Percent_Identity=24.0409207161125, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI13375727, Length=561, Percent_Identity=22.4598930481283, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=28.698752228164, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790505, Length=563, Percent_Identity=26.1101243339254, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=25.0478011472275, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=26.0617760617761, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786529, Length=515, Percent_Identity=25.2427184466019, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789201, Length=495, Percent_Identity=26.4646464646465, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786801, Length=549, Percent_Identity=23.6794171220401, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=517, Percent_Identity=23.7911025145068, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=475, Percent_Identity=25.2631578947368, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=499, Percent_Identity=26.6533066132265, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=23.1827111984283, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=24.3761996161228, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=516, Percent_Identity=22.4806201550388, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=516, Percent_Identity=22.4806201550388, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=516, Percent_Identity=22.4806201550388, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=500, Percent_Identity=23.4, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=515, Percent_Identity=24.4660194174757, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=498, Percent_Identity=23.0923694779116, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=579, Percent_Identity=24.5250431778929, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=579, Percent_Identity=24.5250431778929, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=181, Percent_Identity=29.2817679558011, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560140, Length=479, Percent_Identity=22.964509394572, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323182, Length=578, Percent_Identity=24.0484429065744, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=574, Percent_Identity=25.609756097561, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319699, Length=352, Percent_Identity=24.7159090909091, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24581924, Length=509, Percent_Identity=25.7367387033399, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24667955, Length=527, Percent_Identity=26.9449715370019, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI62472339, Length=527, Percent_Identity=26.5654648956357, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI18859661, Length=480, Percent_Identity=27.5, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24667959, Length=490, Percent_Identity=26.734693877551, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355181, Length=366, Percent_Identity=27.8688524590164, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=23.4, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648260, Length=343, Percent_Identity=25.0728862973761, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648255, Length=483, Percent_Identity=22.9813664596273, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355225, Length=567, Percent_Identity=20.9876543209877, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648253, Length=483, Percent_Identity=22.5672877846791, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=22.4852071005917, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358303, Length=348, Percent_Identity=25.2873563218391, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20130357, Length=489, Percent_Identity=22.2903885480573, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=346, Percent_Identity=22.5433526011561, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356947, Length=543, Percent_Identity=22.6519337016575, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648257, Length=387, Percent_Identity=23.7726098191214, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58512; Mature: 58381

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREIADQVPSDSPGAREIGFAIPARYNASRVLFDNLAKGNASKPALIGSAGERSYAELC
CCCHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
VDACRWGNGLASLGLTRGDRVLLFLDDTPAYPAAFFGAVRAGYVPLLINTLTPPDLLQFY
HHHHHCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHH
LADSGAKVAVADAEFAGRFDSQACAETALQTLIVVNGVAPATAAPSTIAGDRWLADFAAE
HCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
LAEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEQAFARNVLRLRADDICFSVPKI
HHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAYGFGNSITFPFTAGATTLLLPGQPKPAAIFDAIGRFRPTVFFGLPTLYTALTKADRA
HEEECCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHH
VEADLSSLRLSVSAAEVLSAEVFNGWKSLTGLEIVEGLGSTEVLHVYLSNREDRKKLGAA
HHHHHHHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCHHHHHHCCC
GLRVPGYEIALRNGEGREVGTGEEGILWVRGDSNTPLYWNRPDKTAETIREGGWIYTGDR
CCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHCCCEEEECCE
FVRDADGFHFFRGRADDLVKISGQWVYPLEVELCLAEHPVIRECAVFAHELPDRRMTLKA
EEECCCCCEEECCCCCCEEEECCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
VVVTNGGSHDEPAMTKALQDFVKAKLLPYKYPREIVFIDELPKTGTGKIDRQAVMRL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
TREIADQVPSDSPGAREIGFAIPARYNASRVLFDNLAKGNASKPALIGSAGERSYAELC
CCHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
VDACRWGNGLASLGLTRGDRVLLFLDDTPAYPAAFFGAVRAGYVPLLINTLTPPDLLQFY
HHHHHCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHH
LADSGAKVAVADAEFAGRFDSQACAETALQTLIVVNGVAPATAAPSTIAGDRWLADFAAE
HCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
LAEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEQAFARNVLRLRADDICFSVPKI
HHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAYGFGNSITFPFTAGATTLLLPGQPKPAAIFDAIGRFRPTVFFGLPTLYTALTKADRA
HEEECCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHH
VEADLSSLRLSVSAAEVLSAEVFNGWKSLTGLEIVEGLGSTEVLHVYLSNREDRKKLGAA
HHHHHHHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCHHHHHHCCC
GLRVPGYEIALRNGEGREVGTGEEGILWVRGDSNTPLYWNRPDKTAETIREGGWIYTGDR
CCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHCCCEEEECCE
FVRDADGFHFFRGRADDLVKISGQWVYPLEVELCLAEHPVIRECAVFAHELPDRRMTLKA
EEECCCCCEEECCCCCCEEEECCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
VVVTNGGSHDEPAMTKALQDFVKAKLLPYKYPREIVFIDELPKTGTGKIDRQAVMRL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]