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The map label for this gene is ispG

Identifier: 146337679

GI number: 146337679

Start: 559535

End: 560836

Strand: Direct

Name: ispG

Synonym: BRADO0546

Alternate gene names: 146337679

Gene position: 559535-560836 (Clockwise)

Preceding gene: 146337678

Following gene: 146337681

Centisome position: 7.5

GC content: 67.67

Gene sequence:

>1302_bases
ATGAACAAGCCCGAGACAGTGACCGAGAACTCTCTAGCGTCCGATGTCGCCGGACCCGCTCCCCGGCATCAGACCACCAA
AGTCATGGTCGGCAACGTCGCCGTGGGCGGCGGGGCGCCGATCGTGGTCCAGTCCATGACCAACACTGACACCGCCGATG
TCGACTCGACGGTGGCCCAGGTCGCAGCGCTGGCGCGCGCCGGGTCGGAGATGGTCCGCATCACGGTCGATCGCGACGAG
GCGGCGGCCGCCGTCCCGCACATCCGCGAGCAGCTCGACAAGCGCGGCATCACCACGCCCCTGATCGGCGACTTCCACTA
CATCGGCCACAAGCTGCTGACTGAATACCCGGCCTGCGCCGAGGCGCTGGCGAAGTACCGCATCAATCCCGGCAATGTCG
GCTTCAAGGACAAGCGCGACACTCAGTTCTCGACCATCATCGAGCTCGCCAACAAATACGGCAAGCCGGTGCGCATCGGC
GCCAACTGGGGCTCGCTCGACCAGGAGCTGCTGTCGAAGCTGATGGACGAGAACGCGGCGTCCGCGAACCCGCGTAATGC
GCGGGCCGTGATGCGCGAGGCGATGGTGCAGTCGGCGCTGCACTCGGCCGAGCGCGCCCAAGAGCTCGGCATGCCAAAGG
ACCGCATCATCCTCTCCGCCAAGGTCTCGGCGGTGCAGGACCTGATCGCGGTGTACCAGGATCTCGCCGCGCGCTCCGAC
TACGCCATCCATCTCGGCCTCACCGAGGCCGGCATGGGCTCGAAGGGCATCGTCGCCTCCTCGGCCGCGCTCGGCATCCT
CCTGCAGCAGGGCATCGGCGACACCATCCGCATCTCGCTGACCCCCGAGCCCGGCGGCGACCGCACCCTCGAGGTGCAGG
TCGCGCAGGAACTGCTGCAGACCATGGGCTTCCGCACCTTCGTGCCGCTGGTCGCGGCCTGCCCGGGCTGCGGCCGCACC
ACCTCGACCACGTTCCAGGAGCTGGCGCGCTCGATCCAGGATTTCATCCGCGACGAGATGCCGACCTGGAAGATCAAATA
TCCCGGCGTCGAGGCGCTCAACGTCGCGGTCATGGGCTGCATCGTCAACGGCCCCGGCGAATCCAAACACGCCAATATCG
GCATCTCGCTTCCCGGCACCGGCGAAGCCCCGGCCGCCCCCGTGTTCGTCGACGGCAAGAAGTTCCGCACCTTGCGCGGC
CCCACCATCGCCGCCGACTTCAAGGCCCTGGTCATCGACTACATCGACCAGCGCTACGGCAGCGGCGCCAAGGCGCCGGA
GAGCGTGACCGCCGCGGAGTAA

Upstream 100 bases:

>100_bases
GTGATCATTGGGCTTTTTCTCGTAAACCGCCCGAACCGCGCGGTCGCCGCCGCGACTGATCCATTGCTGACCGTCACCAA
AACCTGATATCCGAGCGCCC

Downstream 100 bases:

>100_bases
GGGCGGTCGAAGGGAGTTTTTTCGCAGACGCAAGGAGTGCGTCTGTGAAGAATGTCCGCGAGTAAATCTAGCTGGCTAGC
ACGGCACATCGCGCATCGAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNKPETVTENSLASDVAGPAPRHQTTKVMVGNVAVGGGAPIVVQSMTNTDTADVDSTVAQVAALARAGSEMVRITVDRDE
AAAAVPHIREQLDKRGITTPLIGDFHYIGHKLLTEYPACAEALAKYRINPGNVGFKDKRDTQFSTIIELANKYGKPVRIG
ANWGSLDQELLSKLMDENAASANPRNARAVMREAMVQSALHSAERAQELGMPKDRIILSAKVSAVQDLIAVYQDLAARSD
YAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRT
TSTTFQELARSIQDFIRDEMPTWKIKYPGVEALNVAVMGCIVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFRTLRG
PTIAADFKALVIDYIDQRYGSGAKAPESVTAAE

Sequences:

>Translated_433_residues
MNKPETVTENSLASDVAGPAPRHQTTKVMVGNVAVGGGAPIVVQSMTNTDTADVDSTVAQVAALARAGSEMVRITVDRDE
AAAAVPHIREQLDKRGITTPLIGDFHYIGHKLLTEYPACAEALAKYRINPGNVGFKDKRDTQFSTIIELANKYGKPVRIG
ANWGSLDQELLSKLMDENAASANPRNARAVMREAMVQSALHSAERAQELGMPKDRIILSAKVSAVQDLIAVYQDLAARSD
YAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRT
TSTTFQELARSIQDFIRDEMPTWKIKYPGVEALNVAVMGCIVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFRTLRG
PTIAADFKALVIDYIDQRYGSGAKAPESVTAAE
>Mature_433_residues
MNKPETVTENSLASDVAGPAPRHQTTKVMVGNVAVGGGAPIVVQSMTNTDTADVDSTVAQVAALARAGSEMVRITVDRDE
AAAAVPHIREQLDKRGITTPLIGDFHYIGHKLLTEYPACAEALAKYRINPGNVGFKDKRDTQFSTIIELANKYGKPVRIG
ANWGSLDQELLSKLMDENAASANPRNARAVMREAMVQSALHSAERAQELGMPKDRIILSAKVSAVQDLIAVYQDLAARSD
YAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRT
TSTTFQELARSIQDFIRDEMPTWKIKYPGVEALNVAVMGCIVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFRTLRG
PTIAADFKALVIDYIDQRYGSGAKAPESVTAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=382, Percent_Identity=33.2460732984293, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BRASO (A4YKQ8)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202727.1
- STRING:   A4YKQ8
- GeneID:   5115316
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0546
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   BSP376:BRADO0546-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 45990; Mature: 45990

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPETVTENSLASDVAGPAPRHQTTKVMVGNVAVGGGAPIVVQSMTNTDTADVDSTVAQ
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHH
VAALARAGSEMVRITVDRDEAAAAVPHIREQLDKRGITTPLIGDFHYIGHKLLTEYPACA
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHH
EALAKYRINPGNVGFKDKRDTQFSTIIELANKYGKPVRIGANWGSLDQELLSKLMDENAA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCC
SANPRNARAVMREAMVQSALHSAERAQELGMPKDRIILSAKVSAVQDLIAVYQDLAARSD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHCCCC
YAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQ
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHH
TMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPTWKIKYPGVEALNVAVMGC
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHEEEEEE
IVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFRTLRGPTIAADFKALVIDYIDQRYG
EECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCC
SGAKAPESVTAAE
CCCCCCCCCCCCC
>Mature Secondary Structure
MNKPETVTENSLASDVAGPAPRHQTTKVMVGNVAVGGGAPIVVQSMTNTDTADVDSTVAQ
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHH
VAALARAGSEMVRITVDRDEAAAAVPHIREQLDKRGITTPLIGDFHYIGHKLLTEYPACA
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHH
EALAKYRINPGNVGFKDKRDTQFSTIIELANKYGKPVRIGANWGSLDQELLSKLMDENAA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCC
SANPRNARAVMREAMVQSALHSAERAQELGMPKDRIILSAKVSAVQDLIAVYQDLAARSD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHCCCC
YAIHLGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQ
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHH
TMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPTWKIKYPGVEALNVAVMGC
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHEEEEEE
IVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFRTLRGPTIAADFKALVIDYIDQRYG
EECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCC
SGAKAPESVTAAE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA