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The map label for this gene is proB [H]

Identifier: 146337576

GI number: 146337576

Start: 442014

End: 443150

Strand: Reverse

Name: proB [H]

Synonym: BRADO0431

Alternate gene names: 146337576

Gene position: 443150-442014 (Counterclockwise)

Preceding gene: 146337577

Following gene: 146337575

Centisome position: 5.94

GC content: 70.27

Gene sequence:

>1137_bases
ATGGCCCCCCGTCTCGAAAACTTCCGCCGCATCGTCGTCAAGGTCGGCTCGTCTCTGCTGGTCGATTCCGAGGCCGGCGA
GGTGCGCGCCGCCTGGTTGACCGCGCTGGCCGCCGACATCGCTCGGCTGCACGGCCGCGGCTGCGAGCTGCTGGTGGTGT
CCTCCGGCTCGATCGCGCTCGGGCGCAGCCGGCTGAAGCTGCCGCGCGGGCCGCTGAAGCTGGAGGAGAGCCAGGCGGCG
GCCGCGGTCGGCCAGATCGCCCTGGCGCGGATCTGGTCGGAAGTGCTGGGCGCGTACGGCATCGGCGCCGGCCAGATCCT
GGTCACCCTGCAGGACACCGAGGAGCGCCGCCGCTATCTCAACGCGCGCTCGACGATCGCAAAACTCCTCGAATGGCGCG
CAGTGCCGGTGATCAACGAGAACGACACGGTGGCGACCGCCGAAATCCGCTACGGCGACAATGACCGTCTCGCCGCCCGC
GTCGCGACCATGGCCAGCGCCGATTTGCTGGTGCTGCTGTCGGACATCGACGGCCTCTACACCGCTCCGCCCGCCCAGGA
TCCGAACGCGAAGCTGATCCCGGTGGTCGAGAGCATCACCGCCGATATCGAGGGCATCGCCGGCGGCGCGGCGTCGGAAT
TCTCCCGCGGCGGCATGAAGACCAAGATCGAAGCCGCGAAGATCGCGACCACCGGCGGCACCCACATGCTGATCGCCTCC
GGCAAGATCGAGCACCCGCTCGCGGCGATCGCCAATGGCGGCCGCTGCACCTGGTTTCTCACGCCGGCCAATCCGGTCAC
CTCGCGCAAACGCTGGATCGCCGGCTCGCTGGAGCCGAAGGGCACGCTGACGATCGATGCCGGCGCCGTTGCCGCGCTGC
GCGCCGGCAAGAGCCTGCTGCCGGCCGGCGTGATCAAGGTCGACGGCCAGTTCGCCCGCGGCGATGCCGTGGTGGTGCGC
GGCCCCGACACCGACGAGATCGGCCGCGGTCTGGTCGCCTACGACGCCGACGATGCCGAACGCATCAAGGGCCGGTCGTC
GCCGGATGTCGCCGTCATTCTCGGCATGTCCGGCCGGGCCGAGATGATCCACCGCGATGACCTTGTGATCGGGCCGGCGG
GGGTGGGTCCGGCCTAA

Upstream 100 bases:

>100_bases
TTGCGAAAGTCGCCACTTCATCAACACGCCCCTCGTTTCGTGATGCTGCGCCACGCAGCTCCCTCTCGCCCACGAGGGCC
TGAACCGACCCCTTGTTGCC

Downstream 100 bases:

>100_bases
CGCCGCCTCCGCCGCCAGCCGTCGCAAACTGCGGCTTTCCGTGCTACTGGAGAGCCTTGCTTCAAGGTTGAAACGCCCAT
GACCGCCCCTCTCAAAGCCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MAPRLENFRRIVVKVGSSLLVDSEAGEVRAAWLTALAADIARLHGRGCELLVVSSGSIALGRSRLKLPRGPLKLEESQAA
AAVGQIALARIWSEVLGAYGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATAEIRYGDNDRLAAR
VATMASADLLVLLSDIDGLYTAPPAQDPNAKLIPVVESITADIEGIAGGAASEFSRGGMKTKIEAAKIATTGGTHMLIAS
GKIEHPLAAIANGGRCTWFLTPANPVTSRKRWIAGSLEPKGTLTIDAGAVAALRAGKSLLPAGVIKVDGQFARGDAVVVR
GPDTDEIGRGLVAYDADDAERIKGRSSPDVAVILGMSGRAEMIHRDDLVIGPAGVGPA

Sequences:

>Translated_378_residues
MAPRLENFRRIVVKVGSSLLVDSEAGEVRAAWLTALAADIARLHGRGCELLVVSSGSIALGRSRLKLPRGPLKLEESQAA
AAVGQIALARIWSEVLGAYGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATAEIRYGDNDRLAAR
VATMASADLLVLLSDIDGLYTAPPAQDPNAKLIPVVESITADIEGIAGGAASEFSRGGMKTKIEAAKIATTGGTHMLIAS
GKIEHPLAAIANGGRCTWFLTPANPVTSRKRWIAGSLEPKGTLTIDAGAVAALRAGKSLLPAGVIKVDGQFARGDAVVVR
GPDTDEIGRGLVAYDADDAERIKGRSSPDVAVILGMSGRAEMIHRDDLVIGPAGVGPA
>Mature_377_residues
APRLENFRRIVVKVGSSLLVDSEAGEVRAAWLTALAADIARLHGRGCELLVVSSGSIALGRSRLKLPRGPLKLEESQAAA
AVGQIALARIWSEVLGAYGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATAEIRYGDNDRLAARV
ATMASADLLVLLSDIDGLYTAPPAQDPNAKLIPVVESITADIEGIAGGAASEFSRGGMKTKIEAAKIATTGGTHMLIASG
KIEHPLAAIANGGRCTWFLTPANPVTSRKRWIAGSLEPKGTLTIDAGAVAALRAGKSLLPAGVIKVDGQFARGDAVVVRG
PDTDEIGRGLVAYDADDAERIKGRSSPDVAVILGMSGRAEMIHRDDLVIGPAGVGPA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=318, Percent_Identity=32.3899371069182, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI21361368, Length=320, Percent_Identity=32.1875, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=40.8719346049046, Blast_Score=257, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=252, Percent_Identity=31.7460317460317, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=252, Percent_Identity=31.7460317460317, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=33.4134615384615, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321822, Length=415, Percent_Identity=32.0481927710843, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=31.2714776632302, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39537; Mature: 39406

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRLENFRRIVVKVGSSLLVDSEAGEVRAAWLTALAADIARLHGRGCELLVVSSGSIAL
CCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
GRSRLKLPRGPLKLEESQAAAAVGQIALARIWSEVLGAYGIGAGQILVTLQDTEERRRYL
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
NARSTIAKLLEWRAVPVINENDTVATAEIRYGDNDRLAARVATMASADLLVLLSDIDGLY
HHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
TAPPAQDPNAKLIPVVESITADIEGIAGGAASEFSRGGMKTKIEAAKIATTGGTHMLIAS
CCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCEEEEEC
GKIEHPLAAIANGGRCTWFLTPANPVTSRKRWIAGSLEPKGTLTIDAGAVAALRAGKSLL
CCCCCCHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCEEEECCCHHHHHHCCCCCC
PAGVIKVDGQFARGDAVVVRGPDTDEIGRGLVAYDADDAERIKGRSSPDVAVILGMSGRA
CCCEEEECCEEECCCEEEEECCCHHHHCCCEEEECCCCHHHHCCCCCCCEEEEEECCCCC
EMIHRDDLVIGPAGVGPA
CEEECCCEEECCCCCCCC
>Mature Secondary Structure 
APRLENFRRIVVKVGSSLLVDSEAGEVRAAWLTALAADIARLHGRGCELLVVSSGSIAL
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
GRSRLKLPRGPLKLEESQAAAAVGQIALARIWSEVLGAYGIGAGQILVTLQDTEERRRYL
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
NARSTIAKLLEWRAVPVINENDTVATAEIRYGDNDRLAARVATMASADLLVLLSDIDGLY
HHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
TAPPAQDPNAKLIPVVESITADIEGIAGGAASEFSRGGMKTKIEAAKIATTGGTHMLIAS
CCCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCEEEEEC
GKIEHPLAAIANGGRCTWFLTPANPVTSRKRWIAGSLEPKGTLTIDAGAVAALRAGKSLL
CCCCCCHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCEEEECCCHHHHHHCCCCCC
PAGVIKVDGQFARGDAVVVRGPDTDEIGRGLVAYDADDAERIKGRSSPDVAVILGMSGRA
CCCEEEECCEEECCCEEEEECCCHHHHCCCEEEECCCCHHHHCCCCCCCEEEEEECCCCC
EMIHRDDLVIGPAGVGPA
CEEECCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA