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The map label for this gene is trmD [H]

Identifier: 146337522

GI number: 146337522

Start: 381481

End: 382254

Strand: Reverse

Name: trmD [H]

Synonym: BRADO0376

Alternate gene names: 146337522

Gene position: 382254-381481 (Counterclockwise)

Preceding gene: 146337523

Following gene: 146337521

Centisome position: 5.13

GC content: 68.99

Gene sequence:

>774_bases
ATGACCACTTCGCCCCCTCTGACCGCGCCCTGGCGCGCGACTGTGCTGACGCTGTTTCCGGAGATGTTTCCCGGACCGCT
CGGCGTGAGCCTCGCCGGCCGGGCGCTGACATCAGGCATCTGGTCACTGGAGGCGCGCGACATCCGCGCGTCGGCCACCG
ACAAGCATCGCAGCGTCGATGACACGCCTGCCGGCGGCGGCCCGGGGATGGTGCTGCGCGCCGATGTGCTGGCAGCCGCC
ATCGACGCCGCGGCCATCGCACCCGAGCGGCCGAAGCTGCTGATGAGCCCGCGCGGCCGGCCATTGACCCAGGCCTTCGT
GCGCGAGCTCGCCGCGGGGCCCGGGCCGCTGATCGTCTGCGGCCGCTTCGAGGGCATTGACCAGCGCGTGATCGACGCAC
GCGAACTGATCGAGGTCTCGATCGGCGACTACGTGCTGTCGGGCGGCGAGATCGCCGCGCTGGTGCTGATCGACGCCTGC
GTGCGGCTACTGCCGGGCGTAATGGGAAAGCTCGAATCCGGCACCGACGAAAGCTTCTCCGACGGCCTACTCGAGTACCC
GCAATACACCCGACCGCAGGTCTTCGAGGGCCGTAGCATTCCGGATGTGCTGACCTCCGGCGATCACGCCAAGGTCGCGG
CGTGGCGCCATGCACAATCCCTGGCTCTCACCGAGGCGCGCAGGCCCGACCTCTGGGGCGAATGGCAGGCACGCCAGCCC
CGGGAACAAAGCAGGACCAGCCAAAAAGCGCCAAAAAGCACGACAGACGGGTGA

Upstream 100 bases:

>100_bases
GGCTTGTCCGGGACGACACCGGTGGTGGAGTCGCACCAGTCTTTGCGCTTGGCGCATTCGGTGGGCTCGATCTTGCTTCG
CCGCTGCCGTACAGAGCGCC

Downstream 100 bases:

>100_bases
CAAGCCTCGCGCTTTGCCTTATAGGAGCGCCCAATTCCGCAAGTGACGGCAGGAACAAGCGCAGCCCGCGTGACCCGGCT
GGGCGCGCCGCCGATGGAGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTTSPPLTAPWRATVLTLFPEMFPGPLGVSLAGRALTSGIWSLEARDIRASATDKHRSVDDTPAGGGPGMVLRADVLAAA
IDAAAIAPERPKLLMSPRGRPLTQAFVRELAAGPGPLIVCGRFEGIDQRVIDARELIEVSIGDYVLSGGEIAALVLIDAC
VRLLPGVMGKLESGTDESFSDGLLEYPQYTRPQVFEGRSIPDVLTSGDHAKVAAWRHAQSLALTEARRPDLWGEWQARQP
REQSRTSQKAPKSTTDG

Sequences:

>Translated_257_residues
MTTSPPLTAPWRATVLTLFPEMFPGPLGVSLAGRALTSGIWSLEARDIRASATDKHRSVDDTPAGGGPGMVLRADVLAAA
IDAAAIAPERPKLLMSPRGRPLTQAFVRELAAGPGPLIVCGRFEGIDQRVIDARELIEVSIGDYVLSGGEIAALVLIDAC
VRLLPGVMGKLESGTDESFSDGLLEYPQYTRPQVFEGRSIPDVLTSGDHAKVAAWRHAQSLALTEARRPDLWGEWQARQP
REQSRTSQKAPKSTTDG
>Mature_256_residues
TTSPPLTAPWRATVLTLFPEMFPGPLGVSLAGRALTSGIWSLEARDIRASATDKHRSVDDTPAGGGPGMVLRADVLAAAI
DAAAIAPERPKLLMSPRGRPLTQAFVRELAAGPGPLIVCGRFEGIDQRVIDARELIEVSIGDYVLSGGEIAALVLIDACV
RLLPGVMGKLESGTDESFSDGLLEYPQYTRPQVFEGRSIPDVLTSGDHAKVAAWRHAQSLALTEARRPDLWGEWQARQPR
EQSRTSQKAPKSTTDG

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=47.4885844748858, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27529; Mature: 27397

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPPLTAPWRATVLTLFPEMFPGPLGVSLAGRALTSGIWSLEARDIRASATDKHRSVD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCC
DTPAGGGPGMVLRADVLAAAIDAAAIAPERPKLLMSPRGRPLTQAFVRELAAGPGPLIVC
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEE
GRFEGIDQRVIDARELIEVSIGDYVLSGGEIAALVLIDACVRLLPGVMGKLESGTDESFS
ECCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DGLLEYPQYTRPQVFEGRSIPDVLTSGDHAKVAAWRHAQSLALTEARRPDLWGEWQARQP
CCHHCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
REQSRTSQKAPKSTTDG
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTSPPLTAPWRATVLTLFPEMFPGPLGVSLAGRALTSGIWSLEARDIRASATDKHRSVD
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCC
DTPAGGGPGMVLRADVLAAAIDAAAIAPERPKLLMSPRGRPLTQAFVRELAAGPGPLIVC
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEE
GRFEGIDQRVIDARELIEVSIGDYVLSGGEIAALVLIDACVRLLPGVMGKLESGTDESFS
ECCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DGLLEYPQYTRPQVFEGRSIPDVLTSGDHAKVAAWRHAQSLALTEARRPDLWGEWQARQP
CCHHCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
REQSRTSQKAPKSTTDG
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA