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The map label for this gene is mcl2 [H]

Identifier: 146337510

GI number: 146337510

Start: 372456

End: 373334

Strand: Direct

Name: mcl2 [H]

Synonym: BRADO0361

Alternate gene names: 146337510

Gene position: 372456-373334 (Clockwise)

Preceding gene: 146337508

Following gene: 146337516

Centisome position: 4.99

GC content: 67.35

Gene sequence:

>879_bases
ATGATCCGCCCGCGCCGCAGTCTCCTGTTCATGCCCGGCTCCAATGCCCGCGCGCTGGAGAAGGGACGGAACCTGCCGGC
CGACGGCCTGATCCTGGATCTCGAGGATTCCGTGGCGCCGGACGTCAAGGCGGCCGCGCGCGAGCAGATCGCCAAGGCGG
TCGCCGCCGATGGTTACGGCAAGCGCGAGCTGCTGATCCGCATCAACAGCCTGGATACGCCCTGGTGGAGCGACGACGTC
GCGATGGCCGGACAGACCGCGACCGACGGCATCCTGGTTCCCAAAGTGTCGACCGTCGCGGACCTGCGCACGGTGAGCAA
CCGCCTTGCCGAGGTCGGCGCCGATCCGGCGCTGAAGGTGTGGGCGATGATCGAGACCGCACGCGCCGTACTCGACGCGG
ACCTGCTCGCTGCGACCGTGCATGAGGCGGGCTCACGGCTCGCCGGCTTCGTGTTCGGGCCGAACGACATCTCGCGCGAG
ACCCGCATCCGCATGGTACCGGGCCGCGCGACGATGCTGCCGATGATCAGCCATTGCGTCCTGGCGACGCGCGCGCACGG
GCTCGAGATCCTCGATGGTCCCTACAGCGATTTCGCCAACACCGACGGCTTCGCGCAGGAATGCATCCAGGCCCGCGATC
TCGGCTTCGACGGTAAGACGCTGATCCATCCGGGCCAGATCGATGCCTGCAATGCGATCTTCACGCCGCCCGCGGTCGAA
ATTGCCGAAGCGCGCAAGATCCTGGCCGCGTTCGAACTACCCGAAAATGCATCGCGCGGCGCGATCCAGATCGACGGCCG
CATGGTCGAGCGGCTGCATGCCGAGATGGCCAAGCGCACGATCGCGATCGCGGACGCGATCGCCGAGATGACGAAGTAG

Upstream 100 bases:

>100_bases
GGCCCTGCCTAAACACATGCGGCCTGCGGGAACAAGCCTTTCGGCCCGGCAACCGAAATGGTATCGCGGGCCGCCGCCAT
AAACCAAGGGAAACGCCGAA

Downstream 100 bases:

>100_bases
TCAGAAGGAGGACTCGCCGCGTCAGATCTGTCGAAGGCTCAGGATGTAGTTGACGAGGTCGCCGCGCTGCTCCGACGTGA
GGATGACGTTGGGCATCGTC

Product: putative citrate lyase beta chain

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIRPRRSLLFMPGSNARALEKGRNLPADGLILDLEDSVAPDVKAAAREQIAKAVAADGYGKRELLIRINSLDTPWWSDDV
AMAGQTATDGILVPKVSTVADLRTVSNRLAEVGADPALKVWAMIETARAVLDADLLAATVHEAGSRLAGFVFGPNDISRE
TRIRMVPGRATMLPMISHCVLATRAHGLEILDGPYSDFANTDGFAQECIQARDLGFDGKTLIHPGQIDACNAIFTPPAVE
IAEARKILAAFELPENASRGAIQIDGRMVERLHAEMAKRTIAIADAIAEMTK

Sequences:

>Translated_292_residues
MIRPRRSLLFMPGSNARALEKGRNLPADGLILDLEDSVAPDVKAAAREQIAKAVAADGYGKRELLIRINSLDTPWWSDDV
AMAGQTATDGILVPKVSTVADLRTVSNRLAEVGADPALKVWAMIETARAVLDADLLAATVHEAGSRLAGFVFGPNDISRE
TRIRMVPGRATMLPMISHCVLATRAHGLEILDGPYSDFANTDGFAQECIQARDLGFDGKTLIHPGQIDACNAIFTPPAVE
IAEARKILAAFELPENASRGAIQIDGRMVERLHAEMAKRTIAIADAIAEMTK
>Mature_292_residues
MIRPRRSLLFMPGSNARALEKGRNLPADGLILDLEDSVAPDVKAAAREQIAKAVAADGYGKRELLIRINSLDTPWWSDDV
AMAGQTATDGILVPKVSTVADLRTVSNRLAEVGADPALKVWAMIETARAVLDADLLAATVHEAGSRLAGFVFGPNDISRE
TRIRMVPGRATMLPMISHCVLATRAHGLEILDGPYSDFANTDGFAQECIQARDLGFDGKTLIHPGQIDACNAIFTPPAVE
IAEARKILAAFELPENASRGAIQIDGRMVERLHAEMAKRTIAIADAIAEMTK

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=296, Percent_Identity=25.6756756756757, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI87081764, Length=273, Percent_Identity=32.2344322344322, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17557458, Length=286, Percent_Identity=31.1188811188811, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31400; Mature: 31400

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPRRSLLFMPGSNARALEKGRNLPADGLILDLEDSVAPDVKAAAREQIAKAVAADGYG
CCCCCCCEEEECCCCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
KRELLIRINSLDTPWWSDDVAMAGQTATDGILVPKVSTVADLRTVSNRLAEVGADPALKV
CEEEEEEEECCCCCCCCCCHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
WAMIETARAVLDADLLAATVHEAGSRLAGFVFGPNDISRETRIRMVPGRATMLPMISHCV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
LATRAHGLEILDGPYSDFANTDGFAQECIQARDLGFDGKTLIHPGQIDACNAIFTPPAVE
HHHHHCCCEEECCCCHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECCCCHHH
IAEARKILAAFELPENASRGAIQIDGRMVERLHAEMAKRTIAIADAIAEMTK
HHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRPRRSLLFMPGSNARALEKGRNLPADGLILDLEDSVAPDVKAAAREQIAKAVAADGYG
CCCCCCCEEEECCCCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
KRELLIRINSLDTPWWSDDVAMAGQTATDGILVPKVSTVADLRTVSNRLAEVGADPALKV
CEEEEEEEECCCCCCCCCCHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
WAMIETARAVLDADLLAATVHEAGSRLAGFVFGPNDISRETRIRMVPGRATMLPMISHCV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
LATRAHGLEILDGPYSDFANTDGFAQECIQARDLGFDGKTLIHPGQIDACNAIFTPPAVE
HHHHHCCCEEECCCCHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEECCCCHHH
IAEARKILAAFELPENASRGAIQIDGRMVERLHAEMAKRTIAIADAIAEMTK
HHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA