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The map label for this gene is cynT [H]

Identifier: 146337509

GI number: 146337509

Start: 371710

End: 372354

Strand: Reverse

Name: cynT [H]

Synonym: BRADO0360

Alternate gene names: 146337509

Gene position: 372354-371710 (Counterclockwise)

Preceding gene: 146337511

Following gene: 146337506

Centisome position: 4.99

GC content: 64.34

Gene sequence:

>645_bases
ATGATCTCGTTTCCGCAACGTCTGCTCGAGGGCTATCGCGCCTTCACCACCCAGCGCCTGCCGACCGAACAGAGCCGCTA
TCTGGAGCTGTCCGAGCGCGGCCAGTCGCCGGAAGTGATGGTGATCGGCTGCTGCGATTCCCGCGTCTCGCCGGAGGTCA
TCTTCGACGTCGGCCCCGGCGAATTGTTCGTCCTGCGCAACATCGCCAATCTCGTGCCGATCTATCAGCCCGACGCCAAT
GCGCATGGCGTGTCGGCGGCATTGGAATATGCCGTCACCGTGCTCATGGTGAAGCACATCGTCATCCTCGGCCATGCCCA
ATGCGGCGGCATCCGCGCCTTCGTCGACAAGGCGGCCCCGCTGTCGCCCGGCGACTTCATCGGCCGCTGGATGTCGATGT
TCATCAAGCCCGGCGAAATCGTCGAGCAGCGCGACCGGGAGAGCTTTCAGGACTTCGTCACCCGGATCGAGAAGGCCGCG
GTGTTCCGCAGCCTGGAGAATCTGATGACCTTCCCGTTCGTGCGCAGCCGCGTCGAGGCCGGTGATCTGCAGCTGCACGG
CGCTTATTTCGGCGTCGCCGAGGGCTCGTTGTTCGTGCTCGACCAGGCGGCAAAGGAGTTCCGCAGCGCGCGAGCGAGCC
AATAG

Upstream 100 bases:

>100_bases
TCGGCGTTTCCCTTGGTTTATGGCGGCGGCCCGCGATACCATTTCGGTTGCCGGGCCGAAAGGCTTGTTCCCGCAGGCCG
CATGTGTTTAGGCAGGGCCC

Downstream 100 bases:

>100_bases
CGAGGTCGCGAATGGCGAATAGGGAGTAGCAAGTAGTTTGGATTTCAATTCGCTACTCGCCATTCGCAACTTCGCCCCTC
TTCAGTGATCGCTCGTCCAG

Product: putative carbonic anhydrase 2 (carbonate dehydratase 2)(cynT)

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MISFPQRLLEGYRAFTTQRLPTEQSRYLELSERGQSPEVMVIGCCDSRVSPEVIFDVGPGELFVLRNIANLVPIYQPDAN
AHGVSAALEYAVTVLMVKHIVILGHAQCGGIRAFVDKAAPLSPGDFIGRWMSMFIKPGEIVEQRDRESFQDFVTRIEKAA
VFRSLENLMTFPFVRSRVEAGDLQLHGAYFGVAEGSLFVLDQAAKEFRSARASQ

Sequences:

>Translated_214_residues
MISFPQRLLEGYRAFTTQRLPTEQSRYLELSERGQSPEVMVIGCCDSRVSPEVIFDVGPGELFVLRNIANLVPIYQPDAN
AHGVSAALEYAVTVLMVKHIVILGHAQCGGIRAFVDKAAPLSPGDFIGRWMSMFIKPGEIVEQRDRESFQDFVTRIEKAA
VFRSLENLMTFPFVRSRVEAGDLQLHGAYFGVAEGSLFVLDQAAKEFRSARASQ
>Mature_214_residues
MISFPQRLLEGYRAFTTQRLPTEQSRYLELSERGQSPEVMVIGCCDSRVSPEVIFDVGPGELFVLRNIANLVPIYQPDAN
AHGVSAALEYAVTVLMVKHIVILGHAQCGGIRAFVDKAAPLSPGDFIGRWMSMFIKPGEIVEQRDRESFQDFVTRIEKAA
VFRSLENLMTFPFVRSRVEAGDLQLHGAYFGVAEGSLFVLDQAAKEFRSARASQ

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=202, Percent_Identity=32.1782178217822, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1786318, Length=193, Percent_Identity=31.6062176165803, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=211, Percent_Identity=25.5924170616114, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23830; Mature: 23830

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFPQRLLEGYRAFTTQRLPTEQSRYLELSERGQSPEVMVIGCCDSRVSPEVIFDVGPG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
ELFVLRNIANLVPIYQPDANAHGVSAALEYAVTVLMVKHIVILGHAQCGGIRAFVDKAAP
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
LSPGDFIGRWMSMFIKPGEIVEQRDRESFQDFVTRIEKAAVFRSLENLMTFPFVRSRVEA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDLQLHGAYFGVAEGSLFVLDQAAKEFRSARASQ
CCEEEECEEEEECCCCEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISFPQRLLEGYRAFTTQRLPTEQSRYLELSERGQSPEVMVIGCCDSRVSPEVIFDVGPG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
ELFVLRNIANLVPIYQPDANAHGVSAALEYAVTVLMVKHIVILGHAQCGGIRAFVDKAAP
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
LSPGDFIGRWMSMFIKPGEIVEQRDRESFQDFVTRIEKAAVFRSLENLMTFPFVRSRVEA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDLQLHGAYFGVAEGSLFVLDQAAKEFRSARASQ
CCEEEECEEEEECCCCEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]