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The map label for this gene is purH [H]

Identifier: 146337492

GI number: 146337492

Start: 350840

End: 352432

Strand: Reverse

Name: purH [H]

Synonym: BRADO0338

Alternate gene names: 146337492

Gene position: 352432-350840 (Counterclockwise)

Preceding gene: 146337493

Following gene: 146337490

Centisome position: 4.73

GC content: 67.61

Gene sequence:

>1593_bases
ATGACCAATCAGCTTCGTCCCGTTCGTCGTGCTCTTCTCTCGGTTTCCGACAAGACCCGCCTGACCGACTTCGCGCGCGC
GCTCGCCGCGCGCGGCGTCGAATTGATCTCCACCGGCGGCACCGCCAAGGCGATTGCCGAGGCCGGCCTGAAGGTGAAGG
ACGTCTCCGACCTCACCGGCTTTCCCGAGATGATGGACGGCCGGGTCAAGACCTTGCACCCCAAGGTGCATGGCGGCCTG
CTCGCGATCCGCGACAATGACGAGCATACCGAGGCGATGAAGACCCACGGCATCGCACCGATCGATCTGCTCGTCGTGAA
TCTCTATCCGTTCGAGGCCACGGTCGAGCGCGGCGCTCAGTTCAGCGATTGTATCGAGAATATCGACATCGGCGGCCCTG
CGATGATCCGCGCGGCGTCCAAGAACCACGAGGACGTCGCCGTCGTCGTCGACGTCGACGACTACGAGGCCGTGCTCGAG
GATCTCGCGCGGCACGAGGGCTCGACCACCTTGCTGCTGCGCCGCCGGCTTGCCGCCAAGGCCTATGCCCGCACCGCCGC
CTACGACGCCGCGATCTCGAACTGGTTCGCCGCGACCATCCACAATGACGCGCCCGACTATCGCGCCTTCGGCGGCAAGC
TGATCCAGTCGCTGCGCTATGGCGAGAACCCGCATCAGCATGCCGCGTTCTACGCCCTTCCCGGCCGACGTCCCGGCGTC
GCCACTGCGCGGCAGGTGCAGGGCAAGGAGCTGTCCTACAACAATATCAACGACACCGATGCGGCCTATGAATGCATCGC
CGAGTTCGATCCGGCCCGCACCGCGGCCTGCGTGATCGTGAAGCACGCCAACCCCTGCGGCGTGGCCGAGGGGCCGGATC
TGATCTCCGCCTATCAGAAGGCGCTGGCCTGCGATTCGACCTCGGCGTTCGGCGGCATCATCGCGATGAACCGCAAGCTC
GACGCCGCCACCGCGCGCGCCATCACCGGCATCTTCACCGAGGTGATCATCGCGCCCGACGCAACCGAGGAGGCGATCGC
GGTGGTCGCGGCGCGCAAGAACCTGCGGCTGCTGCTGGCGGGCGCCCTGCCCGATCCGCGCGAGGCCGGCCTCACGGCCA
AGACCGTCGCCGGCGGCCTGCTGGTGCAGAGCCGCGACAACGCCGTGGTCGACGACATGACGTTGAAGGTCGTGACCAAG
CGCGCGCCGACCGACGCCGAGTTGCGCGACCTGCGCTTCGCCTTCCGCGTCGCCAAGCATGTGAAGTCGAACACCATCGT
CTACGCCAAGGATTCGGCGACCGTGGGCATCGGCGCGGGACAGATGAGCCGGGTCGATTCGGCGCGCATTGCCGCCCGCA
AGGCGCTCGATGCCGCCGCCGAGCTGAAGCTCGCCGAGCCGCTGACCAAGGGCTCGGTGGTCGCCTCCGACGCGTTTTTC
CCGTTCGCCGACGGCATGCTCGCCTGCATCGAGGCCGGCGCCACCGCGGTGATCCAGCCGGGCGGATCGGTGCGCGACGA
CGAGGTCATCAAGGCTGCCGACGAACACGGCATCGCGATGGTGCTGACCGGCGTGCGGCACTTCCGGCACTAA

Upstream 100 bases:

>100_bases
CGCGCGACTGGCGACTTTGGATGGAGCACCATCGGGGAGCGCGGGATCGACCGCCGTGCGCCTGGGCAACTGAACCTCCT
GCAACAGGATTCTGCCCGCC

Downstream 100 bases:

>100_bases
CGAGCTCGTCATTGCCGGGCTTGACCCGGCAATCCATCATCGAATGACTTGATCGAAGTGAGATGGATGCGCGGGTCAAG
CCCGCGCATGACGAGAGGAG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTNQLRPVRRALLSVSDKTRLTDFARALAARGVELISTGGTAKAIAEAGLKVKDVSDLTGFPEMMDGRVKTLHPKVHGGL
LAIRDNDEHTEAMKTHGIAPIDLLVVNLYPFEATVERGAQFSDCIENIDIGGPAMIRAASKNHEDVAVVVDVDDYEAVLE
DLARHEGSTTLLLRRRLAAKAYARTAAYDAAISNWFAATIHNDAPDYRAFGGKLIQSLRYGENPHQHAAFYALPGRRPGV
ATARQVQGKELSYNNINDTDAAYECIAEFDPARTAACVIVKHANPCGVAEGPDLISAYQKALACDSTSAFGGIIAMNRKL
DAATARAITGIFTEVIIAPDATEEAIAVVAARKNLRLLLAGALPDPREAGLTAKTVAGGLLVQSRDNAVVDDMTLKVVTK
RAPTDAELRDLRFAFRVAKHVKSNTIVYAKDSATVGIGAGQMSRVDSARIAARKALDAAAELKLAEPLTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADEHGIAMVLTGVRHFRH

Sequences:

>Translated_530_residues
MTNQLRPVRRALLSVSDKTRLTDFARALAARGVELISTGGTAKAIAEAGLKVKDVSDLTGFPEMMDGRVKTLHPKVHGGL
LAIRDNDEHTEAMKTHGIAPIDLLVVNLYPFEATVERGAQFSDCIENIDIGGPAMIRAASKNHEDVAVVVDVDDYEAVLE
DLARHEGSTTLLLRRRLAAKAYARTAAYDAAISNWFAATIHNDAPDYRAFGGKLIQSLRYGENPHQHAAFYALPGRRPGV
ATARQVQGKELSYNNINDTDAAYECIAEFDPARTAACVIVKHANPCGVAEGPDLISAYQKALACDSTSAFGGIIAMNRKL
DAATARAITGIFTEVIIAPDATEEAIAVVAARKNLRLLLAGALPDPREAGLTAKTVAGGLLVQSRDNAVVDDMTLKVVTK
RAPTDAELRDLRFAFRVAKHVKSNTIVYAKDSATVGIGAGQMSRVDSARIAARKALDAAAELKLAEPLTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADEHGIAMVLTGVRHFRH
>Mature_529_residues
TNQLRPVRRALLSVSDKTRLTDFARALAARGVELISTGGTAKAIAEAGLKVKDVSDLTGFPEMMDGRVKTLHPKVHGGLL
AIRDNDEHTEAMKTHGIAPIDLLVVNLYPFEATVERGAQFSDCIENIDIGGPAMIRAASKNHEDVAVVVDVDDYEAVLED
LARHEGSTTLLLRRRLAAKAYARTAAYDAAISNWFAATIHNDAPDYRAFGGKLIQSLRYGENPHQHAAFYALPGRRPGVA
TARQVQGKELSYNNINDTDAAYECIAEFDPARTAACVIVKHANPCGVAEGPDLISAYQKALACDSTSAFGGIIAMNRKLD
AATARAITGIFTEVIIAPDATEEAIAVVAARKNLRLLLAGALPDPREAGLTAKTVAGGLLVQSRDNAVVDDMTLKVVTKR
APTDAELRDLRFAFRVAKHVKSNTIVYAKDSATVGIGAGQMSRVDSARIAARKALDAAAELKLAEPLTKGSVVASDAFFP
FADGMLACIEAGATAVIQPGGSVRDDEVIKAADEHGIAMVLTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=38.2536382536383, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1790439, Length=542, Percent_Identity=58.3025830258303, Blast_Score=582, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=33.8308457711443, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI71985556, Length=409, Percent_Identity=24.9388753056235, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71985574, Length=332, Percent_Identity=28.0120481927711, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323056, Length=609, Percent_Identity=32.8407224958949, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=605, Percent_Identity=32.2314049586777, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649832, Length=601, Percent_Identity=35.4409317803661, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56450; Mature: 56319

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQLRPVRRALLSVSDKTRLTDFARALAARGVELISTGGTAKAIAEAGLKVKDVSDLTG
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCEEEECHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNDEHTEAMKTHGIAPIDLLVVNLYPFEATVERGAQ
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHCCCH
FSDCIENIDIGGPAMIRAASKNHEDVAVVVDVDDYEAVLEDLARHEGSTTLLLRRRLAAK
HHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AYARTAAYDAAISNWFAATIHNDAPDYRAFGGKLIQSLRYGENPHQHAAFYALPGRRPGV
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECIAEFDPARTAACVIVKHANPCGVAEGPDLISAYQK
CCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSTSAFGGIIAMNRKLDAATARAITGIFTEVIIAPDATEEAIAVVAARKNLRLLLA
HHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEE
GALPDPREAGLTAKTVAGGLLVQSRDNAVVDDMTLKVVTKRAPTDAELRDLRFAFRVAKH
ECCCCHHHCCCCHHHHHCCEEEECCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHHH
VKSNTIVYAKDSATVGIGAGQMSRVDSARIAARKALDAAAELKLAEPLTKGSVVASDAFF
HCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADEHGIAMVLTGVRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCEEHEEHHHHHHCC
>Mature Secondary Structure 
TNQLRPVRRALLSVSDKTRLTDFARALAARGVELISTGGTAKAIAEAGLKVKDVSDLTG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCEEEECHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNDEHTEAMKTHGIAPIDLLVVNLYPFEATVERGAQ
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHCCCH
FSDCIENIDIGGPAMIRAASKNHEDVAVVVDVDDYEAVLEDLARHEGSTTLLLRRRLAAK
HHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AYARTAAYDAAISNWFAATIHNDAPDYRAFGGKLIQSLRYGENPHQHAAFYALPGRRPGV
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECIAEFDPARTAACVIVKHANPCGVAEGPDLISAYQK
CCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSTSAFGGIIAMNRKLDAATARAITGIFTEVIIAPDATEEAIAVVAARKNLRLLLA
HHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEEE
GALPDPREAGLTAKTVAGGLLVQSRDNAVVDDMTLKVVTKRAPTDAELRDLRFAFRVAKH
ECCCCHHHCCCCHHHHHCCEEEECCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHHH
VKSNTIVYAKDSATVGIGAGQMSRVDSARIAARKALDAAAELKLAEPLTKGSVVASDAFF
HCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADEHGIAMVLTGVRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]