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The map label for this gene is nodB [H]

Identifier: 146337448

GI number: 146337448

Start: 301471

End: 302511

Strand: Direct

Name: nodB [H]

Synonym: BRADO0288

Alternate gene names: 146337448

Gene position: 301471-302511 (Clockwise)

Preceding gene: 146337445

Following gene: 146337450

Centisome position: 4.04

GC content: 65.32

Gene sequence:

>1041_bases
ATGCGTAATGCGTTAGGCTTGCTGCTGGCCACCGTCGTTGCGGCGCTGGCGATCACCGGCGTCTGGTACTGGGTTTCAAA
GCCGAACGCCGCCAAGGCTGCCGCGCAGACCGCTGCGGCGCAGCCGTCCGAGCCTGCACCCGCACCGGCGAAACTGGCCG
CCAAGGGGGCCAAGGACGATGTCGACTCTACCGGCACCGTGCCGGCCAAGTCCGCATCGACTGCCGCGCCGCCCATGCCG
CCGAAGTCCAATTGCGCCAATCCGGATGCGCTCGGCATCTCCCGCACCGTCGTGGTCGACACCACCGGCGGCCCTGGTTT
CGGCCTGGACCATTTCAAGCAGTTCGACTTCCTTGCCGACCGCGAGGTCGTGCTGACCTTCGATGACGGCCCGTGGCCGG
GCAACACGCCGGCGGTGCTGAAGGCGCTGGCCGACGAGTGCACCACCGGCATATTCTTCTCCATCGGCAAGCACGCGACC
TATCATCCGGAGATCCTGCGCCAGGTGCTGGCGGCTGGTCACGTGGTCGGCGTGCACACCTGGTCGCACGCCAATCTCAA
CGGCAAGAAGATGACCGACGAGATGGCCAAGGAAGAGCTCGAGAAGGGCTTCAGCGCGGTCAAGTTCGCGCTCGGCACCA
ACCCCGCGCCATTCTTTCGCTTCCCGCAGCTGCAGCACCGGCCGTCGGCGGTGGCCTATCTCGGCAGCCGCAACGTCGCG
ATGTTCTCCTGCGACGTCGACAGCTTCGACTTCCGCTCCAAGGATGCCGACCAGGTCGTGAACACTGTCATGACCGGGCT
CGAGAAGAAGGGCAAGGGCATCATCCTGATGCACGATTTCCAGAAGAACACCGCGCTGGCGCTGCCGACCCTGCTGCGGC
GGCTGAAGGCCGGCGGCTACAAGGTCGTCGCGATGAAGCCGAAGGGCACGCTCGACACGATGCCCGAATATGACGCGATG
ATCGGCCAGGATCCGAAGCTGCCGACCACCATGACCAATGCGCGGCCGATCTCCAGCGTGATCCAGACCGTTCAGCAGTG
A

Upstream 100 bases:

>100_bases
ACCCCGTTAACGTTTCGTTGGCACTAATGGAAGCTGATTGAGGGGCCGGACACCGCCGTGTCCGTGCATCTCGTGTCTCG
TGTTTTCGGGAAGAGTAACC

Downstream 100 bases:

>100_bases
CGCCGCCAACCGTTTGGCCGCCGACGGCTTGCAAAACGCGAAGGCCGGGCAGCAGCCCGGCCTTTCTCGTCTCTCGGCTG
CGTCCAGCAGCTCACCAATA

Product: putative polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRNALGLLLATVVAALAITGVWYWVSKPNAAKAAAQTAAAQPSEPAPAPAKLAAKGAKDDVDSTGTVPAKSASTAAPPMP
PKSNCANPDALGISRTVVVDTTGGPGFGLDHFKQFDFLADREVVLTFDDGPWPGNTPAVLKALADECTTGIFFSIGKHAT
YHPEILRQVLAAGHVVGVHTWSHANLNGKKMTDEMAKEELEKGFSAVKFALGTNPAPFFRFPQLQHRPSAVAYLGSRNVA
MFSCDVDSFDFRSKDADQVVNTVMTGLEKKGKGIILMHDFQKNTALALPTLLRRLKAGGYKVVAMKPKGTLDTMPEYDAM
IGQDPKLPTTMTNARPISSVIQTVQQ

Sequences:

>Translated_346_residues
MRNALGLLLATVVAALAITGVWYWVSKPNAAKAAAQTAAAQPSEPAPAPAKLAAKGAKDDVDSTGTVPAKSASTAAPPMP
PKSNCANPDALGISRTVVVDTTGGPGFGLDHFKQFDFLADREVVLTFDDGPWPGNTPAVLKALADECTTGIFFSIGKHAT
YHPEILRQVLAAGHVVGVHTWSHANLNGKKMTDEMAKEELEKGFSAVKFALGTNPAPFFRFPQLQHRPSAVAYLGSRNVA
MFSCDVDSFDFRSKDADQVVNTVMTGLEKKGKGIILMHDFQKNTALALPTLLRRLKAGGYKVVAMKPKGTLDTMPEYDAM
IGQDPKLPTTMTNARPISSVIQTVQQ
>Mature_346_residues
MRNALGLLLATVVAALAITGVWYWVSKPNAAKAAAQTAAAQPSEPAPAPAKLAAKGAKDDVDSTGTVPAKSASTAAPPMP
PKSNCANPDALGISRTVVVDTTGGPGFGLDHFKQFDFLADREVVLTFDDGPWPGNTPAVLKALADECTTGIFFSIGKHAT
YHPEILRQVLAAGHVVGVHTWSHANLNGKKMTDEMAKEELEKGFSAVKFALGTNPAPFFRFPQLQHRPSAVAYLGSRNVA
MFSCDVDSFDFRSKDADQVVNTVMTGLEKKGKGIILMHDFQKNTALALPTLLRRLKAGGYKVVAMKPKGTLDTMPEYDAM
IGQDPKLPTTMTNARPISSVIQTVQQ

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323338, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 36857; Mature: 36857

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNALGLLLATVVAALAITGVWYWVSKPNAAKAAAQTAAAQPSEPAPAPAKLAAKGAKDD
CCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCC
VDSTGTVPAKSASTAAPPMPPKSNCANPDALGISRTVVVDTTGGPGFGLDHFKQFDFLAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC
REVVLTFDDGPWPGNTPAVLKALADECTTGIFFSIGKHATYHPEILRQVLAAGHVVGVHT
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEEEE
WSHANLNGKKMTDEMAKEELEKGFSAVKFALGTNPAPFFRFPQLQHRPSAVAYLGSRNVA
ECCCCCCCHHHHHHHHHHHHHCCHHHEEHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
MFSCDVDSFDFRSKDADQVVNTVMTGLEKKGKGIILMHDFQKNTALALPTLLRRLKAGGY
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCE
KVVAMKPKGTLDTMPEYDAMIGQDPKLPTTMTNARPISSVIQTVQQ
EEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRNALGLLLATVVAALAITGVWYWVSKPNAAKAAAQTAAAQPSEPAPAPAKLAAKGAKDD
CCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCC
VDSTGTVPAKSASTAAPPMPPKSNCANPDALGISRTVVVDTTGGPGFGLDHFKQFDFLAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC
REVVLTFDDGPWPGNTPAVLKALADECTTGIFFSIGKHATYHPEILRQVLAAGHVVGVHT
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEEEE
WSHANLNGKKMTDEMAKEELEKGFSAVKFALGTNPAPFFRFPQLQHRPSAVAYLGSRNVA
ECCCCCCCHHHHHHHHHHHHHCCHHHEEHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
MFSCDVDSFDFRSKDADQVVNTVMTGLEKKGKGIILMHDFQKNTALALPTLLRRLKAGGY
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCE
KVVAMKPKGTLDTMPEYDAMIGQDPKLPTTMTNARPISSVIQTVQQ
EEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968; 8850088 [H]