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The map label for this gene is folD [H]

Identifier: 146337437

GI number: 146337437

Start: 290848

End: 291732

Strand: Reverse

Name: folD [H]

Synonym: BRADO0277

Alternate gene names: 146337437

Gene position: 291732-290848 (Counterclockwise)

Preceding gene: 146337438

Following gene: 146337433

Centisome position: 3.91

GC content: 68.47

Gene sequence:

>885_bases
ATGACCGCACGCATCATCGACGGGAAGATCATCGCGGCCGACCTGCGCGCGCAGGTCGCGCGGGAGGTGGAGCGGGTCAA
GCACGACCTTGGCCTGACGCCGGGGCTTGCGGTGGTGCTGGTCGGCAACGACCCGGCCTCCGAGGTTTACGTCCGCAGCA
AGCACACCCAGACCCAGGCCGCTGGCATGGCTTCGTTCGAGCACAAGCTGCCGGCCGACGTCGCTCAGGCTGAGCTGCTG
GCGCTGATCTCGAAGCTCAATCAGGACCCCTCGGTGCATGGCATCCTGGTGCAGCTGCCGCTGCCGAAAGGGTTGGACAC
CGAGGCCGTGATCAATGCCATCGACCCGGCCAAGGATGTCGACGGCCTACATCCGCACAATGCCGGCCGGCTCGCCGGCG
GCCAGCCGGCGCTGGCGCCGTGCACGCCGCTCGGCTGCATCATCCTCAGCAAGACCGTGCATGCCTCGCTGGAGGGCATG
AATGCGATCGTCATCGGCCGCTCCAACCTGGTCGGCCGCCCCCTGGTGCAGCTGCTTCTGAACGAGAACGCCACTGTCAC
GATCGCCCATTCGCGCTCGCGCGATCTGCCGGCGCTGACTGCGCGCGCCGATCTCGTCTATGCGGCGGTCGGCCGTCCGG
AGATGGTGAAGCGCGACTGGATCAAGCCGGGCGCGACCGTGATCGACGTCGGCATCAACCGCATTCCGACGCCTGAGGGC
AAGACCAGGCTGGTCGGGGATGTCGCCTATGCCGAGGTCGCTGAAGTCGCCGGCGCGATCACGCCGGTGCCGGGCGGCGT
CGGCCAGATGACGGTGGCCTGCCTGCTGGTGAACACGCTGCGCGCGGCCTGCGCCATCGCCGGGTTGCCGAAGCCTTCGG
TCTAG

Upstream 100 bases:

>100_bases
TGCCAGCCGTGGTCCACACCCTCCCCTGGAGAGGCGGGAGCCTGCGGCAGCGATCTTGCTGTCGACGAACTGAATAGGCG
CTGCGACGAGGGGACCACGC

Downstream 100 bases:

>100_bases
GCCGGACCAGCGGCTGTCTCCAGCAGCCGCCGATAGAAGCGGATCATGTCGCCATAGCCTTCGACCGACAGCCGCTCATT
GGTGCCGTGAAAGCGCTTGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTARIIDGKIIAADLRAQVAREVERVKHDLGLTPGLAVVLVGNDPASEVYVRSKHTQTQAAGMASFEHKLPADVAQAELL
ALISKLNQDPSVHGILVQLPLPKGLDTEAVINAIDPAKDVDGLHPHNAGRLAGGQPALAPCTPLGCIILSKTVHASLEGM
NAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRDLPALTARADLVYAAVGRPEMVKRDWIKPGATVIDVGINRIPTPEG
KTRLVGDVAYAEVAEVAGAITPVPGGVGQMTVACLLVNTLRAACAIAGLPKPSV

Sequences:

>Translated_294_residues
MTARIIDGKIIAADLRAQVAREVERVKHDLGLTPGLAVVLVGNDPASEVYVRSKHTQTQAAGMASFEHKLPADVAQAELL
ALISKLNQDPSVHGILVQLPLPKGLDTEAVINAIDPAKDVDGLHPHNAGRLAGGQPALAPCTPLGCIILSKTVHASLEGM
NAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRDLPALTARADLVYAAVGRPEMVKRDWIKPGATVIDVGINRIPTPEG
KTRLVGDVAYAEVAEVAGAITPVPGGVGQMTVACLLVNTLRAACAIAGLPKPSV
>Mature_293_residues
TARIIDGKIIAADLRAQVAREVERVKHDLGLTPGLAVVLVGNDPASEVYVRSKHTQTQAAGMASFEHKLPADVAQAELLA
LISKLNQDPSVHGILVQLPLPKGLDTEAVINAIDPAKDVDGLHPHNAGRLAGGQPALAPCTPLGCIILSKTVHASLEGMN
AIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRDLPALTARADLVYAAVGRPEMVKRDWIKPGATVIDVGINRIPTPEGK
TRLVGDVAYAEVAEVAGAITPVPGGVGQMTVACLLVNTLRAACAIAGLPKPSV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=45.9731543624161, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=44.7457627118644, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI94721354, Length=303, Percent_Identity=42.5742574257426, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI36796743, Length=201, Percent_Identity=28.8557213930348, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=49.2957746478873, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=41.9243986254296, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=47.3684210526316, Blast_Score=261, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=43.0976430976431, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=27.0096463022508, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=45.6445993031359, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=45.6445993031359, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=45.6445993031359, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=45.6445993031359, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=46.5753424657534, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=46.5753424657534, Blast_Score=230, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30626; Mature: 30495

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARIIDGKIIAADLRAQVAREVERVKHDLGLTPGLAVVLVGNDPASEVYVRSKHTQTQA
CCCEEECCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCHHHH
AGMASFEHKLPADVAQAELLALISKLNQDPSVHGILVQLPLPKGLDTEAVINAIDPAKDV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHC
DGLHPHNAGRLAGGQPALAPCTPLGCIILSKTVHASLEGMNAIVIGRSNLVGRPLVQLLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRDLPALTARADLVYAAVGRPEMVKRDWIKPGATVIDVGINRIPTPEG
CCCCEEEEEECCCCCCCCHHHHHHEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCC
KTRLVGDVAYAEVAEVAGAITPVPGGVGQMTVACLLVNTLRAACAIAGLPKPSV
CCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TARIIDGKIIAADLRAQVAREVERVKHDLGLTPGLAVVLVGNDPASEVYVRSKHTQTQA
CCEEECCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCHHHH
AGMASFEHKLPADVAQAELLALISKLNQDPSVHGILVQLPLPKGLDTEAVINAIDPAKDV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHC
DGLHPHNAGRLAGGQPALAPCTPLGCIILSKTVHASLEGMNAIVIGRSNLVGRPLVQLLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRDLPALTARADLVYAAVGRPEMVKRDWIKPGATVIDVGINRIPTPEG
CCCCEEEEEECCCCCCCCHHHHHHEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCC
KTRLVGDVAYAEVAEVAGAITPVPGGVGQMTVACLLVNTLRAACAIAGLPKPSV
CCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA