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The map label for this gene is rlmN [H]

Identifier: 146337410

GI number: 146337410

Start: 263654

End: 264865

Strand: Reverse

Name: rlmN [H]

Synonym: BRADO0246

Alternate gene names: 146337410

Gene position: 264865-263654 (Counterclockwise)

Preceding gene: 146337411

Following gene: 146337409

Centisome position: 3.55

GC content: 65.51

Gene sequence:

>1212_bases
ATGGCAACGACTGCCGGGCTGCGCGACGCCGCCGCGCCCCTCGAAAAGATTCCGCTCGAAACCTACGTGCCGCCGGCCAA
GCCGTCGCTGATCGGTCTGTCGCGCGCGGAGCTGGCGGCCAGGCTCGGCGACATCGGCGTGCCGGAGCGGCAGCAGAAGA
TGCGGGTGCAGCAGCTGTGGCATTGGCTCTACTTCCGCGGCGCCCGATCGTTCGACGAGATGACCTCGGTGTCGAAGGAC
ACGCGCAACGGCCTGGCCGAGCGCTTCACCGTCGACCGTCCCGAGGTCGTGGCCGAGCAGATCTCCAATGACGGCACCCG
CAAATGGCTGCTACGGCTGCCGAGCGGCGACGACCTGCAGAAGGCGCACGAGGTCGAATGCGTCTACATTCCGGAGACCG
ACCGCGGCACCCTGTGCGTCTCTTCCCAGGTCGGCTGCACGCTGAACTGCGCGTTCTGCCACACCGGTACGCAGCGTCTG
GTGCGCAATCTCACCGCGGGCGAGATCGTCGGCCAGATCATGGTCGCCCGCGATCGCCTCAATGACTGGGCCGATCGCGA
AACGCCGCACGGCAATCGCCTCATCACCAACATCGTGATGATGGGCATGGGCGAGCCGCTGTACAATTTCGAAGCGGTGC
GCGACGCGCTGCTGATCGTCTCCGACAACGAGGGCATCGGCATCTCCCGCCGCCGCATCACGCTGTCGACGTCGGGCGTC
GTGCCGAACATCAAGCGCGCCGGCGAGGAGATCGGCGTGATGCTGGCGATCTCGCTGCATGCGGTCCGCGACGAGCTGCG
CAACGAGCTGGTGCCGCTCAACCGGAAGTATCCGATCGCCGAGCTGCTGCAGGCCTGCCGCGACTATCCGGGCGCGTCAA
ATGCCCGCCGCATCACCTTCGAATATGTGATGCTCAAGGGCGTCAACGATTCGCTCGACGACGCCAGACTGCTGGTCAAG
CTGCTCAAGGGCATTCCGGCCAAGATCAATTTGATCCCGTTCAATCCTTGGCCGGGCTCGGCCTACGAATGCTCGGACTG
GGAGCAGATCGAGAAGTTCTCCGAATATGTCTTCAATGCCGGCTACTCCTCCCCGGTGCGCACCCCCCGCGGCCGCGACA
TCCTCGCCGCCTGCGGCCAGCTGAAGTCGGAAACGGAGAAGCTCTCCGCCAGAGAACGCCAGGCGCTGCGCGCGATGGCG
ATGACGGATTGA

Upstream 100 bases:

>100_bases
GCCGCTGGTCTTGCGGGTCGCTTTTGGCGGCCGAATTGCTATATAGACCCGTTGAAAGCCTCAGGCCGCAGCGGCCGGGC
AGCATTTTGGGCCCCTGGAC

Downstream 100 bases:

>100_bases
TTCTTGATCGTCAGCAATTCCAAGCAACGTCGTCATGCCAGGGCTCATCCATTCCTATCCGCAAAAAGTCTTCAGTGATT
GATGGCCGGGTCGAGCCCGG

Product: putative pyruvate formate lyase activating enzyme 2 (yfgB)

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MATTAGLRDAAAPLEKIPLETYVPPAKPSLIGLSRAELAARLGDIGVPERQQKMRVQQLWHWLYFRGARSFDEMTSVSKD
TRNGLAERFTVDRPEVVAEQISNDGTRKWLLRLPSGDDLQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRL
VRNLTAGEIVGQIMVARDRLNDWADRETPHGNRLITNIVMMGMGEPLYNFEAVRDALLIVSDNEGIGISRRRITLSTSGV
VPNIKRAGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARLLVK
LLKGIPAKINLIPFNPWPGSAYECSDWEQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMA
MTD

Sequences:

>Translated_403_residues
MATTAGLRDAAAPLEKIPLETYVPPAKPSLIGLSRAELAARLGDIGVPERQQKMRVQQLWHWLYFRGARSFDEMTSVSKD
TRNGLAERFTVDRPEVVAEQISNDGTRKWLLRLPSGDDLQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRL
VRNLTAGEIVGQIMVARDRLNDWADRETPHGNRLITNIVMMGMGEPLYNFEAVRDALLIVSDNEGIGISRRRITLSTSGV
VPNIKRAGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARLLVK
LLKGIPAKINLIPFNPWPGSAYECSDWEQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMA
MTD
>Mature_402_residues
ATTAGLRDAAAPLEKIPLETYVPPAKPSLIGLSRAELAARLGDIGVPERQQKMRVQQLWHWLYFRGARSFDEMTSVSKDT
RNGLAERFTVDRPEVVAEQISNDGTRKWLLRLPSGDDLQKAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLV
RNLTAGEIVGQIMVARDRLNDWADRETPHGNRLITNIVMMGMGEPLYNFEAVRDALLIVSDNEGIGISRRRITLSTSGVV
PNIKRAGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAELLQACRDYPGASNARRITFEYVMLKGVNDSLDDARLLVKL
LKGIPAKINLIPFNPWPGSAYECSDWEQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMAM
TD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=370, Percent_Identity=46.7567567567568, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 44983; Mature: 44852

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTAGLRDAAAPLEKIPLETYVPPAKPSLIGLSRAELAARLGDIGVPERQQKMRVQQLW
CCCCCCCCHHHCHHHHCCHHHCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHH
HWLYFRGARSFDEMTSVSKDTRNGLAERFTVDRPEVVAEQISNDGTRKWLLRLPSGDDLQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCH
KAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAGEIVGQIMVARDRL
HHHCCEEEEECCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHCHHHHHHHHHHHHHHHH
NDWADRETPHGNRLITNIVMMGMGEPLYNFEAVRDALLIVSDNEGIGISRRRITLSTSGV
HHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCC
VPNIKRAGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAELLQACRDYPGASNARRITF
CCCHHHHHHHHCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
EYVMLKGVNDSLDDARLLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIEKFSEYVFNA
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMAMTD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATTAGLRDAAAPLEKIPLETYVPPAKPSLIGLSRAELAARLGDIGVPERQQKMRVQQLW
CCCCCCCHHHCHHHHCCHHHCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHH
HWLYFRGARSFDEMTSVSKDTRNGLAERFTVDRPEVVAEQISNDGTRKWLLRLPSGDDLQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCH
KAHEVECVYIPETDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAGEIVGQIMVARDRL
HHHCCEEEEECCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHCHHHHHHHHHHHHHHHH
NDWADRETPHGNRLITNIVMMGMGEPLYNFEAVRDALLIVSDNEGIGISRRRITLSTSGV
HHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCCEEEEEEEECCCC
VPNIKRAGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAELLQACRDYPGASNARRITF
CCCHHHHHHHHCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
EYVMLKGVNDSLDDARLLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIEKFSEYVFNA
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRAMAMTD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA