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The map label for this gene is xthA [H]

Identifier: 146337348

GI number: 146337348

Start: 193090

End: 193905

Strand: Direct

Name: xthA [H]

Synonym: BRADO0181

Alternate gene names: 146337348

Gene position: 193090-193905 (Clockwise)

Preceding gene: 146337347

Following gene: 146337351

Centisome position: 2.59

GC content: 63.11

Gene sequence:

>816_bases
ATGCGTCTCGTCCTGACCACCTGGAACATCAATTCGGTGCGCCTGCGCATCGATCTCGTCGCCAAGTTCATCAAGCAGGT
CCGGCCCGATGTGCTGTGCCTGCAGGAGACCAAGTGCATCGACGATGCCTTTCCGCTGAAGCGCTTCAAGCGGCTGGGTT
ATGAGCATGTCGCGCTGAACGGACAGAAGGGCTATCACGGCGTCGCGGTGATCTCGAAATGGCCGTTCGAGTCCACCCAT
GTCCGCACCTTCTGCGACAAGGTCGATTCGCGTCACATCACCGTAGCCTTCGGCGAGAAGGCGCAGCTGGCGCAGCCGCT
GGTGCTGCATAATTTCTATGTGCCGGCGGGCGGCGACATTCCCGATCCCGCGCTCAATCCGAAGTTCGAGCACAAGCTGC
AATTCCTGGACGAGATGAAGGCGTGCGAGCCGCTGCATCCGCGCGGCGACGACCGCCACATCCTGGTCGGCGATCTCAAC
GTCGCGCCGCACGAGCACGACGTCTGGTCGCACAAGCAGCTGCTGAAGGTCGTCTCGCACACGCCGATCGAAACCGAGAA
GCTCTTGGCGGCGCAGAGCCATGGCGAATGGGTCGACGTCGCGCGCGAGCGCATCCCGATGTCGGAGAAGGTCTATACAT
GGTGGAGCTATCGCGCCGCCGACTGGACCGTCGGCGACCGTGGCCGAAGGCTGGACCACATCTGGGTGTCACGCGCGCTG
AAGGACCGCGTCAGCGACTTCGACATCCTACGTGACGCGCGCAGCTGGGAGCGGCCGTCGGATCACGTGCCGGTCACCGT
GACCTTGGACGTTTGA

Upstream 100 bases:

>100_bases
ATGAACCAACTCTCACACCGAGGCTGCACATCGTTGCTGGGGATAAGGCCGGCGGAGACGCCGCTGACGCCGCCGCATTC
CATGCTATGACGCGATCCTC

Downstream 100 bases:

>100_bases
CGGCGTGAGCCGGCGTTATTTGTTCACCCGCCTCCACGCCGTCATGGCCGGGCATCGTCCAGGACAAGACCGGGCATGAC
GACCCGGGTACTAGAATCGA

Product: putative exodeoxyribonuclease III (xthA)

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRLVLTTWNINSVRLRIDLVAKFIKQVRPDVLCLQETKCIDDAFPLKRFKRLGYEHVALNGQKGYHGVAVISKWPFESTH
VRTFCDKVDSRHITVAFGEKAQLAQPLVLHNFYVPAGGDIPDPALNPKFEHKLQFLDEMKACEPLHPRGDDRHILVGDLN
VAPHEHDVWSHKQLLKVVSHTPIETEKLLAAQSHGEWVDVARERIPMSEKVYTWWSYRAADWTVGDRGRRLDHIWVSRAL
KDRVSDFDILRDARSWERPSDHVPVTVTLDV

Sequences:

>Translated_271_residues
MRLVLTTWNINSVRLRIDLVAKFIKQVRPDVLCLQETKCIDDAFPLKRFKRLGYEHVALNGQKGYHGVAVISKWPFESTH
VRTFCDKVDSRHITVAFGEKAQLAQPLVLHNFYVPAGGDIPDPALNPKFEHKLQFLDEMKACEPLHPRGDDRHILVGDLN
VAPHEHDVWSHKQLLKVVSHTPIETEKLLAAQSHGEWVDVARERIPMSEKVYTWWSYRAADWTVGDRGRRLDHIWVSRAL
KDRVSDFDILRDARSWERPSDHVPVTVTLDV
>Mature_271_residues
MRLVLTTWNINSVRLRIDLVAKFIKQVRPDVLCLQETKCIDDAFPLKRFKRLGYEHVALNGQKGYHGVAVISKWPFESTH
VRTFCDKVDSRHITVAFGEKAQLAQPLVLHNFYVPAGGDIPDPALNPKFEHKLQFLDEMKACEPLHPRGDDRHILVGDLN
VAPHEHDVWSHKQLLKVVSHTPIETEKLLAAQSHGEWVDVARERIPMSEKVYTWWSYRAADWTVGDRGRRLDHIWVSRAL
KDRVSDFDILRDARSWERPSDHVPVTVTLDV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=31.021897810219, Blast_Score=110, Evalue=1e-25,
Organism=Drosophila melanogaster, GI221330655, Length=276, Percent_Identity=28.9855072463768, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136678, Length=276, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31310; Mature: 31310

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVLTTWNINSVRLRIDLVAKFIKQVRPDVLCLQETKCIDDAFPLKRFKRLGYEHVALN
CEEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHCCCEEEEEC
GQKGYHGVAVISKWPFESTHVRTFCDKVDSRHITVAFGEKAQLAQPLVLHNFYVPAGGDI
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCEEHHEEECCCCCCC
PDPALNPKFEHKLQFLDEMKACEPLHPRGDDRHILVGDLNVAPHEHDVWSHKQLLKVVSH
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
TPIETEKLLAAQSHGEWVDVARERIPMSEKVYTWWSYRAADWTVGDRGRRLDHIWVSRAL
CCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
KDRVSDFDILRDARSWERPSDHVPVTVTLDV
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRLVLTTWNINSVRLRIDLVAKFIKQVRPDVLCLQETKCIDDAFPLKRFKRLGYEHVALN
CEEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHCCCEEEEEC
GQKGYHGVAVISKWPFESTHVRTFCDKVDSRHITVAFGEKAQLAQPLVLHNFYVPAGGDI
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCEEHHEEECCCCCCC
PDPALNPKFEHKLQFLDEMKACEPLHPRGDDRHILVGDLNVAPHEHDVWSHKQLLKVVSH
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
TPIETEKLLAAQSHGEWVDVARERIPMSEKVYTWWSYRAADWTVGDRGRRLDHIWVSRAL
CCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
KDRVSDFDILRDARSWERPSDHVPVTVTLDV
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]