Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 146337321

GI number: 146337321

Start: 166604

End: 167437

Strand: Reverse

Name: nth [H]

Synonym: BRADO0152

Alternate gene names: 146337321

Gene position: 167437-166604 (Counterclockwise)

Preceding gene: 146337324

Following gene: 146337320

Centisome position: 2.25

GC content: 66.91

Gene sequence:

>834_bases
ATGGCCAAAATCACCCGCCCCCAACGCGCCAAGCCGGCCCCGGTTCGCACGCCCGCCAGAAAGGCGGCCAAGGCGGCGCC
GGGAGCAGCGTCCAAATCCGCATCCAAAACGGTCAAGAAGGCTGCCAAGATCAGCCCGGCGGCCAAGCTGAAGGCAGGCT
CGGTCAAGCCGGTCAAGAAAGCGTTCAAGGCGACGAAACCCTGGACACCCATGGAGATCCGCGAGGCCTTCAGCCGGTTC
GCTCAGGCCAATCCGGAGCCGAAGGGCGAGCTCGAGCATCTCAACCCGTACACCTTGCTGGTGGCCGTGGTGCTGTCGGC
GCAGGCGACCGACGCCGGCGTCAACAAGGCGACGCGGCCGCTGTTCGCGGTCGCCGATACGCCGCAGAAGATGCTCGCGC
TCGGCGAGGACACGGTGCGCGACTACATCAAGACCGTCGGCTTGTTCCGCACCAAAGCCAAGAACGTGATCGCGCTGTCG
CAAAAGCTGATCGCCGAGTTCGGCGGCGAGGTGCCGCGCACGCGCGCCGAATTGGAGTCGCTGCCGGGCGCGGGGCGCAA
GACCGCGAATGTCGTGCTCAACATGGCGTTCGGCGAGCACACGATGGCGGTCGACACCCATGTGTTCCGCGTCGGCAACC
GCACGGGCTTAGCTCCGGGCAAGACGCCGCTGGAGGTCGAGCTCGGGCTGGAGAAGGTGATCCCGGCGGAGTTCATGCTG
CACGCCCATCACTGGCTGATCCTGCACGGCCGCTACACCTGCCTCGCCCGCAAGCCGCGCTGCGAGCTGTGCCTGATCAA
GGACCTCTGCCGCTGGCCGGAGAAGACGGCGTGA

Upstream 100 bases:

>100_bases
CAGCCGGGCCGAGAACAGCTCGGGATCAGCGGCGTCAGTGCCGGTCTCCTGATACGCAAAAGCATTGCCTGGCGTCATGG
GGCCTCAGTATACCCGGATC

Downstream 100 bases:

>100_bases
TCAGGAGCGCGGCTGCCCCAGGCAGCGGTCGTTTCCGGCTCGACCTCTCGCTGTCCAGCTCCGCTGTCATGCCCCGCGAA
GGCGGGGCATCCAGTACGCC

Product: endonuclease III DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MAKITRPQRAKPAPVRTPARKAAKAAPGAASKSASKTVKKAAKISPAAKLKAGSVKPVKKAFKATKPWTPMEIREAFSRF
AQANPEPKGELEHLNPYTLLVAVVLSAQATDAGVNKATRPLFAVADTPQKMLALGEDTVRDYIKTVGLFRTKAKNVIALS
QKLIAEFGGEVPRTRAELESLPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGKTPLEVELGLEKVIPAEFML
HAHHWLILHGRYTCLARKPRCELCLIKDLCRWPEKTA

Sequences:

>Translated_277_residues
MAKITRPQRAKPAPVRTPARKAAKAAPGAASKSASKTVKKAAKISPAAKLKAGSVKPVKKAFKATKPWTPMEIREAFSRF
AQANPEPKGELEHLNPYTLLVAVVLSAQATDAGVNKATRPLFAVADTPQKMLALGEDTVRDYIKTVGLFRTKAKNVIALS
QKLIAEFGGEVPRTRAELESLPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGKTPLEVELGLEKVIPAEFML
HAHHWLILHGRYTCLARKPRCELCLIKDLCRWPEKTA
>Mature_276_residues
AKITRPQRAKPAPVRTPARKAAKAAPGAASKSASKTVKKAAKISPAAKLKAGSVKPVKKAFKATKPWTPMEIREAFSRFA
QANPEPKGELEHLNPYTLLVAVVLSAQATDAGVNKATRPLFAVADTPQKMLALGEDTVRDYIKTVGLFRTKAKNVIALSQ
KLIAEFGGEVPRTRAELESLPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGKTPLEVELGLEKVIPAEFMLH
AHHWLILHGRYTCLARKPRCELCLIKDLCRWPEKTA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=35.0282485875706, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=59.2039800995025, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1789331, Length=180, Percent_Identity=28.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=31.8681318681319, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=185, Percent_Identity=26.4864864864865, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=30.5084745762712, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 30091; Mature: 29959

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITRPQRAKPAPVRTPARKAAKAAPGAASKSASKTVKKAAKISPAAKLKAGSVKPVKK
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
AFKATKPWTPMEIREAFSRFAQANPEPKGELEHLNPYTLLVAVVLSAQATDAGVNKATRP
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCC
LFAVADTPQKMLALGEDTVRDYIKTVGLFRTKAKNVIALSQKLIAEFGGEVPRTRAELES
EEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGKTPLEVELGLEKVIPAEFML
CCCCCCCCCCEEEEEECCCCEEEHHHHEEEECCCCCCCCCCCCEEEECCHHHHCHHHHHH
HAHHWLILHGRYTCLARKPRCELCLIKDLCRWPEKTA
HHCEEEEEECCEEEEECCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKITRPQRAKPAPVRTPARKAAKAAPGAASKSASKTVKKAAKISPAAKLKAGSVKPVKK
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
AFKATKPWTPMEIREAFSRFAQANPEPKGELEHLNPYTLLVAVVLSAQATDAGVNKATRP
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCC
LFAVADTPQKMLALGEDTVRDYIKTVGLFRTKAKNVIALSQKLIAEFGGEVPRTRAELES
EEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGKTPLEVELGLEKVIPAEFML
CCCCCCCCCCEEEEEECCCCEEEHHHHEEEECCCCCCCCCCCCEEEECCHHHHCHHHHHH
HAHHWLILHGRYTCLARKPRCELCLIKDLCRWPEKTA
HHCEEEEEECCEEEEECCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]