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The map label for this gene is yafC [H]

Identifier: 146337191

GI number: 146337191

Start: 22286

End: 23206

Strand: Reverse

Name: yafC [H]

Synonym: BRADO0017

Alternate gene names: 146337191

Gene position: 23206-22286 (Counterclockwise)

Preceding gene: 146337194

Following gene: 146337188

Centisome position: 0.31

GC content: 68.84

Gene sequence:

>921_bases
ATGTCGAGCACCCGCTTACCCGATTTCGAGGGCCTCGCCCTGCTCGCGAGGGTGGCAGAGGAACGCTCGTTCGCCGGCGC
TGCCCGCGCCTTGAACCTTTCGGTGGCAACGGTCTCCCGGGCCATCACCCGCCTGGAGGACCGGCTCGGCGCGCGGGTGC
TCAATCGGACCTCGCGCAAGGTGGCGCTGACCGATTTCGGCCGGACGCTCGCCGAGCGCGGCGCGCGCATGCTGCGGGAG
GCGGAGGAGGCGGAAAGCGCCGCGCGCGAACTGTCCAGCCGTCCGCGCGGCACCGTCAATCTCGCCGTGCCGATGTCGTT
CGGCCTGCGCGAAGTCAGCCCGCTGCTTCCCGCGTTCGTGCGCGAATACCCGGAGATCACGCTCAACCTGCATCTCAGCG
ACGCCACCGTCGATCTGATCGGCGACGGCTTCGATGCCGCGTTGCGGATCGCCGCACTGCCGGACTCGTCGTTGATCGCC
CGGCGCCTGCGCCCAATGGGCCGCTTCCTGGTGGCGTCGCCGGACTACATCGCCCGCTACGGCCGGCCGCAAACCCCCGC
CGAGCTGAACGTCCATCATTGCCTCGGCTATGCCTATCGCGCCCGGACGGCGACATGGCATCTGACCCACACCGACGGCC
GCGAGGCCGCGGTGACGCCGACCGGACCGCTGTCGGTGACGAACATCGATGCGCTGCTGCCCGCATTGCTGGACGGCATC
GGCATCGCCGAACTGCCATGCTTCGTCGCTGATCCCCACGTCGCCGAAGGGCGGCTGGAAATTCTGCTGCCGGACTGGAA
GCTCCCGGGCGGCGGGTTGTACTTCATCACGCCAGCCGCGCATGTGCGGCCGGCCCGGATCGAGGTGCTGAGTGAGTTTC
TCGCCAGACATTTGTCGCGTTCGCGCGAAGAACGCTGTTAG

Upstream 100 bases:

>100_bases
GCCATGGGGGTCTCTCCTGTCGTGTTGGCCAGCAGAGGATGGCGAAACGCCTGCAACTCCGATATAGAAACAGTTGAAAG
TCATCGTTGCAGGAAAGTGG

Downstream 100 bases:

>100_bases
CGGAACAAACAATTGCGGTTCGCAAAGGCGAGATTTGGGCTTGCTATTTTTCCGAAATCATGATCTATTCCGTGCATCCC
GACCTCATGCAGAGGGGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSTRLPDFEGLALLARVAEERSFAGAARALNLSVATVSRAITRLEDRLGARVLNRTSRKVALTDFGRTLAERGARMLRE
AEEAESAARELSSRPRGTVNLAVPMSFGLREVSPLLPAFVREYPEITLNLHLSDATVDLIGDGFDAALRIAALPDSSLIA
RRLRPMGRFLVASPDYIARYGRPQTPAELNVHHCLGYAYRARTATWHLTHTDGREAAVTPTGPLSVTNIDALLPALLDGI
GIAELPCFVADPHVAEGRLEILLPDWKLPGGGLYFITPAAHVRPARIEVLSEFLARHLSRSREERC

Sequences:

>Translated_306_residues
MSSTRLPDFEGLALLARVAEERSFAGAARALNLSVATVSRAITRLEDRLGARVLNRTSRKVALTDFGRTLAERGARMLRE
AEEAESAARELSSRPRGTVNLAVPMSFGLREVSPLLPAFVREYPEITLNLHLSDATVDLIGDGFDAALRIAALPDSSLIA
RRLRPMGRFLVASPDYIARYGRPQTPAELNVHHCLGYAYRARTATWHLTHTDGREAAVTPTGPLSVTNIDALLPALLDGI
GIAELPCFVADPHVAEGRLEILLPDWKLPGGGLYFITPAAHVRPARIEVLSEFLARHLSRSREERC
>Mature_305_residues
SSTRLPDFEGLALLARVAEERSFAGAARALNLSVATVSRAITRLEDRLGARVLNRTSRKVALTDFGRTLAERGARMLREA
EEAESAARELSSRPRGTVNLAVPMSFGLREVSPLLPAFVREYPEITLNLHLSDATVDLIGDGFDAALRIAALPDSSLIAR
RLRPMGRFLVASPDYIARYGRPQTPAELNVHHCLGYAYRARTATWHLTHTDGREAAVTPTGPLSVTNIDALLPALLDGIG
IAELPCFVADPHVAEGRLEILLPDWKLPGGGLYFITPAAHVRPARIEVLSEFLARHLSRSREERC

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=36.1774744027304, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=30.6620209059233, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787128, Length=272, Percent_Identity=31.6176470588235, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=29.7945205479452, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=31.5068493150685, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787589, Length=263, Percent_Identity=32.6996197718631, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=31.1036789297659, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=25.503355704698, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=269, Percent_Identity=28.2527881040892, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=32.8467153284672, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=172, Percent_Identity=27.906976744186, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=24.8251748251748, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790399, Length=124, Percent_Identity=29.8387096774194, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33468; Mature: 33337

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTRLPDFEGLALLARVAEERSFAGAARALNLSVATVSRAITRLEDRLGARVLNRTSRK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VALTDFGRTLAERGARMLREAEEAESAARELSSRPRGTVNLAVPMSFGLREVSPLLPAFV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
REYPEITLNLHLSDATVDLIGDGFDAALRIAALPDSSLIARRLRPMGRFLVASPDYIARY
HCCCCEEEEEEECCCEEEEECCCHHHEEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHC
GRPQTPAELNVHHCLGYAYRARTATWHLTHTDGREAAVTPTGPLSVTNIDALLPALLDGI
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCC
GIAELPCFVADPHVAEGRLEILLPDWKLPGGGLYFITPAAHVRPARIEVLSEFLARHLSR
CHHHCCCEECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
SREERC
HHHCCC
>Mature Secondary Structure 
SSTRLPDFEGLALLARVAEERSFAGAARALNLSVATVSRAITRLEDRLGARVLNRTSRK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VALTDFGRTLAERGARMLREAEEAESAARELSSRPRGTVNLAVPMSFGLREVSPLLPAFV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
REYPEITLNLHLSDATVDLIGDGFDAALRIAALPDSSLIARRLRPMGRFLVASPDYIARY
HCCCCEEEEEEECCCEEEEECCCHHHEEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHC
GRPQTPAELNVHHCLGYAYRARTATWHLTHTDGREAAVTPTGPLSVTNIDALLPALLDGI
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCC
GIAELPCFVADPHVAEGRLEILLPDWKLPGGGLYFITPAAHVRPARIEVLSEFLARHLSR
CHHHCCCEECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
SREERC
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]