| Definition | Flavobacterium johnsoniae UW101 chromosome, complete genome. |
|---|---|
| Accession | NC_009441 |
| Length | 6,096,872 |
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The map label for this gene is pcrA [H]
Identifier: 146301512
GI number: 146301512
Start: 4470831
End: 4473194
Strand: Direct
Name: pcrA [H]
Synonym: Fjoh_3773
Alternate gene names: 146301512
Gene position: 4470831-4473194 (Clockwise)
Preceding gene: 146301511
Following gene: 146301515
Centisome position: 73.33
GC content: 43.36
Gene sequence:
>2364_bases ATGAACGACCCGATTCTAGAAAATCTCAATCCCAGCCAGCTCCAAGCAGTGAAAACCACAGAAGGATACGTGCGTGTTAT TGCCGGTGCGGGATCTGGAAAAACAAAAGCCCTTACCTCCCGCTTTGCCTACATTGTGGACAGACTTGGCATAAACTCCT CCAATATACTCTGTGTAACCTTTACCAACAAGGCCGCCCAGGAAATGAAAAAACGCGTCAAGGCACTCATCGGTGATACC TATGATCTGGCTTTTATTACCACTTATCATGGCTTCTGCGTAAGGTTCCTCCGTGAAGAAATAAACAAAATACATTTTCC AAAGAATTTTATAATATTGGACGCGGAAGATCAGAAAAGCATATTGCGTGATATTTTCAACGAACTTCAGATCAACTCCA AACACCTGACTTTCAAACAGGTGCTGCGCTTTATATCCAAGCAGAAAAGCACTTCAAATTACCTGGGGTATATACTTGAA AATAAAGGGTTTGAACCTGACCCAAGTGATTCATTATCCAGCCGTATCTTTCAGATTTACCTGGACAAACTCAAACGCAA TTATGCTTTGGATTTTGATGATCTGATCCATTTTACTGCTTTTATTCTGGACCATAATCCCGATGTGCTTTTAAAATGGC AGCAAAACCTGCATTACATTCAGGTGGATGAGGCGCAGGACAGCTCAGAGAACCAGTTTCATCTGGTGGAAATCCTGTCT CGTGTACATCAAAACCTGTTCCTTGTCGGGGATCCCGACCAGACCATTTATGAATGGCGCGGGGCGAAACCGGAATATCT TGTTGAGTTTGACAGCATGTTCCCCGATACCCAGACCATTATCATGAACCGCAATTACCGCTCTACGCCGAACATCCTCA AGATCGGGAATCACATCATCAAGAACAATACGGTAAGGGTCGACAAAGACATGATCACCGACAAACCCGAAGGCTTTGAA GTGGTGCATTTTCACGGAAAGAATGACTTTGAGGAAAGCCAGTGGATTGCTTCTGAGATCAAGGAAATACTGCAGGCTGA ACAGGCCGCATATTCCGATATCACCATATTATACCGTTCGAATCACCTTTCAAGAAATATTGAACAGGCCCTTATCAAAG ATAATATTCCCTACTCTATTTTTGGGGGCATCCGTTTCTTTGAAAGAAAAGAGATTAAAGATGTGCTCTCTCTTTTAAGG CTCATTGTTCAGGGAGACAACTTCTCCTTTCTGCGCATGCACAACCACCCTGCCAGGGGACTTGGCAAAAAGTTCCTCGA AAGACTCAGTCTTCTGGCAGGGGAACAAAACCTTTCCCTTTTACAGGCATTGGAGCGCAACATCACCGACAAAGAACTGG CAAAAAAAGGCGCTGTTGAATTCCTGGAACTGATTGCAGAGCTGCGTGAAACAGCCAAAACAAAGTCCATCTCAGATCTG GTCAAAATTATACTCGATAAAAGCGGGCTTTCTGAACTATACCGCAAAGACGGTGATGAAGACAGGCTTGAGAACATTAA GGAACTTGTCAATTCGATGCTGCTGCTGGAAAAAGAAAACAATGCTCCGGTGGACATTATTGAGTATCTCCAGGAAATAT CCCTCTACACCGATCTGAATACTGATACCGAAAATCAGGACAAGGTTAAACTGATGACCATTCATATTTCGAAAGGACTT GAGTTTCCCTATGTCTTCCTGTGCGGTTTTACCGAAGGTGTACTGCCCAGCGCCCTTTCTGTCAAAGAAAGAAGGGCACG TGCTATTGAAGAGGAAAGAAGGCTCATGTATGTGGCCGTTACACGGGCAGAAAAACGATTTTACATGACTGATTCCGAAG GTTTTAATTTTACCACAGGACTCAATAAATATCCATCAAGGTTCCTTTTTGAGATCAAAGACGAATTTTATGTCCGCAAA GGAAAACTCTCCCAGCAGATTATCGAAAACAGCCGCAATCATCAAAGCCCTGGACAGCTGCAGGAAAATACAGCTTTCAA AGAAGGGGATTTCGTAATGCATCCGGTCTGGAATAAAGGAAAAATACAAACGGTCGACCTGGAAAAAAAAGAATATCTGG TGGATTTTTTTGATATGGGAAAAGTAAAACCCATCGATTTTGGGTTCAAACATCTGACGGGTTTCTCCGAAGACAGCGGG GATGCTGAAGGGCCGAAAGAACTTTTGGATCTGGCAGCGCATTTGAAAACAGCACCTGACCCTTTTGAGCAGGATACTGC TTTGAAACCACAGGGCATCACGTCGTTATCTTCTGACAGTATTTTTGATTCCGGCCCTATGCCGGATCTAAGTGAAAATA CGAAGCCAGACAAAAAAGATTCAAAAAAATGGTGGAAGCTTTAA
Upstream 100 bases:
>100_bases GATTTATTACCTCTTCAAATTAATTGTCTAAATTTGCATAACAGGTACTGATCTAACTGTTTTGTAAAAGGAATTAGATC AAAAAATATTGCTTCAAAAA
Downstream 100 bases:
>100_bases AAGTCCTGCTGCTGCTTCTCTATTTTTACATTTCATCCAGCAGCAATGCAAAAGCCCAGTGTTTTTTTATGTAAACAATG GGCTTTTGTACGTTTTCCGG
Product: UvrD/REP helicase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 787; Mature: 787
Protein sequence:
>787_residues MNDPILENLNPSQLQAVKTTEGYVRVIAGAGSGKTKALTSRFAYIVDRLGINSSNILCVTFTNKAAQEMKKRVKALIGDT YDLAFITTYHGFCVRFLREEINKIHFPKNFIILDAEDQKSILRDIFNELQINSKHLTFKQVLRFISKQKSTSNYLGYILE NKGFEPDPSDSLSSRIFQIYLDKLKRNYALDFDDLIHFTAFILDHNPDVLLKWQQNLHYIQVDEAQDSSENQFHLVEILS RVHQNLFLVGDPDQTIYEWRGAKPEYLVEFDSMFPDTQTIIMNRNYRSTPNILKIGNHIIKNNTVRVDKDMITDKPEGFE VVHFHGKNDFEESQWIASEIKEILQAEQAAYSDITILYRSNHLSRNIEQALIKDNIPYSIFGGIRFFERKEIKDVLSLLR LIVQGDNFSFLRMHNHPARGLGKKFLERLSLLAGEQNLSLLQALERNITDKELAKKGAVEFLELIAELRETAKTKSISDL VKIILDKSGLSELYRKDGDEDRLENIKELVNSMLLLEKENNAPVDIIEYLQEISLYTDLNTDTENQDKVKLMTIHISKGL EFPYVFLCGFTEGVLPSALSVKERRARAIEEERRLMYVAVTRAEKRFYMTDSEGFNFTTGLNKYPSRFLFEIKDEFYVRK GKLSQQIIENSRNHQSPGQLQENTAFKEGDFVMHPVWNKGKIQTVDLEKKEYLVDFFDMGKVKPIDFGFKHLTGFSEDSG DAEGPKELLDLAAHLKTAPDPFEQDTALKPQGITSLSSDSIFDSGPMPDLSENTKPDKKDSKKWWKL
Sequences:
>Translated_787_residues MNDPILENLNPSQLQAVKTTEGYVRVIAGAGSGKTKALTSRFAYIVDRLGINSSNILCVTFTNKAAQEMKKRVKALIGDT YDLAFITTYHGFCVRFLREEINKIHFPKNFIILDAEDQKSILRDIFNELQINSKHLTFKQVLRFISKQKSTSNYLGYILE NKGFEPDPSDSLSSRIFQIYLDKLKRNYALDFDDLIHFTAFILDHNPDVLLKWQQNLHYIQVDEAQDSSENQFHLVEILS RVHQNLFLVGDPDQTIYEWRGAKPEYLVEFDSMFPDTQTIIMNRNYRSTPNILKIGNHIIKNNTVRVDKDMITDKPEGFE VVHFHGKNDFEESQWIASEIKEILQAEQAAYSDITILYRSNHLSRNIEQALIKDNIPYSIFGGIRFFERKEIKDVLSLLR LIVQGDNFSFLRMHNHPARGLGKKFLERLSLLAGEQNLSLLQALERNITDKELAKKGAVEFLELIAELRETAKTKSISDL VKIILDKSGLSELYRKDGDEDRLENIKELVNSMLLLEKENNAPVDIIEYLQEISLYTDLNTDTENQDKVKLMTIHISKGL EFPYVFLCGFTEGVLPSALSVKERRARAIEEERRLMYVAVTRAEKRFYMTDSEGFNFTTGLNKYPSRFLFEIKDEFYVRK GKLSQQIIENSRNHQSPGQLQENTAFKEGDFVMHPVWNKGKIQTVDLEKKEYLVDFFDMGKVKPIDFGFKHLTGFSEDSG DAEGPKELLDLAAHLKTAPDPFEQDTALKPQGITSLSSDSIFDSGPMPDLSENTKPDKKDSKKWWKL >Mature_787_residues MNDPILENLNPSQLQAVKTTEGYVRVIAGAGSGKTKALTSRFAYIVDRLGINSSNILCVTFTNKAAQEMKKRVKALIGDT YDLAFITTYHGFCVRFLREEINKIHFPKNFIILDAEDQKSILRDIFNELQINSKHLTFKQVLRFISKQKSTSNYLGYILE NKGFEPDPSDSLSSRIFQIYLDKLKRNYALDFDDLIHFTAFILDHNPDVLLKWQQNLHYIQVDEAQDSSENQFHLVEILS RVHQNLFLVGDPDQTIYEWRGAKPEYLVEFDSMFPDTQTIIMNRNYRSTPNILKIGNHIIKNNTVRVDKDMITDKPEGFE VVHFHGKNDFEESQWIASEIKEILQAEQAAYSDITILYRSNHLSRNIEQALIKDNIPYSIFGGIRFFERKEIKDVLSLLR LIVQGDNFSFLRMHNHPARGLGKKFLERLSLLAGEQNLSLLQALERNITDKELAKKGAVEFLELIAELRETAKTKSISDL VKIILDKSGLSELYRKDGDEDRLENIKELVNSMLLLEKENNAPVDIIEYLQEISLYTDLNTDTENQDKVKLMTIHISKGL EFPYVFLCGFTEGVLPSALSVKERRARAIEEERRLMYVAVTRAEKRFYMTDSEGFNFTTGLNKYPSRFLFEIKDEFYVRK GKLSQQIIENSRNHQSPGQLQENTAFKEGDFVMHPVWNKGKIQTVDLEKKEYLVDFFDMGKVKPIDFGFKHLTGFSEDSG DAEGPKELLDLAAHLKTAPDPFEQDTALKPQGITSLSSDSIFDSGPMPDLSENTKPDKKDSKKWWKL
Specific function: Essential helicase [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI2367296, Length=697, Percent_Identity=33.142037302726, Blast_Score=376, Evalue=1e-105, Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=34.3260188087774, Blast_Score=328, Evalue=1e-90, Organism=Saccharomyces cerevisiae, GI6322369, Length=740, Percent_Identity=28.6486486486486, Blast_Score=190, Evalue=8e-49,
Paralogues:
None
Copy number: 3000 [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005751 - InterPro: IPR013986 - InterPro: IPR014017 - InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 90649; Mature: 90649
Theoretical pI: Translated: 6.06; Mature: 6.06
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNDPILENLNPSQLQAVKTTEGYVRVIAGAGSGKTKALTSRFAYIVDRLGINSSNILCVT CCCCCCCCCCHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE FTNKAAQEMKKRVKALIGDTYDLAFITTYHGFCVRFLREEINKIHFPKNFIILDAEDQKS ECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH ILRDIFNELQINSKHLTFKQVLRFISKQKSTSNYLGYILENKGFEPDPSDSLSSRIFQIY HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHH LDKLKRNYALDFDDLIHFTAFILDHNPDVLLKWQQNLHYIQVDEAQDSSENQFHLVEILS HHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHH RVHQNLFLVGDPDQTIYEWRGAKPEYLVEFDSMFPDTQTIIMNRNYRSTPNILKIGNHII HHHHCEEEEECCCCHHHHHCCCCCCEEEEEHHHCCCCEEEEEECCCCCCCCHHHHCCHHH KNNTVRVDKDMITDKPEGFEVVHFHGKNDFEESQWIASEIKEILQAEQAAYSDITILYRS CCCCEEECHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC NHLSRNIEQALIKDNIPYSIFGGIRFFERKEIKDVLSLLRLIVQGDNFSFLRMHNHPARG CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH LGKKFLERLSLLAGEQNLSLLQALERNITDKELAKKGAVEFLELIAELRETAKTKSISDL HHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH VKIILDKSGLSELYRKDGDEDRLENIKELVNSMLLLEKENNAPVDIIEYLQEISLYTDLN HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCC TDTENQDKVKLMTIHISKGLEFPYVFLCGFTEGVLPSALSVKERRARAIEEERRLMYVAV CCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEE TRAEKRFYMTDSEGFNFTTGLNKYPSRFLFEIKDEFYVRKGKLSQQIIENSRNHQSPGQL EECCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCH QENTAFKEGDFVMHPVWNKGKIQTVDLEKKEYLVDFFDMGKVKPIDFGFKHLTGFSEDSG HHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCC DAEGPKELLDLAAHLKTAPDPFEQDTALKPQGITSLSSDSIFDSGPMPDLSENTKPDKKD CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SKKWWKL CHHHCCC >Mature Secondary Structure MNDPILENLNPSQLQAVKTTEGYVRVIAGAGSGKTKALTSRFAYIVDRLGINSSNILCVT CCCCCCCCCCHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE FTNKAAQEMKKRVKALIGDTYDLAFITTYHGFCVRFLREEINKIHFPKNFIILDAEDQKS ECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH ILRDIFNELQINSKHLTFKQVLRFISKQKSTSNYLGYILENKGFEPDPSDSLSSRIFQIY HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHH LDKLKRNYALDFDDLIHFTAFILDHNPDVLLKWQQNLHYIQVDEAQDSSENQFHLVEILS HHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHH RVHQNLFLVGDPDQTIYEWRGAKPEYLVEFDSMFPDTQTIIMNRNYRSTPNILKIGNHII HHHHCEEEEECCCCHHHHHCCCCCCEEEEEHHHCCCCEEEEEECCCCCCCCHHHHCCHHH KNNTVRVDKDMITDKPEGFEVVHFHGKNDFEESQWIASEIKEILQAEQAAYSDITILYRS CCCCEEECHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC NHLSRNIEQALIKDNIPYSIFGGIRFFERKEIKDVLSLLRLIVQGDNFSFLRMHNHPARG CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH LGKKFLERLSLLAGEQNLSLLQALERNITDKELAKKGAVEFLELIAELRETAKTKSISDL HHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH VKIILDKSGLSELYRKDGDEDRLENIKELVNSMLLLEKENNAPVDIIEYLQEISLYTDLN HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCC TDTENQDKVKLMTIHISKGLEFPYVFLCGFTEGVLPSALSVKERRARAIEEERRLMYVAV CCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEE TRAEKRFYMTDSEGFNFTTGLNKYPSRFLFEIKDEFYVRKGKLSQQIIENSRNHQSPGQL EECCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCH QENTAFKEGDFVMHPVWNKGKIQTVDLEKKEYLVDFFDMGKVKPIDFGFKHLTGFSEDSG HHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCC DAEGPKELLDLAAHLKTAPDPFEQDTALKPQGITSLSSDSIFDSGPMPDLSENTKPDKKD CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SKKWWKL CHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA