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The map label for this gene is oprM [H]

Identifier: 146300971

GI number: 146300971

Start: 3842749

End: 3844158

Strand: Reverse

Name: oprM [H]

Synonym: Fjoh_3226

Alternate gene names: 146300971

Gene position: 3844158-3842749 (Counterclockwise)

Preceding gene: 146300972

Following gene: 146300970

Centisome position: 63.05

GC content: 38.37

Gene sequence:

>1410_bases
ATGAGAATACTATATAAAATTGGAATAACGGTATTTTCAGGTATGATTTTAACATCGTGCGTTGTTGGAAAAAAATATTC
CCGCACCGAGTTAAACGCTCCTGATAAATACCGAGAAGAATTAACCGTAACAGGAGATACTATTTTGCATTCCTGGAAAA
CGTATTACAAAGACCCGCTTTTAGTTAATTTGATCGAAAAAGCCCTCGTTAAAAACAACGAGGTGCTTATCGCGGTCAAA
AGCATGGAACAGCTGGATTTAAATTATAAACAAGCCAAACTGTCTTTACTGCCAACATTGGATTTTGATGCTGCAGCGAG
CCGTTCGTATTTGTCTAAAAATTCGTTAAACGGATCTTTGAGCGCGCAGTTTACGAGCAAAGATTATATGGACGATTACA
GCGCTAATCTTCGTATGTCGTGGGAAGTAGATATTTGGGGAAAAGCAACAATGCAGAAAAGAGATGCAAAAGCAGCGTAT
TTTGCCCAAAAGGAAAACCTTTCGGCTTTAAAAACCCGAATTATTGTTCAGGTGGCGCAGTCGTATTACAATCTTTTAGG
TTTAGACGAACAGCTTAAAATTGCTGAAAAAAACATCGAATTGAGCGATAATACCCTGCAGATGATGAAACTGCAGTACA
ACTCAGGTTCGATTAGTTCATTGGCAGTAAATCAGACTGAAGCTCAGAAAAAAACAGCAGAATTACTTGTTCCTTTGGCA
AAAACCAATATCGCGGTTCAGGAAAATGCACTGCAGATTTTATGCGGCGAATATCCTAATACGATCGAACGTTCAGGTAA
TTTAAATGTTGATGAATTTGCGTTTGAAATGCCATCTGGAGTTCCAGCTTCACTTTTAAGCCGAAGACCGGATGTAAAAT
CGAGTGAATATGCTGTGATGTCGGCAACAGCCAAAACGGGATTAGCAAAAGCTACAATGTATCCAACGCTGAGTTTAAGT
CCGTCAATTGGAATTAATTCATTTGAGTTCGATACCTGGTTTAATTTTCCGGGTTCGGTTACAAAAAATATAGCAGCAAA
TCTGGCACAGCCTATTTTTAGGAAAAAAGCCTTGCGAACTGCTTATGAAGTAGCGGTTTTAGAACAAGAAAAAGCTGTGG
TTCAGTTCAAACAATCGTTTATTACCGCTGTTGGTGAAGTTTCTGATGCCATGTCGAGATTAAAATATGCAGATGAACGC
ATTAAGCTGGCAGAAGAAAAAGCAATATCTTTAGAAAAAGCAACTTCAGATGCTTCACTTTTATACAAAAGCGGTATGGC
CAATTATCTTGAAATTATTGCCGCTCAAAATAGTGCCTTGCAAAACGATCTTGATGTCGTAACAATAAAATTAGAAAAAT
TAAATGCCTCTATTAATTTGTACCGCGCTTTGGGCGGAGGAGTAGAGTAG

Upstream 100 bases:

>100_bases
TAGGAGTTTTTGTGATTCCGCTGCTTTTCATTGTATTTCAATATCTGCAGGAAAAAGTATCAGGTAAGAAGAATGTAGTT
CAAACCGTAAAAGAATAATA

Downstream 100 bases:

>100_bases
TGCTGCATTTCTAAATCCATCATCTTTATAAATGTAAAGATGGTGGATTTTGTATATTATTTTGATGGTTTAAACTGTCT
TGTTTTAATATGGACAAAAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRILYKIGITVFSGMILTSCVVGKKYSRTELNAPDKYREELTVTGDTILHSWKTYYKDPLLVNLIEKALVKNNEVLIAVK
SMEQLDLNYKQAKLSLLPTLDFDAAASRSYLSKNSLNGSLSAQFTSKDYMDDYSANLRMSWEVDIWGKATMQKRDAKAAY
FAQKENLSALKTRIIVQVAQSYYNLLGLDEQLKIAEKNIELSDNTLQMMKLQYNSGSISSLAVNQTEAQKKTAELLVPLA
KTNIAVQENALQILCGEYPNTIERSGNLNVDEFAFEMPSGVPASLLSRRPDVKSSEYAVMSATAKTGLAKATMYPTLSLS
PSIGINSFEFDTWFNFPGSVTKNIAANLAQPIFRKKALRTAYEVAVLEQEKAVVQFKQSFITAVGEVSDAMSRLKYADER
IKLAEEKAISLEKATSDASLLYKSGMANYLEIIAAQNSALQNDLDVVTIKLEKLNASINLYRALGGGVE

Sequences:

>Translated_469_residues
MRILYKIGITVFSGMILTSCVVGKKYSRTELNAPDKYREELTVTGDTILHSWKTYYKDPLLVNLIEKALVKNNEVLIAVK
SMEQLDLNYKQAKLSLLPTLDFDAAASRSYLSKNSLNGSLSAQFTSKDYMDDYSANLRMSWEVDIWGKATMQKRDAKAAY
FAQKENLSALKTRIIVQVAQSYYNLLGLDEQLKIAEKNIELSDNTLQMMKLQYNSGSISSLAVNQTEAQKKTAELLVPLA
KTNIAVQENALQILCGEYPNTIERSGNLNVDEFAFEMPSGVPASLLSRRPDVKSSEYAVMSATAKTGLAKATMYPTLSLS
PSIGINSFEFDTWFNFPGSVTKNIAANLAQPIFRKKALRTAYEVAVLEQEKAVVQFKQSFITAVGEVSDAMSRLKYADER
IKLAEEKAISLEKATSDASLLYKSGMANYLEIIAAQNSALQNDLDVVTIKLEKLNASINLYRALGGGVE
>Mature_469_residues
MRILYKIGITVFSGMILTSCVVGKKYSRTELNAPDKYREELTVTGDTILHSWKTYYKDPLLVNLIEKALVKNNEVLIAVK
SMEQLDLNYKQAKLSLLPTLDFDAAASRSYLSKNSLNGSLSAQFTSKDYMDDYSANLRMSWEVDIWGKATMQKRDAKAAY
FAQKENLSALKTRIIVQVAQSYYNLLGLDEQLKIAEKNIELSDNTLQMMKLQYNSGSISSLAVNQTEAQKKTAELLVPLA
KTNIAVQENALQILCGEYPNTIERSGNLNVDEFAFEMPSGVPASLLSRRPDVKSSEYAVMSATAKTGLAKATMYPTLSLS
PSIGINSFEFDTWFNFPGSVTKNIAANLAQPIFRKKALRTAYEVAVLEQEKAVVQFKQSFITAVGEVSDAMSRLKYADER
IKLAEEKAISLEKATSDASLLYKSGMANYLEIIAAQNSALQNDLDVVTIKLEKLNASINLYRALGGGVE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=25.764192139738, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI2367348, Length=472, Percent_Identity=20.7627118644068, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52015; Mature: 52015

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILYKIGITVFSGMILTSCVVGKKYSRTELNAPDKYREELTVTGDTILHSWKTYYKDPL
CEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEECHHHHHHHHHHHHCCCH
LVNLIEKALVKNNEVLIAVKSMEQLDLNYKQAKLSLLPTLDFDAAASRSYLSKNSLNGSL
HHHHHHHHHHCCCCEEEEEECHHHHCCCHHHHEEEEECCCCCCHHHHHHHHHHCCCCCCE
SAQFTSKDYMDDYSANLRMSWEVDIWGKATMQKRDAKAAYFAQKENLSALKTRIIVQVAQ
EEEECCCHHCCCCCCCEEEEEEEEECCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHH
SYYNLLGLDEQLKIAEKNIELSDNTLQMMKLQYNSGSISSLAVNQTEAQKKTAELLVPLA
HHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHH
KTNIAVQENALQILCGEYPNTIERSGNLNVDEFAFEMPSGVPASLLSRRPDVKSSEYAVM
HCCEEEECCCHHEEECCCCCHHHCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEE
SATAKTGLAKATMYPTLSLSPSIGINSFEFDTWFNFPGSVTKNIAANLAQPIFRKKALRT
EEHHHCCCHHHEECCEEECCCCCCCCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
AYEVAVLEQEKAVVQFKQSFITAVGEVSDAMSRLKYADERIKLAEEKAISLEKATSDASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LYKSGMANYLEIIAAQNSALQNDLDVVTIKLEKLNASINLYRALGGGVE
HHHHCHHHHHHHHHHCCHHHCCCCEEEEEEEEECCCCEEEEECCCCCCC
>Mature Secondary Structure
MRILYKIGITVFSGMILTSCVVGKKYSRTELNAPDKYREELTVTGDTILHSWKTYYKDPL
CEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEECHHHHHHHHHHHHCCCH
LVNLIEKALVKNNEVLIAVKSMEQLDLNYKQAKLSLLPTLDFDAAASRSYLSKNSLNGSL
HHHHHHHHHHCCCCEEEEEECHHHHCCCHHHHEEEEECCCCCCHHHHHHHHHHCCCCCCE
SAQFTSKDYMDDYSANLRMSWEVDIWGKATMQKRDAKAAYFAQKENLSALKTRIIVQVAQ
EEEECCCHHCCCCCCCEEEEEEEEECCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHH
SYYNLLGLDEQLKIAEKNIELSDNTLQMMKLQYNSGSISSLAVNQTEAQKKTAELLVPLA
HHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHH
KTNIAVQENALQILCGEYPNTIERSGNLNVDEFAFEMPSGVPASLLSRRPDVKSSEYAVM
HCCEEEECCCHHEEECCCCCHHHCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEE
SATAKTGLAKATMYPTLSLSPSIGINSFEFDTWFNFPGSVTKNIAANLAQPIFRKKALRT
EEHHHCCCHHHEECCEEECCCCCCCCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
AYEVAVLEQEKAVVQFKQSFITAVGEVSDAMSRLKYADERIKLAEEKAISLEKATSDASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LYKSGMANYLEIIAAQNSALQNDLDVVTIKLEKLNASINLYRALGGGVE
HHHHCHHHHHHHHHHCCHHHCCCCEEEEEEEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]