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The map label for this gene is eptA [H]

Identifier: 146300795

GI number: 146300795

Start: 3610835

End: 3612355

Strand: Direct

Name: eptA [H]

Synonym: Fjoh_3047

Alternate gene names: 146300795

Gene position: 3610835-3612355 (Clockwise)

Preceding gene: 146300794

Following gene: 146300796

Centisome position: 59.22

GC content: 51.28

Gene sequence:

>1521_bases
ATGTTAAAAGGTAGTCTTCCGGCAGCCCGTCTGGCGGCTTTAGCAAGCCTGGCCAACTGCATATTTTTCCATATCCCCTT
CTTCGCTTTTGTGGTCGGGAACCTCAATTACGCGGGATTCAGCGGCATGATGACCATAGCCAGCCTCATCCTGCTGATGC
TTGTGCTGGATTTCCTGGTCTTCTATCTGCTGGTCTACCTTGCCGGACTTATCGGCAGAATTATCATTTCGCTTTTTTTT
GCCCTCAGCGCGGCGGCCCTGTATTTTATCAATGCCTACAGCGTGATCATAGACGAAAGCATGATCGGGAATATCCTCAA
CACCAATTATGCGGAATCAAGCGCCTATTTTTCTATCACCCTGGCCCTCTATGTGCTCGTGCTCGGGATACTTCCCTCGG
CGGCGCTCTTGAGGATCAGGACCGTAAAAGAGCCCCTTAAAAGGTTTGCAGCGCTCTGTTCGGGAAGCTTTCTCATTGCG
CTTGCACTGGTTTTTATCAATTCTGCCGGCTGGCTTTGGATCGACAAGAATTCCAAAAAATTGGGAGGCCTGGCAATGCC
TTGGTCCTATGCGGTCAACACCACTCTTTTCTACGTGCACCAGTATAAGAAAAACCAGAAGGAAATCCTTCTTCCCGATG
CCCGCATCAGCAGCAGGAAGAAATCCATTGTGGTGCTGGTCATCGGGGAATCCGCAAGAAAGGCCAATTTCTCCCTGTAC
GGCTATCCGAAGAACACCAATCCGATGCTTGCAGACATAGAAAATGTGCACCCTTTGGACGCGGTTTCCGCCGCAACCTA
CACCACGGCCGGGGTAAAGGCGATCCTGGATTACAAAAAGACGGATGAGCTGTACGAAATACTGCCCAACTACCTCTTCC
GGAACAATGTGGAGGTCATATGGAGGACCGCCAACTGGGGAGAGCCGCCGCTGCACATCAAAAATTACCAGGACAGGAAA
TACCTTGGCGCAGCCTGCAGGGGAAAAGCCTGCGGCTATGACGAAATCCTGCTGGCGCTTTTCAAAGAGCAGATACTGGC
CAGCAGAAAAGACAAGATACTCCTGGTGCTGCATACCAGCACCAGCCACGGCCCGAATTACAGCGCCAAGTATCCGGCAG
GCTTCCGGAAGTTCAGTCCGGTCTGTAGCAGCGTCGAGCTTGCCGACTGCTCAAAGCAGGAACTGATGAACGCCTACGAC
AATACGATAGTCTATACCGATTACCTGCTCAGCCGCGTGATCCAGAACCTGAAAGAGCTCAAGGAATTTGACAGCACCAT
GATTTTTGTTTCCGACCACGGAGAGTCCCTCGGGGAAAACAATCTCTACATGCACGGCCTTCCGATGAGCATTGCCCCGA
AGGAGCAGTTCGAGATACCCTTTATTGTCTGGGCATCGCCCGGCTCAAAAAAGCTGAAGGCCAATAAAACGGTGACCCAG
TACCATGTCTTTCACAGCGTGCTGAACTTTCTGGATGTGGAAAGCCCCATCTACGACGAAAATATGAACATTTACCGATA
G

Upstream 100 bases:

>100_bases
TGGCTTGCGCTGGCCGGAAAGATCCTGCAGGAGAACCTATTGGTATTTTACATCTCAATTGCCGCATTATCCGTATCTTT
ATTTAAAATAACCGCCGCAT

Downstream 100 bases:

>100_bases
GATCAACAACCGGCCTCGGCCATAAAAATAATTTCATATGAATGGAACAGTATTTATCAACCGGCTCAGCAACAGGTCAA
GGCTGGCAATTTCCGCTTCG

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLKGSLPAARLAALASLANCIFFHIPFFAFVVGNLNYAGFSGMMTIASLILLMLVLDFLVFYLLVYLAGLIGRIIISLFF
ALSAAALYFINAYSVIIDESMIGNILNTNYAESSAYFSITLALYVLVLGILPSAALLRIRTVKEPLKRFAALCSGSFLIA
LALVFINSAGWLWIDKNSKKLGGLAMPWSYAVNTTLFYVHQYKKNQKEILLPDARISSRKKSIVVLVIGESARKANFSLY
GYPKNTNPMLADIENVHPLDAVSAATYTTAGVKAILDYKKTDELYEILPNYLFRNNVEVIWRTANWGEPPLHIKNYQDRK
YLGAACRGKACGYDEILLALFKEQILASRKDKILLVLHTSTSHGPNYSAKYPAGFRKFSPVCSSVELADCSKQELMNAYD
NTIVYTDYLLSRVIQNLKELKEFDSTMIFVSDHGESLGENNLYMHGLPMSIAPKEQFEIPFIVWASPGSKKLKANKTVTQ
YHVFHSVLNFLDVESPIYDENMNIYR

Sequences:

>Translated_506_residues
MLKGSLPAARLAALASLANCIFFHIPFFAFVVGNLNYAGFSGMMTIASLILLMLVLDFLVFYLLVYLAGLIGRIIISLFF
ALSAAALYFINAYSVIIDESMIGNILNTNYAESSAYFSITLALYVLVLGILPSAALLRIRTVKEPLKRFAALCSGSFLIA
LALVFINSAGWLWIDKNSKKLGGLAMPWSYAVNTTLFYVHQYKKNQKEILLPDARISSRKKSIVVLVIGESARKANFSLY
GYPKNTNPMLADIENVHPLDAVSAATYTTAGVKAILDYKKTDELYEILPNYLFRNNVEVIWRTANWGEPPLHIKNYQDRK
YLGAACRGKACGYDEILLALFKEQILASRKDKILLVLHTSTSHGPNYSAKYPAGFRKFSPVCSSVELADCSKQELMNAYD
NTIVYTDYLLSRVIQNLKELKEFDSTMIFVSDHGESLGENNLYMHGLPMSIAPKEQFEIPFIVWASPGSKKLKANKTVTQ
YHVFHSVLNFLDVESPIYDENMNIYR
>Mature_506_residues
MLKGSLPAARLAALASLANCIFFHIPFFAFVVGNLNYAGFSGMMTIASLILLMLVLDFLVFYLLVYLAGLIGRIIISLFF
ALSAAALYFINAYSVIIDESMIGNILNTNYAESSAYFSITLALYVLVLGILPSAALLRIRTVKEPLKRFAALCSGSFLIA
LALVFINSAGWLWIDKNSKKLGGLAMPWSYAVNTTLFYVHQYKKNQKEILLPDARISSRKKSIVVLVIGESARKANFSLY
GYPKNTNPMLADIENVHPLDAVSAATYTTAGVKAILDYKKTDELYEILPNYLFRNNVEVIWRTANWGEPPLHIKNYQDRK
YLGAACRGKACGYDEILLALFKEQILASRKDKILLVLHTSTSHGPNYSAKYPAGFRKFSPVCSSVELADCSKQELMNAYD
NTIVYTDYLLSRVIQNLKELKEFDSTMIFVSDHGESLGENNLYMHGLPMSIAPKEQFEIPFIVWASPGSKKLKANKTVTQ
YHVFHSVLNFLDVESPIYDENMNIYR

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=525, Percent_Identity=31.047619047619, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87082286, Length=479, Percent_Identity=24.6346555323591, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790392, Length=487, Percent_Identity=25.2566735112936, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082223, Length=313, Percent_Identity=26.517571884984, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 56691; Mature: 56691

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGSLPAARLAALASLANCIFFHIPFFAFVVGNLNYAGFSGMMTIASLILLMLVLDFLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FYLLVYLAGLIGRIIISLFFALSAAALYFINAYSVIIDESMIGNILNTNYAESSAYFSIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LALYVLVLGILPSAALLRIRTVKEPLKRFAALCSGSFLIALALVFINSAGWLWIDKNSKK
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCC
LGGLAMPWSYAVNTTLFYVHQYKKNQKEILLPDARISSRKKSIVVLVIGESARKANFSLY
CCCCCCCHHHHHHHEEEEEEHHCCCCCEEECCCCHHCCCCCEEEEEEECCCCCCCCEEEE
GYPKNTNPMLADIENVHPLDAVSAATYTTAGVKAILDYKKTDELYEILPNYLFRNNVEVI
ECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WRTANWGEPPLHIKNYQDRKYLGAACRGKACGYDEILLALFKEQILASRKDKILLVLHTS
EEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
TSHGPNYSAKYPAGFRKFSPVCSSVELADCSKQELMNAYDNTIVYTDYLLSRVIQNLKEL
CCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
KEFDSTMIFVSDHGESLGENNLYMHGLPMSIAPKEQFEIPFIVWASPGSKKLKANKTVTQ
HHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHH
YHVFHSVLNFLDVESPIYDENMNIYR
HHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKGSLPAARLAALASLANCIFFHIPFFAFVVGNLNYAGFSGMMTIASLILLMLVLDFLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FYLLVYLAGLIGRIIISLFFALSAAALYFINAYSVIIDESMIGNILNTNYAESSAYFSIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LALYVLVLGILPSAALLRIRTVKEPLKRFAALCSGSFLIALALVFINSAGWLWIDKNSKK
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCC
LGGLAMPWSYAVNTTLFYVHQYKKNQKEILLPDARISSRKKSIVVLVIGESARKANFSLY
CCCCCCCHHHHHHHEEEEEEHHCCCCCEEECCCCHHCCCCCEEEEEEECCCCCCCCEEEE
GYPKNTNPMLADIENVHPLDAVSAATYTTAGVKAILDYKKTDELYEILPNYLFRNNVEVI
ECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WRTANWGEPPLHIKNYQDRKYLGAACRGKACGYDEILLALFKEQILASRKDKILLVLHTS
EEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
TSHGPNYSAKYPAGFRKFSPVCSSVELADCSKQELMNAYDNTIVYTDYLLSRVIQNLKEL
CCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
KEFDSTMIFVSDHGESLGENNLYMHGLPMSIAPKEQFEIPFIVWASPGSKKLKANKTVTQ
HHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHH
YHVFHSVLNFLDVESPIYDENMNIYR
HHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]