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The map label for this gene is pit [H]

Identifier: 146300333

GI number: 146300333

Start: 3067210

End: 3068544

Strand: Direct

Name: pit [H]

Synonym: Fjoh_2578

Alternate gene names: 146300333

Gene position: 3067210-3068544 (Clockwise)

Preceding gene: 146300332

Following gene: 146300334

Centisome position: 50.31

GC content: 37.23

Gene sequence:

>1335_bases
ATGACGCTACTTATATTAATTATAGTATTAGCCTTAATTTTTGATTACATCAATGGTTTTCATGATGCGGCAAATGCTAT
AGCGACTGTTGTTGCAACAAAGGTTTTAACACCTTTTCAGGCGGTTGTCTGGGCAGCATTTTTTAACTTTTTGGCTTATT
GGGTTTTCGGATTTGGTGTTGCAGATACTGTTGCCAAAACTGCGCACACCATGGAAATTAACCTGGTTGTAATTCTCGCT
GGAGTAATTGCAGCAATCTGCTGGAACTTATTGACTTGGTGGTTAGGAATTCCTTCAAGTTCTTCACATACATTAATTGG
AGGTTTTGCCGGAGCAGCTGTTGCACACGCAATCGCAGTTCACGGTTTTTCTGGTTATGTTGGAGAAGACGGTGCAACAC
ATTATTGGTATGAAATTGTAAGCTGGTATAAGGCAGGAAAAGATGGCGCAATGCCCTCCGGAGTCCTTATTATTATTGCT
TTTATAGTTCTTGCGCCATTATTAGGAGCTTTGGCTTCATATTTAATTTCTATCTGGTTATTAAATGCTTCTCGAAAAAG
TATTGGACCTAAAATTTTTACTGGAGCATTAATGATTGCTACAATCGTATTTGTATACTATCAGATGATTCCTTATTCAG
AGATTGATAAACCAAGATTTGATTCTCATTTTTGGAGTGTTGCTTTTGAAGCCCACAATATTAAATGGTTTTTAGTTGCT
TTCATTGTCTTGACAGTTAGTGGTTTTTGTATGATATTCAGTAGTTTGAATCTTCATCAGGCTGATGCTGCTTTGAAAAA
AATGCAATTATTATCTTCTGCGGCGTTTAGTTTAGGACACGGAGGAAATGATTCTCAAAAAGTAATGGGAATTATTGCTG
CAGCTGTTGCTGTTTATATCAATACTAATCACGGTGTACATATGGATGCCTGGTTAGATGTTGTTCTGCCAAATGAAGAT
TTAGGAATTAAAGGACAAATGCCAAGCTGGATTCCGTTAGCATGTTATTCTGCAATTGCAGCCGGAACCTTAAGCGGTGG
ATGGAAAATTGTAAAAACAATGGGATCTAAAATCACAAAAGTAAGTTCGTTTGAAGGTGTTGCCGCTGAAACTGCAGGAG
CTTTGACACTTTACTTTACAGAGCATTTAAAAATCCCGGTAAGTACAACGCATACTATTACAGGATCTATTATTGGTGTT
GGATTAACAAAACGTGTTTCTGCAGTTCGCTGGGGAGTTACTGTAAGTTTAGTCTGGGCCTGGATTTTGACTATTCCAAT
TTCGGCTATATTAGCAGGTTTGGTTTATTTTATACTAAGTGTATTTATTTCATAA

Upstream 100 bases:

>100_bases
AGAATCAGCAACAGATAAATGTAAAAGTGTTGCAAACATACTAGAATCTATTTCTGTAAAACATTCTTAATTCAATTTTT
CAATCTGAAGTTATAACTTT

Downstream 100 bases:

>100_bases
TATGAGTATTGCAAAATGTGATATATAATCACATTTTAAAAAATATCTTAAAGCCGATTTCAATTTGAAATCGGCTTTTT
TTGTTTGTATTTATAAATGA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTLLILIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAVVWAAFFNFLAYWVFGFGVADTVAKTAHTMEINLVVILA
GVIAAICWNLLTWWLGIPSSSSHTLIGGFAGAAVAHAIAVHGFSGYVGEDGATHYWYEIVSWYKAGKDGAMPSGVLIIIA
FIVLAPLLGALASYLISIWLLNASRKSIGPKIFTGALMIATIVFVYYQMIPYSEIDKPRFDSHFWSVAFEAHNIKWFLVA
FIVLTVSGFCMIFSSLNLHQADAALKKMQLLSSAAFSLGHGGNDSQKVMGIIAAAVAVYINTNHGVHMDAWLDVVLPNED
LGIKGQMPSWIPLACYSAIAAGTLSGGWKIVKTMGSKITKVSSFEGVAAETAGALTLYFTEHLKIPVSTTHTITGSIIGV
GLTKRVSAVRWGVTVSLVWAWILTIPISAILAGLVYFILSVFIS

Sequences:

>Translated_444_residues
MTLLILIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAVVWAAFFNFLAYWVFGFGVADTVAKTAHTMEINLVVILA
GVIAAICWNLLTWWLGIPSSSSHTLIGGFAGAAVAHAIAVHGFSGYVGEDGATHYWYEIVSWYKAGKDGAMPSGVLIIIA
FIVLAPLLGALASYLISIWLLNASRKSIGPKIFTGALMIATIVFVYYQMIPYSEIDKPRFDSHFWSVAFEAHNIKWFLVA
FIVLTVSGFCMIFSSLNLHQADAALKKMQLLSSAAFSLGHGGNDSQKVMGIIAAAVAVYINTNHGVHMDAWLDVVLPNED
LGIKGQMPSWIPLACYSAIAAGTLSGGWKIVKTMGSKITKVSSFEGVAAETAGALTLYFTEHLKIPVSTTHTITGSIIGV
GLTKRVSAVRWGVTVSLVWAWILTIPISAILAGLVYFILSVFIS
>Mature_443_residues
TLLILIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAVVWAAFFNFLAYWVFGFGVADTVAKTAHTMEINLVVILAG
VIAAICWNLLTWWLGIPSSSSHTLIGGFAGAAVAHAIAVHGFSGYVGEDGATHYWYEIVSWYKAGKDGAMPSGVLIIIAF
IVLAPLLGALASYLISIWLLNASRKSIGPKIFTGALMIATIVFVYYQMIPYSEIDKPRFDSHFWSVAFEAHNIKWFLVAF
IVLTVSGFCMIFSSLNLHQADAALKKMQLLSSAAFSLGHGGNDSQKVMGIIAAAVAVYINTNHGVHMDAWLDVVLPNEDL
GIKGQMPSWIPLACYSAIAAGTLSGGWKIVKTMGSKITKVSSFEGVAAETAGALTLYFTEHLKIPVSTTHTITGSIIGVG
LTKRVSAVRWGVTVSLVWAWILTIPISAILAGLVYFILSVFIS

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=176, Percent_Identity=28.9772727272727, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5803173, Length=177, Percent_Identity=28.8135593220339, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789360, Length=112, Percent_Identity=42.8571428571429, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789907, Length=112, Percent_Identity=42.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539280, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25146401, Length=173, Percent_Identity=23.6994219653179, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71981576, Length=173, Percent_Identity=21.3872832369942, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536725, Length=173, Percent_Identity=27.7456647398844, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=169, Percent_Identity=29.585798816568, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 47727; Mature: 47595

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLILIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAVVWAAFFNFLAYWVFGFGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADTVAKTAHTMEINLVVILAGVIAAICWNLLTWWLGIPSSSSHTLIGGFAGAAVAHAIAV
HHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
HGFSGYVGEDGATHYWYEIVSWYKAGKDGAMPSGVLIIIAFIVLAPLLGALASYLISIWL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNASRKSIGPKIFTGALMIATIVFVYYQMIPYSEIDKPRFDSHFWSVAFEAHNIKWFLVA
HCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCHHHHHH
FIVLTVSGFCMIFSSLNLHQADAALKKMQLLSSAAFSLGHGGNDSQKVMGIIAAAVAVYI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
NTNHGVHMDAWLDVVLPNEDLGIKGQMPSWIPLACYSAIAAGTLSGGWKIVKTMGSKITK
ECCCCEEEHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VSSFEGVAAETAGALTLYFTEHLKIPVSTTHTITGSIIGVGLTKRVSAVRWGVTVSLVWA
HCCCCCCHHHCCCEEEEEEEEEEEECCCCCCEEECHHEECCHHHHHHHHHHHHHHHHHHH
WILTIPISAILAGLVYFILSVFIS
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLLILIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAVVWAAFFNFLAYWVFGFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADTVAKTAHTMEINLVVILAGVIAAICWNLLTWWLGIPSSSSHTLIGGFAGAAVAHAIAV
HHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
HGFSGYVGEDGATHYWYEIVSWYKAGKDGAMPSGVLIIIAFIVLAPLLGALASYLISIWL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNASRKSIGPKIFTGALMIATIVFVYYQMIPYSEIDKPRFDSHFWSVAFEAHNIKWFLVA
HCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCHHHHHH
FIVLTVSGFCMIFSSLNLHQADAALKKMQLLSSAAFSLGHGGNDSQKVMGIIAAAVAVYI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
NTNHGVHMDAWLDVVLPNEDLGIKGQMPSWIPLACYSAIAAGTLSGGWKIVKTMGSKITK
ECCCCEEEHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VSSFEGVAAETAGALTLYFTEHLKIPVSTTHTITGSIIGVGLTKRVSAVRWGVTVSLVWA
HCCCCCCHHHCCCEEEEEEEEEEEECCCCCCEEECHHEECCHHHHHHHHHHHHHHHHHHH
WILTIPISAILAGLVYFILSVFIS
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]