Click here to switch to the map view.

The map label for this gene is thrC [H]

Identifier: 146300328

GI number: 146300328

Start: 3059408

End: 3060697

Strand: Reverse

Name: thrC [H]

Synonym: Fjoh_2573

Alternate gene names: 146300328

Gene position: 3060697-3059408 (Counterclockwise)

Preceding gene: 146300329

Following gene: 146300325

Centisome position: 50.2

GC content: 38.22

Gene sequence:

>1290_bases
ATGAAATACTACAGTTTAAACCATAATGCCCCAAAAGTTTCTTTTCAGGAAGCTGTTATACAAGGATTAGCAACTGACAA
AGGATTATATTTCCCAGAAAGCATTACACCGCTTGATCCTTCATTTTTTGATAAAATCGAAAGTTTAAGCCATGAAGAAA
TTGCTTTTGAAGCGATTAAACAATTTGTTGGCGACGAAATTCCAGCAGAAAAACTAAAAGAAATCATTGCTGAAACTTTA
GTTTTTGATTTTCCAGTTGTGAAAGTCGAAAACGGAATCTATTCGTTAGAATTATTTCACGGGCCGACAATGGCGTTTAA
AGACGTTGGAGCGCGTTTTATGTCGCGTTGTCTGGGCTATTTCAATAAAGACAAAAAAGACGCTAAAAACACCGTTTTAG
TAGCTACATCCGGAGATACAGGCGGAGCTGTTGCAAGCGGATTTCTTGGCGTTGACGGCGTTGATGTTGTCATTTTATAT
CCGTCAGGAAAAGTGAGCGATATTCAGGAAAAACAATTGACGACTTTAGGGCAAAACATTAAAGCACTTGAAGTTGACGG
TGTTTTTGACGATTGTCAGGATATGGTGAAAAAAGCGTTTTTAGACGAAACTTTAGCGCATAAAAACCTGACTTCGGCTA
ACTCTATTAATATTGCGCGCTGGTTACCGCAAATGTTTTATTTCTTCTTTGCTTACAAAGCATTAAAAAGCCAAAACAAA
CCTTTAGTTTTCTCTTGCCCAAGCGGAAACTTCGGGAATATCTGCGCCGGAATTATGGCAAAAAAATTAGGTTTGCCGAT
TGAACATTTCGTTGCGTCTACAAACGTAAACGACACCGTGCCAAGATTCTTAGAAAACGGAAAATACGACCCGAAACCTT
CTAAAGCAACAATCTCTAACGCCATGGACGTTGGAAACCCAAGCAACTTTATTAGAATTCAGGAATTGTACAATAATGAC
TTAAAAGCTTTCGAAAAAGATTTCTCTTCTTATAGTTATACCGATGAAGAAACGCTTGAAGCGCTAAAGAAAATCTATAA
CGCAGATGGTTACATTGCAGAACCGCACGGCGCTGTTGGTTATTTAGGTTTGAAAAAAGAACTAGAAAAACACCCCAACG
CAATTGGTGTTTTCTTAGAAACAGCGCATCCTATTAAATTCTTAGATGTTGTTGAACCGGCGCTAGGAATTACACTTCCT
ATTCCTGCTCAAATTGAGAGTGTTATTAATGAGGAGAAAGTTAGTGTGAAGATTAGGACTTATGAGGAGTTGAAGAGTTT
CTTGGGGTAG

Upstream 100 bases:

>100_bases
ATTTAAAATGAGTGCTTAGTTCCTAGTCCTTAGTAATTAGTCGCAAAAGACTAAGACTAGCAGCTAAGGACTAAGGACTA
ATTACTAAGAACTAATAAAA

Downstream 100 bases:

>100_bases
TAAAAAATAAAAGACAGCTATAATTATTTTTAGCTGTCTTTTATTTTCACATCTACATAAAATCATTTAAAGTCAATTCA
TAGTCAATTATTATGTTTTT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKYYSLNHNAPKVSFQEAVIQGLATDKGLYFPESITPLDPSFFDKIESLSHEEIAFEAIKQFVGDEIPAEKLKEIIAETL
VFDFPVVKVENGIYSLELFHGPTMAFKDVGARFMSRCLGYFNKDKKDAKNTVLVATSGDTGGAVASGFLGVDGVDVVILY
PSGKVSDIQEKQLTTLGQNIKALEVDGVFDDCQDMVKKAFLDETLAHKNLTSANSINIARWLPQMFYFFFAYKALKSQNK
PLVFSCPSGNFGNICAGIMAKKLGLPIEHFVASTNVNDTVPRFLENGKYDPKPSKATISNAMDVGNPSNFIRIQELYNND
LKAFEKDFSSYSYTDEETLEALKKIYNADGYIAEPHGAVGYLGLKKELEKHPNAIGVFLETAHPIKFLDVVEPALGITLP
IPAQIESVINEEKVSVKIRTYEELKSFLG

Sequences:

>Translated_429_residues
MKYYSLNHNAPKVSFQEAVIQGLATDKGLYFPESITPLDPSFFDKIESLSHEEIAFEAIKQFVGDEIPAEKLKEIIAETL
VFDFPVVKVENGIYSLELFHGPTMAFKDVGARFMSRCLGYFNKDKKDAKNTVLVATSGDTGGAVASGFLGVDGVDVVILY
PSGKVSDIQEKQLTTLGQNIKALEVDGVFDDCQDMVKKAFLDETLAHKNLTSANSINIARWLPQMFYFFFAYKALKSQNK
PLVFSCPSGNFGNICAGIMAKKLGLPIEHFVASTNVNDTVPRFLENGKYDPKPSKATISNAMDVGNPSNFIRIQELYNND
LKAFEKDFSSYSYTDEETLEALKKIYNADGYIAEPHGAVGYLGLKKELEKHPNAIGVFLETAHPIKFLDVVEPALGITLP
IPAQIESVINEEKVSVKIRTYEELKSFLG
>Mature_429_residues
MKYYSLNHNAPKVSFQEAVIQGLATDKGLYFPESITPLDPSFFDKIESLSHEEIAFEAIKQFVGDEIPAEKLKEIIAETL
VFDFPVVKVENGIYSLELFHGPTMAFKDVGARFMSRCLGYFNKDKKDAKNTVLVATSGDTGGAVASGFLGVDGVDVVILY
PSGKVSDIQEKQLTTLGQNIKALEVDGVFDDCQDMVKKAFLDETLAHKNLTSANSINIARWLPQMFYFFFAYKALKSQNK
PLVFSCPSGNFGNICAGIMAKKLGLPIEHFVASTNVNDTVPRFLENGKYDPKPSKATISNAMDVGNPSNFIRIQELYNND
LKAFEKDFSSYSYTDEETLEALKKIYNADGYIAEPHGAVGYLGLKKELEKHPNAIGVFLETAHPIKFLDVVEPALGITLP
IPAQIESVINEEKVSVKIRTYEELKSFLG

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=458, Percent_Identity=32.0960698689956, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI153792148, Length=435, Percent_Identity=32.6436781609195, Blast_Score=178, Evalue=7e-45,
Organism=Escherichia coli, GI1786185, Length=407, Percent_Identity=46.4373464373464, Blast_Score=347, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6319901, Length=456, Percent_Identity=40.5701754385965, Blast_Score=263, Evalue=3e-71,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 47497; Mature: 47497

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50044 SIGMA54_3 ; PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYSLNHNAPKVSFQEAVIQGLATDKGLYFPESITPLDPSFFDKIESLSHEEIAFEAIK
CCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
QFVGDEIPAEKLKEIIAETLVFDFPVVKVENGIYSLELFHGPTMAFKDVGARFMSRCLGY
HHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
FNKDKKDAKNTVLVATSGDTGGAVASGFLGVDGVDVVILYPSGKVSDIQEKQLTTLGQNI
HCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
KALEVDGVFDDCQDMVKKAFLDETLAHKNLTSANSINIARWLPQMFYFFFAYKALKSQNK
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PLVFSCPSGNFGNICAGIMAKKLGLPIEHFVASTNVNDTVPRFLENGKYDPKPSKATISN
CEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHH
AMDVGNPSNFIRIQELYNNDLKAFEKDFSSYSYTDEETLEALKKIYNADGYIAEPHGAVG
HHCCCCCCCCEEHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCCCEE
YLGLKKELEKHPNAIGVFLETAHPIKFLDVVEPALGITLPIPAQIESVINEEKVSVKIRT
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEECCCHHHHHHHHCCHHEEEEEEE
YEELKSFLG
HHHHHHHCC
>Mature Secondary Structure
MKYYSLNHNAPKVSFQEAVIQGLATDKGLYFPESITPLDPSFFDKIESLSHEEIAFEAIK
CCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
QFVGDEIPAEKLKEIIAETLVFDFPVVKVENGIYSLELFHGPTMAFKDVGARFMSRCLGY
HHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
FNKDKKDAKNTVLVATSGDTGGAVASGFLGVDGVDVVILYPSGKVSDIQEKQLTTLGQNI
HCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
KALEVDGVFDDCQDMVKKAFLDETLAHKNLTSANSINIARWLPQMFYFFFAYKALKSQNK
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PLVFSCPSGNFGNICAGIMAKKLGLPIEHFVASTNVNDTVPRFLENGKYDPKPSKATISN
CEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHH
AMDVGNPSNFIRIQELYNNDLKAFEKDFSSYSYTDEETLEALKKIYNADGYIAEPHGAVG
HHCCCCCCCCEEHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCCCEE
YLGLKKELEKHPNAIGVFLETAHPIKFLDVVEPALGITLPIPAQIESVINEEKVSVKIRT
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEECCCHHHHHHHHCCHHEEEEEEE
YEELKSFLG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]