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The map label for this gene is glnII [H]

Identifier: 146300099

GI number: 146300099

Start: 2814885

End: 2815901

Strand: Reverse

Name: glnII [H]

Synonym: Fjoh_2344

Alternate gene names: 146300099

Gene position: 2815901-2814885 (Counterclockwise)

Preceding gene: 146300111

Following gene: 146300098

Centisome position: 46.19

GC content: 39.23

Gene sequence:

>1017_bases
ATGGCTAAAATTAAGTTAGAGTACATTTGGTTAGACGGATATGAACCAACTCAAAATCTTAGAAGTAAAACTAAAGTTGA
AGAGCACGAAAATTTCAAAGGAACATTAGAAGAACTTGGAAATTGGTCATTTGACGGTTCGTCAACAAAACAAGCTGAAG
GTGGATCTTCTGACTGTTTATTAGTTCCAGTTGCAATCTATCCAGATCCAACTCGTATTAACGGATGGTTAGTAATGTCT
GAAGTTATGTATGCTGATGGAACTCCGCACCCATCTAACGGAAGAGCCACAATTGATGATGATAATGATGATTTTTGGTT
TGGTTTCGAACAAGAGTATTTCATCATGGATACTAAAACACAACTTCCACTTGGTTTCCCAGTTGGAGGATACCCTGCTC
CACAAGGGATGTACTACTGTTCAGTAGGTGGAAAAAACACTCACGGTAGAAAATTAGTTGAAGAACACGCTGATTTATGT
ATCGCTGCAGGAATCAACTTTGAAGGAATCAACCAAGAGGTTGCCTGCGGACAATGGGAATTCCAATTATTCGCTAAAGG
TGCTAAAAAAGCAGGAGACGAAATCTGGGTTGCTCGTTACTTATTAGACCGTTTAACTGAGAAATATGGTTACTATATTG
AATATCACCCAAAACCTCTAGGAGATACAGACTGGAATGGTTCTGGAATGCATGCTAACTTCTCTAACGAAGTTTTAAGA
ACATGTGGAGACCAGGCTACTTACGAAAGAATTTGCGAAGCTTTCCGTCCTGTTACTGCTGAGCACATCGCAGTTTACGG
AGCTTACAATGACCAACGTTTAACTGGTAAACACGAAACAGCTTCTATTCACGATTTCTCTTATGGAGTTTCAGACAGAG
GATGTTCTATCAGAATTCCTTTAATGACTGTTCAAAAAGGATGGAAAGGATGGCTGGAAGACAGAAGACCAGCTTCAAAC
GGAGACCCTTACAAAATTGCTGCTAGAATTATCAAAACTGTTAAATCAGCTCTATAA

Upstream 100 bases:

>100_bases
AGTACTTCATAAAACAAAATAGCCCCCTAATTTTTAGGACTAAAAAAATAAATCTATATTTGACCCAGCGAAAAAACAAA
AAAAATAAATTTATATTATT

Downstream 100 bases:

>100_bases
TTCAAAACTTACAATATATTATAAAAATGCCGGCATTCTTGCTGGCATTTTTTTATGCATTAAACTCTGCAAATTTTAAA
AACATTTTGAATTTTACACA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase II; Glutamine synthetase II; GSII [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAKIKLEYIWLDGYEPTQNLRSKTKVEEHENFKGTLEELGNWSFDGSSTKQAEGGSSDCLLVPVAIYPDPTRINGWLVMS
EVMYADGTPHPSNGRATIDDDNDDFWFGFEQEYFIMDTKTQLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLC
IAAGINFEGINQEVACGQWEFQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKPLGDTDWNGSGMHANFSNEVLR
TCGDQATYERICEAFRPVTAEHIAVYGAYNDQRLTGKHETASIHDFSYGVSDRGCSIRIPLMTVQKGWKGWLEDRRPASN
GDPYKIAARIIKTVKSAL

Sequences:

>Translated_338_residues
MAKIKLEYIWLDGYEPTQNLRSKTKVEEHENFKGTLEELGNWSFDGSSTKQAEGGSSDCLLVPVAIYPDPTRINGWLVMS
EVMYADGTPHPSNGRATIDDDNDDFWFGFEQEYFIMDTKTQLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLC
IAAGINFEGINQEVACGQWEFQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKPLGDTDWNGSGMHANFSNEVLR
TCGDQATYERICEAFRPVTAEHIAVYGAYNDQRLTGKHETASIHDFSYGVSDRGCSIRIPLMTVQKGWKGWLEDRRPASN
GDPYKIAARIIKTVKSAL
>Mature_337_residues
AKIKLEYIWLDGYEPTQNLRSKTKVEEHENFKGTLEELGNWSFDGSSTKQAEGGSSDCLLVPVAIYPDPTRINGWLVMSE
VMYADGTPHPSNGRATIDDDNDDFWFGFEQEYFIMDTKTQLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLCI
AAGINFEGINQEVACGQWEFQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKPLGDTDWNGSGMHANFSNEVLRT
CGDQATYERICEAFRPVTAEHIAVYGAYNDQRLTGKHETASIHDFSYGVSDRGCSIRIPLMTVQKGWKGWLEDRRPASNG
DPYKIAARIIKTVKSAL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI74271826, Length=342, Percent_Identity=41.812865497076, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI74271837, Length=342, Percent_Identity=41.812865497076, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI19923206, Length=342, Percent_Identity=41.812865497076, Blast_Score=251, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI115532980, Length=337, Percent_Identity=44.5103857566766, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI115532978, Length=337, Percent_Identity=44.5103857566766, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17554028, Length=343, Percent_Identity=42.8571428571429, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17540008, Length=346, Percent_Identity=43.6416184971098, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17538856, Length=345, Percent_Identity=41.1594202898551, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17551038, Length=311, Percent_Identity=39.2282958199357, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6325292, Length=342, Percent_Identity=45.0292397660819, Blast_Score=280, Evalue=2e-76,
Organism=Drosophila melanogaster, GI281360453, Length=342, Percent_Identity=42.6900584795322, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI17136208, Length=342, Percent_Identity=42.6900584795322, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24580558, Length=342, Percent_Identity=42.6900584795322, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24641292, Length=341, Percent_Identity=42.5219941348974, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24641290, Length=341, Percent_Identity=42.5219941348974, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI45554830, Length=265, Percent_Identity=42.6415094339623, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 37990; Mature: 37859

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKLEYIWLDGYEPTQNLRSKTKVEEHENFKGTLEELGNWSFDGSSTKQAEGGSSDCL
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LVPVAIYPDPTRINGWLVMSEVMYADGTPHPSNGRATIDDDNDDFWFGFEQEYFIMDTKT
EEEEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCEEEECCCEEEEEECCC
QLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLCIAAGINFEGINQEVACGQWE
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEECCCEE
FQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKPLGDTDWNGSGMHANFSNEVLR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
TCGDQATYERICEAFRPVTAEHIAVYGAYNDQRLTGKHETASIHDFSYGVSDRGCSIRIP
HCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCEEEEEECCCCCCCCEEEEE
LMTVQKGWKGWLEDRRPASNGDPYKIAARIIKTVKSAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIKLEYIWLDGYEPTQNLRSKTKVEEHENFKGTLEELGNWSFDGSSTKQAEGGSSDCL
CEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LVPVAIYPDPTRINGWLVMSEVMYADGTPHPSNGRATIDDDNDDFWFGFEQEYFIMDTKT
EEEEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCEEEECCCEEEEEECCC
QLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLCIAAGINFEGINQEVACGQWE
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEECCCEE
FQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKPLGDTDWNGSGMHANFSNEVLR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
TCGDQATYERICEAFRPVTAEHIAVYGAYNDQRLTGKHETASIHDFSYGVSDRGCSIRIP
HCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCEEEEEECCCCCCCCEEEEE
LMTVQKGWKGWLEDRRPASNGDPYKIAARIIKTVKSAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]