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The map label for this gene is ascU [H]

Identifier: 145301466

GI number: 145301466

Start: 78991

End: 80049

Strand: Direct

Name: ascU [H]

Synonym: ASA_P5G083

Alternate gene names: 145301466

Gene position: 78991-80049 (Clockwise)

Preceding gene: 145301465

Following gene: 145301467

Centisome position: 50.93

GC content: 59.58

Gene sequence:

>1059_bases
ATGAGCGGCGAGAAAACCGAACAACCCACCCCCAAAAAGTTGCGGGATGCCCGCCAGAAGGGGCAGGTGGTCAAGAGCAA
GGAGGTGGTATCCACCGCCATCATCCTCAGTCTGGTGGGCATGCTGATGGCGATGTCCGACTACTACCTCGAACATCTGG
GCAAGCTGATGATGATCCCGGAATCCCACATCAACCTCCCCTTTACCCAGGCACTCAACCATGTGGTGGAGAACCTGATG
CAGGAGATGGCCTATCTCTGCATTCCGCTCCTCGCGGTCGCTGTGTTGGTGGTGCTAGCCTCCCACTTCGCCCAGTACGG
TTTTCTGCTGAGCGGCGAGTCCATCAAGCCCGACATAAAGAAGATCAATCCGGTGGAGGGGGCCAAGAAGATCTTCTCCA
TCAAGAGCCTGATGGAGTTCGTCAAATCCCTGCTCAAGGTGAGTCTCCTGAGCCTCTTGGTATGGGTCACCATAAAGGGC
AATCTGCAGGCGATGCTCCAGTTGCCCGGCTGCGGCATGAACTGCATCACCCCGGTGCTGGGGAGCATGCTGGGCCAGCT
GATGGCGGTGTGCGCCATGGGCTTTGTGGTGATCGCCATCGCTGACTACGCCTTCGAACACTACCAGCACATCAAGCAGC
TCAAGATGAGCAAGGACGAGGTGAAGCGGGAGTACAAGGAGATGGAGGGAAGCCCGGAGATCAAGAGCAAGCGCCGCCAG
TTCCATCAGGAGTTGCAGTCGAGCAACATGCGTGCCGACGTCAAGCGCTCCTCGGTCATCGTCGCCAACCCGACCCATAT
CGCCATCGGTATCCGCTACGAGCGGGGAGAAACCCCGCTGCCGGTGGTGACCCTCAAATTCACCGATGCCCAGGCGCTGC
AGGTACGCAAGATTGCCGAAGATGAGGGGATCCCGGTGCTGCAACGCATCCCGCTGGCCCGTGCCCTCTACCGGGATGCC
CAACTCAACAACTACATTCCCGCCGACCAGATCGAGGCGACTGCCGAGGTTCTGCGCTGGCTGGAGCAGTGGCGCCAGAG
CGAAGGGCCGCAGTGGTAG

Upstream 100 bases:

>100_bases
TGCTGGTCATCTACATCAAGCTGATGATGGATCACGCCTATCAACAGGTGCTGGCGGTGATGGATCCGCTGCAACTGCTG
ATCCCCATTCTGGAGGCGCG

Downstream 100 bases:

>100_bases
GGCTGCACGCTGCCCGCCTGGCCAAACAAACAGCACAACACGCGAAAAACTGACAAATCCCCGACCGCTCCCTGATATAG
CGCGCCGTCACTGCTCGGTA

Product: AscU protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSGEKTEQPTPKKLRDARQKGQVVKSKEVVSTAIILSLVGMLMAMSDYYLEHLGKLMMIPESHINLPFTQALNHVVENLM
QEMAYLCIPLLAVAVLVVLASHFAQYGFLLSGESIKPDIKKINPVEGAKKIFSIKSLMEFVKSLLKVSLLSLLVWVTIKG
NLQAMLQLPGCGMNCITPVLGSMLGQLMAVCAMGFVVIAIADYAFEHYQHIKQLKMSKDEVKREYKEMEGSPEIKSKRRQ
FHQELQSSNMRADVKRSSVIVANPTHIAIGIRYERGETPLPVVTLKFTDAQALQVRKIAEDEGIPVLQRIPLARALYRDA
QLNNYIPADQIEATAEVLRWLEQWRQSEGPQW

Sequences:

>Translated_352_residues
MSGEKTEQPTPKKLRDARQKGQVVKSKEVVSTAIILSLVGMLMAMSDYYLEHLGKLMMIPESHINLPFTQALNHVVENLM
QEMAYLCIPLLAVAVLVVLASHFAQYGFLLSGESIKPDIKKINPVEGAKKIFSIKSLMEFVKSLLKVSLLSLLVWVTIKG
NLQAMLQLPGCGMNCITPVLGSMLGQLMAVCAMGFVVIAIADYAFEHYQHIKQLKMSKDEVKREYKEMEGSPEIKSKRRQ
FHQELQSSNMRADVKRSSVIVANPTHIAIGIRYERGETPLPVVTLKFTDAQALQVRKIAEDEGIPVLQRIPLARALYRDA
QLNNYIPADQIEATAEVLRWLEQWRQSEGPQW
>Mature_351_residues
SGEKTEQPTPKKLRDARQKGQVVKSKEVVSTAIILSLVGMLMAMSDYYLEHLGKLMMIPESHINLPFTQALNHVVENLMQ
EMAYLCIPLLAVAVLVVLASHFAQYGFLLSGESIKPDIKKINPVEGAKKIFSIKSLMEFVKSLLKVSLLSLLVWVTIKGN
LQAMLQLPGCGMNCITPVLGSMLGQLMAVCAMGFVVIAIADYAFEHYQHIKQLKMSKDEVKREYKEMEGSPEIKSKRRQF
HQELQSSNMRADVKRSSVIVANPTHIAIGIRYERGETPLPVVTLKFTDAQALQVRKIAEDEGIPVLQRIPLARALYRDAQ
LNNYIPADQIEATAEVLRWLEQWRQSEGPQW

Specific function: Component of the yop secretion machinery [H]

COG id: COG4792

COG function: function code U; Type III secretory pathway, component EscU

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=359, Percent_Identity=32.033426183844, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 39600; Mature: 39469

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEKTEQPTPKKLRDARQKGQVVKSKEVVSTAIILSLVGMLMAMSDYYLEHLGKLMMIP
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ESHINLPFTQALNHVVENLMQEMAYLCIPLLAVAVLVVLASHFAQYGFLLSGESIKPDIK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHH
KINPVEGAKKIFSIKSLMEFVKSLLKVSLLSLLVWVTIKGNLQAMLQLPGCGMNCITPVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHH
GSMLGQLMAVCAMGFVVIAIADYAFEHYQHIKQLKMSKDEVKREYKEMEGSPEIKSKRRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
FHQELQSSNMRADVKRSSVIVANPTHIAIGIRYERGETPLPVVTLKFTDAQALQVRKIAE
HHHHHHHCCCCHHHHHCEEEEECCCEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHC
DEGIPVLQRIPLARALYRDAQLNNYIPADQIEATAEVLRWLEQWRQSEGPQW
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGEKTEQPTPKKLRDARQKGQVVKSKEVVSTAIILSLVGMLMAMSDYYLEHLGKLMMIP
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
ESHINLPFTQALNHVVENLMQEMAYLCIPLLAVAVLVVLASHFAQYGFLLSGESIKPDIK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHH
KINPVEGAKKIFSIKSLMEFVKSLLKVSLLSLLVWVTIKGNLQAMLQLPGCGMNCITPVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHH
GSMLGQLMAVCAMGFVVIAIADYAFEHYQHIKQLKMSKDEVKREYKEMEGSPEIKSKRRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
FHQELQSSNMRADVKRSSVIVANPTHIAIGIRYERGETPLPVVTLKFTDAQALQVRKIAE
HHHHHHHCCCCHHHHHCEEEEECCCEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHC
DEGIPVLQRIPLARALYRDAQLNNYIPADQIEATAEVLRWLEQWRQSEGPQW
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9746557; 9748454; 11586360 [H]