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The map label for this gene is aopH [H]

Identifier: 145301394

GI number: 145301394

Start: 7609

End: 9000

Strand: Direct

Name: aopH [H]

Synonym: ASA_P5G009

Alternate gene names: 145301394

Gene position: 7609-9000 (Clockwise)

Preceding gene: 145301390

Following gene: 145301396

Centisome position: 4.91

GC content: 59.05

Gene sequence:

>1392_bases
ATGAACCTGACTATTCAAACCCTTCACCAACAGGTATCCCGACTGGCCGAACAGCAGAACGGCGAGCTGATAGGCAAATT
GAGAGGCAATGTTGCCGCCAATCGCGAGGGGAGTTTTCAGGGCCTGACGGTGACCAATGGTGCTCGAGACACCGAAAAGG
CATTTGCTCAGGAGGTTCTCCGCCACGTGCAGGATATTGCCCTCAATCAGGACGATGTCGCTGCGCTGCACAAAAGCGCT
ATCCGCTTCAATCACAATAACTTCGAACTGCGCAGCATCGGCAATAGCCAGAGTCAGTTGGTGGGATTGCGCAGCGATCA
GCTGACCCTCAAGGACGCCAAGACCTTGCTGGATGCCGCCATGCGTCAGCAGGGAGCACAGCATCACGCAGCCAGCGCGC
CAGCCGGTCAGGATGCGCCGCCATTGCCGCCACGTCGTGCTCCGGCCCTTCCTCCGCACAATGCCGGCCCGGCACTGGCC
CAGCAGCATGAACGACTCAGGAGTGCCGCGCCCAGAATGGTGACCCCTGCGGCGGTTGAGCCCTATGGGGCCCAGACGAA
GCGGGAACTGAGCAGCAGGCTGGCCGGTTTGCAGCAACAGTTGCAACCCTCGGTGCAACCAGATCGTTATCTGCAAAGCC
CTGATGGTAGCAAGCTCAACCGCTTCAGAGATATTCAGGCCAACAAGGCGACCGCAGTACGCGCAGATCTCAATGCCAAC
TATGTACAGGCTGGCGCCCATAGATCCATCGCCTGCCAGTACCCGCTTGAGAGTCAGTTGGAGAGCCATCTGCAGATGCT
GTTCGACAACCGGACACCGGTTCTGGCAGTCCTGGCTTCCGCCCACGAGATCGCCAACCCCCAGTTCAATATGCCCAACT
ATTTCCGCCAAAACGGGCAGTATGGGGCTATGTCGGTCACATCCACGCATAAAGAGAGCATCGACCTTGGTGATGGTATT
CAGGCCGATGTCTATCAGATGGCGCTGACCATGCCGGGTTCGGGCAAGAAGGGGATCGCAGTACCTGTCGTCCATGTCAG
CAACTGGCCGGATAAAACGGCTGTGAGTGCCGAGGTGACCGAGCAGCTCTCTGCCCTGCTGGAGCAAAAGGCGCAAGAGA
AGCGTGCCATCTATGAAAAGGCGGGCAGTTCGGCGGTAGGTGATGCCAACAAGCTGCTGCCAGTGGTTCACTGCCGTGCC
GGGGTGGGTCGTACCGCACAGGTGATTGGTTCCATGGCGATGCACGATCCGCGCAATGCCGCCCTGAGCATGGAGGAGGT
GGTTAGCGACATGCGTACCAGCCGCAACGGAGTCATGGTGCAAAAACAGGAGCAGCTGGACGTGCTGATGACGCTGGCCG
ATCGCCAGCAACGTCCTCTGCTGCGGGCATAA

Upstream 100 bases:

>100_bases
GCCATTTTGCACCTTGCCTAAAAATATCCGGCTGGCAGTGCAAGGATGAAGAAAAGCCGCTTTCTATACTCGCGACTCAC
TCATGCCATCCGAGGTAAAG

Downstream 100 bases:

>100_bases
CCGTCGATATGCCCCTGGCGGGTTGATTCGATAAACGCAAAGAGGCCACCCCGTGGGGTTAATGCCAGTCAGTTAAGCCT
GACTGGCATTGTTTTTATTA

Product: putative protein-tyrosine phosphatase Aop effector AopH

Products: NA

Alternate protein names: Virulence protein [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNLTIQTLHQQVSRLAEQQNGELIGKLRGNVAANREGSFQGLTVTNGARDTEKAFAQEVLRHVQDIALNQDDVAALHKSA
IRFNHNNFELRSIGNSQSQLVGLRSDQLTLKDAKTLLDAAMRQQGAQHHAASAPAGQDAPPLPPRRAPALPPHNAGPALA
QQHERLRSAAPRMVTPAAVEPYGAQTKRELSSRLAGLQQQLQPSVQPDRYLQSPDGSKLNRFRDIQANKATAVRADLNAN
YVQAGAHRSIACQYPLESQLESHLQMLFDNRTPVLAVLASAHEIANPQFNMPNYFRQNGQYGAMSVTSTHKESIDLGDGI
QADVYQMALTMPGSGKKGIAVPVVHVSNWPDKTAVSAEVTEQLSALLEQKAQEKRAIYEKAGSSAVGDANKLLPVVHCRA
GVGRTAQVIGSMAMHDPRNAALSMEEVVSDMRTSRNGVMVQKQEQLDVLMTLADRQQRPLLRA

Sequences:

>Translated_463_residues
MNLTIQTLHQQVSRLAEQQNGELIGKLRGNVAANREGSFQGLTVTNGARDTEKAFAQEVLRHVQDIALNQDDVAALHKSA
IRFNHNNFELRSIGNSQSQLVGLRSDQLTLKDAKTLLDAAMRQQGAQHHAASAPAGQDAPPLPPRRAPALPPHNAGPALA
QQHERLRSAAPRMVTPAAVEPYGAQTKRELSSRLAGLQQQLQPSVQPDRYLQSPDGSKLNRFRDIQANKATAVRADLNAN
YVQAGAHRSIACQYPLESQLESHLQMLFDNRTPVLAVLASAHEIANPQFNMPNYFRQNGQYGAMSVTSTHKESIDLGDGI
QADVYQMALTMPGSGKKGIAVPVVHVSNWPDKTAVSAEVTEQLSALLEQKAQEKRAIYEKAGSSAVGDANKLLPVVHCRA
GVGRTAQVIGSMAMHDPRNAALSMEEVVSDMRTSRNGVMVQKQEQLDVLMTLADRQQRPLLRA
>Mature_463_residues
MNLTIQTLHQQVSRLAEQQNGELIGKLRGNVAANREGSFQGLTVTNGARDTEKAFAQEVLRHVQDIALNQDDVAALHKSA
IRFNHNNFELRSIGNSQSQLVGLRSDQLTLKDAKTLLDAAMRQQGAQHHAASAPAGQDAPPLPPRRAPALPPHNAGPALA
QQHERLRSAAPRMVTPAAVEPYGAQTKRELSSRLAGLQQQLQPSVQPDRYLQSPDGSKLNRFRDIQANKATAVRADLNAN
YVQAGAHRSIACQYPLESQLESHLQMLFDNRTPVLAVLASAHEIANPQFNMPNYFRQNGQYGAMSVTSTHKESIDLGDGI
QADVYQMALTMPGSGKKGIAVPVVHVSNWPDKTAVSAEVTEQLSALLEQKAQEKRAIYEKAGSSAVGDANKLLPVVHCRA
GVGRTAQVIGSMAMHDPRNAALSMEEVVSDMRTSRNGVMVQKQEQLDVLMTLADRQQRPLLRA

Specific function: Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of yopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via type III secretion system [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tyrosine-protein phosphatase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000387
- InterPro:   IPR015103
- InterPro:   IPR016130
- InterPro:   IPR003546
- InterPro:   IPR000242 [H]

Pfam domain/function: PF00102 Y_phosphatase; PF09013 YopH_N [H]

EC number: =3.1.3.48 [H]

Molecular weight: Translated: 50415; Mature: 50415

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00383 TYR_PHOSPHATASE_1 ; PS50056 TYR_PHOSPHATASE_2 ; PS50055 TYR_PHOSPHATASE_PTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTIQTLHQQVSRLAEQQNGELIGKLRGNVAANREGSFQGLTVTNGARDTEKAFAQEVL
CCCHHHHHHHHHHHHHHHCCCCEEEHHHCCEECCCCCCCCCEEEECCCCHHHHHHHHHHH
RHVQDIALNQDDVAALHKSAIRFNHNNFELRSIGNSQSQLVGLRSDQLTLKDAKTLLDAA
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHEEECCCCCCHHHHHHHHHHHH
MRQQGAQHHAASAPAGQDAPPLPPRRAPALPPHNAGPALAQQHERLRSAAPRMVTPAAVE
HHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PYGAQTKRELSSRLAGLQQQLQPSVQPDRYLQSPDGSKLNRFRDIQANKATAVRADLNAN
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
YVQAGAHRSIACQYPLESQLESHLQMLFDNRTPVLAVLASAHEIANPQFNMPNYFRQNGQ
HHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCC
YGAMSVTSTHKESIDLGDGIQADVYQMALTMPGSGKKGIAVPVVHVSNWPDKTAVSAEVT
CCEEEECCCCHHCCCCCCCCHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCHHHHHHHH
EQLSALLEQKAQEKRAIYEKAGSSAVGDANKLLPVVHCRAGVGRTAQVIGSMAMHDPRNA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
ALSMEEVVSDMRTSRNGVMVQKQEQLDVLMTLADRQQRPLLRA
HCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLTIQTLHQQVSRLAEQQNGELIGKLRGNVAANREGSFQGLTVTNGARDTEKAFAQEVL
CCCHHHHHHHHHHHHHHHCCCCEEEHHHCCEECCCCCCCCCEEEECCCCHHHHHHHHHHH
RHVQDIALNQDDVAALHKSAIRFNHNNFELRSIGNSQSQLVGLRSDQLTLKDAKTLLDAA
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHEEECCCCCCHHHHHHHHHHHH
MRQQGAQHHAASAPAGQDAPPLPPRRAPALPPHNAGPALAQQHERLRSAAPRMVTPAAVE
HHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PYGAQTKRELSSRLAGLQQQLQPSVQPDRYLQSPDGSKLNRFRDIQANKATAVRADLNAN
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
YVQAGAHRSIACQYPLESQLESHLQMLFDNRTPVLAVLASAHEIANPQFNMPNYFRQNGQ
HHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCC
YGAMSVTSTHKESIDLGDGIQADVYQMALTMPGSGKKGIAVPVVHVSNWPDKTAVSAEVT
CCEEEECCCCHHCCCCCCCCHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCHHHHHHHH
EQLSALLEQKAQEKRAIYEKAGSSAVGDANKLLPVVHCRAGVGRTAQVIGSMAMHDPRNA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
ALSMEEVVSDMRTSRNGVMVQKQEQLDVLMTLADRQQRPLLRA
HCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3244311; 2166336; 1429715; 8052312; 8702535 [H]