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The map label for this gene is yneF [H]

Identifier: 145300080

GI number: 145300080

Start: 3442638

End: 3444257

Strand: Direct

Name: yneF [H]

Synonym: ASA_3180

Alternate gene names: 145300080

Gene position: 3442638-3444257 (Clockwise)

Preceding gene: 145300079

Following gene: 145300081

Centisome position: 73.21

GC content: 59.2

Gene sequence:

>1620_bases
ATGAATGATATCGCTGCCGTAGCAGGACAATTGGCCAGACTCAGGGAATCCCTTCAGCAAAAGCAGCTGGAGCTAGACGG
TATAGTCACTTTTTCCGATGCAGAGCGAGCCCGCAGTATCCAGTTCATCAGTAAATTGATGCAAGCCTGTCGTGGCCATG
ACAGGGAGCTGGACAACAAGCTCGCCAAGCTCAAGCAAAAACTCGACGGCAATCCCCCGCTCTCCTCGCTGGATGCCGAC
CTCGCCGTCATCGAAAAGCTGTTGCAGCAACATGGCAATGCGCTTGAAGAGTCCATCAGCCTGACCAAGGCTGCCATCGA
CCTTGGCACCAAACAGCTCAAATCCATCAAGGGCTTCCCGGAGGATACCCGCAAGTCCCTCAAGGAGTTTCTGGCAAGCC
CGGATGGCTATGGCATCGGCGACCATCAAAAGAAAGTGATCCGCCTGCTGGAGTTTTACCAGCAGGCCATCAAGATCCAG
CTGCTGCCGACCCAGCCCCTGCTAACCCCGGCTCCCGCCGCCAGCGTGCAGGCCATGGCCATCACAGAGCTCTCGTCCCC
TGAGCCTGTGATCCCGTTTGACGCCAAGGCTCACGCCCATATCGCCGACGAGTTGCAACGGCTCATCACAGAGCTGGATT
TTGCCGGCGCCGTTGGCGAGAGTCTGGCGGATATCCGCCGCCAGTTGCTCAATGGCATCACGCCGCCGCTGCTGGCGGAG
ATCTGCCTGCAGGTGATAGAGCTCATCATTGAGGGCACCCGCGAGGAGCGAAAATCCTCCCAGGCTTTCCTGAGCACCCT
CAACGAGAGCCTCTCTGCGGTGCATTTCAGCTTCACCGAATCCCTCGACGAAGGGCGCGCCCTGCAAAGCGCCACTCAGG
ATACCCGTCAGGCCATCGACAAGGAGCTACGGGCCCTCGACCATACCTTGGCCAGCCACGACACCTTCGACGAGCTCAAG
GGTGCCATCTCGGGCCATATGGGTGCCATCAACATGCTTTTGCAGGACAGGGAGGCCATGGCGGCCCGTGAGCGCCACCT
GCTCACCCGCCTCTCCACCATGGAGGCCAAGCTGCGGCTGATGAAGGAGGAGACCGCCGAGTACAAGAAGCGGCTCACCA
TCCAGAAACACAAATTGTTCCTCGACAGCCTGACCCAGGTGCATAACCGGGCGGCGCTGGACGAGCGGCTCGAACTCGAA
TACAAGCGCTGGCTGCGCTACAACACTCCGCTCTGTCTGGCCATCATAGACATTGATCACTTCAAGAACATCAATGACAA
CTATGGCCACATGGCCGGTGACAAGGCGTTGAAGGTCGTGGCCAAGGCATTGCAGGGCGCCCTGCGCGATACCGACTTCA
TCGCCCGCTTTGGCGGTGAGGAGTTCGTGGTGCTGCTGCCGAACATCAACCCGGACAAATACCAGAAGCCGCTGGAGACA
CTGCGCCAGACCATCAAGAGCATTCCGTTCCGCTTTCGGGATGCCAGGGTCGAGATCACCATCTCCATCGGTGCCACCCT
GTTTCGGGAAGGGGATCACACCACGGATGCGTTCGAGCGAGCCGACAAGGCGCTCTACAGCTCCAAGCACTCGGGTCGGG
ATCAGGTCAATCTGGCCTGA

Upstream 100 bases:

>100_bases
AACCAATAACCCTATAGATAATCAACTGGATCTGGAACTGGGTTCGACCGCCGTTGCTATCCAACACAAAAACGATAATG
ACAACAGGGCGACCTGAATA

Downstream 100 bases:

>100_bases
ATCCAGGCTGAGTGGCGGCTAAAAAAGCAGCAAACGCACTTCTCCCCTATTCCCCTGCCCTACCGCTTACATCTACTATC
AAATTCAAGGGATGCCACAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNDIAAVAGQLARLRESLQQKQLELDGIVTFSDAERARSIQFISKLMQACRGHDRELDNKLAKLKQKLDGNPPLSSLDAD
LAVIEKLLQQHGNALEESISLTKAAIDLGTKQLKSIKGFPEDTRKSLKEFLASPDGYGIGDHQKKVIRLLEFYQQAIKIQ
LLPTQPLLTPAPAASVQAMAITELSSPEPVIPFDAKAHAHIADELQRLITELDFAGAVGESLADIRRQLLNGITPPLLAE
ICLQVIELIIEGTREERKSSQAFLSTLNESLSAVHFSFTESLDEGRALQSATQDTRQAIDKELRALDHTLASHDTFDELK
GAISGHMGAINMLLQDREAMAARERHLLTRLSTMEAKLRLMKEETAEYKKRLTIQKHKLFLDSLTQVHNRAALDERLELE
YKRWLRYNTPLCLAIIDIDHFKNINDNYGHMAGDKALKVVAKALQGALRDTDFIARFGGEEFVVLLPNINPDKYQKPLET
LRQTIKSIPFRFRDARVEITISIGATLFREGDHTTDAFERADKALYSSKHSGRDQVNLA

Sequences:

>Translated_539_residues
MNDIAAVAGQLARLRESLQQKQLELDGIVTFSDAERARSIQFISKLMQACRGHDRELDNKLAKLKQKLDGNPPLSSLDAD
LAVIEKLLQQHGNALEESISLTKAAIDLGTKQLKSIKGFPEDTRKSLKEFLASPDGYGIGDHQKKVIRLLEFYQQAIKIQ
LLPTQPLLTPAPAASVQAMAITELSSPEPVIPFDAKAHAHIADELQRLITELDFAGAVGESLADIRRQLLNGITPPLLAE
ICLQVIELIIEGTREERKSSQAFLSTLNESLSAVHFSFTESLDEGRALQSATQDTRQAIDKELRALDHTLASHDTFDELK
GAISGHMGAINMLLQDREAMAARERHLLTRLSTMEAKLRLMKEETAEYKKRLTIQKHKLFLDSLTQVHNRAALDERLELE
YKRWLRYNTPLCLAIIDIDHFKNINDNYGHMAGDKALKVVAKALQGALRDTDFIARFGGEEFVVLLPNINPDKYQKPLET
LRQTIKSIPFRFRDARVEITISIGATLFREGDHTTDAFERADKALYSSKHSGRDQVNLA
>Mature_539_residues
MNDIAAVAGQLARLRESLQQKQLELDGIVTFSDAERARSIQFISKLMQACRGHDRELDNKLAKLKQKLDGNPPLSSLDAD
LAVIEKLLQQHGNALEESISLTKAAIDLGTKQLKSIKGFPEDTRKSLKEFLASPDGYGIGDHQKKVIRLLEFYQQAIKIQ
LLPTQPLLTPAPAASVQAMAITELSSPEPVIPFDAKAHAHIADELQRLITELDFAGAVGESLADIRRQLLNGITPPLLAE
ICLQVIELIIEGTREERKSSQAFLSTLNESLSAVHFSFTESLDEGRALQSATQDTRQAIDKELRALDHTLASHDTFDELK
GAISGHMGAINMLLQDREAMAARERHLLTRLSTMEAKLRLMKEETAEYKKRLTIQKHKLFLDSLTQVHNRAALDERLELE
YKRWLRYNTPLCLAIIDIDHFKNINDNYGHMAGDKALKVVAKALQGALRDTDFIARFGGEEFVVLLPNINPDKYQKPLET
LRQTIKSIPFRFRDARVEITISIGATLFREGDHTTDAFERADKALYSSKHSGRDQVNLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=38.8888888888889, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693134, Length=227, Percent_Identity=28.6343612334802, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787262, Length=218, Percent_Identity=29.8165137614679, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081881, Length=197, Percent_Identity=34.5177664974619, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=212, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082007, Length=256, Percent_Identity=28.90625, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=30.5389221556886, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=33.5443037974684, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=150, Percent_Identity=29.3333333333333, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=34.3195266272189, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=32.5153374233129, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=33.974358974359, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=29.7142857142857, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 60346; Mature: 60346

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIAAVAGQLARLRESLQQKQLELDGIVTFSDAERARSIQFISKLMQACRGHDRELDNK
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAKLKQKLDGNPPLSSLDADLAVIEKLLQQHGNALEESISLTKAAIDLGTKQLKSIKGFP
HHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDTRKSLKEFLASPDGYGIGDHQKKVIRLLEFYQQAIKIQLLPTQPLLTPAPAASVQAMA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHH
ITELSSPEPVIPFDAKAHAHIADELQRLITELDFAGAVGESLADIRRQLLNGITPPLLAE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ICLQVIELIIEGTREERKSSQAFLSTLNESLSAVHFSFTESLDEGRALQSATQDTRQAID
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELRALDHTLASHDTFDELKGAISGHMGAINMLLQDREAMAARERHLLTRLSTMEAKLRL
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKEETAEYKKRLTIQKHKLFLDSLTQVHNRAALDERLELEYKRWLRYNTPLCLAIIDIDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHH
FKNINDNYGHMAGDKALKVVAKALQGALRDTDFIARFGGEEFVVLLPNINPDKYQKPLET
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
LRQTIKSIPFRFRDARVEITISIGATLFREGDHTTDAFERADKALYSSKHSGRDQVNLA
HHHHHHHCCCEEECCEEEEEEEECHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNDIAAVAGQLARLRESLQQKQLELDGIVTFSDAERARSIQFISKLMQACRGHDRELDNK
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAKLKQKLDGNPPLSSLDADLAVIEKLLQQHGNALEESISLTKAAIDLGTKQLKSIKGFP
HHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
EDTRKSLKEFLASPDGYGIGDHQKKVIRLLEFYQQAIKIQLLPTQPLLTPAPAASVQAMA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHH
ITELSSPEPVIPFDAKAHAHIADELQRLITELDFAGAVGESLADIRRQLLNGITPPLLAE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ICLQVIELIIEGTREERKSSQAFLSTLNESLSAVHFSFTESLDEGRALQSATQDTRQAID
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELRALDHTLASHDTFDELKGAISGHMGAINMLLQDREAMAARERHLLTRLSTMEAKLRL
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKEETAEYKKRLTIQKHKLFLDSLTQVHNRAALDERLELEYKRWLRYNTPLCLAIIDIDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHH
FKNINDNYGHMAGDKALKVVAKALQGALRDTDFIARFGGEEFVVLLPNINPDKYQKPLET
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
LRQTIKSIPFRFRDARVEITISIGATLFREGDHTTDAFERADKALYSSKHSGRDQVNLA
HHHHHHHCCCEEECCEEEEEEEECHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]