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The map label for this gene is dosC [H]

Identifier: 145300031

GI number: 145300031

Start: 3383035

End: 3383967

Strand: Direct

Name: dosC [H]

Synonym: ASA_3130

Alternate gene names: 145300031

Gene position: 3383035-3383967 (Clockwise)

Preceding gene: 145300029

Following gene: 145300033

Centisome position: 71.94

GC content: 62.17

Gene sequence:

>933_bases
TTGGGGCATGGCATCGAGTTGTGGGTTCGGCAGATGGAGCGGCTCACGCCGCTGGCAAGTCAGGATCGCAACCAGTTGCT
CGAGCTGTTGCTGGCACAGTCGGATCTCGACAGCCTGCTGGCAACCTTTGCCGATCGTGCGGCAAGAGTTGTCCGTATAC
ATAGCCTCTATTTTGACAACCCCCAGCCACAGAGGCTGATCCAGCAGCCACCCCAGGCCAGCCTGACGCTCCACAGCTAC
CCGTTTGAACTGCGCGGCCAGCACGGTCAGCTGTTCGGCCAGTTGCACTACACCCTGGAACACATACTGAGCGGCAGCCA
GCAGCGGATCCTGCAGCAATACCATCAGCTGCTCTGCCAACCGCTGCCACTCTACCTGCGGCTGGCGCAGCTGGAGCAGC
AAGTACGACTCGATCACCTGACAGGGCTCGGCAACCGCTCCTATTTCGACGAGGCCATAGGTCGCGCCGTGGAGCAACAC
AGCCGGGAATCCCACGGTCTGGTGCTGGTGCTGCTGGATCTCGATCACTTCAAACAGATCAACGACACCTGGGGTCACCC
GGTCGGCGATCTGGTGCTGAGCCGCTTTGCCCATCTGCTCCAGAGCTGCATCCGCAGCACGGATCAGGCCTTTCGCCTCG
GTGGCGACGAGTTTGCCCTCTTGCTGCAACCCGCCGAGCCCGAGGCCTGGCGCCCGGTCTGGCTGCGGCTGCAACACATG
CTGCTGACCCACAAGGAACTCAGCACCTTCTCGGTCGGTTGCAGTCTGGGGGCGGCCAGCTGGCAATCAGGGGTCGCAGT
CCAGAGCCTGTATGAGGCGGCCGATGCTCATCTCTATGCCCGCAAAAAGCCGGTAAAGCGGGTCTGCTGGACTGAGTACC
ACAGCGCCAGGATCGTAAAAAGGCCCGCCAGGCTAGCTGCATGGCGGGCCTGA

Upstream 100 bases:

>100_bases
CTCACATTGAACATATTTTAGGCACAGGGATTGCAGGGAAGTGGCAGGTAGTAGGACGTATGAGGGCTGTACCGTGGAAA
ACACATCGTCATTGAATGGT

Downstream 100 bases:

>100_bases
GCTCACTGCGGGATGCCGCTGTCGGCCGCTGGCAGCCACTGCCATCCCATCCCCGCTTCTTGCGTCATAAATCCGTCACA
GATAAAGAGTCCGGCTACTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGHGIELWVRQMERLTPLASQDRNQLLELLLAQSDLDSLLATFADRAARVVRIHSLYFDNPQPQRLIQQPPQASLTLHSY
PFELRGQHGQLFGQLHYTLEHILSGSQQRILQQYHQLLCQPLPLYLRLAQLEQQVRLDHLTGLGNRSYFDEAIGRAVEQH
SRESHGLVLVLLDLDHFKQINDTWGHPVGDLVLSRFAHLLQSCIRSTDQAFRLGGDEFALLLQPAEPEAWRPVWLRLQHM
LLTHKELSTFSVGCSLGAASWQSGVAVQSLYEAADAHLYARKKPVKRVCWTEYHSARIVKRPARLAAWRA

Sequences:

>Translated_310_residues
MGHGIELWVRQMERLTPLASQDRNQLLELLLAQSDLDSLLATFADRAARVVRIHSLYFDNPQPQRLIQQPPQASLTLHSY
PFELRGQHGQLFGQLHYTLEHILSGSQQRILQQYHQLLCQPLPLYLRLAQLEQQVRLDHLTGLGNRSYFDEAIGRAVEQH
SRESHGLVLVLLDLDHFKQINDTWGHPVGDLVLSRFAHLLQSCIRSTDQAFRLGGDEFALLLQPAEPEAWRPVWLRLQHM
LLTHKELSTFSVGCSLGAASWQSGVAVQSLYEAADAHLYARKKPVKRVCWTEYHSARIVKRPARLAAWRA
>Mature_309_residues
GHGIELWVRQMERLTPLASQDRNQLLELLLAQSDLDSLLATFADRAARVVRIHSLYFDNPQPQRLIQQPPQASLTLHSYP
FELRGQHGQLFGQLHYTLEHILSGSQQRILQQYHQLLCQPLPLYLRLAQLEQQVRLDHLTGLGNRSYFDEAIGRAVEQHS
RESHGLVLVLLDLDHFKQINDTWGHPVGDLVLSRFAHLLQSCIRSTDQAFRLGGDEFALLLQPAEPEAWRPVWLRLQHML
LTHKELSTFSVGCSLGAASWQSGVAVQSLYEAADAHLYARKKPVKRVCWTEYHSARIVKRPARLAAWRA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=154, Percent_Identity=33.1168831168831, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=172, Percent_Identity=31.3953488372093, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787262, Length=152, Percent_Identity=33.5526315789474, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=35.2564102564103, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786584, Length=149, Percent_Identity=32.2147651006711, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=33.5443037974684, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788381, Length=111, Percent_Identity=41.4414414414414, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=33.1360946745562, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=108, Percent_Identity=41.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=29.746835443038, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=32.7272727272727, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787541, Length=149, Percent_Identity=30.8724832214765, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081977, Length=187, Percent_Identity=28.3422459893048, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35484; Mature: 35353

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHGIELWVRQMERLTPLASQDRNQLLELLLAQSDLDSLLATFADRAARVVRIHSLYFDN
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQPQRLIQQPPQASLTLHSYPFELRGQHGQLFGQLHYTLEHILSGSQQRILQQYHQLLCQ
CCHHHHHCCCCCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
PLPLYLRLAQLEQQVRLDHLTGLGNRSYFDEAIGRAVEQHSRESHGLVLVLLDLDHFKQI
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
NDTWGHPVGDLVLSRFAHLLQSCIRSTDQAFRLGGDEFALLLQPAEPEAWRPVWLRLQHM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
LLTHKELSTFSVGCSLGAASWQSGVAVQSLYEAADAHLYARKKPVKRVCWTEYHSARIVK
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RPARLAAWRA
HHHHHHCCCC
>Mature Secondary Structure 
GHGIELWVRQMERLTPLASQDRNQLLELLLAQSDLDSLLATFADRAARVVRIHSLYFDN
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQPQRLIQQPPQASLTLHSYPFELRGQHGQLFGQLHYTLEHILSGSQQRILQQYHQLLCQ
CCHHHHHCCCCCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
PLPLYLRLAQLEQQVRLDHLTGLGNRSYFDEAIGRAVEQHSRESHGLVLVLLDLDHFKQI
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
NDTWGHPVGDLVLSRFAHLLQSCIRSTDQAFRLGGDEFALLLQPAEPEAWRPVWLRLQHM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
LLTHKELSTFSVGCSLGAASWQSGVAVQSLYEAADAHLYARKKPVKRVCWTEYHSARIVK
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RPARLAAWRA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]