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The map label for this gene is malS [H]

Identifier: 145300024

GI number: 145300024

Start: 3374185

End: 3376413

Strand: Direct

Name: malS [H]

Synonym: ASA_3123

Alternate gene names: 145300024

Gene position: 3374185-3376413 (Clockwise)

Preceding gene: 145300023

Following gene: 145300026

Centisome position: 71.75

GC content: 63.12

Gene sequence:

>2229_bases
ATGCGTCGCTCTCCTCGCTTCTCTCCCCTCTGGCTCACCCTGCTGGCCGGCCCCCTGTGTTCAGGCCTGGTGCTGGCCTC
CCCCGAGGTACATCTCTATGTGGATGGCAAACCTGTGGCCGAGGTGCTGACGCTGGACAAGGGCACCGTCAGCCTGACCC
ACAAGCTGGACAAGGGCAGCCACCAGATCCGCATCGGCGATGCCGGCAACAGTTGCGGCACCAGCTTCGGTCCGACCGAG
GCCAAGCCGCTCCCCTTCGGCACCGCCCAACCCATGGACAAATGCGCCAAGGGGACAAGTTTCACCCTGCGGGTGATGCT
GGCCGGGGACTACGAGTTTACCTTCAACCCCGACACCCCCAGCCTCAAGGTGCTGCGGGCGACCAGGAAGAGCGAGTTCA
AGCGCCAGCCCCCGACAGAGCCCTGTATCGCCTGGGATGGCGGCCCTGTGACGGTGTCGCTGAAGGGAGCCTGGCCTGAC
GGCACCCGGCTGCGGGATGCCTACTCAAAACAGGAGGCCGTGGTCACACAGGGCAAGCTGACCCTGACCCCGAGCAAGGA
GAGCGGCGGCCTGCTGCTGCTCGAACCGGTCAAGGCAGCCAAACAAGCACCTTTCAGCTGGGATCAGGCGAGCGTCTATT
TCTTGCTGACCGATCGCTTCCACAACGGCGATCCCGCCAACGACCACAGTTTCGGCCGCCAGAAGGATGGTCAGGACGAG
GTCGCCACCTGGCACGGCGGCGATTTCAAAGGGCTGACCGAGAAGCTCGACTACATCAAGAGCCTCGGCATGAACGCCAT
CTGGATCACTCCCATGGTGGAGCAGGTACACGGCTTCATCGGCGGCGGCGAGCAGGGCAACTTCCCCTTCTACGCCTACC
ACGGCTACTGGGCACTGGATTTCACCAAGATCGATCCCAACTACGGCGACGAGGAGAGCCTGAGAACCCTGGTGGACGAG
GCCCACAAGCGCGGCATGCGGATCATCCTCGATGTGGTGATGAACCACGCCGGTTACGCCACCCTGGCCGATCTGCAGGA
TCTGGGGCTCACCGATCTCACCCAGAACACTGGCAAACTGCCGGCCACCTGGAACCAGTTGCGTCCGAGTGGCGGCCTCA
ACTGGCATGGCTACAACCAGTTTATCGACTACCAATCACCAGACTGGAACAAGTGGTGGAGCGGTGACTGGGTGCGCGCC
GGCCTGCCCGGCTACCCGCAGCCAGGCACAGACGATGTCACCGGTTCGATGGCGGGGCTGCCGGACTTCCTGACCGAGTC
CACCAAACCGGTGGGCTTGCCGCCACTGCTGGCGGCGAAGAAAGACACCAGAGCCAAGGCGCTGCCCAATGCCACTGTGA
GCGACTACCTGATCGCCTGGCACACCGACTGGGTGCGCCGCTTCGGCATCGACGGCTTCCGCGCCGACACCGTCAAGCAT
CTGGAACCCGCCGTCTGGGCCAAGCTCAAGCAGGCGGGCACGGCGGCACTCAAGGAGTGGAAGGCAACCAATCCGGACAA
GGCGCTCGATGCTCTCCCCTTCTACATGGTGGGCGAGGTGTGGGATCACGGGGTCGCCAAGGATTTCTGGTACCAGAATG
GCTTCGATTCCCTGATCAACTTCGACTATCAACGGGAGTTCGCCCTGCCCCAGGCCCAGTGCATGGCGGGCAGCGATCCC
ACCTATGCCAGCTACGCCAGCCGCATCAATCAGGATCCCGAGTTCAACGTGCTGAGCTACATCAGCTCCCACGACACCAA
GCTGTTCTTTGGTGACTATCAGGACGTGCCGCTGCAACGCAGAGTCGCCAACAGCTTCATGCTGCTGCCGGGGGGCATCC
AGCTCTACTACGGCGATGGGTCTGGCCAAGGATGGCGGCGTGTTCGATCAGAGTGTTCGCTCGGACATGAACTGGCAGGA
GATCGGGAGCGGCGAGAAGGCCGAACTGGTCAAGCACTGGCAGCGACTCGGCCAGTTCCGCGCCGCCCATCCGGCCATAG
CCGCTGGCAGCCACAAGAAGCTGTCCGATGCTCCCTACAGCTTCGTGCGGGAGAAGGGGGAGGACAAGGTGGTGGTGGTG
TTTGCCGGTCGGCAGCCGTAGACCTTCCCACGCATCTGCAGAGAGGGGAGCCAATGGCTCCCCTCTTCAATGTCAGTCGC
CGACCCGTTCAACAGGACCGTCTTACGTCGGTTGACGGGCAGCCACAGGCCACTGCAACCGTACCTTGA

Upstream 100 bases:

>100_bases
ACAGATCGGCGTTTTGCCTGTCCAAGCCAGCCACTATGCCCCCTAGAATGAGCCCCATTGACGGAATCTGGTCCGGTTAC
CCTTGCTCAAGGAATGGCTT

Downstream 100 bases:

>100_bases
CCAGCAGAGCCTGCCACAAAGGCCAGGCCAGCAAGGCCTGCTGATAGACACCGGCTTGACCGGGCAACTGGATGCCATAG
CTGGCCAGCCGATAGGCTAT

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MRRSPRFSPLWLTLLAGPLCSGLVLASPEVHLYVDGKPVAEVLTLDKGTVSLTHKLDKGSHQIRIGDAGNSCGTSFGPTE
AKPLPFGTAQPMDKCAKGTSFTLRVMLAGDYEFTFNPDTPSLKVLRATRKSEFKRQPPTEPCIAWDGGPVTVSLKGAWPD
GTRLRDAYSKQEAVVTQGKLTLTPSKESGGLLLLEPVKAAKQAPFSWDQASVYFLLTDRFHNGDPANDHSFGRQKDGQDE
VATWHGGDFKGLTEKLDYIKSLGMNAIWITPMVEQVHGFIGGGEQGNFPFYAYHGYWALDFTKIDPNYGDEESLRTLVDE
AHKRGMRIILDVVMNHAGYATLADLQDLGLTDLTQNTGKLPATWNQLRPSGGLNWHGYNQFIDYQSPDWNKWWSGDWVRA
GLPGYPQPGTDDVTGSMAGLPDFLTESTKPVGLPPLLAAKKDTRAKALPNATVSDYLIAWHTDWVRRFGIDGFRADTVKH
LEPAVWAKLKQAGTAALKEWKATNPDKALDALPFYMVGEVWDHGVAKDFWYQNGFDSLINFDYQREFALPQAQCMAGSDP
TYASYASRINQDPEFNVLSYISSHDTKLFFGDYQDVPLQRRVANSFMLLPGGIQLYYGDGSGQGWRRVRSECSLGHELAG
DRERREGRTGQALAATRPVPRRPSGHSRWQPQEAVRCSLQLRAGEGGGQGGGGVCRSAAVDLPTHLQRGEPMAPLFNVSR
RPVQQDRLTSVDGQPQATATVP

Sequences:

>Translated_742_residues
MRRSPRFSPLWLTLLAGPLCSGLVLASPEVHLYVDGKPVAEVLTLDKGTVSLTHKLDKGSHQIRIGDAGNSCGTSFGPTE
AKPLPFGTAQPMDKCAKGTSFTLRVMLAGDYEFTFNPDTPSLKVLRATRKSEFKRQPPTEPCIAWDGGPVTVSLKGAWPD
GTRLRDAYSKQEAVVTQGKLTLTPSKESGGLLLLEPVKAAKQAPFSWDQASVYFLLTDRFHNGDPANDHSFGRQKDGQDE
VATWHGGDFKGLTEKLDYIKSLGMNAIWITPMVEQVHGFIGGGEQGNFPFYAYHGYWALDFTKIDPNYGDEESLRTLVDE
AHKRGMRIILDVVMNHAGYATLADLQDLGLTDLTQNTGKLPATWNQLRPSGGLNWHGYNQFIDYQSPDWNKWWSGDWVRA
GLPGYPQPGTDDVTGSMAGLPDFLTESTKPVGLPPLLAAKKDTRAKALPNATVSDYLIAWHTDWVRRFGIDGFRADTVKH
LEPAVWAKLKQAGTAALKEWKATNPDKALDALPFYMVGEVWDHGVAKDFWYQNGFDSLINFDYQREFALPQAQCMAGSDP
TYASYASRINQDPEFNVLSYISSHDTKLFFGDYQDVPLQRRVANSFMLLPGGIQLYYGDGSGQGWRRVRSECSLGHELAG
DRERREGRTGQALAATRPVPRRPSGHSRWQPQEAVRCSLQLRAGEGGGQGGGGVCRSAAVDLPTHLQRGEPMAPLFNVSR
RPVQQDRLTSVDGQPQATATVP
>Mature_742_residues
MRRSPRFSPLWLTLLAGPLCSGLVLASPEVHLYVDGKPVAEVLTLDKGTVSLTHKLDKGSHQIRIGDAGNSCGTSFGPTE
AKPLPFGTAQPMDKCAKGTSFTLRVMLAGDYEFTFNPDTPSLKVLRATRKSEFKRQPPTEPCIAWDGGPVTVSLKGAWPD
GTRLRDAYSKQEAVVTQGKLTLTPSKESGGLLLLEPVKAAKQAPFSWDQASVYFLLTDRFHNGDPANDHSFGRQKDGQDE
VATWHGGDFKGLTEKLDYIKSLGMNAIWITPMVEQVHGFIGGGEQGNFPFYAYHGYWALDFTKIDPNYGDEESLRTLVDE
AHKRGMRIILDVVMNHAGYATLADLQDLGLTDLTQNTGKLPATWNQLRPSGGLNWHGYNQFIDYQSPDWNKWWSGDWVRA
GLPGYPQPGTDDVTGSMAGLPDFLTESTKPVGLPPLLAAKKDTRAKALPNATVSDYLIAWHTDWVRRFGIDGFRADTVKH
LEPAVWAKLKQAGTAALKEWKATNPDKALDALPFYMVGEVWDHGVAKDFWYQNGFDSLINFDYQREFALPQAQCMAGSDP
TYASYASRINQDPEFNVLSYISSHDTKLFFGDYQDVPLQRRVANSFMLLPGGIQLYYGDGSGQGWRRVRSECSLGHELAG
DRERREGRTGQALAATRPVPRRPSGHSRWQPQEAVRCSLQLRAGEGGGQGGGGVCRSAAVDLPTHLQRGEPMAPLFNVSR
RPVQQDRLTSVDGQPQATATVP

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=527, Percent_Identity=46.6793168880455, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1786604, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790687, Length=91, Percent_Identity=39.5604395604396, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=92, Percent_Identity=35.8695652173913, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=92, Percent_Identity=39.1304347826087, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=92, Percent_Identity=39.1304347826087, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=92, Percent_Identity=35.8695652173913, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322241, Length=92, Percent_Identity=35.8695652173913, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=92, Percent_Identity=35.8695652173913, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=92, Percent_Identity=35.8695652173913, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 81801; Mature: 81801

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSPRFSPLWLTLLAGPLCSGLVLASPEVHLYVDGKPVAEVLTLDKGTVSLTHKLDKGS
CCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHEECCCCCEEEEEEECCCC
HQIRIGDAGNSCGTSFGPTEAKPLPFGTAQPMDKCAKGTSFTLRVMLAGDYEFTFNPDTP
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCEEEEECCCCC
SLKVLRATRKSEFKRQPPTEPCIAWDGGPVTVSLKGAWPDGTRLRDAYSKQEAVVTQGKL
CHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCEE
TLTPSKESGGLLLLEPVKAAKQAPFSWDQASVYFLLTDRFHNGDPANDHSFGRQKDGQDE
EECCCCCCCCEEEECCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
VATWHGGDFKGLTEKLDYIKSLGMNAIWITPMVEQVHGFIGGGEQGNFPFYAYHGYWALD
EEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
FTKIDPNYGDEESLRTLVDEAHKRGMRIILDVVMNHAGYATLADLQDLGLTDLTQNTGKL
EEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCHHHCCCCCCC
PATWNQLRPSGGLNWHGYNQFIDYQSPDWNKWWSGDWVRAGLPGYPQPGTDDVTGSMAGL
CCCHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PDFLTESTKPVGLPPLLAAKKDTRAKALPNATVSDYLIAWHTDWVRRFGIDGFRADTVKH
CHHHHCCCCCCCCCCHHHCCCCCHHHCCCCCCHHHEEEEEEHHHHHHHCCCCCCHHHHHH
LEPAVWAKLKQAGTAALKEWKATNPDKALDALPFYMVGEVWDHGVAKDFWYQNGFDSLIN
HCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHC
FDYQREFALPQAQCMAGSDPTYASYASRINQDPEFNVLSYISSHDTKLFFGDYQDVPLQR
CCCHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHH
RVANSFMLLPGGIQLYYGDGSGQGWRRVRSECSLGHELAGDRERREGRTGQALAATRPVP
HHCCCEEEECCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCHHHHCCCCCCEEECCCCCC
RRPSGHSRWQPQEAVRCSLQLRAGEGGGQGGGGVCRSAAVDLPTHLQRGEPMAPLFNVSR
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHCCCC
RPVQQDRLTSVDGQPQATATVP
CCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRSPRFSPLWLTLLAGPLCSGLVLASPEVHLYVDGKPVAEVLTLDKGTVSLTHKLDKGS
CCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHEECCCCCEEEEEEECCCC
HQIRIGDAGNSCGTSFGPTEAKPLPFGTAQPMDKCAKGTSFTLRVMLAGDYEFTFNPDTP
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCEEEEECCCCC
SLKVLRATRKSEFKRQPPTEPCIAWDGGPVTVSLKGAWPDGTRLRDAYSKQEAVVTQGKL
CHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCEE
TLTPSKESGGLLLLEPVKAAKQAPFSWDQASVYFLLTDRFHNGDPANDHSFGRQKDGQDE
EECCCCCCCCEEEECCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
VATWHGGDFKGLTEKLDYIKSLGMNAIWITPMVEQVHGFIGGGEQGNFPFYAYHGYWALD
EEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
FTKIDPNYGDEESLRTLVDEAHKRGMRIILDVVMNHAGYATLADLQDLGLTDLTQNTGKL
EEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCHHHCCCCCCC
PATWNQLRPSGGLNWHGYNQFIDYQSPDWNKWWSGDWVRAGLPGYPQPGTDDVTGSMAGL
CCCHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PDFLTESTKPVGLPPLLAAKKDTRAKALPNATVSDYLIAWHTDWVRRFGIDGFRADTVKH
CHHHHCCCCCCCCCCHHHCCCCCHHHCCCCCCHHHEEEEEEHHHHHHHCCCCCCHHHHHH
LEPAVWAKLKQAGTAALKEWKATNPDKALDALPFYMVGEVWDHGVAKDFWYQNGFDSLIN
HCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHC
FDYQREFALPQAQCMAGSDPTYASYASRINQDPEFNVLSYISSHDTKLFFGDYQDVPLQR
CCCHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHH
RVANSFMLLPGGIQLYYGDGSGQGWRRVRSECSLGHELAGDRERREGRTGQALAATRPVP
HHCCCEEEECCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCHHHHCCCCCCEEECCCCCC
RRPSGHSRWQPQEAVRCSLQLRAGEGGGQGGGGVCRSAAVDLPTHLQRGEPMAPLFNVSR
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHCCCC
RPVQQDRLTSVDGQPQATATVP
CCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]