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The map label for this gene is pyk [H]

Identifier: 145299979

GI number: 145299979

Start: 3313963

End: 3315375

Strand: Direct

Name: pyk [H]

Synonym: ASA_3074

Alternate gene names: 145299979

Gene position: 3313963-3315375 (Clockwise)

Preceding gene: 145299978

Following gene: 145299980

Centisome position: 70.47

GC content: 57.54

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTCTGCACCATAGGTCCCAAGACCGAATCCAAAGAAATGCTGGCTAAAATGCTGGATGCCGG
CATGAACGTCATGCGCCTGAACTTCTCCCACGGCGATTACGCCGAGCACGGTGGTCGTATCAACAACCTGCGCGCTGTCA
TGGCCGAAACCGGCAAGCACGCAGCCATACTGCTCGACACCAAGGGTCCGGAAATCCGTACCATCAAGCTGGAAGGCGGC
AACGACGTTGCCCTGGTCGCTGGCCAGACCTTCACCTTCACCACCGACCAGACCGTCATTGGCAACAAAGACAAAGTGGC
CGTGACCTATGCCGGTTTCGCCAACGACCTGCGCGTCGGCAATCGCATCCTGGTGGATGACGGCCTGATCGGCATGGATG
TGATCGAAATCACCGAGCGTGAAGTCATCTGTAAAGTGCTGAACAGCGGTGATCTGGGCGAGAACAAAGGCATCAACCTG
CCGGGTGTCTCCATCAAGCTGCCGGCCCTGGCCGAGAAAGACAAGCGCGACCTCATCTTCGGTTGTGAGCAAGGCGTTGA
TTTCGTTGCGGCTTCCTTCATTCGCAAGAAAGAAGACGTGCTGGAAATCCGTGAGCACCTGAGAGCCCACGGTGGCGAGA
ACATCCAGATCATCTCCAAGATCGAAAACCAGGAAGGCCTGGACAACTTCGACGAGATCCTGGCTGTCTCCGACGGCATC
ATGGTGGCACGTGGCGACATGGGCGTGGAAATTCCGGTTGAAGAAGTGATCTTCGCCCAGAAGATGATCATCACCAAGTG
CAACAAGGCACGCAAAGTGGTGATCACCGCCACCCAGATGCTGGATTCCATGATCAAGAACCCGCGTCCGACCCGCGCCG
AAGCCGGCGACGTGGCCAACGCCATCCTGGACGGTACCGATGCAGTCATGCTGTCCGGTGAGTCCGCCAAGGGCAAGTAC
CCGCTGGAAGCCGTGACCATCATGGCCACCATCTGCGAGCGTACCGATGCCGTTATGCCGTCCAACCTGTCTGCTGCCAA
CGACAGCAACAAGCTGCGCATAACCGAAGCCGTTTGTAAAGGCGCGGTCGAGACCTCCGAGAAGCTGGGCGCCCCGCTGA
TCGTGGTTGCCACCGAAGGCGGCAAATCTGCCAAGGCCATTCGCAAGTACTTCCCGCAAGCCTGGATCCTGGCCATCACC
ACCAACAAGAAAACTGCAGCCCAACTGGTTCTGACCAAGGGTGTGGTGACTCACGTGGTTGACAGCATCGCCTCTACCGA
CGATTTCTATCGCATCGGTAAAGAAGCAGCGCTGGAAAGCGGCATGGGTCTGAAGGGTGACGTGGTCGTCATGGTCTCCG
GTGCTCTGGTACCGAGCGGTACCACCAACACCGCCTCCGTTCACGTACTCTAA

Upstream 100 bases:

>100_bases
GAAAACGTTATCTGGTCAAAACGTTGTTTCTGGATCATAAAAAGTATCCAAAAACTGGTGTATATTGCACGGCGGAAACT
CCTTAACCATGAAGACTGAC

Downstream 100 bases:

>100_bases
TCAGTCCTGAACCGATAAAACGGCGCCTGCGGGCGCCGTTTTTTTTATCTGCCCCCCTGCTTCCCGTTCAAGCCCGGTGT
TAGAATCCACGCGGATTCAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESKEMLAKMLDAGMNVMRLNFSHGDYAEHGGRINNLRAVMAETGKHAAILLDTKGPEIRTIKLEGG
NDVALVAGQTFTFTTDQTVIGNKDKVAVTYAGFANDLRVGNRILVDDGLIGMDVIEITEREVICKVLNSGDLGENKGINL
PGVSIKLPALAEKDKRDLIFGCEQGVDFVAASFIRKKEDVLEIREHLRAHGGENIQIISKIENQEGLDNFDEILAVSDGI
MVARGDMGVEIPVEEVIFAQKMIITKCNKARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDAVMPSNLSAANDSNKLRITEAVCKGAVETSEKLGAPLIVVATEGGKSAKAIRKYFPQAWILAIT
TNKKTAAQLVLTKGVVTHVVDSIASTDDFYRIGKEAALESGMGLKGDVVVMVSGALVPSGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESKEMLAKMLDAGMNVMRLNFSHGDYAEHGGRINNLRAVMAETGKHAAILLDTKGPEIRTIKLEGG
NDVALVAGQTFTFTTDQTVIGNKDKVAVTYAGFANDLRVGNRILVDDGLIGMDVIEITEREVICKVLNSGDLGENKGINL
PGVSIKLPALAEKDKRDLIFGCEQGVDFVAASFIRKKEDVLEIREHLRAHGGENIQIISKIENQEGLDNFDEILAVSDGI
MVARGDMGVEIPVEEVIFAQKMIITKCNKARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDAVMPSNLSAANDSNKLRITEAVCKGAVETSEKLGAPLIVVATEGGKSAKAIRKYFPQAWILAIT
TNKKTAAQLVLTKGVVTHVVDSIASTDDFYRIGKEAALESGMGLKGDVVVMVSGALVPSGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESKEMLAKMLDAGMNVMRLNFSHGDYAEHGGRINNLRAVMAETGKHAAILLDTKGPEIRTIKLEGG
NDVALVAGQTFTFTTDQTVIGNKDKVAVTYAGFANDLRVGNRILVDDGLIGMDVIEITEREVICKVLNSGDLGENKGINL
PGVSIKLPALAEKDKRDLIFGCEQGVDFVAASFIRKKEDVLEIREHLRAHGGENIQIISKIENQEGLDNFDEILAVSDGI
MVARGDMGVEIPVEEVIFAQKMIITKCNKARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDAVMPSNLSAANDSNKLRITEAVCKGAVETSEKLGAPLIVVATEGGKSAKAIRKYFPQAWILAIT
TNKKTAAQLVLTKGVVTHVVDSIASTDDFYRIGKEAALESGMGLKGDVVVMVSGALVPSGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=46.3114754098361, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=46.3114754098361, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=46.2012320328542, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=45.7905544147844, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=45.7905544147844, Blast_Score=371, Evalue=1e-102,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=48.9583333333333, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=48.9583333333333, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=45.3389830508475, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=45.3389830508475, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=45.1162790697674, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=83.4042553191489, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=37.7358490566038, Blast_Score=276, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=45.7731958762887, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=45.7731958762887, Blast_Score=347, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=45.7731958762887, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=45.7731958762887, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=40.6122448979592, Blast_Score=341, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=44.0748440748441, Blast_Score=342, Evalue=6e-95,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=43.298969072165, Blast_Score=340, Evalue=4e-94,
Organism=Drosophila melanogaster, GI28571814, Length=479, Percent_Identity=47.1816283924843, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648964, Length=479, Percent_Identity=47.1816283924843, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24648966, Length=444, Percent_Identity=46.8468468468468, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=34.7193347193347, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24646914, Length=304, Percent_Identity=42.7631578947368, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50279; Mature: 50279

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESKEMLAKMLDAGMNVMRLNFSHGDYAEHGGRINNLRAVMAETGKH
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCE
AAILLDTKGPEIRTIKLEGGNDVALVAGQTFTFTTDQTVIGNKDKVAVTYAGFANDLRVG
EEEEEECCCCEEEEEEEECCCEEEEEECCEEEEECCCEEECCCCEEEEEEECCCCCCCCC
NRILVDDGLIGMDVIEITEREVICKVLNSGDLGENKGINLPGVSIKLPALAEKDKRDLIF
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKKEDVLEIREHLRAHGGENIQIISKIENQEGLDNFDEILAVSDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHEECCCE
MVARGDMGVEIPVEEVIFAQKMIITKCNKARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICERTDAVMPSNLSAANDSNKLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHC
GAVETSEKLGAPLIVVATEGGKSAKAIRKYFPQAWILAITTNKKTAAQLVLTKGVVTHVV
CCHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
DSIASTDDFYRIGKEAALESGMGLKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESKEMLAKMLDAGMNVMRLNFSHGDYAEHGGRINNLRAVMAETGKH
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCE
AAILLDTKGPEIRTIKLEGGNDVALVAGQTFTFTTDQTVIGNKDKVAVTYAGFANDLRVG
EEEEEECCCCEEEEEEEECCCEEEEEECCEEEEECCCEEECCCCEEEEEEECCCCCCCCC
NRILVDDGLIGMDVIEITEREVICKVLNSGDLGENKGINLPGVSIKLPALAEKDKRDLIF
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKKEDVLEIREHLRAHGGENIQIISKIENQEGLDNFDEILAVSDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHEECCCE
MVARGDMGVEIPVEEVIFAQKMIITKCNKARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICERTDAVMPSNLSAANDSNKLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHC
GAVETSEKLGAPLIVVATEGGKSAKAIRKYFPQAWILAITTNKKTAAQLVLTKGVVTHVV
CCHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
DSIASTDDFYRIGKEAALESGMGLKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]