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The map label for this gene is cysK [H]

Identifier: 145299965

GI number: 145299965

Start: 3293610

End: 3294578

Strand: Direct

Name: cysK [H]

Synonym: ASA_3059

Alternate gene names: 145299965

Gene position: 3293610-3294578 (Clockwise)

Preceding gene: 145299961

Following gene: 145299966

Centisome position: 70.04

GC content: 60.47

Gene sequence:

>969_bases
ATGAGCAAAATTTTTGAGGACAACAGCCAGACCATAGGTCACACCCCGCTGGTTCGACTCAACCGTGTTACCAAGGGCCG
CGTACTGGCCAAGATTGAAAGCCGCAACCCGAGCTTCAGCGTCAAGTGCCGTATCGGTGCCAACCTGATCTGGGATGCCG
AGAAGCGCGGCGTACTGACCCAGGGCAAGGAGATCATCGAGCCCACCAGTGGTAACACAGGTATTGCTCTGGCATTTGTG
GCCGCCGCCCGTGGCTATCCCATCACCCTGACCATGCCGGCCACCATGAGCCTGGAGCGCCGCAAGCTGCTCAAGGCGCT
CGGTGCCAATCTGGTGCTGACCGAAGGCCCTCTTGGCATGAAAGGCGCTATCGCCAAGGCCAACGAGATCCTCGAATCCG
AGCCGGGCAGATACGTTTTGCTGCAACAGTTTGAAAACCCCGCCAACCCGGAAATTCACGAGAAGACCACTGGCCCCGAG
ATCTGGGATGCCACCGACGGTCAGGTGGATGTATTCGTGGCAGGTGTAGGTACAGGTGGCACCATTACCGGCGTCTCCCG
CTACCTGAAGCAGACCAAAGGCAAGGCCGTGATCTCGGTGGCCGTCGAGCCAAGCGAATCACCGGTGATCAGCCAGCGTC
TGGCCGGTCTCGAAATCAAACCGGGTCCCCACAAGATCCAAGGCATTGGTGCCGGCTTCATCCCGGGCAACCTGGACCTG
ACCCTGCTGGACCGCGTCGAACAGGTGAGCAGCGATGATGCCATCGAGATGGCGCGCCGTCTGATGGAAGAGGAAGGCAT
TCTGGCCGGTATCAGCTCTGGAGCCGCCGTCGTGGCCGCCGCCCGTCTGGCCGAGCTGCCCGAGTTTGCCGACAAGACAA
TCGTCGTCATACTGCCGAGCGCTGCCGAGCGTTATCTCTCCAGCGCGCTGTTTGCCGGCGTGTTCAGCGAGCAGGAGTTG
CAACAGTAA

Upstream 100 bases:

>100_bases
TATATCTTGTAATAACATATTGTTATAACAAATCGTCACTTCTCATTCAGAAACCACAGGGTAATCTTGTTTCATCGCCG
TAACAAACAGGGATAGCACC

Downstream 100 bases:

>100_bases
GTGATCGGATAATTTCAAAACAACGGCGCTCCCAGGAGCGCCGTTTCTTTATGAGCTAGACTGAGCACTTGGCAGCGCCA
CTCGGCCCCTTATTTTTTGG

Product: cysteine synthase

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIFEDNSQTIGHTPLVRLNRVTKGRVLAKIESRNPSFSVKCRIGANLIWDAEKRGVLTQGKEIIEPTSGNTGIALAFV
AAARGYPITLTMPATMSLERRKLLKALGANLVLTEGPLGMKGAIAKANEILESEPGRYVLLQQFENPANPEIHEKTTGPE
IWDATDGQVDVFVAGVGTGGTITGVSRYLKQTKGKAVISVAVEPSESPVISQRLAGLEIKPGPHKIQGIGAGFIPGNLDL
TLLDRVEQVSSDDAIEMARRLMEEEGILAGISSGAAVVAAARLAELPEFADKTIVVILPSAAERYLSSALFAGVFSEQEL
QQ

Sequences:

>Translated_322_residues
MSKIFEDNSQTIGHTPLVRLNRVTKGRVLAKIESRNPSFSVKCRIGANLIWDAEKRGVLTQGKEIIEPTSGNTGIALAFV
AAARGYPITLTMPATMSLERRKLLKALGANLVLTEGPLGMKGAIAKANEILESEPGRYVLLQQFENPANPEIHEKTTGPE
IWDATDGQVDVFVAGVGTGGTITGVSRYLKQTKGKAVISVAVEPSESPVISQRLAGLEIKPGPHKIQGIGAGFIPGNLDL
TLLDRVEQVSSDDAIEMARRLMEEEGILAGISSGAAVVAAARLAELPEFADKTIVVILPSAAERYLSSALFAGVFSEQEL
QQ
>Mature_321_residues
SKIFEDNSQTIGHTPLVRLNRVTKGRVLAKIESRNPSFSVKCRIGANLIWDAEKRGVLTQGKEIIEPTSGNTGIALAFVA
AARGYPITLTMPATMSLERRKLLKALGANLVLTEGPLGMKGAIAKANEILESEPGRYVLLQQFENPANPEIHEKTTGPEI
WDATDGQVDVFVAGVGTGGTITGVSRYLKQTKGKAVISVAVEPSESPVISQRLAGLEIKPGPHKIQGIGAGFIPGNLDLT
LLDRVEQVSSDDAIEMARRLMEEEGILAGISSGAAVVAAARLAELPEFADKTIVVILPSAAERYLSSALFAGVFSEQELQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=321, Percent_Identity=39.8753894080997, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI295821200, Length=321, Percent_Identity=39.8753894080997, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI4557415, Length=321, Percent_Identity=39.8753894080997, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=77.7089783281734, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI2367138, Length=316, Percent_Identity=42.4050632911392, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=313, Percent_Identity=52.0766773162939, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17562970, Length=318, Percent_Identity=48.4276729559748, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=317, Percent_Identity=55.205047318612, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=49.5114006514658, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17561720, Length=318, Percent_Identity=48.7421383647799, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=49.5575221238938, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=40.3785488958991, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=324, Percent_Identity=36.1111111111111, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=50.381679389313, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=36.7088607594937, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=36.1111111111111, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24643623, Length=306, Percent_Identity=37.5816993464052, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI20129101, Length=306, Percent_Identity=37.5816993464052, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34306; Mature: 34175

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFEDNSQTIGHTPLVRLNRVTKGRVLAKIESRNPSFSVKCRIGANLIWDAEKRGVLT
CCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCHH
QGKEIIEPTSGNTGIALAFVAAARGYPITLTMPATMSLERRKLLKALGANLVLTEGPLGM
CCHHHCCCCCCCCCEEEEEEHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKANEILESEPGRYVLLQQFENPANPEIHEKTTGPEIWDATDGQVDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
TITGVSRYLKQTKGKAVISVAVEPSESPVISQRLAGLEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCEECCCCCEEEEECCCCCCCCCCC
TLLDRVEQVSSDDAIEMARRLMEEEGILAGISSGAAVVAAARLAELPEFADKTIVVILPS
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCHHHCCCEEEEEECC
AAERYLSSALFAGVFSEQELQQ
HHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
SKIFEDNSQTIGHTPLVRLNRVTKGRVLAKIESRNPSFSVKCRIGANLIWDAEKRGVLT
CCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCHH
QGKEIIEPTSGNTGIALAFVAAARGYPITLTMPATMSLERRKLLKALGANLVLTEGPLGM
CCHHHCCCCCCCCCEEEEEEHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKANEILESEPGRYVLLQQFENPANPEIHEKTTGPEIWDATDGQVDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
TITGVSRYLKQTKGKAVISVAVEPSESPVISQRLAGLEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCEECCCCCEEEEECCCCCCCCCCC
TLLDRVEQVSSDDAIEMARRLMEEEGILAGISSGAAVVAAARLAELPEFADKTIVVILPS
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCHHHCCCEEEEEECC
AAERYLSSALFAGVFSEQELQQ
HHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]