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The map label for this gene is malI [H]

Identifier: 145299862

GI number: 145299862

Start: 3173558

End: 3174595

Strand: Direct

Name: malI [H]

Synonym: ASA_2952

Alternate gene names: 145299862

Gene position: 3173558-3174595 (Clockwise)

Preceding gene: 145299859

Following gene: 145299863

Centisome position: 67.49

GC content: 64.74

Gene sequence:

>1038_bases
ATGTCGTCCAAACCGCTTGTATCGAAAAACGTCAAGATCACCGATGTCGCCCAGCTGGCGGGAGTCTCGGTCACTACCGT
CTCCATGGTGCTCAATGGCAAGGGGCGTATCTCGCCGGCGACCGCCGAGCGGGTTCAGCAGGCCATCAAAGCGCTCGACT
ACGTGCCCAACAGCGCCGCCGCCAACCTGCGCAGCCAGCAATCCAACCTGGTGGGACTGATCCTGCGCGACATCACGGAT
CCCTTCTATACCGAGGTCACGGCCGGGGTGAGCGAGGTGCTGGAGCAACAGGGCTATCTGCTGTTTCTGACCCAGTGCGG
CCACAGCCCGGAGCGATTGCAGCAGAGCATCCAGTCCCTGTCACGCCAGGGGGTGGCGGGCATCATCTTCAATCCGGTGC
GCGGCGCGGCGGCTAAAACCCTGGAGACCCTGCTGGGCTCACAGCTGCCGGTGGTGTGCGCCGCCCGCTCCTACTACCGG
GATGACGTGGACTTTATCGGTCCCGACAACACCCATGCCGCCCAGCAGGCCACCACCTATCTCATCGAGCAGGGCCACCG
CCATATCGCCTACGTGGGCGGGCGGGCCGACTCCCTCACCCGCGCCGAGCGCATCGGCGGCTACTGCGCCAGCCTGCTGC
AATACGGCCTCCCCTTCAAACCCGAGTGGGTACTGGAGTGCGAGCGCACCCAGCGCAGCGCAGCCGACACTATCGCCCAA
CTGCTGCACCAGCACCCCAAGGTGACGGCCATTCTCTGCCACTACCCAGAGGTGGCGCTCGGCGGCATTTACGGGGTGGA
GGCGAGCGGACGCACAGTGGGCAAGGACAACTACATAGGCCAGCAAGTAGCCCTGCTCGGCTTTGACGACGTGGCAGAGG
CGGAACTCACTTATCCCGCCCTCACCTTCGTCAGCTCACCGGCACGGGAAATAGGCCGCCAGGCCGCCTTGCGGCTGCTG
ACCCGGATGCAGCAACCGGAGCTTGCCCCCAATCGTCATATCATCGCCCCCCATCTGCAGAAGCGGGCATCAGCCTGA

Upstream 100 bases:

>100_bases
TCACTTGGGTATACGAGGGGGTATGCTGTGGTTCTCCGGTCTCTTTGCCCATGGCTGCAGCACTCGCACTATGTCGTCAC
AACCCATGCCGTCCAAACCT

Downstream 100 bases:

>100_bases
CCAGGCAGGTCGCGCCATGCGGCCTCGCCTTGTCATGTCCCCCACCCCCCCAGATGCCGGGTGCCATCAACAAACAGGCC
ATTGGATGATCGGGAACCGA

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSSKPLVSKNVKITDVAQLAGVSVTTVSMVLNGKGRISPATAERVQQAIKALDYVPNSAAANLRSQQSNLVGLILRDITD
PFYTEVTAGVSEVLEQQGYLLFLTQCGHSPERLQQSIQSLSRQGVAGIIFNPVRGAAAKTLETLLGSQLPVVCAARSYYR
DDVDFIGPDNTHAAQQATTYLIEQGHRHIAYVGGRADSLTRAERIGGYCASLLQYGLPFKPEWVLECERTQRSAADTIAQ
LLHQHPKVTAILCHYPEVALGGIYGVEASGRTVGKDNYIGQQVALLGFDDVAEAELTYPALTFVSSPAREIGRQAALRLL
TRMQQPELAPNRHIIAPHLQKRASA

Sequences:

>Translated_345_residues
MSSKPLVSKNVKITDVAQLAGVSVTTVSMVLNGKGRISPATAERVQQAIKALDYVPNSAAANLRSQQSNLVGLILRDITD
PFYTEVTAGVSEVLEQQGYLLFLTQCGHSPERLQQSIQSLSRQGVAGIIFNPVRGAAAKTLETLLGSQLPVVCAARSYYR
DDVDFIGPDNTHAAQQATTYLIEQGHRHIAYVGGRADSLTRAERIGGYCASLLQYGLPFKPEWVLECERTQRSAADTIAQ
LLHQHPKVTAILCHYPEVALGGIYGVEASGRTVGKDNYIGQQVALLGFDDVAEAELTYPALTFVSSPAREIGRQAALRLL
TRMQQPELAPNRHIIAPHLQKRASA
>Mature_344_residues
SSKPLVSKNVKITDVAQLAGVSVTTVSMVLNGKGRISPATAERVQQAIKALDYVPNSAAANLRSQQSNLVGLILRDITDP
FYTEVTAGVSEVLEQQGYLLFLTQCGHSPERLQQSIQSLSRQGVAGIIFNPVRGAAAKTLETLLGSQLPVVCAARSYYRD
DVDFIGPDNTHAAQQATTYLIEQGHRHIAYVGGRADSLTRAERIGGYCASLLQYGLPFKPEWVLECERTQRSAADTIAQL
LHQHPKVTAILCHYPEVALGGIYGVEASGRTVGKDNYIGQQVALLGFDDVAEAELTYPALTFVSSPAREIGRQAALRLLT
RMQQPELAPNRHIIAPHLQKRASA

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=44.7447447447448, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1790369, Length=313, Percent_Identity=28.7539936102236, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=28.6549707602339, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=28.4375, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=29.375, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=28.6144578313253, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787580, Length=341, Percent_Identity=29.3255131964809, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=27.3615635179153, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=23.8390092879257, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=25.8720930232558, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786268, Length=167, Percent_Identity=28.7425149700599, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=24.9230769230769, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37384; Mature: 37253

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKPLVSKNVKITDVAQLAGVSVTTVSMVLNGKGRISPATAERVQQAIKALDYVPNSAA
CCCCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHH
ANLRSQQSNLVGLILRDITDPFYTEVTAGVSEVLEQQGYLLFLTQCGHSPERLQQSIQSL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
SRQGVAGIIFNPVRGAAAKTLETLLGSQLPVVCAARSYYRDDVDFIGPDNTHAAQQATTY
HHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LIEQGHRHIAYVGGRADSLTRAERIGGYCASLLQYGLPFKPEWVLECERTQRSAADTIAQ
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
LLHQHPKVTAILCHYPEVALGGIYGVEASGRTVGKDNYIGQQVALLGFDDVAEAELTYPA
HHHCCCCEEEEEEECCCHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
LTFVSSPAREIGRQAALRLLTRMQQPELAPNRHIIAPHLQKRASA
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHCCC
>Mature Secondary Structure 
SSKPLVSKNVKITDVAQLAGVSVTTVSMVLNGKGRISPATAERVQQAIKALDYVPNSAA
CCCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHH
ANLRSQQSNLVGLILRDITDPFYTEVTAGVSEVLEQQGYLLFLTQCGHSPERLQQSIQSL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
SRQGVAGIIFNPVRGAAAKTLETLLGSQLPVVCAARSYYRDDVDFIGPDNTHAAQQATTY
HHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LIEQGHRHIAYVGGRADSLTRAERIGGYCASLLQYGLPFKPEWVLECERTQRSAADTIAQ
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
LLHQHPKVTAILCHYPEVALGGIYGVEASGRTVGKDNYIGQQVALLGFDDVAEAELTYPA
HHHCCCCEEEEEEECCCHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
LTFVSSPAREIGRQAALRLLTRMQQPELAPNRHIIAPHLQKRASA
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]