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The map label for this gene is dapE [H]

Identifier: 145299806

GI number: 145299806

Start: 3112058

End: 3113185

Strand: Direct

Name: dapE [H]

Synonym: ASA_2887

Alternate gene names: 145299806

Gene position: 3112058-3113185 (Clockwise)

Preceding gene: 145299805

Following gene: 145299807

Centisome position: 66.18

GC content: 62.94

Gene sequence:

>1128_bases
ATGTCGGACGTCATCGCACTGGCCAAGGATCTGATCCGCCGCCCCTCCGTTACCCCGCAGGATGAAGGGTGTCAGACCCT
GATGAGCGAACGCCTGGCGCGTCTTGGCTTCGTCATCGAACCCATGGTGTTTGAAGACACCACCAACCTCTGGGCGCGCC
GTGGCAGCGAAGGCCCCCTGTTCTGCTTCGCCGGCCACACCGATGTGGTACCGGCCGGGCCGCTGGAGAAGTGGCACACA
CCGCCGTTCGAACCGACCATCCAGGATGGCGTGCTCTACGGTCGCGGCGCCGCCGACATGAAGGGATCCCTCGCCGCCAT
GGTGGTGGCCGTCGAACGCTTCGTGGCCGAGCATCCGGATCACAAGGGGTCCATCGCCTTTCTCATCACCTCGGATGAAG
AGGGCCCCTTCATCAATGGCACAGTGCGTGTCATCGACACCCTTGAAGCGCGCAACGAGAAGATCCGCTGGTGCATCGTC
GGCGAGCCCTCCTCCACCCATGTGGTGGGGGACGTGGTAAAGAACGGTCGACGTGGCTCCATCACAGGTGATCTGCTGGT
ACGTGGCGTGCAGGGGCATGTGGCCTATCCCCATCTGGCGGACAACCCCATCCACAAGGCGGCGCCTGCATTGACTGAGC
TCGCCGCGACGGTATGGGATGAAGGCAACGCCTACTTCCCCCCCACCAGCTTCCAGATTGCCAATATCCAGGCCGGCACC
GGCGCCTCCAACGTCATTCCGGGGGAGTTGCAGGTTCAATTCAACTTCCGCTTCAGCACACAACTGACCGACATGGATAT
TCGTGAACGCGTCCAGGCCCTGCTCGACAAACACGGTCTGGACTACGAGCTGACATGGACCCTCTCCGGCCAGCCCTTCC
TGACCGATACCGGCGCCCTGCTGGAGGCCACAGTGGCGGCGGTAGCGGCGGTCAACGGCCAGCAACCGGCCCTGCTCACC
ACGGGCGGCACCTCGGATGGGCGCTTTATTGCCCCCACAGGGGCCGAAGTGATCGAACTCGGGCCCGTCAATGCCACCAT
CCACAAGGTGAACGAGTGCGTGAAGGCCGAGGATCTGGATCTGCTGGCAGAGATGTACCAAGGGGTACTCGAGAGATTGC
TGGCATGA

Upstream 100 bases:

>100_bases
AAGCGCCCGCTGCTGGATCGGGACGGCGAGCTGACCCTGGGTTTCAAGGCTGATCACTATCAATCCCTCTTCTCTCGTTA
AACTTTTTTAAGGAACGCTG

Downstream 100 bases:

>100_bases
CACAGGAACAACTGCTGGGGCTCGACGAGAGTCACCTTGTTCTGGTGGGACGCGGGCCCCATCGCCTGACCGCTGCCACT
GCAGCAGCCTTCAACGATAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSDVIALAKDLIRRPSVTPQDEGCQTLMSERLARLGFVIEPMVFEDTTNLWARRGSEGPLFCFAGHTDVVPAGPLEKWHT
PPFEPTIQDGVLYGRGAADMKGSLAAMVVAVERFVAEHPDHKGSIAFLITSDEEGPFINGTVRVIDTLEARNEKIRWCIV
GEPSSTHVVGDVVKNGRRGSITGDLLVRGVQGHVAYPHLADNPIHKAAPALTELAATVWDEGNAYFPPTSFQIANIQAGT
GASNVIPGELQVQFNFRFSTQLTDMDIRERVQALLDKHGLDYELTWTLSGQPFLTDTGALLEATVAAVAAVNGQQPALLT
TGGTSDGRFIAPTGAEVIELGPVNATIHKVNECVKAEDLDLLAEMYQGVLERLLA

Sequences:

>Translated_375_residues
MSDVIALAKDLIRRPSVTPQDEGCQTLMSERLARLGFVIEPMVFEDTTNLWARRGSEGPLFCFAGHTDVVPAGPLEKWHT
PPFEPTIQDGVLYGRGAADMKGSLAAMVVAVERFVAEHPDHKGSIAFLITSDEEGPFINGTVRVIDTLEARNEKIRWCIV
GEPSSTHVVGDVVKNGRRGSITGDLLVRGVQGHVAYPHLADNPIHKAAPALTELAATVWDEGNAYFPPTSFQIANIQAGT
GASNVIPGELQVQFNFRFSTQLTDMDIRERVQALLDKHGLDYELTWTLSGQPFLTDTGALLEATVAAVAAVNGQQPALLT
TGGTSDGRFIAPTGAEVIELGPVNATIHKVNECVKAEDLDLLAEMYQGVLERLLA
>Mature_374_residues
SDVIALAKDLIRRPSVTPQDEGCQTLMSERLARLGFVIEPMVFEDTTNLWARRGSEGPLFCFAGHTDVVPAGPLEKWHTP
PFEPTIQDGVLYGRGAADMKGSLAAMVVAVERFVAEHPDHKGSIAFLITSDEEGPFINGTVRVIDTLEARNEKIRWCIVG
EPSSTHVVGDVVKNGRRGSITGDLLVRGVQGHVAYPHLADNPIHKAAPALTELAATVWDEGNAYFPPTSFQIANIQAGTG
ASNVIPGELQVQFNFRFSTQLTDMDIRERVQALLDKHGLDYELTWTLSGQPFLTDTGALLEATVAAVAAVNGQQPALLTT
GGTSDGRFIAPTGAEVIELGPVNATIHKVNECVKAEDLDLLAEMYQGVLERLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=66.9354838709677, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1790395, Length=269, Percent_Identity=27.5092936802974, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789236, Length=224, Percent_Identity=25.8928571428571, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40457; Mature: 40326

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVIALAKDLIRRPSVTPQDEGCQTLMSERLARLGFVIEPMVFEDTTNLWARRGSEGPL
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCEEECCCHHHHHHCCCCCCE
FCFAGHTDVVPAGPLEKWHTPPFEPTIQDGVLYGRGAADMKGSLAAMVVAVERFVAEHPD
EEECCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HKGSIAFLITSDEEGPFINGTVRVIDTLEARNEKIRWCIVGEPSSTHVVGDVVKNGRRGS
CCCCEEEEEECCCCCCEECCEEEEEEEHHCCCCEEEEEEEECCCCCCEEHHHHHCCCCCC
ITGDLLVRGVQGHVAYPHLADNPIHKAAPALTELAATVWDEGNAYFPPTSFQIANIQAGT
CCHHHHEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCC
GASNVIPGELQVQFNFRFSTQLTDMDIRERVQALLDKHGLDYELTWTLSGQPFLTDTGAL
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHH
LEATVAAVAAVNGQQPALLTTGGTSDGRFIAPTGAEVIELGPVNATIHKVNECVKAEDLD
HHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHH
LLAEMYQGVLERLLA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVIALAKDLIRRPSVTPQDEGCQTLMSERLARLGFVIEPMVFEDTTNLWARRGSEGPL
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCEEECCCHHHHHHCCCCCCE
FCFAGHTDVVPAGPLEKWHTPPFEPTIQDGVLYGRGAADMKGSLAAMVVAVERFVAEHPD
EEECCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HKGSIAFLITSDEEGPFINGTVRVIDTLEARNEKIRWCIVGEPSSTHVVGDVVKNGRRGS
CCCCEEEEEECCCCCCEECCEEEEEEEHHCCCCEEEEEEEECCCCCCEEHHHHHCCCCCC
ITGDLLVRGVQGHVAYPHLADNPIHKAAPALTELAATVWDEGNAYFPPTSFQIANIQAGT
CCHHHHEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCC
GASNVIPGELQVQFNFRFSTQLTDMDIRERVQALLDKHGLDYELTWTLSGQPFLTDTGAL
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHH
LEATVAAVAAVNGQQPALLTTGGTSDGRFIAPTGAEVIELGPVNATIHKVNECVKAEDLD
HHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHH
LLAEMYQGVLERLLA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA